-
[show abstract]
[hide abstract]
ABSTRACT: Overt and occult bleeding are the main indications for a wireless capsule endoscopy (WCE) study of the small bowel. Most published studies omit patients aged over 80.
To determine whether WCE is feasible in patients with overt or occult bleeding over age 80 and to define the spectrum of small bowel pathologies in this age group.
In a retrospective non-randomized tertiary care study, 60 patients at least 80 years or older (aged group) and 120 matched patients aged <80 years (younger group) with overt or occult bleeding (including iron deficiency anaemia) and no significant gastroscopic or colonoscopic findings underwent WCE.
Of the 180 patients, 46 (77%) patients in the aged group and 97 (81%) in the younger group successfully completed small bowel study (P = 0.51). There was no difference in gastric transit time and small bowel passing time between the two groups. More patients in the aged group (48 cases, 80%) than the younger group (56 cases, 47%) presented with small bowel angiodysplasias (P < 0.0001). Nevertheless, there was no difference between the two groups concerning ulcerative and neoplastic lesions. No patient presented with capsule impaction, but more patients in the aged group (35 cases, 58%) than in the younger group (10 cases, 8%) found the study difficult and tiresome (P < 0.001). Preparation validation was poor in 34% of small bowel lumen in the aged group and 19% in the younger group (P = 0.03).
WCE is feasible, although rather tiresome, in patients over the age of 80. Though the vast majority of patients older than 80 presented with angiodysplasias, there were no differences between the aged and younger groups in the presence of ulcerative lesions and polyps or tumors.
Digestive Endoscopy 05/2012; 24(3):154-8. · 1.19 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The role of genetic factors in the etiology of gastroesophageal reflux disease (GERD) is still uncertain.
To define whether the presence of reflux symptoms in first-degree relatives can affect the severity of the endoscopic picture of patients with GERD and disease evolution during follow-up.
A total of 1930 consecutive patients with GERD were referred for endoscopy from Trikala prefecture, had an entry endoscopy and a follow-up if needed. Before endoscopic evaluation, all patients and their first-degree relatives completed Reflux Symptom Questionnaire. Patients were followed up for 4 years with Reflux Symptom Questionnaire every 6 months.
A total of 258 (62.9%) patients with positive and 724 (47.6%) with negative family history of GERD had esophagitis (P<0.0001). Seventy-six (74.5%) patients with more than 1 family member with GERD had esophagitis (P<0.0001). During follow-up endoscopic picture was aggravated in 101 (25%) patients with positive and 46 (3%) with negative family history. A total of 359 (24%) of GERD patients with negative and 24 (10%) with positive family history managed to stop proton pump inhibitors during follow-up (P<0.0001). In logistic regression analysis: age, male sex, presence of hiatal hernia, family history of GERD, tranquilizer use, frequency, and duration of reflux symptoms were independently associated with presence of esophagitis.
Although we cannot overlook the importance of confounding factors such as body weight and/or psychological factors, we found that endoscopic picture is more severe among GERD patients with at least 1 first-degree relative with GERD. During follow-up, patients with negative family history had more chances to wean off proton pump inhibitors after life-style modifications.
Journal of clinical gastroenterology 12/2011; 46(3):201-8. · 2.21 Impact Factor
-
Panagiota Mavrogianni,
George Alexandrakis,
Christina Stefanaki,
Maria Hadzivassiliou,
Andreas Karameris,
Aggeliki Mela,
George Bethimoutis, Periklis Apostolopoulos,
Panagiotis Tsibouris,
Andreas Katsambas,
Nikolaos Kalantzis
[show abstract]
[hide abstract]
ABSTRACT: Given that anorectal human papillomavirus (HPV) infection has been related to anal intraepithelial neoplasia (AIN) and rectal cancer, we conducted this study to evaluate the role of cytology of anal smears in the diagnosis of intraanal disease and related AIN and to correlate it to HPV genotypes.
A total of 72 patients (58 males and 14 females) with perianal warts underwent anoscopy with biopsies and anal cytologic examination. Cytology was carried out for the identification of any dysplasia according to the Bethesda system. All specimens were examined with polymerase chain reaction (PCR) for HPV DNA identification. Exclusion criteria included immunosuppression and high-grade squamous intraepitheliel lesion (HGSIL) or SCC in anal specimens.
Seven patients were excluded from the study. Intraanal warts were detected with anoscopy in 57 out of 65 patients, whereas histology showed HPV infection in 56 out of 65 patients and cytology was positive in 52 out of 65 low-grade squamous intraepitheliel lesion (LGSIL) patients. In 43 out of 52 positive patients, simple HPV infection was detected whereas in 9 out of 52 positive patients AIN I. HPV DNA was detected in 51 out of 65 patients, whereas 3 specimens were characterized as invalids. In the majority, HPV 6 could be identified (39/48, 81%), whereas HPV 16 was detected in 4 patients (4/48, 8.3%). One fourth of the positive patients had been infected with more than 1 HPV types (13/48, 27%). Cytology presented a sensitivity 87.5% and specificity 67% in comparison with the histology.
Cytology is highly sensitive in the diagnosis of intraanal warts comparable with histopathology. The combination of the 3 examinations (anoscopy, cytology, and PCR HPV typing) improves diagnostic accuracy and offers a global picture of the anorectal HPV disease.
Journal of clinical gastroenterology 10/2010; 45(4):e39-43. · 2.21 Impact Factor
-
Journal of clinical gastroenterology 03/2009; 43(6):599-600. · 2.21 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The role of capsule endoscopy (CE) in the diagnosis of active mild-to-moderate GI bleeding (GIB) immediately after a negative EGD and ileocolonoscopy has not been prospectively evaluated.
To estimate the diagnostic yield and clinical significance of CE in patients with acute, obscure, overt, mild-to-moderate GIB.
A single-center prospective study.
During a 3-year period, 573 patients admitted to the hospital with acute mild-to-moderate GIB were included in this study. Among them, 37 patients (6.5%) with negative endoscopic findings, after urgent upper- and lower-GI endoscopies, underwent CE within the first 48 hours to identify the source of bleeding.
CE revealed active bleeding in 34 patients and a diagnostic yield of 91.9%, including angiodysplasias in 18 patients, ulcers in 3 patients, and tumors in 2 patients. In the remaining 11 patients (32%), CE revealed the site of bleeding: distal duodenum in 1 case (9%), jejunum in 6 cases (54%), ileum in 2 cases (18%), and cecum in 2 cases (18%). From the 37 bleeders, 16 were managed conservatively, 14 endoscopically, and 7 surgically. During a 12-month follow-up period, bleeding recurrence was observed in 5 of 32 (15.6%).
This study had a limited number of patients.
CE appeared to have a high diagnostic yield in patients with acute, mild-to-moderate, active hemorrhage of obscure origin when performed in the hospital after a negative standard endoscopic evaluation and has important clinical value in guiding medical management.
Gastrointestinal Endoscopy 01/2008; 66(6):1174-81. · 4.88 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Capsule endoscopy is an easy and painless procedure permitting visualization of the entire small-bowel during its normal peristalsis. However, important problems exist concerning capsule retention in patients at risk of small bowel obstruction. The present report describes a young patient who had recurrent episodes of overt gastrointestinal bleeding of obscure origin, 18 years after small bowel resection in infancy for ileal atresia. Capsule endoscopy was performed, resulting in capsule retention in the distal small bowel. However, this event contributed to patient management by clearly identifying the site of obstruction and can be used to guide surgical intervention, where an anastomotic ulcer is identified.
World Journal of Gastroenterology 03/2007; 13(8):1289-91. · 2.47 Impact Factor
-
Gastrointestinal Endoscopy 02/2005; 61(1):187-8. · 4.88 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Natural killer (NK) cells represent a first line of defence against a developing cancer; however, their exact role in colorectal cancer remains undetermined. The aim of the present study was to evaluate the expression of CD16 and CD57 [immunohistochemical markers of natural NK cells] in colorectal adenocarcinoma.
Presence of NK cells was investigated in 82 colorectal adenocarcinomas. Immunohistochemical analysis was performed, using 2 monoclonal antibodies (anti-Fc Gamma Receptor II, CD16 and an equivalent to Leu-7, specific for CD-57). The number of immunopositive cells (%) was evaluated by image analysis. The cases were characterized according to: patient gender and age, tumor location, size, grade, bowel wall invasion, lymph node metastases and Dukes' stage.
NK cells were detected in 79/82 cases at the primary tumor site, 27/33 metastatic lymph nodes and 3/4 hepatic metastases; they were detected in levels similar to those reported in the literature, but their presence was not correlated to the clinical or pathological characteristics of the series, except for a negative association with the patients' age (p = 0.031).
Our data do not support an association of NK cell tissue presence with clinical or pathological variables of colorectal adenocarcinoma, except for a negative association with the patients' age; this might possibly be attributed to decreased adhesion molecule expression in older ages.
BMC Gastroenterology 10/2004; 4:20. · 2.42 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate changes in H pylori infection prevalence in Greece during a ten-year period, and to examine its antigenic profile.
Three groups of patients were studied. Group O-87: Banked serum samples of 200 consecutive adult outpatients, from the Hepato-Gastroenterology clinic of a teaching hospital at Athens, collected in 1987. Group O-97: Serum samples of 201 similarly selected outpatients from the same Unit, collected in 1997. Group BD-97: Serum samples of 120 consecutive blood donors from the same hospital, collected in 1997. H pylori IgG antibody seroprevalence was studied by a quantitative ELISA. Antigenic profile was studied by western-blot IgG assay, in 62 IgG positive patients of O-97 and BD-97. Results were analyzed by conventional statistics and multivariate regression analysis.
The H pylori seroprevalence increased with age in the three tested groups. In O-97, seroprevalence did not differ from that, in BD-97. On the contrary, there was a significant decrease in seropositivity between O-87 and O-97 (59.5% vs 49.2%, p = 0.039). Multiple regression analysis showed that age over 35 years (OR:3.45, 95% CI:1.59-7.49, p = 0.002) and year of patients' selection - that is 1987 or 1997 - (OR:1.73, 95% CI:1.14-2.65 for 1987, p = 0.010), were independent risk factors of H pylori infection. The seroprevalence of CagA+ and VacA+ strains was 77.4% and 58.5%, respectively, and type I(CagA+/VacA+) strains were significantly more common than type II(CagA-/VacA-) strains (59.7% vs 22.6%, p < 0.001).
During a ten-year period, we found a significant decrease of H pylori infection in Greece and our data support the birth cohort phenomenon as an explanation for the age-dependent increase of H pylori infection. The prevalence of CagA and/or VacA positive strains is relatively high, in a country with low incidence of gastric cancer.
BMC Gastroenterology 05/2002; 2:11. · 2.42 Impact Factor
