James C Mitchell

University of Oxford, Oxford, England, United Kingdom

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Publications (3)35.49 Total impact

  • Jonathan Malo · James C Mitchell · Andrew J Turberfield ·
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    ABSTRACT: We describe the three-layer logpile (3LL), a two-dimensional DNA array which self-assembles from four synthetic oligonucleotides via a four-armed Holliday junction motif. It consists of three layers of helices, each running at 60 degrees to the others. DNA arrays can be used as periodic templates to create, for example, synthetic protein crystals: this array is designed to maximize structural order by ensuring that helices run continuously, without bending, through the structure. UV absorbance measurements show a rate-dependent hysteresis associated with the assembly of the 3LL. Negative-stain transmission electron microscopy (TEM) of 3LL samples shows that the arrays form extensive sheets (approximately microm(2)) and a process of iterative correlation mapping and averaging of small subsets of digitized TEM micrographs yields an averaged projection image that is consistent with a computer-generated model of the crystal.
    Journal of the American Chemical Society 10/2009; 131(38):13574-5. DOI:10.1021/ja9042593 · 12.11 Impact Factor
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    ABSTRACT: Weben mit DNA: Mit einem DNA-bindenden Protein lässt sich die Struktur eines selbstorganisierten 2D-Kristalls steuern. Ohne das Protein hybridisieren vier Oligonucleotide zu miteinander verwobenen Doppelhelices mit p3-Symmetrie (siehe Bild). Zugabe des Proteins RuvA während der Selbstorganisation ändert die Symmetrie und Verknüpfung, und es resultiert ein DNA-Protein-Kristall mit annähernd quadratischer Elementarzelle.
    Angewandte Chemie International Edition 05/2005; 44(20):3057-61. DOI:10.1002/anie.200463027 · 11.26 Impact Factor
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    ABSTRACT: A system of DNA "tiles" that is designed to assemble to form two-dimensional arrays is observed to form narrow ribbons several micrometers in length. The uniform width of the ribbons and lack of frayed edges lead us to propose that they are arrays that have curled and closed on themselves to form tubes. This proposal is confirmed by the observation of tubes with helical order.
    Journal of the American Chemical Society 01/2005; 126(50):16342-3. DOI:10.1021/ja043890h · 12.11 Impact Factor