Fabio Camacho-Alonso

University of Murcia, Murcia, Murcia, Spain

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Publications (89)148.92 Total impact

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    ABSTRACT: Abstract Background. In recent years, periodontal disease has been related to a large number of systemic disorders. Objective. To study the possible relationship between periodontal disease and high levels of D-dimer in a group of patients with venous thromboembolic disease. Materials and methods. A study was made of 142 patients diagnosed with unprovoked venous thromboembolic disease. All patients underwent oral examination consecutively and randomly. Finally, two groups were obtained: (a) patients with periodontal disease (n = 71); and (b) patients without periodontal disease (n = 71). All patients were subjected to periodontal study, with evaluation of the number of teeth, bleeding index, gingival index, simplified oral hygiene index, community periodontal index of treatment needs, clinical attachment level, probe depth, number of pockets ≥4 mm, number of pockets ≥6 mm. The D-dimer values were evaluated in both groups. Results. D-dimer values were higher in the study group than the control group, with statistically significant differences (p = 0.010). Conclusions. A relationship is observed between the presence of periodontal disease and high D-dimer levels. Patients with venous thromboembolic disease and periodontal disease could have more risk of a new thromboembolism episode.
    Acta Odontologica Scandinavica 11/2014; · 1.31 Impact Factor
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    ABSTRACT: A study is made of DNA damage and apoptosis in a group of patients with oral leukoplakia (OL) with mild dysplasia.
    International journal of dermatology 06/2014; · 1.23 Impact Factor
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    ABSTRACT: The aim of this in vitro study was to investigate the impact fracture resistance of anterior teeth that had been fractured and restored with modified partial-coverage ceramics using the edge-up technique. A total of 178 extracted human mandibular central and lateral incisors were included in this study. The incisors were randomly divided into two groups. Group 1 (n = 89) teeth were cleaved and restored with modified partial-coverage ceramics using the edge-up technique; Group 2 (n = 89) consisted of intact teeth that served as control. Impact strength was tested in a modified impact testing machine (pendulum type), and the severity of crown fracture after fracture resistance test was registered. Impact strength was insignificantly lower in restored teeth (median 5.39 and range 0.81-14.12 kJ m(-2) ) than in intact teeth (median 5.45 and range 0.31-16.47 kJ m(-2) ), although no statistically significant differences were observed. Regarding the severity of crown fracture, restored teeth showed a lower rate of severe fractures (20.22%) compared to intact teeth (25.84%), but no statistically significant differences were observed. Restoration of cleaved mandibular incisors with modified partial-coverage ceramics using the edge-up technique shows fracture resistance and severity of crown fracture following traumatism similar to intact teeth. In this way, this technique can be used successfully for the treatment of fractured anterior teeth.
    Dental Traumatology 02/2014; · 1.21 Impact Factor
  • Pia López-Jornet, Fabio Camacho-Alonso, Mariano Sánchez-Siles
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    ABSTRACT: Purpose: The main aim of this study was to evaluate the patients with oral lichen planus (OLP) and dental implants. Material and Methods: Three groups of 16 patients took part in the study. Group I patients had received dental implants and been diagnosed with OLP; Group II had not received implants but were diagnosed with OLP; Group III had implants but not OLP. Clinical observations and OLP symptoms were registered in each case. Periodontal pocket depth, implant mobility, bleeding upon probing, erythema, pain, and radiolucency around implants were measured. Patient quality of life was evaluated using OHIP 14. Results: Peri-implant mucositis and peri-implantitis were detected in 17.86% and 25% of the OLP-implant group, while the control group with implants showed 18% and 16%. The implant survival rate in patients treated for OLP did not appear to differ from the survival rate among the general population. Quality of life was better among patients with implants and without OLP (p = .001). Conclusions: The results of the present study suggest that implants do not influence manifestations of OLP. OLP is not a risk factor for peri-implantitis.
    Clinical Implant Dentistry and Related Research 02/2014; 16(1):107-15. · 3.82 Impact Factor
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    ABSTRACT: Abstract Objective. To evaluate the effects of resveratrol and irradiation on oral squamous cell carcinoma (OSCC). Materials and methods. Resveratrol was administered at doses of 5, 10, 25, 50 and 100 µM to PE/CA-PJ15 (OSCC) cultures irradiated with different doses (1, 2.5 and 5 Gy). Effects upon cell viability, apoptosis and migration were evaluated after 24, 48 and 72 h incubation. Results. After 72 h of incubation, the 100 µM dose of resveratrol induced the greatest decrease in cell viability at all irradiation doses. After 24, 48 and 72 h of incubation, 100 µM of resveratrol induced the greatest cell apoptosis at all irradiation doses. The greatest alterations in the distribution of the G0-G1, G2-M and S phases of the cell cycle were recorded with 50 and 100 µM of resveratrol; after 24, 48 and 72 h of incubation, both these doses resulted in an increase in the S phase, at the expense of the G0-G1 and G2-M phases. Conclusions. Resveratrol increases cytotoxic activity in the PE/CA-PJ15 cell line and reduces cell migration capacity, while the combination of resveratrol and irradiation exerts a synergic effect.
    Acta odontologica Scandinavica 01/2014; · 1.41 Impact Factor
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    ABSTRACT: A study was undertaken to determine the general level of knowledge, attitudes and practices in clinical dental care of pregnant women. This was a transversal descriptive study in the form of a structured and anonymous survey completed by dentists in Murcia (southeast Spain). The questionnaire was sent by e-mail and investigated dentists' knowledge of and attitudes toward different dental procedures applying to pregnant women. The response rate to the questionnaire was 60%. The vast majority of dentists (81.3%) agreed that oral care should be part of general healthcare for pregnant women. However, discrepancies were found in knowledge and attitudes to practice.
    The New York state dental journal 01/2014; 80(1):38-41.
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    ABSTRACT: The aim was to investigate the accuracy of quantitative computed tomography bone mineral density (BMD) measurements in mandibles, comparing measured BMD with calibrated BMD. Seventy mandibles from adult cadavers were used. Twenty tomographic cuts were made in each mandible. In each tomographic cut, a region of interest was located, and the bone density was measured in Hounsfield unit (HU). A polymethyl methacrylate phantom containing four inserts of different predetermined densities (hydroxyapatite 100, 200, 500, and 700 mg/cm(3) ) was used to calculate calibrated bone density. Correlation between measured and calibrated bone densities was calculated. Mean total correlation between measured and calibrated BMD in the 20 sagittal tomography cuts showed almost perfect positive correlation (r = 0.998, p < .001). However, when average BMD measurements in HU were compared, the measured total BMD (in the 20 sagittal tomography cuts studied) was 54.99 ± 421.59, whereas the total calibrated BMD was 49.28 ± 364.95, with statistically significant difference (p = .001). There are discrepancies between measured and calibrated BMD; in this sense, a calibrated bone phantom with a predetermined mineral density should be used to determine the exact BMD before dental implants surgery.
    Clinical Implant Dentistry and Related Research 11/2013; · 3.82 Impact Factor
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    ABSTRACT: Titanium is the most widely used metal in implant dentistry. In spite of its biocompatibility, when it is released into the oral environment, it can have local negative biological effects. The aims of this study were to detect the concentration of metal ions in patients with dental implants, to evaluate whether or not their release might be influenced by the presence of other metals, and to assay whether these ions might provoke genotoxic damage in oral mucosa cells. One hundred five patients with a total of 180 dental implants were included. The sample was divided into seven groups (n = 15 per group). Group 1 consisted of patients with metal-porcelain fixed crowns on dental implants; Group 2, patients with metal-porcelain fixed crowns on teeth; Group 3, patients with dental amalgams; Group 4, patients with metal-porcelain fixed crowns on dental implants and metal-porcelain fixed crowns on teeth; Group 5, patients with metal-porcelain fixed crowns on dental implants and dental amalgams; and Group 6, patients with metal-porcelain fixed crowns on dental implants, metal-porcelain fixed crowns on teeth, and dental amalgams. Group 7 was the control group, without any dental treatment. The concentration of metal ions was detected using inductively coupled plasma mass spectrometry; genotoxicity was measured using the buccal micronucleus cytome assay protocol. Group 5 displayed the highest concentration of metal ions in parts per billion (Ti, Co, Ni, Zn, Pd, Sn, and Pb). Group 6 was characterized by the highest presence of Hg. No signs of genotoxic damage were found in any of the study groups. Patients with titanium dental implants combined with other metal restorations presented higher concentrations of metal ions, but no genotoxic damage was observed in oral mucosal epithelial cells.
    Clinical Implant Dentistry and Related Research 10/2013; · 3.82 Impact Factor
  • Pia Lopez-Jornet, Fabio Camacho-Alonso, Nieves Martos
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    ABSTRACT: A study was made of the association between recurrent aphthosis (RAS) and iron, ferritin, vitamin B12, and folic acid deficiency. A total of 186 adults were consecutively recruited: 92 subjects with RAS (28 males and 64 females, with a mean age of 41 years) and 94 controls (20 men and 74 women, with a mean age of 44 years), and subjected to clinical and hematological evaluation. The overall frequency of hematinic deficiencies was 14.14% in the RAS group versus 6.39% in the control group (P = 0.086). Patient age and a family history of aphthosis were associated to the presence of RAS. There is still no conclusive evidence relevant to the etiopathogenesis of RAS. Routine hematological screening and tests for serum iron, folic acid, and vitamin B12 deficiencies should be assessed in all patients with RAS.
    International journal of dermatology 07/2013; · 1.23 Impact Factor
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    ABSTRACT: Objective: The aim of this study was to compare the effects in terms of resistance to fracture of the mandibular condyle and femoral head following different doses of zoledronic acid in an animal model. Study design: A total of 80 adult male Sprague-Dawley rats were included in a prospective randomized study. The animals were randomly divided into four groups of 20 rats each. Group 1 (control) received sterile saline solution, while groups 2, 3 and 4 received a accumulated dose of 0.2 mg, 0.4 mg and 0.6 mg of zoledronic acid, respectively. The animals were sacrificed 28 days after the last dose, and the right hemimandible and the right femur were removed. The fracture strength was measured (in Newtons) with a universal test machine using a 1 kN load connected to a metal rod with one end angled at 30 degrees. The cross-head speed was 1 mm/min. Later, the specimens were observed under a scanning electron microscope with backscattered electron imaging (SEM-BSE). At last, chemical analysis and elemental mapping of the mineral bone composition were generated using a microanalytical system based on energy-dispersive and X-ray spectrometry (EDX). Results: A total of 160 fracture tests were performed. The fracture resistance increased in mandible and femur with a higher accumulated dose of zoledronic acid. Statistically significant differences were recorded versus the controls with all the studies groups. The chemical analysis in mandible showed a significantly increased of calcium and phosphorous to compare the control with all of the study groups; however, in femur no statistically significant differences between the four study groups were observed. Conclusions: The administration of bisphosphonates increases the fracture resistance in mandible and femur.
    Medicina oral, patologia oral y cirugia bucal 03/2013; · 1.10 Impact Factor
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    ABSTRACT: Keratocystic odontogenic tumor is an intraosseous benign tumor of epithelial origin that can appear as a unicystic or multicystic lesion. It is characterized most significantly by its high recurrence rate. This is why surgical removal is the chosen therapeutic approach in most cases. However, while surgery may be the safest technique to prevent recidivism, it may also result in numerous complications with large tumors. A number of authors have suggested a more conservative treatment for large keratocystic odontogenic tumors. The case examined in this article is that of a 48-year-old male patient who presented with a five-month swelling on the left side of his face, located in the mandibular area.
    The New York state dental journal 03/2013; 79(2):44-7.
  • Pia López-Jornet, Fabio Camacho-Alonso, Mariano Sanchez-Siles
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    ABSTRACT: Our aim was to analyse the amount of anxiety and fear felt before, immediately after, and one week after, dental extraction. We studied 70 patients (35 men and 35 women (mean (SD) age 43 (±10) years), who were listed for dental extraction under local anaesthesia in a private clinic that specialised in oral surgery. Patients were evaluated on 3 consecutive occasions: immediately preoperatively, immediately postoperatively, and 7 days later. Each patient's anxiety was measured using Spielberger's State-Trait Anxiety Inventory (Spanish version), the Modified Corah Dental Anxiety Scale (MDAS) and the Dental Fear Survey. There were significant differences in the STAI-Trait scale between before and 7 days after extraction (p=0.04), and in the MDAS between before and immediately after extraction (p=0.02), and between immediately after and 7 days after extraction (p=<0.001). The DFS also differed between before and immediately after extraction (p=0.002), and between immediately and 7 days after extraction (p<0.001). Dental anxiety immediately after tooth extraction may be influenced by operative techniques (type of anaesthesia, duration of operation, or position of tooth extracted), but anxiety at 7 days after extraction is not.
    British Journal of Oral and Maxillofacial Surgery 01/2013; · 1.13 Impact Factor
  • Article: Correction.
    Oral Diseases 01/2013; 19(1):109-110. · 2.40 Impact Factor
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    P López-Jornet, F Camacho-Alonso
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    ABSTRACT: Objective: The aim of this study was to compare conventional surgery with carbon dioxide (CO2) laser in patients with oral leukoplakia, and to evaluate the postoperative pain and swelling. Study design: A total of 48 patients (27 males and 21 females) with a mean age of 53.7 ± 11.7 years and diagnosed with oral leukoplakia were randomly assigned to receive treatment either with conventional surgery using a cold knife or with a CO2 laser technique. A visual analog scale (VAS) was used to score pain and swelling at different postoperative time points. Results: Pain and swelling reported by the patients was greater with the conventional cold knife than with the CO2 laser, statistically significant differences for pain and swelling were observed between the two techniques during the first three days after surgery. Followed by a gradual decrease over one week. In neither group was granuloma formation observed, and none of the patients showed malignant transformation during the period of follow-up. Conclusions: The CO2 laser causes only minimal pain and swelling, thus suggesting that it may be an alternative method to conventional surgery in treating patients with oral leukoplakia.
    Medicina oral, patologia oral y cirugia bucal 12/2012; · 1.10 Impact Factor
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    ABSTRACT: Tissue repair is a complex process, which may be favoured or inhibited by different factors. Potassium apigenin (AP) and other flavonoids present in verbena extract (PLX®) possess powerful anti-inflammatory properties. The aim of this study was to evaluate the effects of topical treatment with AP and PLX gels on wounds inflicted on SKH-1/CRL mice. Forty-eight SKH-1 mice were used (4 groups with 12 animals each), which were subjected to wound excision with a round scalpel, 4 mm in diameter, on the dorsal skin. Animals were divided into four groups: Group I received topical applications of apigenin gel; Group II received PLX gel; Group III received vehicle gel; Group IV acted as control. Wound contraction, re-epithelialisation, inflammation and neovascularisation (by means of immunohistochemical staining with anti-laminin) were recorded at study periods established at 2, 7 and 14 days. Re-epithelialisation was faster in Groups I and II at 7 days (56·25% grade 3 and 43·75% grade 4) compared with the other groups. The degree of inflammation showed improvement with a tendency towards statistical significance in Groups I and II at 2 and 7 days. Anti-laminin staining was more intense in the group treated with PLX at the 2- and 7-day periods. Topical treatment with PLX gel improved the degree of re-epithelialisation and inflammation and favoured neo-vascularisation of the wounds at 2 and 7 days following surgery.
    International Wound Journal 11/2012; · 2.02 Impact Factor
  • F Camacho-Alonso, P López-Jornet, Mr Tudela-Mulero
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    ABSTRACT: OBJECTIVE: An in vitro study was carried out to evaluate the effect of curcumin, lycopene, and irradiation upon oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Curcumin and lycopene were administrated at doses of 3, 4.25, 5.50, and 6.75 μM in PE/CA-PJ15 OSCC cultures irradiated with different doses (1, 2.5, and 5 Gy), followed by evaluation of the effects upon cell viability, apoptosis, and migration after 24, 48, and 72 h of incubation. RESULTS: The application of curcumin or lycopene to the tumor cells during 24, 48, and 72 h without irradiation exerted an inhibitor effect upon cell viability and increased cell apoptosis. The maximum reduction in cell viability and the peak apoptotic effect was recorded with the 5.50 and 6.75 μM doses, for both curcumin and lycopene. Likewise, curcumin and lycopene exerted a synergic effect upon both variables on applying irradiation. Lastly, the 5.50 and 6.75 μM drug doses, together with 5 Gy of irradiation, yielded the greatest decrease in cell migration capacity with both curcumin and lycopene. CONCLUSIONS: Curcumin and lycopene increase cytotoxic activity in the PE/CA-PJ15 cell line and reduce cell migration capacity, while the combination of curcumin or lycopene with irradiation exerts a synergic effect.
    Oral Diseases 09/2012; · 2.40 Impact Factor
  • Pia López-Jornet, Fabio Camacho-Alonso, Diana Molino-Pagan
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    ABSTRACT: OBJECTIVES: The aim of this study was to evaluate the efficacy of aloe vera (AV) applied in combination with a tongue protector, comparing this with a placebo. METHODS: A total of 75 patients with burning mouth syndrome (BMS) were divided into three groups randomly: Group I (tongue protector three times a day), Group II (tongue protector and 0.5 ml AV at 70% three times a day) and Group III (tongue protector and 0.5 ml placebo three times a day). Symptoms were evaluated by visual analogue scale (VAS), while patient psychological profiles were assessed using the Hospital Anxiety-Depression scale and their quality of life using the Oral Health Impact Profile 49 (OHIP-49). Treatment continued for 3 months. RESULTS: Visual analogue scale pain values improved for all three study groups but without statistically significant differences between the groups (P = 0.210). Regarding quality of life, no significant differences were found between groups with the exception of the OHIP-49 score for handicap. The overall clinical improvement was greater for Group II, with a difference almost reaching significance. CONCLUSIONS: The concomitant prescription of tongue protector and AV is effective for treating patients with BMS.
    Journal of Oral Pathology and Medicine 09/2012; · 1.87 Impact Factor
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    ABSTRACT: OBJECTIVES: Oral cavity and pharyngeal cancer has been well recognized as a public health problem. Prognosis for oral cancer is substantially improved when diagnosed early. A study was made of the impact of different educational strategies upon knowledge of oral cancer, evaluating the intervention after 4 weeks. METHODS: Ninety subjects over 40 years of age were consecutively included and randomized to three different groups (video, leaflet, verbal information). All subjects completed a 20-item pre-test on their knowledge of the risk factors, signs, and symptoms of oral cancer, and posteriorly received the video, leaflet, or verbal information, with evaluation of the results after 4 weeks. RESULTS: In relation to the items correctly answered at baseline, all subjects showed an increase in knowledge score after 4 weeks: verbal information 4.77 ± 3.31, leaflets 4.53 ± 3.44, and video 3.67 ± 3.31 - no statistically significant differences being observed for any of the three strategies. CONCLUSION: The oral cancer knowledge score increased following the educational intervention, which proved successful over the short term. Copyright © 2012 John Wiley & Sons, Ltd.
    Psycho-Oncology 09/2012; · 4.04 Impact Factor
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    ABSTRACT: Background: Oral lichen planus (OLP) is a chronic inflammatory disorder. Recently, a case-control study found that lichen planus was associated with dyslipidemia in a large series of patients. However, no data were presented about lipid values in patients and controls. Objectives: The aim of this study was to investigate the hypothetical association between OLP and dyslipidemia. Materials and Methods: The study included a total of 400 patients (200 with OLP and 200 controls with other oral diseases) and investigated the prevalence of dyslipidemia. The variables analyzed were age, sex, tobacco and alcohol consumption, clinical form of OLP and lipid profiles. Results: A 54% prevalence of dyslipidemia was found (58% among the OLP group and 50% in the control group). Statistically significant differences in high-density lipoprotein were found between OLP patients and the control group (p = 0.003). A logistic regression model for presence/absence of cardiovascular risk (Castelli's atherogenic index of ≥5.1 for men and ≥4.5 for women) found statistically significant differences for sex and tobacco consumption. Conclusions: The study found a higher atherogenic index amongst OLP patients.
    American Journal of Clinical Dermatology 08/2012; 13(6):399-404. · 2.52 Impact Factor
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    ABSTRACT: Oral Diseases (2012) 18, 786-792 Objective:  In the last two decades, the use of mobile phones has increased enormously all over the world. The controversy regarding whether radiofrequency (RF) fields exert effects upon biological systems is a concern for the general population. An evaluation is made of DNA damage and cytokinetic defects, proliferative potential, and cell death because of RF radiation emitted by mobile phones in healthy young users. Study design:  This cohort study was carried out in 50 Caucasian mobile phone users. We collected two cell samples from each subject (a total of 100 cell samples), corresponding to the right and left cheek mucosa, respectively. Case histories and personal information were assessed, including age, gender, body height and weight, history of cancer, smoking and alcohol consumption, exposure to chemical carcinogens or radiation, and dietary habits. Sampling comprised cell collection from both cheeks with a cytobrush, centrifugation, slide preparation, fixation, and staining, followed by fluorescent microscopic analysis. A total of 2000 exfoliated cells were screened for nuclear abnormalities, especially micronucleus. Results:  No statistically significant changes were recorded in relation to age, gender, body mass index, or smoking status. A comparison of the results vs the control area according to the side of the face on which the mobile phone was placed, and in relation to the duration of exposure (years) to mobile phone radiation in the total 100 samples, yielded no significant differences. Conclusions:  No genotoxic effects because of RF exposure were observed in relation to any of the study parameters.
    Oral Diseases 05/2012; 18(8):786-92. · 2.40 Impact Factor