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ABSTRACT: To explore the effects of Candesartan cilexetil on the rats exposed to silica.
Ninety-six wistar rats were randomly divided into model-group, intervention-group and control-group (32 rats a group). The intervention-group, model-group and control group were orally exposed to Candesartan cilexeti (10 mg/kg) and normal solution for a week, respectively. Then the model and intervention groups were exposed to silica by intratracheal infusion of silica dust suspension (50 mg/ml), the control group was exposed to 0.5 ml normal solution for 2 days. On the 3rd, 7th, 14th and 28th days after exposure to silica, 8 rats of each group were sacrificed, respectively. The samples of lung tissues were collected. The lung/body coefficients were detected. The pathological examinations were performed by HE and Masson staining. The levels of ACE in the lung tissues were observed by immunochemistry staining. The levels of TGF-β1 and Ang II in the BALF were examined by ELISA.
On the 3rd, 7th, 14th and 28th days after exposure, the levels of alveolitis and pulmonary fibrosis in the intervention group were significantly alleviated as compared with model group, and the lung/body coefficients in the intervention group, which were significantly lower than those in model group respectively (P < 0.01). As compared with control group, the levels of TGF-β1 and Ang II of the BALF in the model and intervention groups significantly enhanced (P < 0.01). As compared with model group, the levels of TGF-β1 and Ang II of the BALF in the intervention group significantly decreased (P < 0.01). As compared with control group, the levels of ACE of the lung tissues in the model and intervention groups significantly increased (P < 0.01). But the level of ACE of the lung tissues in the intervention group was significantly lower than that in the model group (P < 0.01).
The early Candesartan cilexetil intervention could significantly decrease the levels of alveolitis and lung fibrosis, declined the levels of TGF-β(1) and Ang II of BALF and downregulated the expression level of ACE in lung tissues in rats exposed to silica.
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 04/2012; 30(4):250-4.
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ABSTRACT: To explore the effects of high-dose N-acetylcysteine on the lung tissues of rats exposed to silica.
Ninety-six Wistar rats were randomly divided into model group, intervention group and control group (32 rats for each group). The rats of model group and intervention group were exposed to silica by intratracheal infusion of silica dust suspension. The rats in the intervention group were orally given high dose N-acetylcysteine. In 3, 7, 14, 28 days after exposure, eight rats in each group were sacrificed, respectively and the lung samples were collected. The pathological changes of lung were evaluated by HE and Masson staining methods. The levels of TNF-alpha and IL-8 in the BALF were detected by ELISA.
Compared with the control group, the alveolitis and pulmonary fibrosis in the intervention group were significantly reduced. In 3, 7, 14, 28 days after exposure, the lung/body coefficients in the intervention group were 9.30 +/- 0.78, 6.29 +/- 0.74, 7.63 +/- 0.88, 6.06 +/- 1.16 respectively, which were significantly lower than those (13.84 +/- 1.61, 9.23 +/- 0.87, 11.23 +/- 1.25, 9.56 +/- 0.76, P < 0.01 ) in the model group (P < 0.01). At the different time points, the levels of TNF-alpha and IL-8 in the BALF in the intervention group were significantly higher than those in the control group (P < 0.01), but were significantly lower than those in the model group (P < 0.01).
The intervention with high dose N-acetylcysteine can significantly reduce the alveolitis and the TNF-alpha and IL-8 levels in the BALF, therefore, inhibit and delay the development of pulmonary fibrosis of rats exposed to silicon dioxide.
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 07/2011; 29(7):510-3.
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ABSTRACT: To determine the reference value of plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) in subjects without heart diseases.
The plasma concentration of NT-proBNP was measured with ELISA method in 300 adults excluded heart disease through various examinations including electrocardiography, echocardiography, X-ray and coronary artery angiography. The plasma NT-proBNP concentration was compared between age-groups 30-39, 40-49, 50-59, 60-69 and > or = 70 years old, between male and female in the same age-group and between subjects with and without hypertension, diabetes and obesity. A multiple linear regression analysis was used to detect factors influencing NT-proBNP among age, sex, body mass index, blood pressure, heart rate, serum creatinine, hypertension, diabetes mellitus, use of angiotensin-converting-enzyme inhibitors, Ca(2+)-antagonist, and beta-blocker.
The plasma NT-proBNP concentration increased in proportion to aging in male subjects more than 60 years old (P < 0.05), remained unchanged in males less than 60 years old and females (P > 0.05). Plasma NT-proBNP concentration was significantly higher in female (170-660 pmol/L) than in male (160-470 pmol/L) in subjects less than 60 years old (P < 0.05) and significantly lower in female (180-560 pmol/L) than in male (180-760 pmol/L) in subjects more than 60 years old (P < 0.05). Multiple linear regression analysis demonstrated that age was the only independent predictor for plasma NT-proBNP in these subjects (P < 0.01).
The plasma concentration of NT-proBNP in subjects without heart diseases was different between male and female, and was increasing with age in male subjects more than 60 years old.
Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] 09/2009; 37(9):804-8.
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ABSTRACT: A pot experiment was conducted to study the effects of two controlled-release fertilizers CRFA (4% resin-coated, N: P2O5: K2O = 14: 14: 14) and CRFB (4% resin-coated, N: P2O5: K2O = 20: 8:10) on the nutrient uptake and growth of Chrysanthemum morifolium, with common compound fertilizer CCF (N: P2O5: K2O = 15: 15: 15) as the control. Six treatments were installed, i. e., CCF1 (CCF, 6 g N x pot(-1)), CCF2 (CCF, 3 g N x pot(-1)), CRFA1 (CRFA, 6 g x pot(-1)), CRFA2 (CRFA, 3 g x pot(-1)), CRFB1 (CRFB, 6 g x pot(-1)), and CRFB (CRFB, 3 g x pot(-1)). On the 30th day of applying common compound fertilizer CCF1 and CCF2, soil available N, P and K contents were 163.29 and 145.26 mg x kg(-1), 180.39 and 163.13 mg x kg(-1), and 300.08 and 213.15 mg x kg(-1), respectively, and decreased rapidly since then. In treatments CRFA1, CRFB1, CRFA2, and CRFB, soil available N content increased slowly, and reached the peak on the 60th day after fertilizing, being 129.51, 138.65, 118.36, and 126.31 mg x kg(-1), respectively. Soil available P content had the same variation trend. Its maximum concentration was 169.54 and 133.46 mg x kg(-1) in treatments CRFA1 and CRFA2 on the 30th day after fertilizing, and 137.13 and 84.68 mg x kg(-1) in treatments CRFB1 and CRFB2 on the 60th day after fertilizing, and decreased slowly then. The agronomic traits such as leaf area, leaf area index, branch number, flowering rate, flower number, and flower diameter, etc., in treatments CRFA and CRFB were obviously better than those of the control, and CRFB was better than CRFA, suggesting that CRFB more matched the nutrient demand of C. morifolium. Under the conditions of present experiment, applying CRFB2 obtained the highest yield.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 07/2009; 20(7):1671-7.
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Bing-qi Wei,
Yue-jin Yang,
Jian Zhang,
Ke-fei Dou,
Yu-hui Zhang,
Xiao-hong Huang,
Lian-ming Kang, Chun-ling Zhang,
Qing Gu,
Xin Gao,
Yan-min Yang,
Yan Dai,
Li-tian Yu,
Hui-min Zhang,
Rong Lü
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ABSTRACT: To evaluate the predictive value of admission plasma amino-terminal pro-B-type natriuretic peptide (NT-proBNP) on in-hospital mortality in patients with decompensated heart failure.
Plasma NT-proBNP levels were measured in patients with decompensated heart failure within 24 hours after admission with ELISA method. The NT-proBNP levels were compared between survivals and dying patients in hospital. ROC analyses were performed to evaluate the predictive value of admission plasma NT-proBNP on in-hospital mortality and to identify the optimal NT-proBNP cut-point for predicting in-hospital mortality. A binary logistic regress analyses was used to evaluate if NT-proBNP was an independent predictor for in-hospital mortality.
A total of 804 patients with decompensated heart failure were enrolled in his study (293 valvular heart diseases, 219 ischemic cardiomyopathy, 141 dilated cardiomyopathy, 14 hypertrophic cardiomyopathy, 21 restrictive cardiomyopathy, 39 hypertensive heart disease, 41 chronic pulmonary heart disease and 36 adult congenital heart disease) and 96 patients were in class II, 450 in class III and 258 in cases IV according to NYHA Classification. During hospitalization, 64 deaths were recorded and the on admission plasma NT-proBNP levels of patients died during hospitalization were significantly higher than those of survivals [4321.1 (3063.8, 6606.5) pmol/L vs. 1921.6 (873.9, 3739.2) pmol/L, P<0.01]. Area under receiver operating characteristic curve (AUC) of NT-proBNP to predict in-hospital death was 0.772 (95% CI: 0.718 - 0.825, P<0.01), the optimal plasma NT-proBNP cut-point for predicting in-hospital mortality was 3500 pmol/L, with a sensitivity of 70.3%, a specificity of 72.0%, an accuracy of 71.9%, a positive predictive value of 17. 8% and a negative predictive value of 96.6%. Patients whose NT-proBNP levels were equal or more than 3500 pmol/L had a much higher in-hospital mortality (17.8%) compared with those with NT-proBNP levels of less than 3500 pmol/L (3.4%), P<0.01. Binary logistic regress analyses demonstrated that admission plasma NT-proBNP, pneumonia, heart rate and NYHA class were independent predictors for in-hospital mortality in patients with decompensated heart failure (P<0.05 or 0.01) and admission plasma NT-proBNP was the strongest predictor for in-hospital mortality.
Admission plasma NT-proBNP level was an independent predictor for in-hospital mortality in patients with decompensated heart failure. The optimal NT-proBNP cut-point for predicting in-hospital mortality was 3500 pmol/L in this patient cohort.
Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] 06/2009; 37(6):481-5.
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ABSTRACT: To observe the correlation of plasma amino-terminal pro-A-, B- and C-type natriuretic peptide (NT-proANP, NT-proBNP and NT-proCNP) levels with New York Heart Association (NYHA) functional class and echocardiographic derived parameters of cardiac function in heart failure patients.
Data of NYHA grade, echocardiographic derived parameters of cardiac function, plasma levels of NT-proANP, NT-proBNP and NT-proCNP (measured by enzyme immunoassay method) were obtained in 112 heart failure patients and 44 normal control subjects. The correlation analysis was made between NT-proANP, NT-proBNP, NT-proCNP and NYHA functional class, left atrium diameter (LAD), left ventricular end-diastolic diameter (LVEDD) and left ventricular ejection fraction (LVEF), respectively.
The plasma concentrations of NT-proANP, NT-proBNP and NT-proCNP in heart failure patients were significantly higher than in control group (all P<0.05). Correlation analysis revealed a strong correlation between NT-proANP and NT-proBNP (r = 0.790, P = 0.000) and a weak correlation between NT-proCNP and NT-proBNP (r = 0.278, P = 0.003) as well as between NT-proCNP and NT-proANP (r = 0.236, P = 0.012) in heart failure patients. Univariant analysis showed that NT-proANP and NT-proBNP were positively correlated to LAD, LVEDD and negatively correlated to LVEF (all P<0.05) while there was no significant correlation between NT-proCNP and echocardiographic derived parameters of cardiac function in heart failure patients. Multivariate stepwise regression analysis including age, gender, NYHA classification, LAD, LVEDD and LVEF revealed that NYHA classification, LVEF, LAD and age were independent predictors of NT-proANP; while NYHA classification, LVEF and age were independent predictors of NT-proBNP while there was no association among these factors and NT-proCNP.
In heart failure patients, the plasma concentration of NT-proANP, NT-proBNP and NT-proCNP were significantly increased and NT-proANP, NT-proBNP but not NT-proCNP were significantly correlated to NYHA classification and echocardiographic derived parameters of cardiac function.
Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] 06/2009; 37(6):486-90.
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ABSTRACT: To select the items from the Chinese menopause rating scale (CMRS) through pre-testing those people with menopausal syndromes.
293 people were surveyed in Guangzhou in 2005, among which 196 people with menopausal syndromes and others without. Psychometrics methods were employed to develop the scale. The item pools were all round. Methods used would include: focus group discussion and interviews, subjective evaluation method and Delphi method, to preliminarily screen the items. Data on scales measured from 196 cases with and 97 subjects without menopausal syndromes during the menopausal period, were collected. Again, seven statistical methods were employed to select the items.
The 40-items scale for menopausal syndrome was formed to include: a) three domains: somatic (18-items), psychological (14-items) and social (5-items); b) one general appraisal item; c) two lie-test items.
The Chinese menopausal syndrome scale we used seemed to possess good content validity, feasibility and intra-class reliability.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 11/2008; 29(10):985-8.
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ABSTRACT: To evaluate the feasibility, reliability, validity and responsiveness of a Chinese Menopause Rating Scale (CMRS).
Cross-sectional survey and convenience sampling were adopted. Participants: women with menopause syndrome and those in menopause but without menopause syndrome were recruited. All participants were asked to complete the CMRS, Kupperman Index, WHOQOL-BREF and MENQOL. The Self-control observation design was adopted when the responsiveness was evaluated. Patients were treated with TCM for weeks. MRSTCM was evaluated before and after the treatment.
(1) Feasibility: 3343 participants including 2320 patients and 1023 menopause women, were surveyed in 8 different settings. The recovery rate of CMRS was 100%, with a response rate as 99.7%. The completion of the CMRS took 10.30 minutes on average. (2)Reliability: Cronbach's alpha of CMRS, soma dimension, psychology dimension and community dimension of CMRS were 0.93, 0.87, 0.89 and 0.73 respectively, with the correlation coefficient of split half of the CMRS. Soma dimension, psychology dimension and community dimension were 0.92, 0.89, 0.86 and 0.73 respectively and the test-retest correlation coefficient of MRSTCM, the soma dimension, psychology dimension and community dimension were as 0.88, 0.91, 0.85 and 0.77 respectively. (3) Validity: CMRS was established on the basis of connotation of menopause syndrome, and a series of steps were adopted to modify the scale. CMRS was applicable for patients with menopause syndrome. CMRS seemed to have had good content-related validity. The result of exploratory factor analysis was accorded with the theory frame of CMRS by and large. The correlations between CMRS and KI, CMRS and WHOQOL-BREF, CMRS and MENQOL seemed good. The CMRS was able to discriminate between groups of people with or without menopausal syndrome and had good discriminative validity. (4) Responsibility: The CMRS was measured based on 174 patients with menopausal syndrome before and after the TCM therapy. Our result showed that the CMRS having the ability to measure the clinically important differences.
CMRS was suitable for outcome assessment of menopausal syndrome. This primary research proved that the CMRS had good feasibility, reliability, validity as well as responsiveness.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 10/2008; 29(9):882-6.
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ABSTRACT: A randomized single-blind trial was conducted to evaluate the efficacy of short-term abdominal acupuncture as a novel treatment for insomnia in Chinese women. Forty-four patients between the ages of 22 and 56 were randomly assigned to an acupuncture (n = 23) and a medication group (n = 21). The acupuncture group received abdominal acupuncture once a day for the first three days and once every three days for the remaining 11 days. In addition, every subject in acupuncture group also received a placebo pill once daily. Abdominal acupuncture was administered according to a standardized protocol involving four master and four adjunctive acupoints: Zhongwan (CV 12), Xiawan (CV 10), Guanyuan (CV 4), and Qihai (CV 6); bilateral Shangqu (KI 17), Huaroumen (ST 24), Xiafengshidian, and Qipang. Subjects in the medication group were treated with sham acupuncture at the same time as the acupuncture group and received estazolam once a day. The outcome measure was the Leeds Sleep Evaluation Questionnaire (LSEQ), administered before and after the trial. Subjects who received abdominal acupuncture lowered their LSEQ scores by an average of 26.32 points (95% CI: 37.34, 15.30). After controlling for potential confounding factors, the effect of abdominal acupuncture in relieving insomnia was still statistically significant. Results indicate that short-term abdominal acupuncture is more effective than pharmacological treatment for relieving insomnia in adult women and has few adverse effects.
Acupuncture & electro-therapeutics research 02/2008; 33(1-2):33-41. · 1.30 Impact Factor
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ABSTRACT: To prepare the PEGylated liposomes modified with cell penetrating peptides, which protect siRNA from nuclease degradation and deliver efficiently siRNA into cells to facilitate silencing of target gene.
The purity of R8-PEG-PE and pNP-PEG-PE was detected by HPLC; the quantity of R8, PEG-DPPE modified R8, and R8 attached to the out membrane surface of the liposomal siRNA by transfer from R8-PEG-DPPE micelles to the liposomes was tested by fluorescence; Size and size distribution of siRNA loaded liposomes with and without attached R8 were determined by Zetasizer 5000; A comparison of mediated siRNA transfection efficiency between R8-liposomes and lipofectamine 2000 was examined by individual inside cell fluorescence intensity; The growth inhibition of small cell lung carcinoma NCI-H446 cells treated with R8-liposomal hdm2-siRNA or lipofectamine 2000-hdm2-siRNA complex was tested by MTT assay.
The retention times of PEG-DPPE and R8-PEG-DPPE were 9.0 min and 7.8 min, respectively. Fluorescence scanning indicated that lipids composed of liposomes and siRNAs didn't interfere to the determination of R8 when it was attached to the liposomal siRNA. The cells treated with R8-liposomal hdm2-siRNA significantly enhanced the cellular uptake of hdm2-siRNA and facilitated the functions of hdm2-siRNA through silencing of target gene which, in turn, inhibited tumor cell growth, compared with lipofectamine 2000.
The R8 attached liposomes are shown to be powerful carriers for delivery siRNAs into cell to silence targeted gene.
Yao xue xue bao = Acta pharmaceutica Sinica 03/2006; 41(2):142-8.
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ABSTRACT: To introduce Menopause-Specific Quality of Life (MENQOL) into China, and to evaluate the quality of its Chinese Version.
MENQOL was translated into Chinese language and the reverse translation was done by several specialists. After retest study on 30 cases, validity, reliability and responsiveness were implemented in 409 cases with menopause syndrome in the multicenter study.
7 components were extracted in factor analysis, and the total cumulative contribution was 59.476%. By correlation analysis, 7 components were divided into four domains: vasomotor, psychosocial, physical and sexual items, same as in the English Version. The test-retest reliability of four domains were 0.806, 0.804, 0.941, 0.940 respectively. Cronbach alpha of four domains were 0.7258, 0.8234, 0.8475, 0.8641 respectively. There had been significant changes after treatment noticed.
The measurement properties of MENQOL-Chinese Version including effectueness, reliability were met with satisfaction and seemed to be adaptable to Chinese menopause women.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 02/2005; 26(1):47-50.
