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Long-Gen Xu,
Li-Ming Jin,
Xiao-Feng Zhu,
Qi-Zhe Song,
Xian-Fan Ding,
Shu Han,
You-Hua Zhu,
Yong Liu,
Hong-Wei Wang,
Jing Fu,
Yi-Rong Yang,
Feng Qiu,
Wan-Ling Peng,
Li-Gong Tang, Nian-Qing Lu
Transplantation 12/2008; 86(10):1480-1. · 4.00 Impact Factor
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Zhonghua nan ke xue = National journal of andrology 04/2008; 14(3):195-6.
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ABSTRACT: Immunoinfertility is one of several causes of infertility in humans. Although progress on antisperm immunity and infertility has advanced during the past three decades, the nature of a real antisperm antibody (ASA) is still poorly understood. Dozens of sperm antigens have been isolated and characterized in association with infertility. However, it is difficult to identify a single predominant target antigen that could interact with all the ASAs. There are some protective mechanisms preventing ASA production in males and females. As chronic infection, vasectomy and vasovasostomy, heavy metals, and testicular cancer and torsion may induce the production of ASAs, they may be responsible for decreased motility and sperm penetration of cervical mucus, and the blockage of the acrosome reaction and the sperm-egg interaction. Many ASA assay methods have been developed, each with advantages and disadvantages. Efforts for the treatment of ASA-mediated infertility have been attempted. However, current therapy for ASA-associated infertility is almost empiric and largely unproven.
Expert Review of Clinical Immunology 01/2008; 4(1):113-26. · 2.07 Impact Factor
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ABSTRACT: It may appear that problems of reproductive immunology have little in common with issues of treatment and prevention of AIDS. However, the certain aspects of HIV immunopathogenesis are closely related to problems faced by reproductive immunologists. The development of prophylactic and therapeutic AIDS vaccines would greatly benefit from acquired experience in immune regulation of reproductive dysfunction. The spermatozoa and HIV are foreign intruders that must enter the host cell, oocyte or T-lymphocyte, in order to start the replication process. The immune responses of the host organism against fertilized egg or HIV-impregnated lymphocyte must be similar, since in theory they are directed against alloantigens presented by such cells. This paper attempts to bring together the recent advances in AIDS field with progress made in the physiology and pathology of reproduction in humans, especially in the domain of immunotherapy and prevention of recurrent spontaneous abortions (RSA). It is our opinion that the lessons learned from alloimmunization trials of infertile women are relevant to prophylactic and therapeutic strategies for HIV infection.
Current Immunology Reviews 04/2007; 3(2):101-107.
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ABSTRACT: The determination of ACP in seminal plasma was considered as an appropriate biochemical marker to evaluate prostate function, as recommended by the WHO manual. However, few reports on the standardization and quality control for the determination of biochemical markers in seminal plasma have been documented.
Two frozen samples of seminal plasma with or without phenylmethylsulfonyl fluoride were determined for their acid phosphatase (ACP) levels. The ACP level and sperm concentration of each of 72 samples of seminal plasma obtained at 1000xg for 10 min or 3000xg for 15 min centrifugation were assayed. ACP activity in 10 samples of seminal plasma was measured immediately or standing for 30 min after dilution. The ACP levels in seminal plasma with or without chymotrypsin were also assayed.
There was no significant difference of ACP levels (P=0.166) but of sperm concentrations (P=0.000) in seminal plasma obtained by centrifugation at different velocity. ACP activities in seminal plasma measured when standing for 30 min after dilution were significantly lower than those measured immediately after dilution (P=0.001). Both chymotrypsin and freezing-thawing had no apparent effect on the determination of ACP in seminal plasma.
The results indicated that standing time after dilution and centrifugation velocity should be standardized, and frozen seminal plasma could serve as the quality control products for the determination of ACP activity among different laboratories.
Clinica Chimica Acta 02/2007; 375(1-2):76-81. · 2.54 Impact Factor
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ABSTRACT: B Baacckkggrroouunndd:: Sperm counts are currently being performed using a variety of methods that produce results that do not correlate well with each other or with the current "gold standard" reference method, hemacytometer. Significant differences in the accuracy of sperm counts using these different counting methods emphasize the need for standardization and quality control procedures to ensure adequate intermethod agreement between sperm counting methods. We compared sperm counts using 3 commonly used methods— hemacytomer, Cell-VU, and Makler—applied to both semen and sperm suspensions prepared from semen. M Meetthhooddss:: Semen samples were obtained by masturbation from 60 outpatient volunteers. Sperm suspensions containing low, medium, and high sperm concentrations were prepared from 38 of these semen samples. The results of sperm counts on semen and sperm suspension samples were analyzed with SPSS 11.0 software.
Labmedicine. 01/2007; 38(4):232-236.
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ABSTRACT: To evaluate the accuracy and precision of 4 methods including Hemacytometer, Makler chamber, Cell-VU chamber, and computer-aided semen analysis for determining sperm concentration.
Latex bead solutions with concentrations known as (35 +/- 5) x 10(6)/ml and (18.0 +/- 2.5) x 10(6)/ml and semen samples (n = 54) were counted by the above 4 methods and the results were then compared.
Mean bead concentrations for Hemacytometer, Makler, Cell-VU chambers and CASA were (44.84 +/- 4.86), (52.36 +/- 7.78), (39.70 +/- 4.76), (28.53 +/- 2.06) x 10(6)/ml respectively for the standard solution containing (35 +/- 5) x 10(6)/ml, and (21.04 +/- 1.87), (24.54 +/- 3.67), (19.09 +/- 2.02), (14.62 +/- 0.95) x 10(6)/ml respectively for a standard solution containing (18 +/- 2.5) x 10(6)/ml. The results of Cell-VU chamber were consistently similar and close to the standard solutions, while those of Hemacytometer, Makler chambers were overestimated, and those of CASA were underestimated. The coefficients of variation for Hemacytometer, Makler, Cell-VU chambers and CASA were 10.81%, 14.86%, 12.80%, and 7.22% respectively for a higher standard solution, while 8.89%, 14.96%, 10.58%, and 6.50% respectively for a lower standard solution. CASA has the lowest CV%. When semen samples were counted, the results of Hemacytometer, Makler, Cell-VU chambers and CASA were (76.98 +/- 59.90), (63.89 +/- 53.84), (45.28 +/- 34.52), (41.96 +/- 31.93) x 10(6)/ml respectively. There wasn't any significant difference either between Cell-VU chamber and CASA (P = 0.71), or between Hemacytometer and Makler chamber (P = 0.14), while there was significant difference between Cell-VU chamber or CASA and Hemacytometer or Makler chamber (P < 0.05 or P <0.01).
When counting semen sample, there wasnt any significant difference between Cell-VU chamber and CASA. Each laboratory can select its own proper method for manual or computer-aided analysis.
Zhonghua nan ke xue = National journal of andrology 03/2006; 12(3):222-4, 227.
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ABSTRACT: Few reports of the effects of treatment with chymotrypsin on the determination of sperm parameters and seminal biochemistry markers are documented.
Sperm parameters of 63 liquefied and 27 non-liquefied samples, untreated or treated with chymotrypsin, were evaluated using computer-assisted semen analysis. In addition, biochemistry markers such as gamma-glutamyltranspeptidase, alpha-glucosidase and fructose in 50 liquefied and 39 non-liquefied samples, untreated or treated with chymotrypsin, were determined.
Treatment with chymotrypsin had no effect on sperm concentration, motility, motility a and b, straightness, curvilinear velocity, straight line velocity, average path velocity and beat cross frequency in both liquefied and non-liquefied semen. However, linearity (p=0.025) decreased and the amplitude of the lateral head (p=0.029) increased significantly in non-liquefied semen after treatment with chymotrypsin. The levels of gamma-glutamyltranspeptidase, alpha-glucosidase and fructose in seminal plasma were unaffected by chymotrypsin, regardless of liquefaction status.
Chymotrypsin had no effects on the detection of sperm parameters and biochemistry markers, and could be used to treat non-liquefied samples before semen analysis in the andrology laboratory.
Clinical Chemistry and Laboratory Medicine 02/2006; 44(11):1335-9. · 2.15 Impact Factor
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ABSTRACT: The development of prophylactic AIDS vaccines and immunotherapeutic approaches such as therapeutic AIDS vaccines would greatly benefit from acquired experience in reproductive immunology field relating to pregnancy and infertility. Certain immune and pathological aspects of HIV infection are closely related to problems faced by obstetricians, embryologists, gynecologists, and andrologists. This review attempts to bring together the recent advances in AIDS field with progress made in the physiology and pathology of reproduction in humans. The insight gained in reproductive biology research, particularly in the area of immunotherapy of recurrent spontaneous abortions, can help to develop better strategies for the treatment and prevention of HIV infection.
Zhonghua nan ke xue = National journal of andrology 04/2005; 11(3):163-9.
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Zhonghua nan ke xue = National journal of andrology 02/2005; 11(1):7-8.
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ABSTRACT: This editorial briefly reviewed the history of andrology, its rapid development in recent years, the conception of male health, international andrology studies, the achievements of Chinese andrologists and the existing problems. It is highly necessary for Chinese andrology community to strengthen the international academic communication, join the International Society of Andrology and attend its activities actively.
Zhonghua nan ke xue = National journal of andrology 05/2003; 9(2):79-81.
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Journal of Andrology 28(2):207-13. · 2.97 Impact Factor
