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ABSTRACT: Electrophoretic patterns of serum proteins provide useful information on pathological conditions in ruminants. Their reference values, however, are dissimilar to those of other species. Reference values for goats using agarose gel as the supporting matrix have not been reported.
The aim of this study was to evaluate serum concentrations of total protein and protein fractions (albumin and globulins) by means of agarose gel electrophoresis (AGE) in goats in order to establish electrophoretic reference intervals and to evaluate potential changes associated with aging.
Blood was collected from 105 clinically healthy Girgentana goats by means of jugular venipuncture. Serum protein concentrations were assessed by AGE. Three age groups were compared: 1-1.5 years, 2-4 years, and 5-12 years.
Values (mean ± SD) were determined for concentrations of total protein (72.26 ± 6.40 g/L), albumin (31.80 ± 4.00 g/L), α-globulins (6.40 ± 1.23 g/L), β(1) -globulins (10.50 ± 2.58 g/L), β(2) -globulins (5.18 ± 1.60 g/L), and γ-globulins (18.65 ± 5.90 g/L) and for albumin/globulin (A/G) ratio (0.82 ± 0.20). One-way ANOVA showed statistically significant age-related differences for total protein and α-globulin concentrations and A/G ratios. Age influenced protein concentrations with the 5-12-year-old group having higher total protein and α-globulin concentrations and lower albumin concentration and A/G ratios than the 2-4-year-old group.
This study provides reference values for total protein concentrations and protein fractions obtained by AGE in goats. Some values vary with age. Age-specific reference intervals are reported in order to provide clinicians with an additional diagnostic aid.
Veterinary Clinical Pathology 04/2010; 39(3):317-21. · 1.56 Impact Factor
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Journal of Applied Animal Research 01/2009; 35(2):131-135. · 0.40 Impact Factor
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ABSTRACT: The aim of this study was to establish the influence of shearing in dairy sheep during summer season. Several physiological and haematological parameters, which are closely related to thermoregulatory potential, have been investigated with the purpose to assess shearing influence on thermoregulation. Forty dairy sheep, clinically healthy and well-fed, were used. They were divided into two groups of 20 subjects each. Twenty sheep were let unshorn as a control group (Group A), and twenty sheep were shorn (Group B). On each subject of group A and Group B, rectal temperature, respiration and heart rates were recorded and blood samples were collected in order to asses the following parameters: red blood cells, white blood cells, platelets, haematocrit and haemoglobin. All measurements taken on day 0 (before shearing), were repeated after 1, 15, 30, 45 and 60 days after shearing. The statistical analysis, the ANOVA followed by the Bonferroni's test, showed statistical differences between two groups for rectal temperature (F(1.90) = 5.57, p < 0.0001), respiratory rate (F(1.90) = 18.40, p < 0. 0001) and white blood cells (F(1.190) = 7.61, p < 0.0001). These results suggest that the shearing induce adaptative responses in the organism.
Journal of Environmental Biology 11/2008; 29(6):877-80. · 0.64 Impact Factor
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ABSTRACT: In equine sport medicine, blood clotting and fibrinolysis variations are well investigated, given the practical implications of several pathophysiological conditions affecting the athlete horse such as exercise-induced pulmonary haemorrhage (EIPH) and other bleeding disorders whose etiology and pathogenesis mechanisms are not yet clearly understood. The purpose of the present investigation was to gain evidence of a daily rhythm of several blood clotting indices such as prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), plasma fibrinogen concentration and serum vitamin K concentration in the athletic horses. Blood samples from five thoroughbred mares were collected at 4-h intervals for 48h (starting at 08:00h on day1 and finishing at 4:00 on day2 via an intravenous catheter inserted into the jugular vein. Prothrombin time, activated partial thromboplastin time, thrombin time and plasma fibrinogen concentration were assessed by means of a Seac Clot 2 coagulometer (SEAC, Italy), while serum vitamin K concentration was measured by HPLC. Data analysis was conducted by one-way repeated analysis of variance (ANOVA) and by the single cosinor method. ANOVA showed a significant influence of time on all parameters investigated, in all horses, on either day. Cosinor analysis defined the periodic parameters and their acrophases (expressed in hours) during the 2days of monitoring. PT showed a nocturnal acrophase, whereas serum vitamin K concentration acrophase occurred during the evening. The results of this study reflect the physiological peculiarities of the horse that is subjected to a number of exogenous (environmental, nutritional, physical) and endogenous stimuli capable of entraining the circadian rhythm specifically and thus producing time-dependent variations not always comparable with those observed in humans or laboratory animals.
Comparative Clinical Pathology 10/2005; 14(2):86-89.
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Veterinary Research Communications 10/2003; 27 Suppl 1:229-32. · 0.82 Impact Factor
