Zhou Xiang

Sichuan University, Hua-yang, Sichuan, China

Are you Zhou Xiang?

Claim your profile

Publications (66)78.69 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Endothelial progenitor cells (EPC) derived from the circulation may be used to enhance neovascularization. Since the combination of granulocyte colony-stimulating factor (GCSF) and CXCR4 antagonist AMD3100 efficiently mobilizes hematopoietic stem cells into peripheral circulation, it may increase the pool of endogenously circulating EPC. We tested this hypothesis by administering GCSF and AMD3100 to adult rabbits and rats, isolating mononuclear cells from peripheral blood by Ficoll density gradient centrifugation, and characterizing the blood-derived EPC based on morphology, immunophenotyping, gene expression and other functional analyses. These EPC showed clonal growth similar to that of human umbilical vein endothelial cells when cultured in complete EGM-2 medium on collagen I-precoated culture plates. The EPC exhibited a typical cobblestone-like morphology and were relatively homogeneous by the third passage. The cells expressed the typical endothelial marker CD31 based on flow cytometry and fluorescence microscopy, formed capillary-like structures when cultured in Matrigel, internalized DiI-acetylated low-density lipoprotein, bound Ulex europaeus agglutinin-1, and expressed CD31 and several other endothelial markers (VEGFR2, VE-cadherin, Tie-2, eNOS, vWF) at significantly higher levels than bone marrow-derived mesenchymal stem cells. These results suggest that the combination of GCSF and AMD3100 can efficiently release stem cells into peripheral circulation and generate EPC that show the desired morphological, immunophenotypic and functional characteristics. This minimally invasive approach may be useful for autologous cell transplantation for postnatal neovasculogenesis and tissue repair.
    Cell and Tissue Banking 07/2015; DOI:10.1007/s10561-015-9527-4 · 1.03 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effect of perioperative oral nutritional supplementation (ONS) on elderly patients after hip surgery remains controversial. This study intended to ascertain whether perioperative ONS is beneficial for the rehabilitation of elderly patients after hip surgery. We searched databases including PubMed, Embase, and the Cochrane Central Register of Controlled Trials for articles published up to May 2014. Randomized controlled trials of ONS for elderly patients after hip surgery were included. The combined trials showed that ONS had a positive effect on the serum total protein (P<0.00001) and led to a significantly decreased number of complications (P=0.0005). Furthermore, data from the infection subgroups showed significant decreases in wound infection (P=0.02), respiratory infection (P=0.04), and urinary tract infection (P=0.03). Clinical observation suggests that the intervention may improve the level of serum albumin, although the data did not reach statistical significance (P=0.48). Regarding mortality, there was no significant statistical difference between the intervention group and the control (P=0.93). Based on the evidence available, this meta-analysis is consistent with the hypothesis that perioperative ONS can help elderly patients recover after hip surgery and reduce complications.
    Clinical Interventions in Aging 05/2015; 10:849. DOI:10.2147/CIA.S74951 · 1.82 Impact Factor
  • Long Chen · Zhao Xu · Jing Peng · Fei Xing · Hong Wang · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the effectiveness and safety of arthroscopic and open Bankart repair for recurrent anterior shoulder dislocation using meta-analysis of data from clinical trials. Cochrane Register of Controlled Trials, PUBMED and EMBASE were used to search and identify clinical trials that evaluated arthroscopic and open Bankart repair for recurrent anterior shoulder dislocation. Methodological qualities of studies were assessed by Cochrane Collaboration tool for assessing risk of bias and Newcastle-Ottawa Scale. Publication bias was detected using Begg's test and Egger's test. Sixteen trials involving 827 shoulders were included in the study. Based on Cochrane Collaboration tool for assessing risk of bias, three studies were rated as high quality and one study was rated as moderate quality among the randomized controlled trials. Another twelve case-control studies were rated as high quality based on Newcastle-Ottawa Scale. No significant publication bias was detected by Begg's test or Egger's test. Meta-analysis results indicated that arthroscopic repair has a significantly better recovery rate for external rotation at 90° of abduction, external rotation at side (P > 0.05) and forward flexion. However, arthroscopic repair had higher rates of recurrence and reoperation than open Bankart repair. Meta-analysis of available randomized controlled trials and case-control studies demonstrated that arthroscopic repair and open Bankart repair were similar in safety. Arthroscopic repair resulted in better recovery of range of motion, but recurrence and reoperation rates were higher than open Bankart repair.
    Archives of Orthopaedic and Trauma Surgery 03/2015; 135(4). DOI:10.1007/s00402-015-2175-0 · 1.36 Impact Factor
  • D. Zhang · L. Chen · K. Peng · F. Xing · H. Wang · Z. Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: Repair of soft tissue in favor of the posterior approach for total hip arthroplasty is still under discussion and few studies are assessing this issue. Therefore we performed a meta-analysis to compare the effectiveness and safety of the posterior approach for total hip arthroplasty with and without soft tissue repair. We focus on these questions as follows: does primary posterior approach for total hip arthroplasty with soft tissue repair has better result regarding dislocation rate, Harris hip score and the sciatic nerve palsy rate compared with posterior approach without soft tissue repair.
    Revue de Chirurgie Orthopédique et Traumatologique 02/2015; 101(1). DOI:10.1016/j.rcot.2014.12.001
  • D. Zhang · L. Chen · K. Peng · F. Xing · H. Wang · Z. Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: Repair of soft tissue in favour of the posterior approach for total hip arthroplasty is still under discussion and few studies are assessing this issue. Therefore, we performed a meta-analysis to compare the effectiveness and safety of the posterior approach for total hip arthroplasty with and without soft tissue repair. We focused on these questions as follows: does primary posterior approach for total hip arthroplasty with soft tissue repair has better result regarding dislocation rate, Harris hip score and the sciatic nerve palsy rate compared with posterior approach without soft tissue repair.
    Orthopaedics & Traumatology Surgery & Research 01/2015; 101(1). DOI:10.1016/j.otsr.2014.10.015 · 1.17 Impact Factor
  • Kun Peng · Long Chen · Jing Peng · Fei Xing · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: To investigate whether calcitonin can improve walking distance (WD) and visual analog pain scale (VAS) in patients who suffer lumbar spinal stenosis (LSS). We performed a search on CENTRAL, PubMed, Embase and Cochrane databases up to July 2014; we finally found 19 original articles, of which only 6 were in full compliance with the RCT criteria. These full articles were carefully reviewed independent and in blinded way by two previously capacitated reviewers for the objective to extract data and score a quality of these articles by the criteria of Cochrane Handbook (5.1.0). We accepted 6 studies with 232 participants. There is no evidence show calcitonin is better than placebo or paracetamol regardless of mode of administration. This meta-analysis suggest that calcitonin provide no significant improvement in pain symptoms or walking distance in LSS patients.
    International Journal of Clinical and Experimental Medicine 01/2015; 8(2):2536-44. · 1.42 Impact Factor
  • Long Chen · Kun Peng · Dagang Zhang · Jing Peng · Fei Xing · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the effectiveness of early and delayed motion in rehabilitation after arthroscopic rotator cuff repair using a meta-analysis from randomized controlled trials. Electronic searches of the CENTRAL, PUBMED, and EMBASE were used to identify randomized controlled trials that evaluated the effectiveness and safety of early and delayed motion for rehabilitation after arthroscopic rotator cuff repair. The methodological quality of the studies was assessed by the Cochrane Collaboration tool for assessing risk of bias. Four randomized controlled trials involving a total of 348 shoulders were included. Of these, two were rated as high quality and two were rated as moderate quality. No significant publication bias was detected by Egger's test and sensitivity analysis demonstrates a statistically robust result. Our meta-analysis indicated that early motion after arthroscopic rotator cuff repair resulted in a significantly greater recovery of external rotation from pre-operation to 3, 6, and 12 months post-operation (P < 0.05) and forward elevation ability from pre-operation to 6 months post-operation (P < 0.05), as compared to when motion was delayed. However, early motion resulted in non-significant excess (P > 0.05) in the rate of recurrence, compared to delayed motion. In addition, there were statistically higher rating scale of the American Shoulder and Elbow Surgeons (ASES) scores at 12 months post-operation (P < 0.05) and healing rates (P < 0.05) with delayed motion after arthroscopic rotator cuff repair, compared with early motion. Our meta-analysis included data from randomized controlled trials and demonstrated that delayed motion after arthroscopic rotator cuff repair resulted in higher healing rates and ASES scores than early motion. Alternatively, early motion increased range of motion (ROM) recovery, but also increased the rate of recurrence compared to delayed motion.
    International Journal of Clinical and Experimental Medicine 01/2015; 8(6):8329-38. · 1.42 Impact Factor
  • Jing Peng · Li Min · Zhou Xiang · Fuguo Huang · Chongqi Tu · Hui Zhang
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the curative effect of Ilizarov bone transport combined with antibiotic cement spacer for infected tibial nonunion with bone defect. We retrospectively reviewed the outcomes of 58 patients with infected tibial nonunion from January 2008 to March 2011 at our institution. Patients were treated with complete debridement, radical sequestrectomy, antibiotic cement spacer implantation, bone transport using the Ilizarov external fixator, and soft tissue reconstruction. Clinical efficacy was assessed using Paley's grading system and patient satisfaction at the last follow-up. Follow-up ranged from 24 to 63 months (average, 31.6 months). Mean size of the tibial defect was 9.2 cm (range, 6-15 cm). The soft tissue defect was closed successfully in all cases. Patients eventually achieved union with a mean bone union index of 1.2 months/cm at an average of 10.6 months (range, 8-31 months). In terms of Paley grade, 30 patients had excellent results, 23 good, and 5 fair. Functional results were excellent in 28 patients, good in 18, and fair in 12. Thirty-five patients felt extremely satisfied, 18 satisfied, and 5 acceptable with the functional outcome. Complications included pin site infection in 18 cases, limb length discrepancy less than 1.5 cm in 10, knee stiffness in 5, equinus deformity in 4, infectious recurrence in 1 and pin breakage in 1. There was no refracture at the reconstruction site. Ilizarov bone transport combined with antibiotic cement spacer is a versatile and effective method for treatment of infected tibial nonunion.
    International Journal of Clinical and Experimental Medicine 01/2015; 8(6):10058-65. · 1.42 Impact Factor
  • Z Xu · X Duan · X Yu · H Wang · X Dong · Z Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: To determine the accuracy of MRI versus ultrasound for Morton's neuroma. A search was undertaken for clinical studies published in any language in PubMed up to the date of December 2013. Studies assessing the accuracy of the ultrasound or MRI for the diagnosis of Morton's neuroma were included. Data were pooled for meta-analysis. Study selection, data collection, and extraction were performed independently by two authors. Meta-disc 1.4 and Revman 5.2 software were applied for statistical analysis. The study included 12 studies; 217 patients underwent MRI and 241 underwent ultrasound examinations. There appeared greater diagnostic accuracy for ultrasound than MRI for the diagnosis of Morton's neuroma (ultrasound sensitivity 90%, specificity 88%, positive likelihood ratio 2.77, negative likelihood ratio 0.16 versus MRI sensitivity 93%, specificity 68%, positive likelihood ratio 1.89, negative likelihood ratio 0.19). The available evidence suggests that ultrasound can provide better accuracy for the diagnosis of Morton's neuroma than MRI. Copyright © 2014 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.
    Clinical Radiology 11/2014; 70(4). DOI:10.1016/j.crad.2014.10.017 · 1.66 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Vascularization of engineered bone tissue is critical for ensuring its survival after implantation, and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bone grafts in vitro using endothelial progenitor cells (EPC) derived from peripheral blood. Typically EPC cells are added together with mesenchymal stem cells (MSC) that differentiate into osteoblasts. One problem with this approach is how to promote traditional tissue engineering bone survival with a minimally invasive approach method. Here we examined the effectiveness of administering to stimulate the release of peripheral blood stem cells. Cells were isolated by Ficoll density gradient centrifugation and identified as EPC and MSC based on morphology, surface markers, and functional analysis. EPC and MSC were co-cultured in several different ratios, and cell morphology and tube formation were assessed by microscopy. Expression of osteogenesis and vascularization markers was quantified by ELISA, PCR, histochemical and immunofluorescence staining. Increasing the proportion of EPC in the co-culture system led to greater tube formation and greater expression of endothelial cell marker CD31. An EPC:MSC ratio of 75:25 gave the highest expression of osteogenesis and angiogenesis markers. Co-cultures adhered to a three-dimensional scaffold of strontium-doped calcium polyphosphate and proliferated well. Our findings show that co-culturing peripheral blood-derived EPC and MSC may prove useful for generating prevascularized bone tissue for clinical use.
    Tissue Engineering Part A 10/2014; 21(5-6). DOI:10.1089/ten.TEA.2014.0267 · 4.70 Impact Factor
  • Xi Liu · Shiqiang Cen · Fuguo Huang · Zhou Xiang · Gang Zhong
    International Journal of Surgery 10/2014; 13. DOI:10.1016/j.ijsu.2014.09.011 · 1.65 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the specific microRNA (miRNA) in osteogenic and chondrogenic differentiations of C3H10Tl/2 cells. C3H10Tl/2 cells were induced to differentiate into osteoblasts and chondrocytes. Specific miRNA more than 2 fold change and 2 average normalized probe signal between C3H10Tl/2 and C3H10Tl/2-derived osteoblast, and' between C3Hl0Tl/2 and C3H10Tl/2-derived chondrocytes were screened out by miRNA microarray, and verified by real-time fluorescence quantitative PCR (RT-qPCR). Alkaline phosphatase expression of osteogenic induced group was significantly higher than that of control group at 7 days after induced (P < 0.05). RT-qPCR results showed the expressions of Runx2, serine protease (Sp7), collagen type I, and osteopontin (OPN) genes were significantly increased at 7, 14, and 21 days after induced when compared with before induced (P < 0.05). Western blot results showed the expressions of Runx2, Sp7, collagen type I, and OPN proteins of osteogenic induced group were significantly higher than those of control group at 21 days after induced (P < 0.05). The expressions of SOX9, collagen type II, Aggrecan, and Has2 were significantly increased at 5, 10, and 15 days after induced when compared with before induced (P < 0.05). The expressions of SOX9, collagen type 2, Aggrecan, and Has2 proteins of chondrogenic induced group were significantly higher than those of control group at 15 days after induced (P < 0.05). Totally, 10 osteogenic and 3 chondrogenic miRNA more than 2 fold change and 2 average normalized probe signal were screened out by miRNA microarray. RT-qPCR results of these specific miRNAs were similar to microarray results except miR-455-3p. Specific miRNAs are screened out by microarray and it is a good foundation for the future study on miRNA functional verification and target gene prediction.
  • J Yang · H J Chen · X D Zhu · S Vaidya · Z Xiang · Y J Fan · X D Zhang
    [Show abstract] [Hide abstract]
    ABSTRACT: Repair of load-bearing bone defects remains a challenge in the field of orthopaedic surgery. In the current study, a surface microstructured porous titanium (STPT) successively treated with H2O2/TaCl5 solution and simulated body fluid was used to repair the critical-sized segmental bone defects in rabbit femur, and non-treated porous titanium (NTPT) and porous biphasic calcium phosphate ceramics (PBCP) were used as control, respectively. A 15 mm long implant was positioned in the femoral defect and stabilized by a plate and screws fixation. After implantation into the body for 1, 3 and 6 months, X-ray observation confirmed that porous titanium groups (NTPT and STPT) provided better mechanical support than PBCP group at the early stage. However, there was no obvious difference in the formed bony callus between PBCP and STPT groups in the later stage, and they both showed better shape of bony callus than NTPT group. Micro-CT and histomorphometric analysis for the samples of 6-month implantation demonstrated that more new bone formed in the inner pores of PBCP and STPT groups than that in NTPT group. Moreover, the biomechanical tests revealed that STPT group could bear larger compressive load than NTPT and PBCP groups, almost reaching the level of the normal rabbit femur. STPT exhibited the enhanced repairing effect on the critical-sized segmental bone defect in rabbit femur, meaning that it could be an ideal material for the repair of large bone defect in load-bearing site.
    Journal of Materials Science Materials in Medicine 03/2014; 25(7). DOI:10.1007/s10856-014-5202-8 · 2.38 Impact Factor
  • Xiao Huang · Frankie Leung · Ming Liu · Long Chen · Zhao Xu · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: This meta-analysis was conducted to investigate whether helical blade implant system had advantages in terms of cut-out rate when compared to screw implant system for trochanteric fractures of elderly population. The databases of MEDLINE, Cochrane Library and OVID were searched from inception to September 2013, and all randomized controlled trials comparing outcomes between helical blade system and screw system in treating trochanteric fractures were selected. Three researchers assessed the methodological quality and extracted data of the enrolled studies independently. Data were analysed using Review Manager 5.1 version. Six studies including 759 patients were involved. Results revealed that compared with screw group, blade group had similar outcomes of "centre-centre" position (95 % CI 0.89-1.06, P = 0.48) and tip-apex distance (95 % CI-0.08 to 1.31, P = 0.08). Cut-out and other complications were also comparable between the two groups (95 % CI 0.34-1.54, P = 0.41; 95 % CI 0.73-1.32, P = 0.90). Operation time and fluoroscopy time of blade group were significantly less than that of screw group (95 % CI -5.13 to -3.70, P < 0.00001; 95 % CI -32.50 to -27.07, P < 0.00001). Outcome of post-operative function was similar between two groups (95 % CI 0.94-1.15, P = 0.45). Blade group required less operation time and fluoroscopy time than that of screw group treating trochanteric fractures in the elderly, but the differences observed could be biased due to grouping and other limitations. Outcomes of cut-out complication, other complications, position of implant and post-operative function were similar between two groups.
    European Journal of Orthopaedic Surgery & Traumatology 02/2014; 24(8). DOI:10.1007/s00590-014-1429-9 · 0.18 Impact Factor
  • Weili Fu · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: To review the research progress of the co-culture system for constructing vascularized tissue engineered bone. The recent literature concerning the co-culture system for constructing vascularized tissue engineered bone was reviewed, including the selection of osteogenic and endothelial lineages, the design and surface modification of scaffolds, the models and dimensions of the co-culture system, the mechanism, the culture conditions, and their application progress. The construction of vascularized tissue engineered bone is the prerequisite for their survival and further clinical application in vivo. Mesenchymal stem cells (owning the excellent osteogenic potential) and endothelial progenitor cells (capable of directional differentiation into endothelial cell) are considered as attractive cell types for the co-culture system to construct vascularized tissue engineered bone. The culture conditions need to be further optimized. Furthermore, how to achieve the clinical goals of minimal invasion and autologous transplantation also need to be further studied. The strategy of the co-culture system for constructing vascularized tissue engineered bone would have a very broad prospects for clinical application in future.
    Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 02/2014; 28(2):179-85.
  • Jingtong Lyu · Xiaojing Wu · Xin Duan · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract With the evergrowing number of people who work in visual display terminal, the work-related musculoskeletal disorders of the upper body are believed to be an important problem all over the world. The forearm support,which can keep the forearm and wrist in biomechanical posture, is a possible protective factor of the development of upper body syndrome. This meta-analysis examines the efficacy of forearm support in reducing upper body syndrome. The Cochrane Library, EMBASE, Ovid, Science direct, Springer-link, Google Scholar, CNKI database, Wanfang database were searched from inception until May 29th 2013. Studies relevant were included after the screen of title, abstract and the full text. Impact of bias was assessed independently by two authors.Four studies that met all the inclusion criteria were included finally. The combined results based on all studies suggested that statistically the forearm support had a unconspicuous effect on upper body syndrome (OR = 0.70, 95% CI: 0.49, 1.02).The result of subgroup suggested that forearm support have a significant effect on neck or shoulder syndrome (OR = 0.70, 95% CI: 0.43, 1.14) and the effect on upper extremity syndrome(OR = 0.76, 95% CI: 0.49, 1.19) is not significant. This meta-analysis suggested that the forearm support had statistically unconspicuous effect on preventing upper body syndrome on the whole.
    Archives of Environmental and Occupational Health 01/2014; DOI:10.1080/19338244.2013.845138 · 0.47 Impact Factor
  • Long Chen · Xin Duan · Xiao Huang · Jingtong Lv · Kun Peng · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the effectiveness and safety of endoscopic carpal tunnel release (ECTR) and open carpal tunnel release (OCTR) using a meta-analysis of data from randomized controlled trials. Electronic searches of the Cochrane Register of Controlled Trials (CENTRAL, Issue 11 of 12, Nov 2012), PUBMED (1980 to Dec 2012), and EMBASE (1980 to Dec 2012) were used to identify randomized controlled trials that evaluated endoscopic vs open methods for treatment of carpal tunnel syndrome. Studies to be used were independently identified by two researchers. The methodological quality of the studies was assessed by the Cochrane Collaboration tool for assessing risk of bias. Fifteen randomized controlled trials involving 1,596 hands were included. Based on the Cochrane Collaboration tool for assessing risk of bias, four studies were rated as high quality, five studies were rated as moderate quality, and six were rated as low quality. Our meta-analysis indicated that ECTR resulted in better recovery of pinch strength, earlier time of return to work, but a higher rate of reversible nerve problems (including neurapraxia and numbness) than OCTR. ECTR also resulted in a lower rate of irreversible nerve damage (P > 0.05), wound problems (including wound infection, wound hematoma and wound dehiscence) and reflex sympathetic dystrophy (P > 0.05) compared with OCTR. Our meta-analysis revealed no obvious statistical differences in relief of symptoms (pain and paraesthesia), recovery of grip strength and reoperation rate. Our meta-analysis of available randomized controlled trials demonstrated that ECTR and OCTR were similar in relief of symptoms, but ECTR resulted in better recovery of function and earlier return to work and was safer than OCTR.
    Archives of Orthopaedic and Trauma Surgery 01/2014; 134(4). DOI:10.1007/s00402-013-1898-z · 1.36 Impact Factor
  • Ming Liu · Xi Yu · Fuguo Huang · Shiqiang Cen · Gang Zhong · Zhou Xiang
    [Show abstract] [Hide abstract]
    ABSTRACT: EDUCATIONAL OBJECTIVES As a result of reading this article, physicians should be able to: 1. Analyze the latest innovations in biomaterial scaffolds for the repair and regeneration of osteochondral defects. 2. Discuss the design and manufacture of biomaterial scaffolds. 3. Compare the advantages and disadvantages of stratified and nonstratified scaffolds. 4. Review the latest fixation methods of scaffolds in vivo. Due to their good biocompatibility and mechanical integrity, tissue engineering scaffolds have become a principal method of repair and regeneration of osteochondral defects. To improve their intrinsic properties, control their degenerative times, and enhance their cell adhesion and differentiation, numerous scaffold architectures and formation methods have been developed and tested, but the ideal scaffold design is still controversial. Moreover, scaffold fixation has a significant influence on repair and regeneration after implantation. The authors analyzed relative studies to address the latest scaffold designs, including biphasic scaffold, multilayered scaffold, and continuous nonstratified scaffold, and this article compares their advantages and disadvantages. In addition, the authors introduce a novel modified method for scaffold fixation known as magnetic fixation. Both stratified and nonstratified scaffolds can repair osteochondral defects, but continuous nonstratified scaffolds are more biomimetic compared with the native osteochondral structures, and they lead to a better regeneration of hyaline-like cartilage and structured bone tissue. Therefore, the authors suggest continuous nonstratified scaffolds are an effective option for treating osteochondral defects.
    Orthopedics 11/2013; 36(11):868-73. DOI:10.3928/01477447-20131021-10 · 0.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The limited repair potential of articular cartilage, which hardly heals after injury or debilitating osteoarthritis, is a clinical challenge. The aim of this work was to develop a novel type I collagen (Col)/glycosaminoglycan (GAGs)-porous titanium biphasic scaffold (CGT) and verify its ability to repair osteochondral defects in an animal model with bone marrow stem cells (bMSCs) in the chondral phase. The biphasic scaffold was composed of Col/GAGs as chondral phasic and porous titanium as subchondral phasic. Twenty-four full-thickness defects through the articular cartilage and into the subchondral bone were prepared by drilling into the surface of the femoral patellar groove. Animals were assigned to one of the three groups: 1) CGT with bMSCs (CGTM), 2) only CGT, and 3) no implantation (control). The defect areas were examined grossly, histologically and by micro-CT. The most satisfied cartilage repairing result was in the CGTM group, while CGT alone was better than the control group. Abundant subchondral bone formation was observed in the CGTM and CGT groups but not the control group. Our findings demonstrate that a composite based on a novel biphasic scaffold combined with bMSCs shows a high potential to repair large osteochondral defects in a canine model.
    10/2013; 33(7):3951-7. DOI:10.1016/j.msec.2013.05.040
  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the protocols of combined culture of human placenta-derived mesenchymal stem cells (HPMSCs) and human umbilical vein endothelial cells (HUVECs) from the same and different individuals on collagen material,to provide the. Under voluntary contributions, HPMSCs were isolated and purified from human full-term placenta using collagenase IV digestion and lymphocyte separation medium, and confirmed by morphology methods and flow cytometry, and then passage 2 cells were cultured under condition of osteogenic induction. HUVECs were isolated from fresh human umbilical vein by collagenase I digestion and subcultured to purification, and cells were confirmed by immunocytochemical staining of von Willebrand factor (vWF). There were 2 groups for experiment. Passage 3 osteoblastic induced HPMSCs were co-cultured with HUVECs (1:1) from different individuals in group A and with HUVECs from the same individual in group B on collagen hydrogel. Confocal laser scanning microscope was used to observe the cellular behavior of the cell-collagen composites at 1, 3, 5, and 7 days after culturing. Flow cytometry showed that HPMSCs were strongly positive for CD90 and CD29, but negative for CD31, CD45, and CD34. After induction, alizarin red, alkaline phosphatase, and collagenase I staining were positive. HUVECs displayed cobble-stone morphology and stained positively for endothelial cell marker vWF. The immunofluorescent staining of CD31 showed that HUVECs in the cell-collagen composite of group B had richer layers, adhered and extended faster and better in three-dimension space than that of group A. At 7 days, the class-like microvessel lengths and the network point numbers were (6.68 +/- 0.35) mm/mm2 and (17.10 +/- 1.10)/mm2 in group A, and were (8.11 +/- 0.62) mm/mm2 and (21.30 +/- 1.41)/mm2 in group B, showing significant differences between the 2 groups (t = 0.894, P = 0.000; t = 0.732, P = 0.000). Composite implant HPMSCs and HUVECs from the same individual on collagen hydrogel is better than HPMSCs and HUVECs from different individuals in integrity and continuity of the network and angiogenesis.
    Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 08/2013; 27(8):916-22.