-
[show abstract]
[hide abstract]
ABSTRACT: The aim of this study was to enumerate and identify bifidobacteria from occlusal carious lesions in permanent and deciduous teeth. Samples of infected dentine were obtained from 24 active occlusal lesions in deciduous teeth and from 15 occlusal lesions in permanent teeth. Plaque samples from sound occlusal surfaces of 12 caries-free adults and 12 children were also obtained. The bifidobacterial strains were isolated in mupirocin-containing selective media, Gram-stained and subcultured for identification. Total bacterial counts were determined using fastidious anaerobic agar, and isolates were identified using genus-specific PCR primers and were confirmed by 16S rRNA sequencing. Bifidobacteria were isolated from 13 of the 15 occlusal lesions in the adults and formed 5.09 +/- 2.11% of the total cultivable flora. In the children, bifidobacteria were isolated from 16 of the 24 occlusal lesions and formed 7.4 +/- 2.6% of the total flora. No bifidobacteria were isolated from the occlusal surfaces of caries-free adults or children. A total of 424 bifidobacteria were identified and these were Bifidobacteriumdentium, Parascardovia denticolens, Scardoviainopicata, Bifidobacterium longum, Scardovia genomosp. C1 and Bifidobacterium breve. B. dentium was present in 14 out of the 16 bifidobacteria-positive samples from the lesions on the deciduous teeth and in 7 out of the 13 positive lesions in adults (p = 0.04). The present data suggest that bifidobacteria may play a role in the progression of occlusal caries lesions in both children and adults.
Caries Research 07/2009; 43(4):308-13. · 2.33 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To determine the antimicrobial properties of a selection of dentine bonding agents [DBAs] using the disc diffusion and direct contact methods and an ex vivo method using extracted carious permanent molar teeth.
DBAs (n=15) were tested using Streptococcus mutans UA159 in disc diffusion and direct contact methods. In the ex vivo study 6 DBAs were selected and pre- and post-treatment samples of carious dentine (n< or =12) were taken. Samples were also taken post-acid-etching. The number of microorganisms in dentine sample was determined and compared.
The inhibition zones and percent growth inhibition were related to the pH of the culture medium containing the DBA (p<0.01). Clearfill Protect Bond exhibited the greatest bacterial killing followed by ibond (99.8%+/-0.08 and 98.2+/-1.4, respectively). The phosphoric acid etchant alone resulted in an 83% killing. The in vitro tests results did not correlate. The ex vivo killing reflected the percent growth inhibition observed in the direct contact method.
A guide to the potential antimicrobial activity of a DBA may be gained from an assessment of its pH when added to bacteriological culture medium. The direct contact method gives a better reflection of the killing of bacteria in infected dentine than the disk diffusion method. Killing in the ex vivo model gives a more realistic and more reliable method for determining the antibacterial activity of a given DBA and that comparisons of the relative inhibitory activity of DBAs should be tested using this ex vivo model.
Journal of dentistry 04/2009; 37(7):514-21. · 2.00 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Detailed data on the distribution of Veillonella in caries-free and caries-active subjects are scarce. We hypothesized that the diversity of the genus would be lower in caries lesions than in plaque from caries-free individuals. The proportions of Veillonella were not significantly different in the two groups. All isolates (n = 1308) were genotyped by REP-PCR, and different genotypes (n = 170) were identified by 16S rRNA, dnaK, and rpoB sequencing. V. parvula, V. dispar, and V. atypica were in both groups, V. denticariosi only in caries lesions, and V. rogosae only from the caries-free individuals (p < 0.009). Lesions were more likely to harbor a single predominant species (p = 0.0018). The mean number of genotypes in the lesions was less than in the fissure (p < 0.001) or buccal (p = 0.011) sites. The Veillonella from caries-free sites were more diverse than those from caries lesions, and may be related to the acidic environment of caries lesions.
Journal of Dental Research 03/2008; 87(3):278-82. · 3.49 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Sotos syndrome, or cerebral gigantism, is a rare genetic condition characterized by tall stature, gigantism, dolichocephaly, advanced bone age and learning disability. The purpose of this case report is to highlight the dental management of a 10-year-old boy with Sotos syndrome who presented with hypodontia and dental caries.
International Journal of Paediatric Dentistry 04/2006; 16(2):143-6. · 1.01 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Conventional cultural methods were used to compare the plaque flora and the level of infection of the dentine underlying 51 occlusal brown-spot lesions and 21 sound occlusal sites on the primary dentition. Freshly extracted primary molar teeth were used, and occlusal brown-spot lesions and sound occlusal sites were identified using laser fluorescence (LF) and clinical visual methods. A standardized plaque sample was taken from each site, and an LF score was recorded for one discrete site per tooth. The teeth were carefully opened at each predetermined site to determine the clinical status of the underlying dentine, and samples were collected using a sterile bur. The microbiota of the plaque and dentine samples were enumerated and identified. The mean LF scores for the sound sites and brown-spot lesions were 1.2 and 30.5 (p < 0.001), and all the sound sites exhibited hard sound dentine, but 6 out of 51 brown spots exhibited softened dentine. Overall there was no significant (p > 0.1) difference between the level of infection of the dentine of the sound and brown-spot sites, although some sites in the brown-spot lesions yielded high numbers of bacteria. However, the numbers of bacteria as log10(CFU per sample + 1) +/- SE recovered from the plaque above the brown-spot lesions were significantly greater than above the sound sites, i.e. 2.89 +/- 0.24 and 0.89 +/- 0.33, respectively. These data indicate that brown-spot lesions may be more plaque retentive than sound sites and that they are either arrested or arresting lesions, which may require preventive intervention.
Caries Research 01/2006; 40(5):398-402. · 2.33 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This was to assess the effectiveness and safety of intraligamental local analgesia (ILA) for postoperative pain control in children having dental extractions under general anaesthesia (GA). The variables affecting the effectiveness of ILA were also investigated.
Data were collected from children having permanent molars extracted under GA using a randomised half mouth study design. ILA (bupivicaine 0.5% with 1:200,000 adrenaline) was used on the randomly assigned experimental side prior to extraction of the teeth, and the contralateral control side received no ILA. Children were interviewed pre- and post-operatively by the principal investigator (PA) who was blind to the side of ILA. Pre- and post-operative anxiety levels of each child were measured using the Venham Picture Test (VPT). Patients were asked to rate which side was better in terms of pain control, whether they felt numbness and whether they preferred the numbness. Their post-operative pain levels were also measured using the visual analogue scale (VAS). Self-inflicted trauma following ILA was noted.
Thirty children, with a mean age of 11.3 years (SD+/-1.7) completed the study. None of the patients had self-inflicted soft tissue trauma following ILA. Nineteen children (63%) found that pain control was better post-operatively on the side with ILA. Twenty-one children (70%) reported numbness following ILA of whom 14 (67%) said that they preferred this. VAS scores were not significantly different between the experimental and control sides. A higher percentage of boys (85%) than girls (47%) rated the ILA side "better" (p = 0.034). VPT scores were significantly higher for girls postoperatively (p = 0.048).
ILA was a useful and safe adjunct for postoperative pain control in children having permanent teeth extracted under GA. The technique was found to be more effective in boys than girls. However, less than half the children in this study preferred the side with numbness. Further research is needed to determine effective methods of pain control in children following dental GA and to investigate the reasons for gender differences of pain perception in children.
European Journal of Paediatric Dentistry. Official Journal of the Italian Society of Paediatric Dentistry. 04/2005; 6(1):10-5. · 0.44 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A case of a nasopalatine duct cyst is reported in an 8-year-old. The clinical presentation was of intermittent palatal swelling and the diagnosis was confirmed with radiological and histological examination. This type of cyst is rare in children and should not be excluded from a differential diagnosis in younger age groups.
International Journal of Paediatric Dentistry 04/2001; 11(2):135-7. · 1.01 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A case of a nasopalatine duct cyst is reported in an 8-year-old. The clinical presentation was of intermittent palatal swelling and the diagnosis was confirmed with radiological and histological examination. This type of cyst is rare in children and should not be excluded from a differential diagnosis in younger age groups.
International Journal of Paediatric Dentistry 02/2001; 11(2):135 - 137. · 1.01 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This report describes the dental management of a child with LEOPARD syndrome who presented with multiple grossly carious primary teeth. Comprehensive dental care was carried out under general anaesthesia.
International Journal of Paediatric Dentistry 07/2000; 10(2):158-60. · 1.01 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To determine the prevalence of dental caries in children undergoing liver transplantation and to compare the plaque, gingivitis, and gingival overgrowth indices and oral mucosal lesions in children before and after liver transplantation.
Patients undergoing liver transplantation at King's College Hospital were examined before transplantation, at 3 and 106 days post-transplantation. Healthy children from the Greenwich Health District, south-east London, were matched to the liver transplant patients by age, gender, socio-economic factors and the presence or absence of active dental caries.
Twenty-seven liver transplant patients (mean age: 7 years and 10 months; SD 3 years and 5 months) and 27 controls (mean age: 8 years and 6 months; SD 3 years and 7 months) were examined. The mean dmft and DMFT scores were 2.3 (SD 4.1) and 0.8 (SD 1.4), respectively, in the liver transplant patients, and 1.2 (SD 2.2) and 0.9 (SD 1.5), respectively, in the controls. There were no significant differences between either the mean plaque or gingivitis indices for the primary and permanent teeth in patients before and after transplantation. There were no significant differences between the liver transplant patients and the controls for either mean plaque or gingivitis indices at each examination time. Gingival overgrowth was present in 41% of liver recipients receiving cyclosporin with or without nifedipine, but not in the majority receiving tacrolimus at the final examination. Oral mucosal lesions were absent in both the patients and controls at each examination time.
The oral health of the children undergoing liver transplantation was inadequate. Funding and implementation of an oral health care programme must become a priority for all children before and after liver transplantation.
International Journal of Paediatric Dentistry 07/2000; 10(2):109-19. · 1.01 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This study investigated the oral microbiota of children undergoing liver transplantation. Oral swabs were taken using a standardized procedure from 27 children before liver transplantation and at 3 and 100 days post-transplantation and from 27 healthy controls at baseline and 90 days. Viridans streptococci, yeasts, staphylococci, enterococci and Enterobacteriaceae were enumerated and identified using conventional techniques. The oral microbiota of the patients changed significantly immediately post-transplantation, but by the final examination, it had returned to baseline levels. The oral microbiota of the controls did not change significantly. The numbers and proportions of Streptococcus salivarius, Streptococcus sanguis and Streptococcus gordonii as percentages of the total streptococcal counts and of the total anaerobic counts decreased significantly 3 days post-transplantation (P < or = 0.006). There were no significant changes in the numbers and proportions of Streptococcus oralis and Streptococcus mitis isolated pre- and post-transplantation. The isolation frequencies and numbers of yeasts were significantly higher in patients than controls. Staphylococci were isolated in low numbers from all children. Enterococci and Enterobacteriaceae were isolated infrequently from patients.
Oral Microbiology and Immunology 06/2000; 15(3):203-10. · 2.81 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Hallervorden-Spatz disease (HSD) is a rare neurodegenerative disorder characterized by abnormally high deposits of iron in the brain. This report describes a child with HSD who presented with self-inflicted ulceration of the lip and tongue, which was initiated during periods of intense oro-facial spasms. Other findings included dental caries and trauma to the primary incisors. Comprehensive dental care was carried out under general anaesthesia. The self-mutilation of the oro-facial mucosa was eliminated by placement of upper and lower soft resin bite guards.
International Journal of Paediatric Dentistry 01/2000; 9(4):299-302. · 1.01 Impact Factor
-
British dental journal 12/1999; 187(10):525. · 0.92 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To review the use of calcium hydroxide for induction of apical barrier formation and healing in immature permanent teeth.
Pulp necrosis is a frequent complication of dental trauma in immature permanent teeth. Endodontic treatment of these teeth is often complicated. The walls of the root canals are frequently divergent and the apices immature, making debridement and obturation difficult. The aim of treatment is induction of apical healing which may be defined as apical closure through formation of mineralised tissue and repair of the periapical tissues. Calcium hydroxide is the material of choice for apical barrier formation and healing.
The use of calcium hydroxide for apical barrier formation is successful in 74-100% of cases irrespective of the proprietary brand used. The average length of time for apical barrier formation is approximately 5 to 20 months. Control of infection and adequate cleaning of the root canal are very important for apical healing.
While the success rate of apical barrier formation using calcium hydroxide is high, long-term follow-up of these teeth is necessary. Problems such as failure to control infection, recurrence of infection and cervical root fracture may occur. The latter is more frequent in immature luxated teeth with the least root development.
British dental journal 11/1997; 183(7):241-6. · 0.92 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This paper presents the dental findings of a child with the oculocerebrorenal syndrome of Lowe. The genetic abnormality in this condition results in an inborn error of inositol phosphate metabolism. Renal tubular dysfunction leads to metabolic acidosis and phosphaturia. At 4 years, generalised mobility of all primary teeth was noted. It is postulated that a defective inositol phosphate metabolism was responsible for the periodontal pathology found in this case. This is in direct contrast with previous reports of prolonged retention of primary teeth in children with this condition. Histology of extracted primary incisors demonstrated enlarged pulp chambers and mildly dysplastic dentin formation. This is consistent with a chronic subrachitic state, a known feature of Lowe syndrome, but no prominent interglobular dentin was present.
Pediatric dentistry 21(7):425-8. · 1.02 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The number of pediatric liver transplantations undertaken in the US has increased dramatically in recent years. As the survival of liver recipients continues to improve, the dentist will need to be familiar with the management of these patients. This article describes the indications for pediatric liver transplantation, the types of liver transplants, the clinical features of liver disease, and the medical and dental management of children before and after liver transplantation.
Pediatric dentistry 21(4):272-80. · 1.02 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This study compared a laser fluorescence (LF) system (DIAGNOdent) with a visual caries scoring system for in vivo detection and diagnosis of occlusal caries under the conditions of an epidemiological study, in 132 mandibular and 38 maxillary first permanent molars in 170 children (mean age: 6.85 +/- 0.58 years). The teeth were cleaned and occlusal caries status in a selected investigation site recorded using both visual and LF systems. The LF readings were interpreted both according to the cut-off points recommended by the manufacturers and those based on laboratory research with histological validation. The percentage agreement of the LF and visual scoring system was better using the cut-off limits recommended by the manufacturer. Histological validation was not possible in this clinical study, but it appeared that either the LF method was overscoring some lesions or the visual method was underscoring them. Since the LF instrument cannot be expected to differentiate caries from hypomineralisation, it should probably be used as an adjunct to a clinical examination.
Caries Research 35(6):421-6. · 2.33 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Localized macrodontia is very rare. The majority of cases have been reported in mandibular second premolars. This paper describes the dental management of a child who presented with macrodontia of a mandibular right second premolar.
ASDC journal of dentistry for children. 68(5-6):347-9, 302.
-
[show abstract]
[hide abstract]
ABSTRACT: The relationship between microflora, eruption status and caries status in the first permanent molar of young children was investigated in 177 children aged 6-7 years. A significantly greater proportion of fully erupted teeth were classified as sound and plaque-free compared to partially erupted teeth. Fully erupted teeth yielded greater numbers and proportions of mutans streptococci compared with significantly greater numbers and proportions of Actinomyces israelii in partially erupted teeth. Logistical regression analysis showed significant associations between white spot lesions in partially erupted teeth and increased numbers of Streptococcus oralis, mutans streptococci and Streptococcus salivarius whereas the presence of Actinomyces naeslundii was associated with health. Significantly greater numbers and proportions of S. oralis and S. salivarius were isolated from partially erupted teeth with white spot lesions whereas Streptococcus mutans was isolated in significantly greater numbers and proportions from fully erupted molars with white spots. This study suggests that organisms other than mutans streptococci are associated with caries development in erupting permanent molar teeth.
Caries Research 39(1):78-84. · 2.33 Impact Factor
