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ABSTRACT: Abstract To obtain the characteristic variation of structure and functional groups of α-fetoprotein (AFP) DNA irradiated by iodine-125((125)I), the AFP antisense oligonucleotide labeled with various radioactivity dose (125)I was mixed with the AFP DNA in a simulated polymerase chain reaction temperature condition. After the mixtures were irradiated by the (125)I from 2 to 72 hours, the mutation of the biogenic conformation and functional groups of the irradiated DNA were investigated using laser Raman spectroscopy. The shifted peak and the decreased intensity of the characteristic Raman spectra were found, which demonstrated that the structure of the phosphodiester linkage was broke, the pyridine and purine bases in DNA emerged and damaged. The model of gene conformation changed from form B to form C spectrum after the nanometer-range irradiation with (125)I from 2 to 24 hours. The damage of local pyridine and purine bases gradually increased along with the accumulation of irradiation, and the bases and ribosome were finally dissociated and stacked.
Cancer Biotherapy & Radiopharmaceuticals 04/2013; 28(3):226-32. · 1.44 Impact Factor
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ABSTRACT: This retrospective study is designed to evaluate the diagnostic value of semi-quantitative parameters of salivary gland scintigraphy (SGS) in Sjögren's syndrome (SS) and explore the relationship between SGS parameters and salivary gland histopathologic manifestations. SGS and labial salivary gland biopsy were performed on 95 SS patients and 36 healthy age-matched and sex-matched volunteers. Uptake ratios at 15 min (UR(15)) and stimulated excretion fraction (EF) of each parotid and submandibular gland were calculated automatically from SGS; histopathologic grades were classified based on focal lymphocytic infiltration, and biopsy focus scores were calculated simultaneously. As a result, the UR(15) and EF of each salivary gland in SS patients were significantly lower than that in controls. Multivariate logistic regression equation was: logit p = 6.965 - 1.680 × UR(15) of left submandibular gland - 0.040 × EF of left parotid gland - 0.050 × EF of right submandibular gland. The receiver operating characteristic (ROC) curve validated high efficiency of this model with the area under curve of 0.911. And logistic regression analysis on combined original and validation cohorts confirmed its diagnostic value. Moreover, the EF of right parotid gland and both submandibular glands was correlated with histopathologic changes. Consequently, the semi-quantitative parameters of SGS may be helpful for diagnosing SS; UR(15) of left submandibular gland, EF of left parotid gland, and EF of right submandibular gland are more powerful predictor of SS. Besides, SGS parameters reflect the degree of salivary gland involvement, severe patients with low parameters would have high histopathologic grades and biopsy focus scores.
Clinical Rheumatology 09/2012; · 2.00 Impact Factor
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ABSTRACT: Reports of Tc macroaggregated albumin pulmonary perfusion scintigraphy for anomalous systemic arterial supply to normal basal segments of the left lower lobe of the lung are extremely rare. We performed scintigraphy in 2 patients with the disease to verify their diagnoses and guide the operation as both were initially misdiagnosed. Segmental radiotracer defects in the basal segments of the left lower lobe, which are also characteristic features of pulmonary embolism, were evident in both cases, as expected. Our report provides a complementary noninvasive diagnostic modality of the disease and helps prevent potential misinterpretation of Tc macroaggregated albumin scintigraphy.
Clinical nuclear medicine 04/2012; 37(4):377-9. · 3.92 Impact Factor
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ABSTRACT: Autism spectrum disorders (ASD), which include autism, asperger syndrome (AS) and pervasive developmental disorder-not otherwise specified (PDD-NOS), are devastating neurodevelopmental disorders of childhood resulting in deficits in social interaction, repetitive patterns of behaviors, and restricted interests and activities. Single photon emission computed tomography (SPECT) is a common technique used to measure regional cerebral blood flow (rCBF). Several studies have measured rCBF in children with ASD using SPECT, however, findings are discordant. In addition, the majority of subjects used in these studies were autistic. In this study, we aimed to investigate changes in rCBF in children with ASD using SPECT.
A Technetium-99m-ethyl cysteinate dimmer (⁹⁹m)Tc-ECD) brain SPECT study was performed on an ASD group consisting of 23 children (3 girls and 20 boys; mean age (7.2 ± 3.0) years) who were diagnosed according to Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV) criteria and an age-matched control group with 8 children (1 girl and 7 boys, mean age (5.5 ± 2.4) years). Image data were evaluated with Statistical Parametric Mapping, 5th version (SPM5). A Student's t test for unpaired data was used to compare rCBF and asymmetry in the autism and corresponding control group. The covariance analysis, taking age as covariance, was performed between the ASD and control group.
There was a significant reduction in rCBF in the bilateral frontal lobe (frontal poles, arcula frontal gyrus) and the bilateral basal ganglia in the autism group, and a reduction in the bilateral frontal, temporal, parietal, legumina nucleus and cerebellum in the AS group compared to the control. In addition, asymmetry of hemispheric hypoperfusion in the ASD group was observed. Inner-group comparison analysis revealed that rCBF decreased significantly in the bilateral frontal lobe (42.7%), basal nucleus (24.9%) and temporal lobe (22.8%) in the autism group, and in the bilateral cerebellum (22.8%), basal nucleus (19.3%) and right thalamencephalon (16.6%) in the AS group (P < 0.05).
The decrease in rCBF in ASD is a global event, which involves the bilateral frontal, temporal, limbic system and basal ganglias. Asymmetry of hemispheric hypoperfusion was more obvious in the AS group than the autism group, which indicates a different neurobiological mechanism from that of autism.
Chinese medical journal 05/2011; 124(9):1362-6. · 0.86 Impact Factor
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ABSTRACT: The technique of nanoparticle-mediated radionuclide-gene therapy has been developed to promote targeted gene therapy of liver
cancer. The AFP gene antisense oligonucleotide labeled with radioactive iodine-125 125I-AFPasON was encapsulated with chitosan nanoparticle. Then the nanoparticles were transfected into the Hepatic cell cancer
(HepG2 cell) to interfere its AFP gene expression. The transfection efficiency of the Nanoparticle-mediated radionuclide-gene,
the target site of the gene, the AFP gene expression in the cell and its DNA damage were investigated using the techniques
of nanoscale measurements such as confocal Raman scattering microscopy and multi-dimensional microscopy. All the parameters
were measured as functions of the radiation intensity, the time of the transfection, and the size of the nanoparticle. It
was found that Auger electron emitted from iodine-125 could damage the helical conformation and structure of DNA, and depress
the AFP gene expression. The DNA damage increased with the radiation intensity. The asON is an effective specific carrier
of the radionuclide iodine-125 into the target DNA. Mediated by chitosan nanoparticles, the effect of the 125IAFPasON on the DNA damage of the HepG2 cell can be enhanced to over twice.
KeywordsNanoparticle–Radionuclide–Antisense oligonucleotide–Raman spectra–Cancer
03/2010: pages 63-65;
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ABSTRACT: The purpose of this study was to investigate whether the findings of Tc-99m pertechnetate salivary gland scintigraphy (SGS) can predict the outcome of sialoendoscopic intervention in patients with obstructive submaxillaritis.
Eleven patients with obstructive submaxillaritis were prospectively recruited. All patients underwent sialoendoscopy. Tc-99m pertechnetate SGS was performed both before and after sialoendoscopy.
Sialoendoscopy was effective in the glands with tracer uptake (n = 9) demonstrated on SGS before the procedure, evidenced by not only relief of the symptoms but also improvement in excretion in post therapy salivary gland scintigraphy. In contrast, sialoendoscopy was ineffective in the glands without tracer uptake (n = 2) in pretherapy scans.
In patients with submaxillaritis, whether a sialoendoscopy should be performed depends on the results of salivary gland scintigraphy.
Clinical nuclear medicine 02/2010; 35(2):77-9. · 3.92 Impact Factor
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ABSTRACT: To date, there has been no report on the salivary gland functional outcomes after sialendoscopic surgery. The purpose of this study is to evaluate salivary gland functional recovery after sialendoscopic management of obstructive salivary gland disease.
A prospective, self-control study.
The present study was undertaken among patients scheduled for sialendoscopic surgery with unilateral salivary ductal obstructions. Glandular function was quantitatively assessed with the use of sialometry and scintigraphy preoperatively and at least 3 months postoperatively.
A consecutive series of 17 patients were followed for 14 +/- 8 months. Sialendoscopic procedures included removal of calculi in 15 cases and dilatation of stenosis in two cases. All patients were free of symptoms during follow-up. Before surgery, there was a significant decline in the resting and stimulated saliva flow rate, uptake index, and excretion fraction of the obstructive glands compared with the contralateral normal glands. Postoperatively, although the degrees of functional recovery varied in individuals, statistical analysis revealed that the glandular function increased significantly in the affected glands and had no differences when compared to the contralateral glands.
These data provide a unique functional assessment after sialendoscopic surgery. Our results demonstrate that sialendoscopy is an organ-preserving surgical approach which can achieve satisfactory functional recovery in the management of salivary ductal obstructions.
The Laryngoscope 03/2009; 119(4):646-52. · 1.75 Impact Factor
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ABSTRACT: The quantitative relationship between changes in portal vein pressure and the perfusion index was studied, and hepatocellular function was evaluated. A modified protocol of hepatobiliary dynamic scintigraphy with Tc-99m-labeled ethylene hepatobiliary iminodiacetic acid (EHIDA) was performed in 37 patients with hepatic cirrhosis and 12 healthy controls. In 18 patients, the portal vein pressure (PVP) was measured intra-operatively during the portal vein bypass procedure. The portal vein perfusion index (PVI) was obtained by a two-compartment model of hepatic perfusion. A three-compartment model was applied in the hepatocellular extracted and excreted time-radioactivity curves, and the hepatic functional index was calculated including the hepatic uptake index (UI), the mean residual index (MRI), the uptake speed index (UsI), the descent speed index (DsI), the peak uptake (PU), and the peak time (PT) of hepatic uptake. These function indices in the cirrhotic patients were compared to the indices in the healthy controls with a two-sided t-test; specifically, the PU (18.94+/-6.80 vs. 29.67+/-18.98, P<0.01), UI (0.84+/-5.99 vs. 18.41+/-13.87, P<0.05), and UsI (0.58+/-0.39 vs. 1.23+/-0.81, P<0.01) were slower in patients with hepatic cirrhosis compared with the healthy controls. The PT (16.83+/-4.89 vs. 10.95+/-2.79, P<0.001) and MRI (5.93+/-2.96 vs. 2.74+/-0.97, P<0.001) were more prolonged and the DsI (0.07+/-0.09 vs. 0.18+/-0.14, P<0.01) was less compared to controls. The PVI were significantly higher in cirrhotic patients than in controls (46.17+/-7.83 vs. 29.07+/-5.71, P<0.001), and the PVI correlated to the PVP (r=0.79, P<0.01). In conclusion, modified quantitative hepatobiliary dynamic scintigraphy can evaluate the changes of portal vein blood flow and monitor hepatocellular function, in which the portal vein blood flow can estimate portal vein pressure.
Computerized medical imaging and graphics: the official journal of the Computerized Medical Imaging Society 01/2009; 33(3):182-6. · 1.04 Impact Factor
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ABSTRACT: Ventricular stroke work (SW) is one of the best indices to evaluate ventricular function, however, the SW monitoring mainly depends on the invasive method with the artery catheter. In this paper, our aim is to develop a new semi-quantitative non-invasive method for evaluating ventricular SW.
The multiple gated cardiac blood pool imaging was done in 25 patients with coronary artery disease and 12 normal controls. A new parameter, the relative stroke work (RSW) of left ventricle, was calculated using an equation derived from the principle of hydrodynamics. The left ventricular SW was analyzed by stroke volume (SV) and mean arterial pressure. Ejected fraction (EF), peak ejected rate (PER) and peak filling rate (PFR) were gotten with the routine software in imaging device.
The left ventricular RSW was linearly correlated with the SW. The RSW was related to the SV, EF, PER and PFR of the left ventricle. The RSW had regressive relation with SV and PER. The RSW in patients, same as SW, SV, EF, PFR and PER, was noticeably lower than that in normal controls, P<0.01.
The RSW is a potential and valuable clinical index for evaluation of the ventricular function.
Clinical Physiology and Functional Imaging 01/2009; 29(2):95-9. · 1.33 Impact Factor
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ABSTRACT: To evaluate the influence of Chinese drug Dahuang Zhechong pill on the hepatocellular function.
Thirty-seven patients with hepatocirrhosis and twelve normal controls were performed the hepatobiliary scintgraphy with Tc-99m labeled ethylene hepatobiliary iminodiacetic acid (99 mTc-EHIDA), and the biochemical examination of hepatic function. There was 19 cases repeated the imaging after 6 months treated with chineses drug. By the three compartmental model configurations, the function parameters of hepatocellular extraction and excretion were calculated.
In the hepatocirrhosis groups, the hepatocellular uptake peak time and mean residence index were higher than those in normal controls (P < 0.01). Compared to normal controls, the uptake index, uptake speed index and descendent speed index were decreased markedly (P < 0.05). After treatment for 6 months with Chinese drug, the level of serum transaminase, globulin and bilirubin was lower than that before treatment. The uptake peak time and mean residence index decreased notably after treatment for 6 months (P < 0.01), and the uptake index increased, (P < 0.05).
Chinese drug Dahuang Zhechong pill may improve the hepatocellular function and liver function status in patients with hepatocirrhosis.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 04/2008; 33(5):564-6.
