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ABSTRACT: Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (>106 cpm/μg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.
Journal of Immunological Methods 02/1979; · 2.23 Impact Factor