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ABSTRACT: The prognostic relevance of World Health Organization (WHO) subtypes within type B thymomas is still controversial. Understanding of the molecular characteristics of the different histologic types of thymomas will provide meaningful information for diagnosis and therapeutic management in type B thymoma.
Proteins extracted from twelve type B thymoma tissue specimens (six type B1 and six type B2) were analyzed by two-dimensional electrophoresis (2-DE) coupled with MALDI-TOF-MS. Differentially expressed proteins were then assayed in sixty-nine type B thymoma tissues (including B1, B2 and B3) by tissue array analysis with immunohistochemistry staining. The relationship of their expression with clinicopathological parameters, such as tumor stage or WHO classification, was estimated by Spearman's Rank Correlation Test.
Sixteen differentially expressed proteins between type B1 and B2 thymoma tissues were identified. The differential levels of ezrin and glutathione S-transferase pi (GSTP1) were validated using immunohistochemistry staining. A statistically significant difference was observed in the positive rate of ezrin expression between type B1 thymoma and type B3 thymoma (Z = -2.963, P < 0.01). Ezrin showed a tendency to be expressed in higher classification tumors from type B1 to B3. A statistical analysis demonstrated that type B2 and B3 tumors had significantly higher positive expression of GSTP1 than the B1 group (type B2 vs. B1: Z = -2.582, P = 0.01; type B3 vs. B1: Z = -4.012, P ≤ 0.001). The results also showed a strong correlation between GSTP1 and WHO type staging of B1 to B3 tumors (Spearman's correlation coefficient: 0.633, P ≤ 0.001). Statistical analysis showed that there was close correlation between GSTP1 and ezrin expression with the clinical stage (Spearman's correlation coefficients, ezrin: 0.481, P < 0.05; GSTP1: 0.484, P < 0.01).
Differentially expressed proteins between type B1 and B2 thymoma tissues were analyzed by comparative proteomic analysis. The techniques of proteomic analysis and tissue array provide a potential tool for screening of key molecules in type B thymoma histological sub-classifications. The statistical analysis of ezrin and GSTP1 expression by immunohistochemistry, especially GSTP1, may be a useful approach for type B thymoma classification.
Chinese medical journal 08/2012; 125(16):2811-8. · 0.86 Impact Factor
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ABSTRACT: Due to its great importance in fundamental research and practical applications, tailored synthesis of anatase TiO2 dominated with highly energetic {001} facets has been extensively studied during the past few years. However, clean (001) surface of anatase TiO2 has been evidenced to be unstable and usually tends to reconstruct under ultrahigh-vacuum conditions. Thus, the stability of surface structure under other ambient conditions might be one of the most critical issues for anatase TiO2 with exposed high-reactive {001} facets. In this study, the hydrothermal stability of {001} faceted anatase TiO2 was systematically investigated by using single-crystalline anatase TiO2 nanosheets with 80% {001} facets as a model starting material. Under hydrothermal conditions (200 °C in deionized water), anatase TiO2 nanosheets can grow into larger single crystals with a truncated bipyramidal shape through an oriented attachment process along the [001] crystallographic direction, driven by the minimization of surface energy. Furthermore, the coarsening behavior and growth mechanism were discussed with the assistance of standard and high-resolution transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and inductively coupled plasma optical emission spectrometry, as well as theoretical calculations. A modified kinetic model was also developed to elucidate the asymptotic growth of anatase TiO2 nanosheets via oriented attachment mechanism. In addition, pH value and the solvents adopted during the treatments were revealed to have significant influence on the oriented attachment-based crystal growth due to suppression of hydrolysis of Ti–F groups on the surface. For example, the anatase TiO2 nanosheets remained their original morphology unchanged when ethanol, propanol, butanol, or 1.5 M HCl aqueous solution was used as reaction medium.Keywords: titanium dioxide; nanosheets; oriented attachment; hydrothermal stability; high-reactive facets
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Chemistry 06/2011; 17(24):6615-9. · 5.93 Impact Factor
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ABSTRACT: In this work, we report a facile top-down approach to fabricate uniform single-crystal α-Fe(2)O(3) nanodiscs via selective oxalic acid etching. Phosphate ions are employed as a capping agent to control the etching to along the [001] direction. We also show that α-Fe(2)O(3) melon-like microparticles with contrasting textural properties can be generated using the same approach. The etched particles exhibit a much larger total pore volume and average pore size compared to the pristine ones, thus serving as the possible origin for their greatly enhanced capacity retention when tested as potential anode materials for lithium-ion batteries.
Journal of the American Chemical Society 09/2010; 132(38):13162-4. · 9.91 Impact Factor
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ABSTRACT: Anatase TiO(2) having different percentages of (001)/(101) surface demonstrated different behaviors for Li(+) ions insertion and much enhanced rate performance of Li-ion batteries.
Chemical Communications 09/2010; 46(33):6129-31. · 6.17 Impact Factor
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ABSTRACT: Hydroxyl radical molecular beam was generated by DC pulsed high voltage discharge. The rotational resolution excitation fluorescence spectra of the OH A 2sigma+ -X 2pi (1, 0) band were observed by using second harmonious output of a nanosecond dye laser (around 282 nm). The rotational temperature of the (1, 0) band was determined to be (30 +/- 1) K based on the analysis of the intensity distribution of the OH spectra. Moreover, the fluorescence decay curves of the (1, 1) band and the (0, 0) band and were obtained and the fluorescence lifetimes of A 2sigma+ (v' = 1) state and the X 2pi (v" = 0) state were fitted to be (637 +/- 16) and (675 +/- 13) ns, respectively.
Guang pu xue yu guang pu fen xi = Guang pu 07/2010; 30(7):1738-42. · 0.84 Impact Factor
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ABSTRACT: Paclitaxel is used as the first-line chemotherapy for Non-Small Cell Lung Cancer (NSCLC), but acquired resistance becomes a critical problem. Several mechanisms have been proposed in paclitaxel resistance, but they are not sufficient to exhaustively explain this resistance emergence. To better investigate molecular resistance mechanisms, a comparative proteomic approach was carried out to identify differentially expressed proteins between human lung adenocarcinoma A549 cell line (paclitaxel sensitive) and A549-Taxol cell line (acquired resistant).
A paclitaxel-resistant subline (A549-Taxol) derived from the parental-sensitive cell line A549 was established by stepwise selection by paclitaxel. Total proteins in the two cell lines were separated by fluorescent differential gel electrophoresis (DIGE). Image analysis was carried out with the DeCyder 2D 6.5 software. Proteins associated with chemoresistance process were identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS). Some key molecules were valuated by Western blot.
Thirty proteins were identified and grouped into eight main functional classes according to the biological processes in which they are likely to participate, i.e. signal transduction, cytoskeleton, redox reaction, energy and metabolism, and so on. Alterations of these processes might be involved in paclitaxel resistance. Most of the proteins showed mitochondrial and cytoplasm location. The up-regulation of CK8, CK18, ALDH1, CAST and ANX I in A549-Taxol cell line was verified by Western blot, in coincidence with the data obtained from proteomic analysis.
For the first time, differentially expressed proteins between paclitaxel-sensitive cell line and paclitaxel-resistant one were explored by comparative proteomic approach in human lung adenocarcinoma. It may be useful for further studying of resistance mechanisms and screening of resistance biomarkers, so as to develop tailored therapeutic strategies.
Journal of Cancer Research and Clinical Oncology 05/2010; 137(3):521-32. · 2.56 Impact Factor
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ABSTRACT: To study the effects and possible mechanisms of the protein kinase C (PKC) inhibitor chelerythrine chloride (CH), combined with cisplatin (DDP) on human non-small cell lung cancer.
The effect of CH, DDP and the combination on proliferation and apoptosis of human lung cancer cell line A549 were evaluated by MTT assay and flow cytometry respectively. The inhibitory effects of CH and DDP on neoplasia were verified on subcutaneous implanted tumor of nude mice. Implanted tumor models were constructed in nude mice using human lung adenocarcinoma cell line A549. Twenty-four BALB/c nude mice with implanted tumors were divided into 4 groups randomly: group CH, group DDP, group CH + DDP, and normal saline group (group NS), each with 5 mice. CH, DDP or NS were intraperitoneally injected into nude mice for 3 weeks (DDP or NS was injected once a week, CH was injected twice a week).
CH inhibited A549 cell proliferation in a concentration-dependent pattern. When CH and DDP were combined, the inhibitory effect was enhanced in a synergistic or additive pattern. Both CH and DDP significantly increased the apoptosis of A549 cells, and this action was remarkably increased when DDP was combined with CH. CH and DDP inhibited the growth of subcutaneous implanted tumor in nude mice and the inhibitory rate of group CH + DDP (80.5%) was significantly higher than that of group CH (72.4%) or group DDP (64.3%) (t = 11.34, P < 0.01).
CH combined with DDP shows significantly synergistic anti-tumor effects on non-small cell lung cancer cell line A549 and subcutaneous implanted tumor in nude mice, possibly by enhancement of growth inhibition and apoptosis induction on tumor cells.
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 04/2010; 33(4):284-8.
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ABSTRACT: Brassinosteroids (BRs) are perceived by transmembrane receptors and play vital roles in plant growth and development, as well as cell in responses to environmental stimuli. The transmembrane receptor BRI1 can directly bind to brassinolide (BL), and BAK1 interacts with BRI1 to enhance the BRI1-mediated BR signaling. Our previous studies indicated that a membrane steroid-binding protein 1 (MSBP1) could bind to BL in vitro and is negatively involved in BR signaling. To further elucidate the underlying mechanism, we here show that MSBP1 specifically interacts with the extracellular domain of BAK1 in vivo in a BL-independent manner. Suppressed cell expansion and BR responses by increased expression of MSBP1 can be recovered by overexpressing BAK1 or its intracellular kinase domain, suggesting that MSBP1 may suppress BR signaling through interacting with BAK1. Subcellular localization studies revealed that both MSBP1 and BAK1 are localized to plasma membrane and endocytic vesicles and MSBP1 accelerates BAK1 endocytosis, which results in suppressed BR signaling by shifting the equilibrium of BAK1 toward endosomes. Indeed, enhanced MSBP1 expression reduces the interaction between BRI1 and BAK1 in vivo, demonstrating that MSBP1 acts as a negative factor at an early step of the BR signaling pathway.
Cell Research 07/2009; 19(7):864-76. · 8.19 Impact Factor
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ABSTRACT: A graphically oriented interactive program for assignments of rotationally resolved molecular spectra has been devised. The program functions by grouping spectral lines in term of the second difference principle, and graphing spectral intensity versus frequency in a bar graph of the selected groups, distinguished by color and/or line-type. This allows for easy detection of regular patterns buried in the observed spectrum. Furthermore, it includes a Loomis–Wood view for assisting in spectral assignments. As an example, the program was applied in assigning the molecular spectrum of the production in the discharge of PCl3 buffered by helium gas, which may belong to several species. The results suggest that the program is highly efficient and quite useful in the assignment and the analysis of molecular spectra, especially those of symmetric top, slightly asymmetric top and linear molecules. The accuracy and efficiency of this program will likely ensure its wide application in the processing of molecular spectra.
Chinese Journal of Chemical Physics 06/2009; 19(1):39. · 0.47 Impact Factor
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ABSTRACT: The intermediate decomposition products of methanol (CH3OH) in an AC discharge were diagnosticated via concentration modulation spectroscopy. Several main vibrational bands of CO Angström beta 1 sigma+ -alpha 1pi system, CH alpha 2 delta-X2pi system at 430 nm and beta 2 sigma- -X2pi at 390 nm, as well as CHO (329.82 nm), CH2O (369.8 nm), CH3O (347.8 nm) and Balmer series spectra of H atom were identified in the region between 300 and 670 nm. Furthermore, the dependences of the emission spectral intensities of the intermediate decomposition products on the discharge voltage and parent gas pressure were investigated in detail. The experiments indicated that the relative population ratio of CO (beta 1 sigma+) and H increases with increasing discharge voltage more quickly than that of CH (alpha 2 delta). Several possible reaction passages were given and discussed. Additionally, the vibrational and rotational temperatures of CH (alpha 2 delta) were determined to be about 4200 and 1100 K respectively, and the vibrational temperatures of CO (beta 1 sigma+) were determined to be about 2500 K by analyzing the intensity distribution using LIFEBASE computer program. The decomposition mechanism of methanol in the discharge plasma was discussed as well.
Guang pu xue yu guang pu fen xi = Guang pu 10/2008; 28(9):1983-6. · 0.84 Impact Factor
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ABSTRACT: N2 plasma jet was generated from a nozzle using an AC discharge at about 15 kV. The emission spectra were obtained by using the concentration modulation spectroscopic technique. The dependence of spectral signal on the discharge current was discussed. It was found that the vibrational temperature decreased first and then increased downstream from the nozzle by measuring the emission spectra from different axial positions in the jet. A phenomenological model was presented to explain the vibrational temperature variation with different axial position z. The N2+/N2 ratio varied along the axis and the property of discharge in the experimental system was studied.
Guang pu xue yu guang pu fen xi = Guang pu 10/2007; 27(9):1680-4. · 0.84 Impact Factor
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ABSTRACT: An experimental apparatus using an AC discharge was developed for producing molecular ion beam. The emission spectral intensity of N2+ is stronger than that of N2, and a very high ratio of N2+ (B)/N2 (C) up to 6:1, was achieved in N2 discharge. The effects and processes of N2 discharge, and the concentration modulation spectra were studied. The vibrational temperature and rotational temperature of N2+ molecular ions beam were calculated to be 3 310 and 282 K respectively from the emission spectra.
Guang pu xue yu guang pu fen xi = Guang pu 10/2006; 26(9):1594-7. · 0.84 Impact Factor
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ABSTRACT: A new method for preparing SERS-active substrate was reported. After being exposed, developed and fixed, the commercially available holographic plate can be used to generate attractive SERS-active silver surface. The obtained silver film was characterized by UV-Vis spectroscopy and scanning electron microscope (SEM) observation. The absorption spectrum locates at around 420 nm with a narrow half-width due to the surface plasmon resonance, which implies that there are silver particles on the prepared substrate with uniform sizes. And the silver particles distribute uniformly anywhere on the holographic plate with a diameter of about 100 nm by SEM observation. Furthermore, the measurements of the SERS spectrum of crystal violet indicate that such substrate has the features of satisfactory reproducibility, long term stability and high SERS activity. The prepared silver film keeps high SERS activity even if it was stored for several months in the air at room temperature.
Guang pu xue yu guang pu fen xi = Guang pu 01/2006; 25(12):2009-11. · 0.84 Impact Factor
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ABSTRACT: The molecular cation CO+ is a very important transient molecular radical, and the spectra of the ion has been investigated by numerous researchers. Optical-heterodyne-magnetic-rotation-enhanced velocity modulation spectroscopy(OH-MR-VMS) was employed to measure the comet-tail system (A2 pi(i)-X2 sigma+) of 12C16O+ molecular ion. The vibrational rotation absorption spectra wereobserved in the near-infrared region, including three bands of (0, 3), (1, 4) and (3, 6). A set of precise molecular constants for the A2 pi(i) (v' = 0, 1, 3) were determined using the least-squares fit. This experiment has further proved that OH-MR-VMS is very valuable in measuring absorption spectra of molecular ions.
Guang pu xue yu guang pu fen xi = Guang pu 09/2005; 25(8):1250-2. · 0.84 Impact Factor
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ABSTRACT: The CN radical was produced by Penning AC discharge of the mixture of trace acetonitrile (36 Pa) and helium (660 Pa) and the ro-vibrational spectra were measured by optical heterodyne magnetic rotation enhanced concentration modulation spectroscopy in the range of 16 850-17 480 cm(-1). 138 lines and 118 lines were assigned to the (6, 1) and (7, 2) bands in red system A 2IIi-X 2sigma+ of CN respectively, while others were due to (8, 3) band in CN red system and C2 Swan band system. More accurate molecular constants of A 2IIi (v = 6, 7) state and perturbation parameters zeta and eta between A and X states were derived based on nonlinear least-square fitting, individually, by diagonalizing the effective Hamiltonian matrix involving the perturbation elements of two electronical states and the standard deviation of fitting was less than the experimental uncertainty 0.007 cm(-1), which implied the accuracy of our results.
Guang pu xue yu guang pu fen xi = Guang pu 02/2005; 25(1):65-8. · 0.84 Impact Factor
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ABSTRACT: A putative Membrane Steroid Binding Protein (designated MSBP1) was identified and functionally characterized as a negative regulator of cell elongation in Arabidopsis thaliana. The MSBP1 gene encodes a 220-amino acid protein that can bind to progesterone, 5-dihydrotestosterone, 24-epi-brassinolide (24-eBL), and stigmasterol with different affinities in vitro. Transgenic plants overexpressing MSBP1 showed short hypocotyl phenotype and increased steroid binding capacity in membrane fractions, whereas antisense MSBP1 transgenic plants showed long hypocotyl phenotypes and reduced steroid binding capacity, indicating that MSBP1 negatively regulates hypocotyl elongation. The reduced cell elongation of MSBP1-overexpressing plants was correlated with altered expression of genes involved in cell elongation, such as expansins and extensins, indicating that enhanced MSBP1 affected a regulatory pathway for cell elongation. Suppression or overexpression of MSBP1 resulted in enhanced or reduced sensitivities, respectively, to exogenous progesterone and 24-eBL, suggesting a negative role of MSBP1 in steroid signaling. Expression of MSBP1 in hypocotyls is suppressed by darkness and activated by light, suggesting that MSBP1, as a negative regulator of cell elongation, plays a role in plant photomorphogenesis. This study demonstrates the functional roles of a steroid binding protein in growth regulation in higher plants.
The Plant Cell 02/2005; 17(1):116-31. · 8.99 Impact Factor
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ABSTRACT: A new method for the calibration of wavelength in a measurement of high resolution molecular absorption spectra by referring the standard spectrum of iodine molecule is presented in this paper. Segment offset is employed in the calibration of wavelength, while the data smoothing, baseline determining and peak fitting are applied to preprocess the spectral data. A visual software is provided for automatically calibrating wavelength with a friendly style of mutual dialogue between human and computer. The software has been used to calibrate the absorption spectra of N2+ molecular ion in the visible region 16,422-17,896 cm-1, the absolute accuracy is about 0.006 cm-1.
Guang pu xue yu guang pu fen xi = Guang pu 09/2002; 22(4):569-72. · 0.84 Impact Factor
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ABSTRACT: The ro-vibrational spectrum of the weak (4, 6) hot band in the comet-tail (A2Πi–X2Σ+) system of CO+ is observed by employing optical heterodyne and magnetic rotation enhanced velocity modulation spectroscopy. One hundred and twenty-four spectral lines are assigned to this band; thus, precise molecular constants of the levels involved are obtained from a weighted nonlinear least-squares fitting procedure combining with our previous spectrum of the (3, 6) band.Research highlights► About 1/60 weak band (4, 6) in the comet-tail (A2Πi–X2Σ+) system of CO+ is directly observed. ► 124 spectral lines are assigned to the (4, 6) band. ► Precise molecular constants of the levels involved are obtained. ► The improvement of the molecular constants is up to one order.
Journal of Quantitative Spectroscopy and Radiative Transfer 112(6):1005-1009. · 3.19 Impact Factor
