I N Lutsenko

Publications (16)7.07 Total impact

• Article: [Ten-year outcomes of lymphogranulomatosis treatment according to the protocol MOPP-ABVD+radiotherapy].

  A V Pivnik, N A Rastrigin, T N Moiseeva, I N Lutsenko, R G Dudarova, V S Shavlokhov, I V Efimov, S V Kozhurin, I V Shitareva, E G Gemdzhian, N N Tsyba, L Iu Kolosova, A L Melikian, N I Skidan, G A Pan'shin, V M Sotnikov, P V Dotsenko, A V Nechistiuk
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  ABSTRACT: To analyse overall recurrence-free survival of lymphogranulomatosis (LGM) patients given polychemotherapy (PCT) MOPP (mustargen-caryolisin, vincristine, natulan, prednisolone) - ABVD (adriamycin, bleomycin, vinblastin, dacarbasin) in combination with radiotherapy (RT) for 10 years. The trial included 211 LGM patients admitted to Hematological Research Center in 1990-1996 from other hospitals without random selection. The patients were examined by the standard program including biopsy of the affected organ or lymph node, bilateral trephine biopsy. Splenectomy was performed in 17 patients, 83 patients received PCT in other hospitals, 128 untreated patients received MOPP-ABVD therapy (3 courses of MOPP and 3 courses of ABVD). Forty one patients had defects in PCT, 16 of them rejected PCT and RT. The latter was performed 4 weeks after the 6th course, contraceptives were not prescribed to women. At LGM stage II-III RT was performed by the subradical program (no radiation to ilioinguinal lymph nodes) in doses 40-44 Gy on the foci and 32-36 Gy preventively, on massive and residual foci after PCT - 5-10 Gy additionally. Ten-year overall and recurrence-free survival in the untreated group reached 83 and 80%, respectively, for pretreated patients - 46 and 36%, respectively. Causes of death of 26 patients were LGM progression, infection (tuberculosis, as a rule), secondary tumors and acute myeloblastic leukemia (AML). After remission 25 women gave birth to a healthy child and 12 healthy children were born to 9 males. MOPP-ABVD plus radiotherapy program according to subradical and radical variants was in the past effective but invalidating rescue therapy. Present-day programs consider the histological variant, stage and prognostic factors allowing an individual therapeutic approach with step-by-step reduction of RT in the treatment of LGM patients. Involvement of the bone marrow in primary patients had no influence on the treatment results. This refers this affection not to a generalized stage IV, but to stage III along with involvement of the lymph nodes and the spleen.
  Terapevticheskii arkhiv 02/2006; 78(8):57-62. · 0.14 Impact Factor
• Article: [Clinicomorphological characteristics of Sezary's disease and mycosis fungoides].

  Iu E Vinogradova, E A Giliazitdinova, I A Zamulaeva, E E Zybunova, I B Kaplanskaia, S K Kravchenko, A M Kremenetskaia, I N Lutsenko, R S Samoĭlova, E N Selivanova, Iu V Sidorova, N E Shklovskiĭ-Kord
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  ABSTRACT: To try a combined approach to the study of clinicomorphological and immunophenotypical characteristics of primary cutaneous T-cell lymphomas. Clinical, histological, genotypic and immunophenotypical parameters were studied in 7 patients (4 male and 3 female, mean age 53.1 +/- 7.8%) with Sezary's disease (SD) and 10 patients (6 male, 4 female, mean age 54.0 +/- 4.0 years) with mycosis fungoides (MF) treated in Hematological Research Center in 1998-2004. Six of seven SD patients had SD stage IV with leukemization, Sezary's cells were found in peripheral blood. Bone marrow and lymph nodes involvement was observed in 5 patients. Morphological signs of transformation into lymphosarcoma were detected in three patients. Skin samples of all the patients showed epidermotropism with lymphoid infiltration of the derma and skin appendages. All the patients had clonal rearrangement of T-cell receptor by gamma-chain. Immunophenotyping (IPT) detected T-cell markers CD45RO, CD43, CD3, CD4 on lymphoid cells. IPT of peripheral blood lymphoid cells was typical for SD in 3 patients. Low density of CD4 and CD2, CD4 and CD5, the presence of CD7 were registered in 1 patient each. The disease history was 3.4 +/- 0.7 years. A lethal outcome was related with septic complications after polychemotherapy. MF history in 10 patients was 10.9 +/- 2.1 years. Stages III and IV were diagnosed in 2 of 10 patients. All the patients had typical pathohistological changes. Polymerase chain reaction test detected clone by rearrangement of gamma-chain of T-cell receptor. In 2 patients IPT detected CD4 absence in the presence of CD8 and CD7. The aberrant clone typical for NK-cells was detected in one case. Two patients died of the disease progression after 7 and 20 years of MF. Multiple tests help early diagnosis and conduction of optimal therapy for cutaneous T-cell lymphomas.
  Terapevticheskii arkhiv 02/2005; 77(10):61-5. · 0.14 Impact Factor
• Article: [The first case of Hb Camden carriage in Russia].

  E A Aseeva, I N Lutsenko, A V Pivnik, V A Spivak, L S Beliaeva, A M Kremenetskaia, V Saad
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  ABSTRACT: Structural hemoglobinopathy due to carriage of Hb Camden was diagnosed in a male patient and his mother. Anomalous Hb was detected after a detailed analysis of the clinical evidence, blood smears microscopy, marrow examination, data obtained by a complex of methods to identify unstable Hb. It was defined as Hb Camden beta 131 GLN-->GLU. The carriage of this variant brings no clinical manifestations, but leads to impaired red cells morphology, decline of their life span, enhanced hemolysis. These properties are typical for mutant Hb lacking stability. Hb Camden carriage is considered as a compensatory hemolytic process.
  Terapevticheskii arkhiv 01/1993; 65(7):49-51. · 0.14 Impact Factor
• Article: [Distribution of abnormal hemoglobins S and C in the Republic of Guinea].

  V A Spivak, A Sou, I N Lutsenko
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  ABSTRACT: Distribution of the HbS and HbC in the Guinean Republic was determined by the analysis of the excerption of 2213 inhabitants representing different ethnic groups of the country. It was found that the mean frequency of the HbS heterozygotes is 21.2 +/- 0.9% and of the HbC heterozygotes is 2.6 +/- 0.3%. Among major nationalities of the country the frequencies of the HbS heterozygotes make up 21.0 +/- 1.9% for the Fulbe, 22.2 +/- 1.6% for the Malinke and 26.5 +/- 1.6% for the Susu and frequencies of the HbC heterozygotes make up 3.0 +/- 0.8% for the Fulbe, 2.5 +/- 0.6% for the Malinke and 1.6 +/- 0.4% for the Susu. Relative viability of the HbS and HbC carriers for major Guinean nationalities is estimated. The relative fitnesses account is 1.05-1.13 for HbC heterozygotes and 1.07-1.16 for HbC heterozygotes.
  Genetika 09/1992; 28(8):159-65. · 0.44 Impact Factor
• Article: [Congenital hemolytic anemia caused by the carriage of hemoglobin Bristol beta 67 Bal-->Asp].

  E A Aseeva, I N Lutsenko, A V Pivnik, V A Spivak, L S Beliaeva, N M Zhestkova
  Terapevticheskii arkhiv 02/1992; 64(7):78-81. · 0.14 Impact Factor
• Article: [Abnormal hemoglobins Sbeta6Glu->Val and Cbeta6Glu->Lys in the Republic of Guinea].

  A Sou, E A Aseeva, I N Lutsenko, V A Spivak
  Gematologiia i transfuziologiia 02/1991; 36(1):35-7. · 0.09 Impact Factor
• Article: [Rapid analysis of HbC beta 6Glu----Lys].

  V A Spivak, I N Lutsenko, E A Aseeva, A Sou
  Gematologiia i transfuziologiia 04/1989; 34(3):59-60. · 0.09 Impact Factor
• Article: Hemoglobin Siriraj found in the Hungarian population.

  J Földi, M Horányi, J G Szelényi, S R Hollán, E A Aseeva, I N Lutsenko, V A Spivak, O Tóth, B V Rozynov
  Hemoglobin 02/1989; 13(2):177-80. · 1.30 Impact Factor
• Article: [A new strategy for structural identification of abnormal human hemoglobins].

  V A Spivak, I N Lutsenko, E A Aseeva
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  ABSTRACT: A new strategy for structural identification of abnormal human hemoglobins is proposed. It is based on micropreparative modification of electrophoretic separation of globins on Cellogel strips with subsequent quantitative isolation of a pure, desalted globin chain, in a form suitable for its subsequent structural investigation. Among the major advantages of the new strategy age possibility to use small blood samples (0.1-0.2 ml), short analysis time, relative simplicity and low cost.
  Molekuliarnaia genetika, mikrobiologiia i virusologiia 09/1988;
• Article: Micropreparative electrophoresis of globin chains on cellulose acetate film in structural identification of abnormal human hemoglobins.

  V A Spivak, I N Lutsenko
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  ABSTRACT: A simple and rapid micropreparative method for isolating 5 to 30 nmol of globin chain, followed by structural identification of abnormal human hemoglobins, is described. The method is based on the electrophoretic separation of globins on ordinary Cellogel films under denaturating conditions with subsequent cutting out of the protein zones and solubilization of Cellogel and the electrophoretic buffer components in a specially selected solvent in which the globin chain undergoes quantitative precipitation. The method makes it possible to simplify and speed up the structural identification of commonly occurring abnormal hemoglobins. The advantages and limitations of the method are discussed along with its potential uses in structural protein chemistry.
  Analytical Biochemistry 06/1988; 171(1):108-12. · 3.00 Impact Factor
• Article: [Micropreparative thin-layer peptide maps in the molecular diagnosis of abnormal human hemoglobins].

  V A Spivak, E A Aseeva, I N Lutsenko
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  ABSTRACT: General factors determining the possibility of application of peptide maps in thin layer of microcrystalline cellulose as a micropreparative method in molecular diagnostics of abnormal hemoglobins were studied. The effects of absorbtional capacity of cellulose and amino acid impurities in it, choice of eluent and elution technique, peptide structure and extent of its modification in staining as well as completeness and specificity of globin chain enzymatic digestion on peptides extraction from thin layer were analysed. The results of structural identification of Hb D Punjab beta 121 Glu----Gln at a Cypriot; Hb O Arab; beta 121 Glu----Lys at a Bulgarian woman, living in Kalinin region (the second case in the USSR); Hb M Saskatoon beta 63 His----Tyr at a woman from Georgia (the second case in the USSR); Hb Buenos Aires beta 85 Phe----Ser at a Russian girl (the first case in the USSR and the third case in the world); Hb Dagestan alpha 60 Lys----Glu at two members of a Lesgin family from Dagestan; Hb Agenogi beta 90 Glu----Lys at a Hungarian woman; Hb Setif alpha 94 Asp----Tyr at three patients from Cyprus and Hb Detroit beta 95 Lys----Asn at an Azerbaijanian woman (the first case in the USSR and the second case in the world) are presented.
  Molekuliarnaia genetika, mikrobiologiia i virusologiia 05/1988;
• Article: [2 related families carrying HbC beta 6 Glu--Lys in the Azerbaijan population].

  R Sh Rustamov, E G Gadzhiev, I N Lutsenko, E A Aseeva, V A Spivak
  Gematologiia i transfuziologiia 02/1987; 32(1):55-7. · 0.09 Impact Factor
• Article: [Proteolysis of globin chains on acetylcellulose film in the molecular diagnosis of abnormal hemoglobins].

  I N Lutsenko, V A Spivak, Iu N Tokarev
  Gematologiia i transfuziologiia 03/1985; 30(2):47-50. · 0.09 Impact Factor
• Article: A new abnormal hemoglobin: Hb Mozhaisk beta 92(F8)His leads to Arg.

  V A Spivak, T P Molchanova, Postnikov YuV, E A Aseeva, I N Lutsenko, Tokarev YuN
  Hemoglobin 02/1982; 6(2):169-81. · 1.30 Impact Factor
• Article: [Oxygen transport and the acid-base equilibrium in endemic hereditary erythrocytosis].

  M G Dmitrieva, A S Poliakova, A P Andreeva, E G Kazanets, I N Lutsenko
  Problemy gematologii i perelivaniia krovi 12/1979; 24(11):8-11.
• Article: [Hemoglobin oxygen-transport activity in lymphosarcomas].

  I V Karpova, M G Dmitrieva, A V Pivnik, E E Zybunova, I N Lutsenko
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  ABSTRACT: To evaluate oxygen transport function of Hb in lymphoid sarcomas, 15 patients aged 16-63 were examined for Hb oxygen capacity, concentrations of fetal Hb and creatinin, basic ligands H+, CO2, 2,3-diphosphoglycerate (2,3-DPG). Oxy-Hb dissociation curves were plotted. The patients' blood contained HbF in elevated quantities, whereas red cells had high creatinin. This emphasizes the role of hemolysis in genesis of anemia in lymphosarcomas. 70% of the examinees retained normal Hb affinity to oxygen in vivo, in 30% this affinity exceeded the standard. Compensatory mechanism responsible for low Hb affinity to oxygen in this disease fails. Therefore lymphosarcoma patients with anemia need early replacement hemotransfusions.
  Gematologiia i transfuziologiia 40(6):10-4. · 0.09 Impact Factor