Hui Jiang

Southeast University (China), Nan-ching-hsü, Jiangxi Sheng, China

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Publications (63)245.07 Total impact

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    ABSTRACT: A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M(-1) cm(-2), n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.
    Analytical Chemistry 09/2014; · 5.82 Impact Factor
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    ABSTRACT: Fluorescent gold/silver nanoclusters templated by DNA or oligonucleotides have been widely reported, since DNA or oligonucleotides could be designed to position a few metal ions at close proximity prior to their reduction, but nucleoside-templated synthesis is more challenging. In this work, a novel type of strategy taking cytidine (C) as template to rapid synthesis of fluorescent, water-soluble gold and silver nanoclusters (C-AuAg NCs) has been developed. The as-prepared C-AuAg NCs have been characterized by UV-Vis absorption spectroscopy, fluorescence, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR) and inductively coupled plasma-mass spectroscopy (ICP-MS). The characterizations demonstrate that C-AuAg NCs with a diameter of 1.50 ± 0.31 nm, a quantum yield ~ 9% and an average lifetime ~ 6.07 µs possess prominent fluorescence properties, good dispersibility and easy water solubility, indicating the promising application in bioanalysis and biomedical diagnosis. Furthermore, this strategy by rapid producing of highly fluorescent nanoclusters could be explored for the possible recognition of some disease related changes in blood serum. This raises the possibility of their promising application in bioanalysis and biomedical diagnosis.
    Langmuir : the ACS journal of surfaces and colloids. 08/2014;
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    ABSTRACT: Simultaneous and multisite tumor rapid-target bioimaging has been realized in this contribution through in vivo biosynthesis of fluorescent gold nanoclusters (GNCs). The selectively biosynthesized fluorescent GNCs in cancer cells or tumor tissues by systemic bio-administration of gold precursors via tail vein injection in tumor bearing mice were found to exhibit a highly efficient tumor targeting effect. Intracellular fluorescence studies demonstrate that in vivo biosynthesized GNCs from cancer cells could efficiently label and image target cells with bright photostable fluorescence, which could be readily exploited for the rapid imaging in vivo of the biodistribution of GNCs in mice and thus efficiently determine the precise target sites of fluorescent GNCs specifically biosynthesized in tumor tissues with high spatiotemporal resolution. Moreover, histopathologic analyses of H&E-stained tissue sections indicate that no side effects for mice treated with gold precursors are found during the process of systemic bio-administration for gold precursors. This raises the possibility of utilizing the in vivo biosynthesized GNCs through intravenous administration of biocompatible gold precursors as promising and effective biomarkers for rapid tumor diagnosis and precise surgical intervention.
    RSC Advances 08/2014; 4(71). · 3.71 Impact Factor
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    ABSTRACT: Fluorescent platinum nanoclusters constructed through one-step synthesis from chloroplatinic acid cross swiftly across carcinoma cell membranes for bio-imaging and photothermal treatment.
    RSC Advances 08/2014; · 3.71 Impact Factor
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    ABSTRACT: Ultra-small metallic nanoparticles, or so-called "nanoclusters" (NCs), have attracted considerable interest due to their unique optical properties that are different from both larger nanoparticles and single atoms. To prepare high-quality NCs, the stabilizing agent plays an essential role. In this work, we have revealed and validated that cytidine and its nucleotides (cytidine 5'-monophosphate or cytidine 5'-triphosphate) can act as efficient stabilizers for syntheses of multicolored Au NCs. Interestingly, Au NCs with blue, green and yellow fluorescence emissions are simultaneously obtained using various pH environments or reaction times. The transmission electron microscopy verifies that the size of Au NCs ranges from 1.5 to 3 nm. The X-ray photoelectron spectroscopy confirms that only Au (0) species are present in NCs. Generally, the facile preparation of multicolored Au NCs that are stabilized by cytidine units provides access to promising candidates for multiple biolabeling applications.
    Nanoscale 07/2014; · 6.73 Impact Factor
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    ABSTRACT: Rapid and ultrasensitive detection of pathogenic bacteria and their relevant multidrug resistance is particularly important in clinical diagnosis, disease control, and environmental monitoring. In this contribution, we have explored the possibility to rapidly detect some important disease related bacteria based on nanostructured Au modified ITO electrode through the antibiotic agents such as doxorubicin (DOX). The rapid and real-time electrochemical detection of multidrug resistant bacteria like E. coli and S. aureus could be readily realized through the nanostructured Au based biosensor with high sensitivity. The observations of surface-enhanced Raman spectroscopy and laser confocal fluorescence microscopy also demonstrate the effectiveness of the relevant new strategy for the rapid and ultrasensitive electrochemical detection of some disease related bacteria.
    ACS Applied Materials & Interfaces 06/2014; · 5.90 Impact Factor
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    ABSTRACT: During photodynamic therapy (PDT) of cancers, there are numerous side effects, accompanied by damage to normal cells/tissues caused by the abnormal elevation of reactive oxygen species (ROS). In this paper, we aim to provide an effective method to reduce the relevant side effects of PDT by using cerium oxide nanoparticles. The well-dispersed poly(vinyl pyrrolidone) stabilized cerium oxide nanoparticles were successfully synthesized by using a one-pot method at 60 degrees C in slightly alkaline environment. The morphological and structural characterizations clearly illustrate the excellent lattice structures of cerium oxide, nanoparticles. The MTT assay indicates that these cerium oxide nanoparticles show no intrinsic cytotoxicity even at a concentration up to 300 micro g/mL. More importantly, the results demonstrate that these nanoparticles can selectively protect human normal cells but not the cancer cells from ROS damage after exposure to UV-radiation, suggesting their potential applications for PDT treatment. The rationale behind the selective protection effect can be attributed to the hindrance of the Ce (III)/Ce (IV) redox reaction cycle on the surface of cerium oxide nanoparticles due to the abnormal intracellular pH in cancer cells. Furthermore, these cerium oxide nanoparticles can be used as effective drug carriers for enhancing drug delivery efficiency to target cancer cells like hepatoma HepG2 cells. This raises the possibility of applying cerium oxide nanoparticles for multifunctional therapeutic applications, i.e., combination of efficient PDT and chemotherapy.
    Journal of Biomedical Nanotechnology 02/2014; 10(2):278-86. · 7.58 Impact Factor
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    ABSTRACT: Human life toll by cancer, one of the highest among most dreaded diseases in advanced societies, could be reduced by implementing evidence-based strategies for its prevention, early diagnosis and assessment of the progress and suitability of therapies by fast and non-invasive methods. In this contribution, a novel strategy is reported for highly sensitive recognition and in vivo imaging of cancer cells taking advantage of their spontaneous ability to generate silver nanoclusters (NCs) with high near-infrared fluorescence emission by intracellular reduction of innocuous silver salts. Both ex vivo experiments comparing cancer cell models to normal cells and in vivo imaging of subcutaneous xenografted tumor (cervical carcinoma model) in nude mice established the validity of this strategy for precise and selective imaging of cells and tumors. Furthermore, it was observed that the spontaneous self-generation of Ag NCs by tumors in their inside led to drastic reduction of their sizes and often to complete remission, thus providing important hope for new therapy strategies based on cheap and readily available agents.
    Scientific Reports 01/2014; 4:4384. · 5.08 Impact Factor
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    ABSTRACT: Recently, a growing amount of attention has been focused on the utility of biosensors for biomedical applications. Combined with nanomaterials and nanostructures, nano-scaled biosensors are installed for biomedical applications, such as pathogenic bacteria monitoring, virus recognition, disease biomarker detection, among others. These nano-biosensors offer a number of advantages and in many respects are ideally suited to biomedical applications, which could be made as extremely flexible devices, allowing biomedical analysis with speediness, excellent selectivity and high sensitivity. This minireview discusses the literature published in the latest years on the advances in biomedical applications of nano-scaled biosensors for disease bio-marking and detection, especially in bio-imaging and the diagnosis of pathological cells and viruses, monitoring pathogenic bacteria, thus providing insight into the future prospects of biosensors in relevant clinical applications.
    The Analyst 06/2013; · 4.23 Impact Factor
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    ABSTRACT: The interactions between methylamine or acetic acid with the Ni(n) (n = 1,...,6) in triplet clusters were examined at the B3LYP/6-31G(d, p) and LANL2TZ+ level. The structural stability, interacting strength and the charge transferring properties of the different interacting complexes were studied. The stable distance between the Ni(1) and the nitrogen or oxygen is about 2.00 or 1.95 angstroms for the Ni(n)-CH3NH2 or Ni(n)-CH3COOH, respectively. The results indicate that the interacting energy is -27.385 approximatley -134.269 and -18.379 approximately -55.136 kJ/mol for the Ni(n)-CH3NH2 and Ni(n)-CH3COOH, respectively. The effective electron transfer processes for the Ni(n)-CH3NH2 or Ni(n)-CH3COOH is from the lone pair of the nitrogen to the lone pair anti-bonding orbital of the Ni(1) or from the oxygen lone pair to the sigma antibonding orbitals of Ni(1)-Ni and the sigma anti-bonding orbital of the Ni(1) lone pair, respectively.
    Journal of Nanoscience and Nanotechnology 06/2013; 13(6):4223-7. · 1.15 Impact Factor
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    ABSTRACT: We have studied the multifunctional effects of cysteamine-coated cadmium–tellurium quantum dots (Cys–CdTe QDs) conjugated with gambogic acid (GA) for cancer cell labeling and cancer treatment. Our results indicated that the Cys–CdTe QDs (about 3 nm) could readily bind on the cell plasma membranes and then be internalized into cancer cells for real-time tracing and treatment of human leukemia cancers. The positively charged surface of the self-assembled and conjugated GA with the Cys–CdTe QDs could significantly enhance the drug accumulation into leukemia K562 cells and the drug's cytotoxicity, to inhibit the cancer cell proliferation. The GA–Cys–CdTe nanocomposites improved the drug action to overcome the multidrug resistance of K562/A02 cells and facilitated the GA induced G0/G1 phase cancer cell cycle arrest to promote cell apoptosis. Moreover, the sensitive pH-triggered release behavior of the relevant nanocomposites, loaded with GA, greatly reduced the side effects of the anticancer agents to the normal cells/tissues in the blood circulation and facilitated an efficient drug release and accumulation in the target tumor cells. Thus, the combination of an active compound from Traditional Chinese Medicine (TCM), GA, with Cys–CdTe QDs can afford a new strategy for the potential multimode cancer therapy.
    RSC Advances 04/2013; 3(18):6518-6525. · 3.71 Impact Factor
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    ABSTRACT: As a novel strategy to combat the nosocomial infection caused by multidrug-resistant (MDR) bacteria like Acinetobacter baumannii, the antibacterial effect of a pair of geometrical isomers ferrocene-carborane derivatives (designated as FcSB1 and FcSB2) combining with nanoscaled titania (nano TiO2) against five MDR A. baumannii strains were explored in this contribution. The drug interaction assay and time-kill studies demonstrate the considerable enhancement effect of the TiO2 nanoparticles on the antibacterial activity of FcSB1/or FcSB2 against clinically MDR-resistant A. baumannii. Meanwhile, the relevant antibacterial efficiency assessed by a nonparametric approach (fractional inhibitory concentration [FIC] index model) illustrates that the FIC index values ranged from 0.375 to 0.106 for the combination of FcSB1 and nano TiO2, and 0.250 to 0.083 for that of FcSB2 with nano TiO2, indicating the synergistic antibacterial activity of FcSB1/or FcSB2 with nano TiO2 on target bacteria. This might shed light on the promising bio-application of these new carborane derivatives in combination with nano TiO2 to combat the nosocomial infection caused by multidrug-resistant (MDR) bacteria including A. baumannii.
    Journal of Biomedical Nanotechnology 03/2013; 9(3):393-402. · 7.58 Impact Factor
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    ABSTRACT: Fluorescence imaging in vivo allows non-invasive tumor diagnostic thus permitting a direct monitoring of cancer therapies progresses. It is established herein that fluorescent gold nanoclusters are spontaneously biosynthesized by cancerous cell (i.e., HepG2, human hepatocarcinoma cell line; K562, leukemia cell line) incubated with micromolar chloroauric acid solutions, a biocompatible molecular Au(III) species. Gold nanoparticles form by Au(III) reduction inside cells cytoplasms and ultimately concentrate around their nucleoli, thus affording precise cell imaging. Importantly, this does not occur in non-cancerous cells, as evidenced with human embryo liver cells (L02) used as controls. This dichotomy is exploited for a new strategy for in vivo self-bio-imaging of tumors. Subcutaneous injections of millimolar chloroauric acid solution near xenograft tumors of the nude mouse model of hepatocellular carcinoma or chronic myeloid leukemia led to efficient biosynthesis of fluorescent gold nanoclusters without significant dissemination to the surrounding normal tissues, hence allowing specific fluorescent self-bio-marking of the tumors.
    Scientific Reports 01/2013; 3:1157. · 5.08 Impact Factor
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    ABSTRACT: Daunorubicin (DNR) loaded graphene–gold nanocomposites offer a novel strategy for inducing apoptosis in drug resistant leukemia cells (K562/A02; KA). In vitro and in vivo investigations on xenografted tumors in KA nude mice demonstrate that the combination of monoclonal P-glycoprotein (P-gp) antibodies and DNR anticancer drug loaded on graphene–gold nanocomposites (GGN) is an efficient drug delivery vector, with remarkable targeting and binding properties towards drug resistant KA cell lines, and induces apoptosis of KA cells and inhibits tumor growth in KA nude mice. Cellular treatment with DNR-loaded GGN remarkably reduced drug resistant-related P-gp expression and activated apoptosis-related caspase protein expression in KA cells. Cell apoptosis provoked in vitro by such nanocomposites corresponds to a rapid induction of active caspase 8,3 activities and stimulation of poly-(ADP-ribose) polymerase (PARP) proteolytic cleavage. In vivo studies indicate that DNR-loaded GGN nanocomposites effectively overcome the inhibition of drug resistant leukemia cell-induced tumor growth in KA nude mice. This nanocomposite raises the possibility of modulating apoptosis in cancer cells, and of inhibiting tumor growth, showing that nanocomposites of this kind have promising applications in efficient multifunctional therapy.
    J. Mater. Chem. B. 12/2012; 1(4):493-499.
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    ABSTRACT: Multidrug resistance (MDR) of bacteria is still an unsolved serious problem to threaten the health of human beings. Developing new antibacterial agents, therefore, are urgently needed. Herein, we have explored the possibility to design and synthesize some novel antibacterial agents including ferrocene-substituted carborane derivative (Fc(2)SBCp(1)) and have evaluated the relevant antibacterial action against two clinical common MDR pathogens (i.e., Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa) in vitro and in vivo. The results demonstrate that in vitro antimicrobial activity of Fc(2)SBCp(1) could be gradually transformed into a bactericidal effect from a bacteriostatic effect with the increasing concentration of the active carborane derivative, which can also prevent biofilm formation at concentrations below MIC (i.e., minimal inhibitory concentration). Biocompatibility studies indicate that there exists no/or little toxic effect of Fc(2)SBCp(1) on normal cells/tissues and leads to little hemolysis. In vivo studies illustrate that the new carborane derivative Fc(2)SBCp(1) is highly effective in treating bacteremia caused by S. aureus and P. aeruginosa as well as interstitial pneumonia caused by S. aureus. This raises the possibility for the potential utilization of the new ferrocene-substituted carborane derivatives as promising antibacterial therapeutic agents against MDR bacterial infections in future clinical applications.
    Biomaterials 11/2012; · 8.31 Impact Factor
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    ABSTRACT: Antimicrobial resistance has now become a very serious global public health problem. New drug discovery and development are urgently needed to combat the growing threat of multidrug-resistant (MDR) bacteria. The aim of this study was to explore the potential application of three ferrocene-carborane derivatives as new promising agents to confront the problem of increasing antibiotic resistance. The results of agar diffusion bioassay, minimal inhibitory concentrations (MIC) testing and time-kill assay illustrate their broad-spectrum antimicrobial activities to both American Type Culture Collection (ATCC) control strains and MDR clinical isolates. It is evident that the relevant antimicrobial properties are all in a dose-dependent manner and gradually transform into a bactericidal effect from a bacteriostatic effect with the increasing of the drug concentration. Furthermore, these ferrocene-carborane derivatives have no/little toxic effect on normal cells like HELF cells and lead to little hemolysis at their MICs. This raises the possibility to develop novel antimicrobial drugs using these new ferrocene carborane derivants.
    Science China-Chemistry 11/2012; 55(11). · 1.33 Impact Factor
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    ABSTRACT: High level of oxidative stress is involved in formation of incipient tumor and carcinomatous cells. Here in this contribution we have explored a facile strategy to assess the oxidative stress elicited by hydrogen peroxide (H(2)O(2)) in cells with amperometric current-time technique in vitro. An electrochemical biosensor exhibiting high sensitivity and selectivity to H(2)O(2) is fabricated by integration of graphene with gold nanoparticles and poly(toluidine blue O) films. The efflux of H(2)O(2) from several representative tumor cells and normal cells on exposure to ascorbic acid could be detected by using the graphene-based nanocomposite films. The results indicate that tumor cells release much more H(2)O(2) than do the normal cells. The novel sensor raises the possibility for clinical diagnostic application to evaluate the higher level of intracellular oxidative stress of tumor cells in comparison with normal cells.
    Biosensors & Bioelectronics 10/2012; · 6.45 Impact Factor
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    ABSTRACT: The performance of TiO(2) nanoparticles is extremely attractive in various areas of chemical and biochemical engineering as they can effectively work by combining the photocatalytic property with various superior properties of the related nanostructure. The relevant photoelectrochemical detection has attracted considerable interest and shown potential applications in a wide range of areas. In this study, we have prepared new nanowhiskers of platinum-doped titanium dioxide (TiO(2)-Pt), which could be further used to fabricate a novel nanointerface for the sensitive detection of biomolecules including glutathione (GSH). Our observations demonstrate that the sensitive TiO(2)-Pt nanowhiskers biointerface could be readily fabricated by casting the TiO(2)-Pt nanowhiskers suspension on a glassy carbon electrode (GCE), which could readily combine the photocatalytic and eletrocatalytic properties of TiO(2) nanocomposites to introduce a novel photoelectrocatalytic biosensor for GSH detection in real samples. Compared to other analysis strategies, the TiO(2)-Pt nanowhiskers-modified GCE showed a considerably high sensitivity for the detection of GSH due to the excellent photoelectrocatalytic ability of the porous TiO(2)-Pt nanowhiskers. Scanning electron microscopy (SEM), Raman spectroscopy, and electrochemical impedance spectroscopy have shown that Pt can readily blend with porous TiO(2) nanowhiskers and facilitate the relevant catalysis property of TiO(2), resulting in the enhanced photoelectrocatalytic effect. Thus, through the new strategy of the utilization of the excellent photoelectrocatalytic property of TiO(2)-Pt nanocomposites, it is possible to realize the rapid electrochemical detection of glutathione with high sensitivity, low cost, and good reproducibility.
    Langmuir 08/2012; 28(33):12393-9. · 4.38 Impact Factor
  • Hui Jiang, Xuemei Wang
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    ABSTRACT: Alkaline phosphatase (ALP) catalyzes the hydrolysis and transphosphorylation of a wide variety of phosphoric acid monoesters and plays an important role in clinical diagnosis. In this work, an ALP-responsive anodic electrochemiluminescence (ECL) system based on coreaction of CdSe nanoparticles (NPs) and triethylamine has been designed for facile detection of ALP. The substrate of ALP, i.e., phenyl phosphate salt, shows no effect on the ECL emission whereas its catalytic product of phenol may induce ECL inhibition. For the buffer containing phenyl phosphate, the ECL emission is found to decline in the presence of ALP with different incubation time. The mechanism investigations indicate that the deposition of the electropolymerized phenol products may compete with the electrophoretic-driven adsorption of CdSe NPs on glassy carbon electrode and induce the ECL inhibition, which can be demonstrated by scanning electron microscopy, energy dispersive spectrometry, and anodic stripping voltammetry. Therefore, an inhibition type strategy has been developed to sensitively detect ALP ranging from 0.5 to 6.4 nM (activity ca. 2-25 U/L), with a detection limit of 0.5 nM. The potential interference from the common proteins is negligible. The recovery of ALP in diluted serum samples ranges from 91 to 114%, implicating its potential applications in the complex biological matrixes.
    Analytical Chemistry 07/2012; 84(16):6986-93. · 5.82 Impact Factor
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    ABSTRACT: Drug safety for human body should be carefully studied for its potent clinical application. In this report, the neurotoxicity of anticancer drug daunorubicin (DNR) and the oleic acid-capped Fe3O4 nanoparticles (NPs) for rat brain was firstly explored by using the in vivo microdialysis. The results indicated that the anticancer drug DNR itself had the serious neurotoxicity for the rat brain. And this neurotoxicity was influenced through the concentration changes of amino acids. The concentration level of some excitatory amino acids (such as Glu) and some inhibiting amino acid (such as Gly) were considerably decreased while that of the excitatory amino acid Asp was remarkably increased. For the DNR conjugated with Fe3O4 NPs nanocomposites, the side effect of DNR was visibly cut down, and the time to cause the side neurotoxicity was apparently shortened. Thus, it is evident that compared with DNR alone, the DNR conjugated with Fe3O4 NPs nanocomposites have the better biocompatibility and bio-security for the relevant cancer treatment in vitro and in vivo. This raises the promising possibility of the application of these DNR conjugated with Fe3O4 NPs nanocomposites for the target cancer therapy.
    Journal of Biomedical Nanotechnology 06/2012; 8(3):417-23. · 7.58 Impact Factor

Publication Stats

319 Citations
245.07 Total Impact Points


  • 2008–2014
    • Southeast University (China)
      Nan-ching-hsü, Jiangxi Sheng, China
  • 2012
    • Hong Kong Baptist University
      Chiu-lung, Kowloon City, Hong Kong
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China