Božo Krušlin

Sisters of Charity Hospital Zagreb, Zagreb - Centar, Grad Zagreb, Croatia

Are you Božo Krušlin?

Claim your profile

Publications (62)42.59 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The Zagreb Collection of developing and adult human brains consists of approximately 1,300 brains of fetuses, children and adults that were collected following routine autopsies in the period from 1974 to 2014. The collection comprises brains of different normal developmental stages that may serve for investigation of normal human brain development. Previous studies on this material have led to several important contributions on human cortical development, such as the discovery of the transient fetal subplate zone. The Zagreb Collection, however, also contains approximately 100 brains with different anomalies including chromosomal aberrations such as Down syndrome. We have analyzed all the available material from the Zagreb Collection and identified 44 brains of fetuses and children with Down syndrome, 10 with Patau syndrome, 6 with Edwards syndrome as well as 7 holoprosencephalic, 7 hydrocephalic and 4 microcephalic brains. The largest part of the Collection is available for further research using modern genetic, immunocytochemical and imaging methods, especially magnetic resonance imaging. Furthermore, the histological slides from the Zagreb Collection are currently being digitally scanned and made available as virtual slides to general scientific audience. The Zagreb Collection represents unique and versatile resource for the future study of normal and abnormal human brain development.
    Translational Neuroscience. 12/2014; 5(4).
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Testicular germ cell tumours are the most common malignancies in young males. Molecular biology studies of these tumours are often contradictory. Two histological groups, seminoma and non-seminoma, differ both morphologically and in malignant behaviour. Although a common cytogenetic feature is seen, namely the amplification of the 12p chromosomal region, the development mechanisms of less aggressive seminomas and more aggressive non-seminomas are unknown.
    Anticancer research 08/2014; 34(8):4005-12. · 1.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background Keratocystic odontogenic tumour (KCOT) is a benign, yet aggressive odontogenic tumour. Herein, proteome analysis of KCOT lesions in comparison with control patient-matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented.Methods For the proteomics profiling, two complementary proteomics techniques MALDI-MS/MS and LC-ESI-MS/MS were employed. Potential candidate biomarkers were validated by immunohistochemistry.ResultsMore than 43 proteins were found to be differentially expressed or up-regulated in KCOT lesions in comparison with patient-matched unaffected oral mucosa. These proteins bear important biological functions and are involved in cell proliferation, cytoskeletal re-organization, transcription, cellular motility and apoptosis. In particular, a number of differentially expressed proteins participate in autocrine regulation and signalization within JNK and p38 MAPK signalling pathways.Conclusions Immunohistochemical validation of chosen putative biomarkers revealed axin interaction partner and dorsalization-antagonist (AIDA), known as a protein that blocks activation of JNK signalling pathway, as a differential biomarker for KCOT lesions on an independent cohort of KCOT tissue samples in comparison with most prevalent intra-oseal lesions inflammatory odontogenic cysts.
    Journal of Oral Pathology and Medicine 07/2014; · 2.06 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A B S T R A C T Herein we present 82-year-old man with leiomyosarcoma arising from the spermatic cord with scalp metastasis, five years after primary surgical treatment. Complete surgical excision is required in such cases, as well as precise evaluation of further therapy. Paratesticular leiomyosarcoma is a rare entity, malignant mesenchimal tumor of smooth muscle dif-ferentiation. Although leiomyosarcomas of different localizations have well-known metastatic potential, cutaneous meta-stases are extremely rare with only 16 cases described in the literature. To our knowledge there are no reported cases of the paratesticular leiomyosarcoma metastatic to the skin. This article reviews the literature regarding paratesticular leiomyosarcoma presentation, diagnosis and treatment.
    Collegium antropologicum 06/2014; 38(2):763-766. · 0.61 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Since the discovery of the TCF/LEF family of transcription factors, their functions have been under intensive investigation in the area of cancer biology. The work presented in this paper focused on the changes in TCF-1 and LEF-1 expression levels in a set of astrocytic brain tumors. Protein expression was detected using immunohistochemistry and then evaluated by Ellipse software (ViDiTo, Slovakia). Statistical evaluations were performed with the SPSS statistical package, version 14.0 (SPSS Inc., Chicago, IL, USA). Strong TCF-1 and LEF-1 expression was observed in 51.6% and 71% of glioblastoma samples. Statistical analysis confirmed significant differences in protein expression levels associated to 3 important values, weak expression of TCF-1, weak expression of LEF-1 and strong expression of LEF-1. Analysis of variances performed on the total sample also indicated significant differences in the values of TCF-1 weak (F=2.804; p=0.045), LEF-1 weak (F=4.255; p=0.008) and LEF-1 strong (F=5.498; p=0.002) with regard to malignancy grade. Thus, glioblastomas were characterized by -in relative terms- the lowest values for weak expression of TCF-1 and LEF-1, combined with the highest values of LEF-1 strong expression. The F-ratios for two variables (LEF-1 strong and LEF-1 weak) indicated that differences between astrocytomas (II, III) and glioblastomas were statistically significant (p⟨0.02). Discriminant function analysis further showed that strong LEF-1 expression alone could discriminate between astrocytomas (II, III) and glioblastomas. Elevated TCF-1 and LEF-1 expression is characteristic of malignant gliomas. LEF-1, in particular, may serve as a potential marker for malignant transformation.
    Histology and histopathology 05/2014; · 2.28 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the digestive tract, believed to originate from the interstitial cells of Cajal or their stem cell-like precursors. Recent studies incidentally found the expression in interstitial cells of Cajal of the signal-transducing adaptor molecule-2 (STAM2), which is an endosomal protein acting as a regulator of receptor signaling and trafficking. Here, we investigated the immunohistochemical expression of STAM2 in GIST. To evaluate the level of STAM2 expression, the percentage of cells staining positively for STAM2 and their staining intensity were graded on a scale of 0-3 and then multiplied to give the staining index as: 0=none; 1-3=low; 4-6=moderate and 9=high. In 51 analyzed GIST samples, expression of STAM2 was observed in 45 cases (88.2%). Based on antibody screening, we observed a positive correlation between the expression of GIST marker stem cell growth factor receptor, also known as tyrosine-protein kinase KIT or CD117, and STAM2 expression (r=0.387, p<0.003). To identify possible STAM2 function in GIST, we performed correlation analysis between STAM2 expression and tumor size, primary tumor site, tumor type, mitotic count, Ki-67 proliferative index, risk stratification and development of recurrent/metastatic disease. Among these parameters, only correlation between the percentage of STAM2-positive cells and mitotic count was statistically significant (r=-0.362, p<0.01). Further studies are required to unravel the role of STAM2 in the oncogenic cell phenotype of GIST.
    Anticancer research 05/2014; 34(5):2291-6. · 1.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Adenoid cystic carcinoma (ACC) is a malignant neoplasm that arises within secretory glands. It rarely occurs in the lacrimal gland. Kidney metastases are very rare. We report a case of a 76-year-old female patient treated with right radical nephrectomy because of the tumour mass of the right kidney. Well-circumscribed, firm, grey mass was found on the lower pole of the kidney. Histologically, tumour was considered to be ACC. Retrospective history data showed ACC of the lacrimal gland surgically treated 14 years before with metastasis to the lung operated seven years before. Our diagnosis was metastatic ACC to the kidney. ACC has a remarkable capacity for recurrence. To our knowledge, in the English-speaking area (PubMed), 10 cases of ACC metastatic to the kidney have been described to date, and this is the second reported case of kidney metastasis from primary lacrimal ACC.
    Scottish medical journal 04/2014; · 0.29 Impact Factor
  • Source
    Pathology International 04/2014; 64(4). · 1.72 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer-related deaths among female population worldwide. Metastases are the common cause of morbidity and mortality in breast cancer and can remain latent for several years after surgical removal of the primary tumour. Thus, the identification and functional characterisation of molecular factors that promote oncogenic signalling in mammary tumour development and progression could provide new entry points for designing targeted therapeutic strategies for metastatic breast cancer. In the present study, we investigated the expression of proteins involved in cell signalling (growth hormone receptor (GHR) and NEDD9) and cell-cell adhesion (plakoglobin) in epithelial and stromal compartments of primary ductal invasive breast carcinomas and their axillary lymph node metastases versus non-metastatic tumours. Obtained data revealed remarkable increase in the expression levels of GHR and NEDD9 proteins in both epithelial and stromal components of axillary lymph node metastases in comparison with those of non-metastatic tumours, suggesting that the expression of these two proteins may provide biomarkers for tumour aggressiveness.
    Tumor Biology 03/2014; · 2.52 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: ABSTRACT Abstract Objective.The aim of this study was to correlate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) with pathological renal artery changes in patients with renal cell carcinoma (RCC). A further aim was to correlate intratumoral microvessel density (MVD) with VEGF and HIF-1α expression and prognostic factors for RCC, including tumour necrosis. Material and methods. Formalin-fixed and paraffin-embedded tissue blocks from 150 patients with RCC and 50 patients with non-tumorous kidney diseases were analysed. The control group consisted of specimens from both renal arteries obtained from 25 decedents at routine autopsy (50 cases in total). Immunohistochemistry was performed using primary antibodies to VEGF, HIF-1α and CD31. Results.Pathological renal artery changes were more common in patients with RCC and non-tumorous kidney diseases than in the control group. MVD was higher in the RCCs of patients with pathological renal artery changes. Tumours with higher HIF-1α expression had higher MVD; however, VEGF expression was not associated with MVD. A significant association was also found between MVD and the extent of tumour necrosis, in that less necrotic tumours had higher MVD. No association between renal artery changes and VEGF and HIF-1α expression was established. Conclusion. Considering the results of this study, the evaluation of renal artery changes in forthcoming research on RCC would be helpful for several reasons: to estimate their incidence in a larger number of patients, to clarify their connection with RCC and to reveal their relationship with MVD in RCC.
    Scandinavian Journal of Urology and Nephrology 02/2014; 48(1):34-40. · 1.01 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Objective.The aim of this study was to correlate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) with pathological renal artery changes in patients with renal cell carcinoma (RCC). A further aim was to correlate intratumoral microvessel density (MVD) with VEGF and HIF-1α expression and prognostic factors for RCC, including tumour necrosis. Material and methods. Formalin-fixed and paraffin-embedded tissue blocks from 150 patients with RCC and 50 patients with non-tumorous kidney diseases were analysed. The control group consisted of specimens from both renal arteries obtained from 25 decedents at routine autopsy (50 cases in total). Immunohistochemistry was performed using primary antibodies to VEGF, HIF-1α and CD31. Results.Pathological renal artery changes were more common in patients with RCC and non-tumorous kidney diseases than in the control group. MVD was higher in the RCCs of patients with pathological renal artery changes. Tumours with higher HIF-1α expression had higher MVD; however, VEGF expression was not associated with MVD. A significant association was also found between MVD and the extent of tumour necrosis, in that less necrotic tumours had higher MVD. No association between renal artery changes and VEGF and HIF-1α expression was established. Conclusion. Considering the results of this study, the evaluation of renal artery changes in forthcoming research on RCC would be helpful for several reasons: to estimate their incidence in a larger number of patients, to clarify their connection with RCC and to reveal their relationship with MVD in RCC.
    Scandinavian journal of urology. 11/2013;
  • Source
    Dataset: UrolOncol
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND: The epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein that was originally identified as a marker for carcinoma, attributable to its high expression on rapidly proliferating tumors of epithelial origin. The role of EpCAM is not limited to cell adhesion but includes diverse processes such as signaling, cell migration, proliferation, and differentiation. OBJECTIVE: Several studies investigated EpCAM expression in prostate carcinoma but none of them confirmed its prognostic role. The aim of our study was to investigate EpCAM expression and its relationship with established prognostic features in prostate carcinoma. MATERIALS AND METHODS: The study included a cohort of 102 patients treated with radical prostatectomy for clinically localized prostate carcinoma. Immunohistochemistry was performed to evaluate the EpCAM expression in prostate cancer and non-neoplastic prostate tissue. The percentage of positively stained carcinoma and benign glands was examined in the whole mount of the chosen slide. RESULTS: The extent of EpCAM expression was significantly higher in malignant than in benign prostatic tissue (P < 0.001). EpCAM expression in prostate cancer was associated with established features indicative of worse prognosis, such as preoperative (P = 0.009) and postoperative (P = 0.004) Gleason score and follow-up time (P < 0.001). Patients with higher preoperative and postoperative Gleason score and short follow-up time had tumors with a significantly higher expression of EpCAM. Negative correlation of follow-up time and EpCAM expression indicated that tumors in patients with biochemical recurrence (BCR) harbored higher EpCAM expression. Moreover, expression of EpCAM was significantly higher in patients with BCR compared with patients without BCR (P < 0.001). Tumors in T3 stage of the disease showed significantly higher EpCAM expression compared with T2 tumors (P = 0.002). Univariate (P < 0.001) and multivariate (P < 0.001) analyses showed that EpCAM expression was a significant predictor of shorter biochemical recurrence free-survival. CONCLUSION: Our results confirmed high level of EpCAM expression in prostate cancer and support its potential role in prostatic cancer progression. In addition, EpCAM could serve as an additional prognostic marker for the recognition of patients with an increased risk of disease recurrence that need introduction of secondary therapy.
    Urologic Oncology 04/2013; · 3.65 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mijelolipom je rijedak, dobroćudan, nefunkcionalni tumor, najčešće smješten u kori nadbubrežne žlijezde. Sastoji se od zrelog masnog tkiva s komponentama hematopoetskog tkiva u različitom omjeru. Postoje stanovite nedoumice vezane uz dijagnosticiranje i liječenje mijelolipoma pa je zbog toga važno sagledati sve aspekte te lezije i okolnosti u kojima se ona pojavljuje. Ovdje prikazujemo seriju od 15 bolesnika s mijelolipomom dijagnosticiranih na Zavodu za patologiju “Ljudevit Jurak” KBC-a “Sestre milosrdnice”. Od 15 bolesnika desetorica su bili muškarci (jedan s bilateralnim tumorom) u dobi od 4 do 73 godine te pet žena u dobi od 51 do 54 godine. Makroskopski, tvorbe su bile ovalne, inkapsulirane, žućkaste, mekane mase smještene u nadbubrežnim žlijezdama. Promjer tumora kretao se od 0,5 do 13,9 cm. Mikroskopski su bili građeni od umnoženih zrelih masnih stanica u kombinaciji s mijeloidnim tkivom koje se pretežno sastojalo od megakariocita, eritroidnih stanica i limfocita. Nije bilo znakova atipije. Bolesnici su nakon kirurškog zahvata dobro i bez recidiva. Ključne riječi: nadbubrežna žlijezda, mijelolipom
    Acta medica Croatica. 01/2013; 67:255-258.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Large cell neuroendocrine carcinoma (LCNEC) is an aggressive neoplasm with a low frequency of occurrence in the digestive tract. We present a series of eight patients diagnosed with LCNEC of the colon and rectum. Grossly, tumors were presented as endophytic/ulcerative, annular and polypoid masses, with a gray-white color and necrosis in most cases. Histologically, they were high-grade tumors composed of large cells of organoid, nesting, trabecular, rosette-like and palisading patterns, with a high mitotic rate. Tumors were immunoreactive for neuroendocrine markers, including chromogranin A (2/8), synaptophysin (7/8), and neuron-specific enolase (8/8). Moreover, we analyzed the expression of growth hormone (hGH) and growth hormone receptor (GHR) in colorectal LCNECs and six tumors were immunoreactive for hGH, while five tumors were immunoreactive for GHR. To our knowledge hGH and GHR expression has not been previously analyzed in colorectal LCNEC. Their overexpression suggests a role of hGH and GHR in the development of colorectal LCNEC.
    Anticancer research 08/2012; 32(8):3377-81. · 1.71 Impact Factor
  • Source
    Pathology 06/2012; 44(5):482-5. · 2.66 Impact Factor
  • Source
    Iva Brčić, Borislav Spajić, Božo Krušlin
    [Show abstract] [Hide abstract]
    ABSTRACT: Cases of adult renal cell carcinoma with rhabdoid features have been reported in the literature, usually in association with conventional clear cell and papillary tumors. Till date, only one report described chromophobe renal cell carcinoma with rhabdoid differentiation. We report a case of a 47-year-old male patient that underwent a routine medical check-up. Computer tomography scan revealed a large heterogeneous mass in the left kidney, multiple nodular changes in the lungs and multiple enlarged lymph nodes. On microscopic examination of the left nephrectomy specimen, 65 % of the kidney tumor was comprised of rhabdoid foci and the rest of the tumor showed eosinophilic areas consistent with chromophobe renal cell carcinoma. Final diagnosis was chromophobe renal cell carcinoma with rhabdoid differentiation. Behavior of these tumors in adults is more aggressive, demands more aggressive treatment and has poor prognosis. Therefore, rhabdoid differentiation should be recognized in this type of carcinoma and mentioned in the pathohistology report.
    Wiener klinische Wochenschrift 06/2012; 124(11-12):419-21. · 0.81 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Glioblastoma is the most frequent, the most malignant and the best vascularised primary brain tumor. Substantial evidence suggests that IGFBP-2 (insulin-like growth factor binding protein) may play a significant role in the development and progression of various types of cancer, including the anaplastic progression of astroglial tumors. The correlation of IGFBP-2 and VEGF over-expression in diffuse gliomas and strong IGFBP-2 immunostaining and co-expression with VEGF in the cells of pseudopalisading necrosis suggests the induction of IGFBP-2 expression by hypoxia and/or possible involvement of its protein product in angiogenesis. The aim of this study was to analyze the expression of IGFBP-2 and the relationship between the IGFBP-2 expression, extent of bizarre angiogenesis and the presence of pseudopalisades. The prognostic impact of these variables was estimated, as well. Our results revealed that most glioblastoma (75%) express IGFBP2 and that IGFBP2 expression was associated with the presence of pseudopalisading necrosis as well as extensive bizarre angiogenesis. IGFBP-2 immunoreactivity in more than 5% of tumor cells and the presence of pseudopalisading necrosis were prognostically significant in univariate analysis (p=0.034) while in multivariate analysis only a patient’s age and the presence of pseudopalisades remained statistically significant (p=0.004). The results of our study showed the association of IGFBP-2 expression in glioblastoma with pseudopalisades and bizarre angiogenesis thus confirming on morphological grounds presumed induction of this gene under hypoxic conditions and its possible role in angiogenesis. KeywordsIGFBP-2–Angiogenesis–Pseudopalisades–Glioblastoma–Glioma
    Translational Neuroscience. 04/2012; 2(3):219-224.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Introducción El schwannoma es un tumor benigno, encapsulado, de la vaina de mielina, originándose en las células de Schwann. El schwannoma primario de la glándula suprarrenal es muy infrecuente. Caso clínico Se presenta un caso de una paciente de 55 años, diagnosticada de cáncer de mama y tratada con mastectomía, linfadenectomía y terapia hormonal. Tres meses tras la mastectomía se detectó ecográficamente un tumor, sin actividad hormonal, en la glándula suprarrenal izquierda. Se sospechó una metástasis y se realizó una adrenalectomía. La histología e inmunohistoquímica del tumor fueron consistentes con schwannoma. Conclusión Debido a la poca frecuencia del schwannoma suprarrenal pueden ocurrir problemas diagnósticos y deben diferenciarse de otras lesiones de células de la glándula suprarrenal o de tumores metastásicos.
    Corrosion Science - CORROS SCI. 04/2012;
  • Pathology International 12/2011; 61(12):778-80. · 1.72 Impact Factor

Publication Stats

40 Citations
42.59 Total Impact Points

Institutions

  • 2014
    • Sisters of Charity Hospital Zagreb
      Zagreb - Centar, Grad Zagreb, Croatia
  • 2011–2014
    • University of Zagreb
      • • Department of Biochemical Engineering
      • • Department of Pathology
      Zagrabia, Grad Zagreb, Croatia
    • General Hospital, Nova Gradiska
      Újgradiska, Brodsko-Posavska, Croatia
  • 1999–2014
    • University Clinical Hospital Center "Sestre Milosrdnice"
      Zagrabia, Grad Zagreb, Croatia
  • 2012
    • University Hospital Centre Zagreb
      • Department of Pathology and Cytology
      Zagreb, Grad Zagreb, Croatia