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ABSTRACT: To study the characteristics of the metabolic syndrome (MS) in patients with polycystic ovary syndrome (PCOS).
Oral glucose tolerance test (OGTT) was performed in 336 patients with PCOS, and the serum levels triglycerides (TG), total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and testosterone were measured.
The total incidence of MS was 18.8% in these 336 patients with PCOS. The incidence of MS increased with percent body fat (%BF) and lipid accumulation product (LAP) in patients with PCOS. The patients at child-bearing age appeared to have a higher incidence of MS than those in puberty. The muscle distribution coefficient, age, body mass index, and the metabolic parameters were all higher in patients with MS than in those without MS. The bilateral lower limb muscle strength were lower in patients with MS than in those without, but the level of testosterone showed no significant difference between them.
The risk of MS increases with BF%, age and LAP in patients with PCOS.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 09/2012; 32(9):1325-7.
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ABSTRACT: To investigate association between glutathione S-transferases (GSTs) and cervical cancer.
Published literature from PubMed, EMBASE, and other databases were retrieved. All studies evaluating the association between GSTM1/GSTT1 polymorphisms and cervical were included. Pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using fixed- or random-effects model.
A total of 15 case-control studies were included in the meta-analysis of GSTM1 genotypes (1,825 cases and 2,104 controls). The overall result showed that the association between GSTM1 null genotype and risk for cervical cancer was statistically significant (OR=1.53, 95%CI=1.18-2.00). Great heterogeneity was found between studies. Subgroup analysises were performed based on smoking and ethnicity. Our results showed that smokers with null GSTM1 genotype had higher risk of cervical cancer (OR=1.56, 95%CI=1.01-2.41). For the ethnicity stratification, significant increased risk of null GSTM1 genotype was found in Chinese and Indian population, but no increased risk in other population.
This meta-analysis provides strong evidence that the GSTM1 null genotype is associated with the development of cervical cancer, and especially in Chinese and Indian population, and smoking shows a modification on the association between GSTM1 null genotype and cervical cancer.
Asian Pacific Journal of Tropical Medicine 06/2012; 5(6):480-4. · 0.37 Impact Factor
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Ting-ting Li, Liang-zhi Xu,
Yong-heng Chen,
Hong-mei Deng,
Chun-ying Liang,
Ying Liu,
Xiao-fang Liu,
Jing Zhang,
De-ying Kang,
Dong-sheng Qiu,
Dai-wen Han,
Rong Hu
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ABSTRACT: To investigate the relationship between 8 endocrine-disrupting chemicals in the serum and insulin resistance in patients with polycystic ovary syndrome (PCOS).
This study was conducted among 60 patients with PCOS, including 23 with insulin resistance (PCOS-IR) and 37 without insulin resistance (PCOS-NIR), and 29 non-PCOS women seeking medical attention for infertility or menstrual disorder (control group). The serum levels of 6 phthalic acid esters (PEAs), bisphenol A (BPA) and octylphenol (OP) were measured in all the subjects.
The levels of PAEs, BPA and OP showed no significant differences between PCOS patients and the control group (P>0.05). The serum level of OP was significantly lower in patients PCOS-IR than in those with PCOS-NIR (47.89 ng/ml vs 60.24 ng/ml, P<0.05).
PEAs and BPA do not produce obvious effect on the pathogenesis of PCOS or contribute to insulin resistance, but OP may play a role in insulin resistance in PCOS patients.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 10/2011; 31(10):1753-6.
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ABSTRACT: Conjugated equine estrogen (CEE) treatment, a hormone replacement therapy, is restricted for use in perimenopausal and postmenopausal women because of security issues. Consequently, traditional Chinese herbal medicine has become an alternative choice for the patients with contraindications to hormone replacement therapy.
To evaluate the efficacy and safety of Kuntai Capsule and CEE in treating cognitive function disorder and mental symptoms of early postmenopausal women.
A total of 57 cases of early postmenopausal women from Outpatient Department of West China Women and Children's Hospital were included. The subjects were randomly divided into two groups: Kuntai group with 28 cases and CEE group with 29 cases. The patients in Kuntai group received 6 g Kuntai capsules three times a day. The patients in CEE group received CEE 0.3 mg and 0.6 mg alternately once a day (average dose of 0.45 mg/d). The patients with intact uterus in CEE group were treated with 2 mg medroxyprogesterone acetate daily.
In one-year treatment course, the recognition function and mental symptoms of each patient were investigated by questionnaires of Mini-Mental State Examination (MMSE), Kupperman, and quality of life (QOL) every three months. Both intention-to-treat (ITT) and per-protocol set (PPS) analyses were done.
The MMSE, Kupperman index and QOL scores at each time point were improved as compared with those before treatment (P<0.05), however there were no statistical differences between the two groups (P>0.05). The MMSE scores showed a tendency to escalate while mental symptoms investigated by Kupperman index and QOL scale showed a downtrend. No severe adverse effects occurred in the study phase and no statistical difference in incidence of side effects between the two groups was found except for vaginal bleeding. The incidence rates of vaginal bleeding in CEE and Kuntai groups were 39.3% and 11.1% respectively (P=0.029).
Both Kuntai Capsule and CEE may contribute to maintain the cognitive function and ameliorate mental symptoms of early postmenopausal women.
Journal of Chinese Integrative Medicine 04/2010; 8(4):321-7.
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ABSTRACT: To evaluate the effect of Premarin and Kuntai capsule (a traditional Chinese patent medicine) on the quality of life (QOL) and their cost-utility in early postmenopausal women.
Fifty-seven women with menopausal syndrome in the early postmenopausal stage were randomly allocated into Premarin group (0.3 mg/day and 0.6 mg/day alternately, n=29) and Kuntai group (4 g/day, n=28). The therapies lasted for one year and the patients were followed up every 3 months. The QOL of the patients was evaluated and the utility scores were obtained from rating scale to conduct a cost-utility analysis (CUA).
At each follow-up examination, no significant difference was found in the QOL between the two groups (P>0.05). The QOL obviously increased after the 1-year-long therapy in both the groups, and Kuntai required longer treatment time than Premarin to take effect. The cost-utility ratio of Premarin and Kuntai were 13581.45 yuan/QALY (quality adjusted life year) and 25105.12 yuan/QALY, respectively. Both incremental cost analysis and sensitivity analysis showed that Kuntai was more costly than Premarin. The result of per-protocol analysis was consistent with that of intention-to-treat analysis.
At early stage of menopause, the QOL of women with menopausal syndrome can be significantly improved by low-dose Premarin and Kuntai capsule, but the latter is more costly.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 11/2009; 29(11):2181-6.
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ABSTRACT: To investigate the tyrosine autophosphorylation of insulin receptors in patients with polycystic ovary syndrome (PCOS).
Forty patients with PCOS and 20 age- and body mass index matched healthy women were included in the study. The patients with PCOS were classified as HI-PCOS (n=20) or non-HI-PCOS (n=20) based on the fasting insulin level (>or< or =15 mIU/L). 1) Serum gonadotropins and ovarian steroids were determined. Oral glucose tolerance tests (OGTT) and insulin releasing tests were performed to calculate the insulin resistance index (HOMA-IR). 2) Blood samples were obtained at five time-points during the OGTT (Fasting and 30 min, 60 min, 120 min, and 180 min after taking 75 g glucose orally). The erythrocytes were isolated and the autophosphorylation insulin receptors (APIR) and total insulin receptors (TIR) were measured by enzymatic immunoassay. 3) The in vivo autophosphorylation of insulin receptors was indicated by the APIR/TIR ratio.
The HI-PCOS patients had lower APIR/TIR ratio than the non-HI-PCOS patients and healthy controls at the 60th minute after OGTT (P<0.05). No differences in other time-points were significant.
The autophosphorylation of insulin receptors in HI-PCOS patients decrease, which might be a mechanism for insulin resistance in patients with PCOS.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 09/2009; 40(5):877-80, 892.
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ABSTRACT: To evaluate the effect of weight on bone mineral density in ovariectomic rats.
Forty 6-month-old Wistar female rats were randomly divided into the sham surgical group and the ovariectomic group. The weights of the rats and the bone mineral densities and bone mineral contents of the femurs of the rats were measured eight weeks after the operations.
The body weights of the ovariectomic rats increased significantly. The vaginal cytology of the ovariectomic rats showed no estrous cycle. The areas of the uterus transverse section of the ovariectomic rats deceased significantly. The depth of endometrium and its epithelia, and the area percentage of endometric gland of the ovariectomic rats were smaller than those of the sham surgical controls. There were no statistically differences in the BMD and BMC between the ovariectomic rats and the sham surgical controls. However, the BMC/weight ratio of the ovariectomic rats decreased significantly. The linear stepwise regression analysis indicated a positive correlation between BMC and body weight and a negative correlation between BMC and the weight differences pre- and post operations. The BMC/weight ratio also had a negative correlation with the weight differences.
The osteoporotic animal model after ovariectomy has been successfully established. The certain increased body weight after ovariectomy may slow the lose of bone mass. But the over increasing in body mass lead to BMC/weight decling, caused by osteoporosis.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 02/2009; 40(1):145-8.
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ABSTRACT: To test the reliability and validity of International Physical Activity Questionnaires (long self-administrated format) (IPAQ), Chinese version on women of 12 - 44 years old, in Chengdu city.
Clustered sampling was used, according to the age distribution of investigated population. One community, one university and two middle schools (including one high school and one junior high school) in Chengdu were selected. The reliability study was conducted in a 28-day period. Participants were contacted three times within 28 days (on the 1(st), the 8(th) and the 28(th) day respectively). In the validity study, participants completed the same questionnaire and Bouchard physical activity diary (PA diary) each day for seven days between visit 1 and visit 2 before comparing the IPAQ's result with Bouchard physical activity diary's for validity study. Pearson or Spearman' correlation coefficients were calculated for validity and interclass correlation coefficients for reliability according to date distribution.
(1) One hundred and eighty six participants were recruited for the reliability study. All of them completed the first questionnaire with 143 (76.88%) and 172 (92.50%) finished the second and third questionnaires. One hundred and fifty eight participants were recruited in the validity study. Qualified questionnaire accounted for 90.59% (143). (2) Results from the test-retest reliability of the Chinese version of long self-administrated format (IPAQ) showed: the ICC after one week ranged from 0.7373 to 0.972 (mean: 0.870) which were higher than those interval's reliability [ICC = 0.472 - 0.948 for three weeks interval (mean: 0.721), and 0.473 - 0.925 for four weeks interval (mean: 0.696)]. The ICCs of three weeks interval and four weeks interval were not significantly different. ICCs for vigorous physical activity and leisure time physical activity were the best. (3) The validity for different intensity, total physical activity and sitting time showed the correlation coefficients between IPAQ and PA diary ranged from 0.445 to 0.696, with correlation for vigorous physical activity the best (r = 0.696).
IPAQ-C seemed a reliable and validated measure of physical activity for 12 - 44 year-old women in Chengdu city.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 12/2008; 29(11):1078-82.
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ABSTRACT: To identify the effect of IGF-1 and leptin on the hyperinsulinemia (HI) of patients with polycystic ovarian syndrome (PCOS).
The case-control compared method was applied to the study. Ninety-two PCOS and 92 control subjects were involved in the study. The group of PCOS was further divided into two subgroups of 46 PCOS-HI and 46 PCOS-NHI. The leptin, IGF-1 and other hormones such as serum testosterone, DHT, DHEA, DHEA-S were measured by RIA method.
Leptin and IGF-1 were higher in PCOS group (16.8 +/- 9.8 ng/mL, 214.8 +/- 131.6 ng/mL) than those in control group (11.6 +/- 6.8 ng/ mL, 118.0 +/- 82.9 ng/mL respectively, P < 0.05). Serum leptin level was higher in hyperinsulinemia group (9.2 +/- 10.2 ng/mL) than that in normal insulin group (12.5 +/- 7.6 ng/mL, P < 0.05). IGF-1 (208.7 +/- 109.7 ng/mL vs. 151.7 +/- 120.0 ng/mL, P = 0.66) and fasting glucose (5.1 +/- 0.5 mmol/L vs. 4.9 +/- 0.4 mmol/L, P = 0.15) had no significant difference between two groups. Multiple logistic regression showed that after the body mass index (BMI) adjusted, the testosterone, LH/FSH, DHT, DHEA, and DHEA-S, leptin and IGF-1 showed to be the independent risk factor for PCOS, but not to be for hyperinsulinemia in patients with PCOS.
The leptin and IGF-1 may contribute to the pathogenesis of PCOS, but the relationship between leptin, IGF-1 and hyperinsulinemia needs more researches to confirm.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 12/2006; 37(6):882-5.
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ABSTRACT: This study evaluated the effects of different cryoprotectants and cryopreservation protocols on the development of in vivo fertilized 2-4 cell mouse embryos. Mouse embryos were cryopreserved by using propylene glycerol (PG), ethylene glycerol (EG), dimethyl sulfoxide (DMSO) or glycerol (G) as cryoprotectant with slow-freezing or Vit-Master vitrification protocol. After thawing, the survival rate, blastocyst formation rate and blastocyst hatching rate of the embryos were compared. When mouse embryos were cryopreserved by the slow-freezing, survival rate, blastocyst formation rate and blastocyst hatching rate of the embryos with PG were significantly higher than those of DMSO and G (P < 0.05, respectively), but there is no significantly difference among those of DMSO, G and EG(p > 0.05), and between PG and EG. When mouse embryos were cryopreserved by Vit-Master vitrification, survival rate, blastocyst formation rate and blastocyst hatching rate of the embryos with EG were significantly higher than those of PG, DMSO and G (P < 0.05). There were no significant differences among those of PG, DMSO and G (p > 0.05). In conclusion, PG was the optimal cryoprotectant for the cryopreservation of 2-4 cell mouse embryos by slow-freezing protocol. EG was the optimal cryoprotectant for the cryopresevation of 2-4 cell mouse embryos by Vit-Master vitrification protocol, which may be commonly used in clinical and laboratory practice.
Cryo letters 32(3):240-7. · 1.25 Impact Factor
