Luís Belo

University of Porto, Oporto, Porto, Portugal

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Publications (121)246.32 Total impact

  • Blood Reviews 08/2015; DOI:10.1016/j.blre.2015.07.006 · 5.45 Impact Factor
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    ABSTRACT: To determine the effects of a school-based exercise intervention programme on cardiovascular risk factors, including body fat (BF), metabolic profile and physical activity (PA) in children with and without individualised dietary counselling approach (IDC and WIDC). Forty-six overweight children from 6-16 years old (25 girls, 54.3%; age = 10.3 ± 2.8) of six schools took part in an 8-month interdisciplinary, school-based intervention programme. All children were engaged in PA classes, but only one group was exposed to individualised counselling. Blood pressure (BP), lipids and lipoproteins, accelerometer-based PA, percentage of body fat (%BF) and trunk fat (%TF) measures were taken before and after intervention. General Linear Model (Repeated Measures ANOVA) adjusted for age, maturation and height change was used to analyse the longitudinal effect of individualised counselling between two evaluations in each group. Favourable changes were observed for %BF, %TF, systolic BP and total cholesterol in the IDC group. Subjects WIDC only increased light and moderate-vigorous PA. In IDC, significant effects for time * group interactions were found for systolic BP, total cholesterol and LDL-cholesterol, indicating that counselling might add favourable changes in these markers, beyond those explained by PA and growth. School-based interventions can contribute to counteracting obesity in youth, particularly when individualised dietary counselling is provided. Therefore, the link between schools and professional counselling should be strengthened to ensure consolidated changes towards healthy behaviours.
    Annals of Human Biology 07/2015; DOI:10.3109/03014460.2015.1059889 · 1.15 Impact Factor
  • Appetite 06/2015; 89:323. DOI:10.1016/j.appet.2014.12.074 · 2.69 Impact Factor
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    Medicine and Science Sports Exercise Vol.47, Nº5 Supplement, San Diego; 05/2015
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    ABSTRACT: PURPOSE: To evaluate interventions for improving fundamental motor skills (FMS) and physical activity (PA) levels in preschoolers. Mastery in FMS is associated with greater participation in PA in school aged children. Early childhood has been identified as the critical time for engaging in FMS development, however, there is limited research investigating this relationship in preschoolers aged 3-5 years. METHOD: We conducted a systematic search of electronic databases up until 31st October 2014. Randomised control trials (RCTs) using PA interventions with FMS as outcome measures in preschoolers were eligible for inclusion. Studies including children with disabilities or developmental delays were excluded. Between group mean differences, relative effect sizes (ES) and 95% confidence intervals were calculated for each outcome. RESULTS: Search terms yielded 1411 articles of which 8 RCTs and 2747 participants met the inclusion criteria. Studies were dichotomised into two groups (i) Teacher-Led (TL) Interventions (n=7) and (ii) Child-Centred (CC) Interventions (n=1). Mean age of children studied was 4.0±0.8yrs, with an equal gender distribution. Mean body mass index (BMI) was 16.52±0.23. On average interventions ran for 24±10wks, 3±1 times per week for 27±6mins. Five of the 7 TL studies reported significant improvements in FMS (ES Range 0.21-0.85;p≤0.01). The CC intervention also reported significant improvements in FMS, p≤0.001. Five TL interventions reported changes in PA levels, with one reporting significant improvements (ES 0.47;p≤0.01). Four studies reported body composition, with one reporting a significant decrease in BMI (p=0.02) and waist circumference (p=0.002). CONCLUSION: There is emerging evidence that PA interventions can improve FMS in preschoolers; however, there is a dearth of evidence on CC interventions, where an intervention is delivered directly to children by a trained professional. Targeting FMS development in this age group may promote higher PA levels throughout childhood, however more study is needed. Long term follow up is required to determine if improving FMS at preschool age increases participation in sports and PA. In addition, will it lead to lifestyle patterns throughout adolescence and adulthood, that will reduce the risk of metabolic and cardiovascular diseases. 1423 Board #216 May 28, 8:00 AM-9:30 AM Effects Of 6-month Soccer And Traditional Physical Activity Programs On Body Composition, Cardiometabolic, Inflammatory And Oxidative Markers In Obese Boys
    62nd ACSM Annual Meeting, San Diego; 05/2015
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    ABSTRACT: Anemia is a common complication of chronic kidney disease (CKD) that develops early and its severity increases as renal function declines. It is mainly due to a reduced production of erythropoietin (EPO) by the kidneys; however, there are evidences that iron metabolism disturbances increase as CKD progresses. Our aim was to study the mechanisms underlying the development of anemia of CKD, as well as renal damage, in the remnant kidney rat model of CKD induced by 5/6 nephrectomy. This model of CKD presented a sustained degree of renal dysfunction, with mild and advanced glomerular and tubulointerstitial lesions. Anemia developed 3 weeks after nephrectomy and persisted throughout the protocol. The remnant kidney was still able to produce EPO and the liver showed an increased EPO gene expression. In spite of the increased EPO blood levels, anemia persisted and was linked to low serum iron and transferrin levels, while serum interleukin (IL)-6 and high sensitivity C-reactive protein (hs-CRP) levels showed the absence of systemic inflammation. The increased expression of duodenal ferroportin favours iron absorption; however, serum iron is reduced which might be due to iron leakage through advanced kidney lesions, as showed by tubular iron accumulation. Our data suggest that the persistence of anemia may result from disturbances in iron metabolism and by an altered activity/function of EPO as a result of kidney cell damage and a local inflammatory milieu, as showed by the increased gene expression of different inflammatory proteins in the remnant kidney. In addition, this anemia and the associated kidney hypoxia favour the development of fibrosis, angiogenesis and inflammation that may underlie a resistance to EPO stimuli and reduced iron availability. These findings might contribute to open new windows to identify putative therapeutic targets for this condition, as well as for recombinant human EPO (rHuEPO) resistance, which occurs in a considerable percentage of CKD patients.
    PLoS ONE 04/2015; 10(4):e0124048. DOI:10.1371/journal.pone.0124048 · 3.23 Impact Factor
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    PLoS ONE 03/2015; · 3.23 Impact Factor
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    ABSTRACT: Toxic effects of ultraviolet (UV) radiation on skin include protein and lipid oxidation, and DNA damage. The latter is known to play a major role in photocarcinogenesis and photoaging. Many plant extracts and natural compounds are emerging as photoprotective agents. Castanea sativa leaf extract is able to scavenge several reactive species that have been associated to UV-induced oxidative stress. The aim of this work was to analyze the protective effect of C. sativa extract (ECS) at different concentrations (0.001, 0.01, 0.05 and 0.1μg/mL) against the UV mediated-DNA damage in a human keratinocyte cell line (HaCaT). For this purpose, the cytokinesis-block micronucleus assay was used. Elucidation of the protective mechanism was undertaken regarding UV absorption, influence on (1)O2 mediated effects or NRF2 activation. ECS presented a concentration-dependent protective effect against UV-mediated DNA damage in HaCaT cells. The maximum protection afforded (66.4%) was achieved with the concentration of 0.1μg/mL. This effect was found to be related to a direct antioxidant effect (involving (1)O2) rather than activation of the endogenous antioxidant response coordinated by NRF2. Electrochemical studies showed that the good antioxidant capacity of the ECS can be ascribed to the presence of a pool of different phenolic antioxidants. No genotoxic or phototoxic effects were observed after incubation of HaCaT cells with ECS (up to 0.1μg/mL). Taken together these results reinforce the putative application of this plant extract in the prevention/minimization of UV deleterious effects on skin. Copyright © 2015 Elsevier B.V. All rights reserved.
    Journal of Photochemistry and Photobiology B Biology 01/2015; 144C. DOI:10.1016/j.jphotobiol.2015.01.010 · 2.80 Impact Factor
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    ABSTRACT: Circulating cell-free DNA (cfDNA) may result from cell damage and DNA fragments release, and, therefore, might be increased in inflammatory conditions. Preeclampsia (PE), an important cause of maternal and fetal mortality, is an inflammatory and oxidative condition. Our aim was to quantify cfDNA in Portuguese preeclamptic pregnancies, in order to search for a relationship with inflammation, endothelial dysfunction and severity of the disease. Maternal blood was collected from 31 normal pregnant women and from 33 PE pregnant women in the 3rd trimester. Circulating cfDNA was measured in serum samples using a rapid direct fluorescent assay (Goldshtein et al. Ann Clin Biochem 2009;46:488-94), which does not require prior processing of samples, there is no need for DNA extraction and amplification, is accurate, sensitive, and reproducible. TNF-alpha, a pro-inflammatory cytokine and endothelial markers, tissue plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1) antigen and soluble vascular cell adhesion molecule (sVCAM) levels, were evaluated by enzyme-immunoassay. Cf-DNA, TNF-α, sVCAM, tPA and PAI-1 concentrations were significantly higher in PE pregnant women when compared with normotensive pregnant women. In PE pregnancy cfDNA was significantly and positively correlated with TNF-alpha, VCAM, tPA and PAI-1 levels. CfDNA levels were also positively correlated with the PE severity, estimated by proteinuria. Our data suggest that the associated inflammation and endothelial dysfunction in PE pregnancy seems to lead to an increase in cfDNA, and its levels seem to be a potential marker of PE severity. Thus, cfDNA might be a potential candidate in the noninvasive diagnostic field. Moreover, further studies are warranted to evaluate cfDNA value as a premature marker to the development of PE. C. Catarino: None. S. Coimbra: None.L. Belo:None. S. Rocha: None. M. Bicho: None. I. Rebelo: None. A. Santos-Silva: None. Copyright © 2014.
    01/2015; 5(1):70-1. DOI:10.1016/j.preghy.2014.10.140
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    ABSTRACT: Erythroid hypoplasia (EH) is a rare complication associated with recombinant human erythropoietin (rHuEPO) therapies, due to development of anti-rHuEPO antibodies; however, the underlying mechanisms remain poorly clarified. Our aim was to manage a rat model of antibody-mediated EH induced by rHuEPO and study the impact on iron metabolism and erythropoiesis. Wistar rats treated during 9 weeks with a high rHuEPO dose (200 IU) developed EH, as shown by anemia, reduced erythroblasts, reticulocytopenia, and plasmatic anti-rHuEPO antibodies. Serum iron was increased and associated with mRNA overexpression of hepatic hepcidin and other iron regulatory mediators and downregulation of matriptase-2; overexpression of divalent metal transporter 1 and ferroportin was observed in duodenum and liver. Decreased EPO expression was observed in kidney and liver, while EPO receptor was overexpressed in liver. Endogenous EPO levels were normal, suggesting that anti-rHuEPO antibodies blunted EPO function. Our results suggest that anti-rHuEPO antibodies inhibit erythropoiesis causing anemia. This leads to a serum iron increase, which seems to stimulate hepcidin expression despite no evidence of inflammation, thus suggesting iron as the key modulator of hepcidin synthesis. These findings might contribute to improving new therapeutic strategies against rHuEPO resistance and/or development of antibody-mediated EH in patients under rHuEPO therapy.
    BioMed Research International 12/2014; DOI:10.1155/2014/421304 · 2.71 Impact Factor
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    ABSTRACT: We evaluated the potential cardiovascular risk protection of bilirubin in hemodialysis (HD) patients. An enlarged set of studies were evaluated in 191 HD patients, including hematological study, lipid profile, iron metabolism, nutritional, inflammatory markers, and dialysis adequacy. The TA duplication screening in the UDP-glucuronosyltransferase 1 A1 (UGT1A1) promoter region was also performed. The UGT1A1 genotype frequencies in HD patients were 49.2%, 42.4%, and 8.4% for 6/6, 6/7, and 7/7 genotypes, respectively. Although no difference was found in UGT1A1 genotype distribution between the three tertiles of bilirubin, significant differences were found with increasing bilirubin levels, namely, a decrease in platelet, leukocyte, and lymphocyte counts, transferrin, oxidized low-density lipoprotein (ox-LDL), ox-LDL/low-density lipoprotein cholesterol ratio, apolipoprotein (Apo) A, Apo B, and interleukin-6 serum levels and a significant increased concentration of hemoglobin, hematocrit, erythrocyte count, iron, transferrin saturation, Apo A/Apo B ratio, adiponectin, and paraoxonase 1 serum levels. After adjustment for age these results remained significant. Our data suggest that higher bilirubin levels are associated with beneficial effects in HD patients, by improving lipid profile and reducing the inflammatory grade, which might contribute to increase in iron availability. These results suggest a potential cardiovascular risk protection of bilirubin in HD patients.
    BioMed Research International 09/2014; 2014:175286. DOI:10.1155/2014/175286 · 2.71 Impact Factor
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    ABSTRACT: Toll-like receptors (TLRs) play a key role in the response of innate and adaptive immune system to microbial and endogenous ligands. Inflammation is a common feature in end-stage renal disease (ESRD) patients; however, the mechanisms/factors triggering the inflammatory process are still poorly clarified. Our aim was to analyze the impact of the c.-1486T>C and c.896A>G polymorphisms in TLR9 and TLR4 genes, respectively, on the inflammatory response of ESRD patients. Clinical and laboratory evaluation was carried out on 184 ESRD patients. Polymerase chain reaction followed by restriction fragmens length polymorphisms (PCR-RFLP) was employed for genotyping of TLR-4 c.896A>G and TLR-9 c.-1486T>C polymorphisms. The prevalence of AA and AG of TLR4 c.896A>G polymorphism in ESRD patients was 97.8% and 2.2%, respectively. None of the individuals showed a homozygous TLR4 polymorphism. Concerning the TLR9 c.-1486T>C polymorphism, we found that ESRD patients showed a prevalence of TC and CC genotypes of 57.1% and 20.6%, respectively. We found that the heterozygous patients for the TLR4 c.896A>G polymorphism presented an increased level in lymphocyte count, a decrease in neutrophil/lymphocyte ratio and in serum levels of hepcidin. Regarding the TLR9 c.-1486T>C polymorphism, we found that it is associated with decreased white blood cell and neutrophil counts, ferritin and CRP serum levels, and with an increase in serum levels of creatinine. Our data suggest that the presence of the studied polymorphisms is associated with a decreased inflammatory response in ESRD patients under hemodialysis, and, thus its presence might have beneficial effects in ESRD patients. Moreover, our data provide new insights in the role of TLR polymorphisms in renal disease, which might have impact in the near future for the development of innovative therapies.
    European Journal of Inflammation 09/2014; 12(3):521-529. · 0.99 Impact Factor
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    ABSTRACT: Waldenström’s macroglobulinemia (WM) is a lymphoproliferative disease of B lymphocytes, characterized by a lymphoplasmocytic lymphoma in the bone mar-row and by IgM monoclonal hypergammaglobulinemia. It was first described in 1944 by Jan Gösta Waldenström, reporting two patients with oronasal bleeding, lymphadenopathy, anemia, thrombocytopenia, high erythrocyte sedimentation rate and serum viscosity, normal radiography and bone marrow infiltrated by lymphoid cells. The WM is a rare disease with a typically indolent clinical course, affecting mainly individuals aged between 63 and 68 years. Most patients have clinical signs and symptoms related to hyperviscosity resulting from IgM monoclonal gammopa-thy, and/or cytopenias resulting from bone marrow infiltration by lymphoma. The differential diagnosis with other lymphomas is essential for the assessment of prognosis and therapeutic approach. Treatment of patients with asymptomatic WM does not improve the quality of life of patients, or increase their survival, being recommended, therefore, their follow-up. For the treatment of symptomatic patients, alkylating agents, puri-ne analogs and anti-CD20 monoclonal antibodies are used. However, the disea-se is incurable and the response to therapy is not always favorable. Recent stu-dies have shown promising results with bortezomib, an inhibitor of proteasomes, and some patients respond to thalidomide. In patients with relapse or refrac-tory to therapy, autologous transplantation may be indicated. The aim of this paper is to describe in detail the current knowledge on the pa-thophysiology of WM, main clinical manifestations, diagnosis, prognosis and treatment.
    Revista da Associação Médica Brasileira 09/2014; 60(5):488-497. DOI:10.1590/1806-9282.60.05.019 · 0.92 Impact Factor
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    ABSTRACT: Olive leaf (OL) supplements are marketed as promoting health and supporting the body in preventing free radical damage. This study examined the effect of different concentrations of OL supplement on the haematological and lipid profile and on the oxidative stability of red blood cells (RBCs). A cohort of healthy pigs was used as a model in a single-centre, randomized, prospective pilot comparison. Twenty four pigs were assigned to three experimental diets: a control group fed the conventional diet and two groups fed the conventional diet supplemented at 50 and at 100 g/kg with OL, during 8 weeks. Blood was collected for haematological, biochemical, and haemostatic studies. OL supplementation resulted in a significant decrease in plasmatic triacylglycerols (TAGs) concentration, aligned with a lower body mass and fat storage but no significant reductions were found for low-density lipoprotein cholesterol (LDLc) and oxLDL levels. The use of the highest dose resulted in significant RBC membrane destabilization.
    Journal of Functional Foods 07/2014; 9(1):271–279. DOI:10.1016/j.jff.2014.04.027 · 4.48 Impact Factor
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    ABSTRACT: Objectives Bilirubin has potential antioxidant and anti-inflammatory properties. The UGT1A1*28 polymorphism (TA repeats in the promoter region) is a major determinant of bilirubin levels and recent evidence suggests that raised adiposity may also be a contributing factor. We aimed to study the interaction between UGT1A1 polymorphism, hematological and anthropometric variables with total bilirubin levels in young individuals. Methods 350 obese (mean age of 11.6 years; 52% females) and 79 controls (mean age of 10.5 years; 59% females) were included. Total bilirubin and C-reactive protein (CRP) plasma levels, hemogram, anthropometric data and UGT1A1 polymorphism were determined. In a subgroup of 74 obese and 40 controls body composition was analyzed by dual-energy X-ray absorptiometry. Results The UGT1A1 genotype frequencies were 49.9%, 42.7% and 7.5% for 6/6, 6/7 and 7/7 genotypes, respectively. Patients with 7/7 genotype presented the highest total bilirubin levels, followed by 6/7 and 6/6 genotypes. Compared to controls, obese patients presented higher erythrocyte count, hematocrit, hemoglobin and CRP levels, but no differences in bilirubin or in UGT1A1 genotype distribution. Body fat percentage was inversely correlated with bilirubin in obese patients but not in controls. This inverse association was observed either in 6/7 or 6/6 genotype obese patients. UGT1A1 polymorphism and body fat percentage were the main factors affecting bilirubin levels within obese patients (linear regression analysis). Conclusion In obese children and adolescents, body fat composition and UGT1A1 polymorphism are independent determinants of total bilirubin levels. Obese individuals with 6/6 UGT1A1 genotype and higher body fat mass may benefit from a closer clinical follow-up.
    PLoS ONE 06/2014; 9(6):e98467. DOI:10.1371/journal.pone.0098467 · 3.23 Impact Factor
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    ABSTRACT: Background:Adiponectin circulates as low, medium and high molecular weight multimers (LMW, MMW and HMW) and influences lipid profile and insulin resistance (IR), being HMW considered the most biological-active form. We aimed to study the relation between adiponectin and markers of metabolic syndrome, in paediatric obesity, and the impact of physical exercise.Methods:The study consisted of a cross-sectional part and an 8-months physical exercise program. Lipid profile, insulin, glucose, C-reactive protein (CRP), total adiponectin (TA), and homeostasis model assessment insulin resistance (HOMA-IR) were measured. Adiponectin multimers were studied in a pre-pubertal group.Results:Obesity associated with increased dyslipidaemia, IR and inflammation. TA correlated inversely with adiposity, triglycerides, HOMA-IR and CRP, and positively with HDLc/Total Cholesterol (TC) ratio. HMW mimicked TA associations. The intervention program led to a reduction of TC, LDLc, insulin, HOMA-IR and trunk percentage of fat, and an increase of HDLc/TC ratio, in the obese group. BMI improvements prevented adiponectin reduction and correlated with increments in HMW and MMW.Conclusions:Obesity-related increase in MS features might be linked to lower adiponectin. HMW and MMW were the multimers that most explained MS features. The intervention program improved the lipid profile and IR, and prevented the reduction of adiponectin.Pediatric Research (2014); doi:10.1038/pr.2014.73.
    Pediatric Research 05/2014; 76(2). DOI:10.1038/pr.2014.73 · 2.84 Impact Factor
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    ABSTRACT: Introduction and Aims: Both increased albuminuria and reduced kidney function predict blood pressure (BP) progression in the community, and exacerbate each other’s effects. We investigated associations and interactions between these two risk factors, BP changes and hypertension incidence in community-dwelling elderly men. Methods: Cross-sectional and longitudinal observational study in the Uppsala Longitudinal Study of Adult Men. 1051 men (all aged 71 years) with assessments on urinary albumin excretion rate (UAER, performed on an overnight urine collection.), 24-hour ambulatory BP monitoring (ABPM) and cystatin-C estimated glomerular filtration rate (eGFR). Of these, 574 men attended re-examination after 6 years, and ABPM measurements were again recorded. Results: UAER associated with ABPM measurements both at baseline and longitudinally. In longitudinal analysis, there were significant interactions between UAER and kidney function in their associations with changes of systolic BP, mean arterial pressure, and pulse pressure. After stratification for renal function state, UAER independently predicted BP changes only in those who had eGFR<60 mL/min/1.73m2. At re-examination, 71 new cases of hypertension were recorded. In multivariable logistic models of hypertension incidence, similar interactions were observed: UAER was an independent predictor of incident hypertension only in those with reduced renal function. These associations were evident also in the subpopulation of participants with normal range UAER (<20ug/min). Conclusions: UAER, even within the normal range, associates with BP progression and hypertension incidence in community-dwelling elderly men but only in those with concurrent reduction of renal function. View larger version: In this window In a new window Download as PowerPoint Slide
    Nephrology Dialysis Transplantation 05/2014; 29 Suppl 3(suppl 3):iii79-iii89. DOI:10.1093/ndt/gfu142 · 3.49 Impact Factor
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    ABSTRACT: Introduction and Aims: Hypertensive nephrosclerosis is one of the most frequent causes of chronic kidney failure. Proteomic analysis represents one possibility to improve the pathophysiologic knowledge and diagnostic precision of this disorder. In this study, we investigated experimental nephrosclerosis in two-kidney, one-clip (2K1C) hypertensive rats. Methods: The renal cortex proteome from juxtamedullary cortex (JMC) and outer cortex (OC) of 2K1C male Hannover Wistar rats (n=4) was compared to sham-operated controls (n=6) using mass spectrometry based quantitative proteomics. We combined a high abundant plasma protein depletion strategy with an extended liquid chromatographic gradient to improve peptide and protein identification. Immunohistology was used for confirmation of the abundance of a selected protein. Results: In total we identified 1,724 proteins, of which 1,434 could be quantified based on ≥ 2 unique peptides. Comparative proteomics revealed 683 proteins, including PDGFR-β pathway, with different abundance between the non-clipped renal cortex of hypertensive 2K1C rats and of the corresponding kidney in normotensive controls (p<0.05, absolute fold change ≥1.5). A total of 12 proteins were differentially regulated between JMC and OC of 2K1C animals. Among the most significantly altered proteins in the whole cortex, we identified periostin, transgelin and creatine kinase B-type with known association to renal fibrosis. Their relative abundance pattern separates 2K1C and controls based on a 3D scatter plot (Fig. 1A) and on different decision algorithms (Table 1). Also, the relative abundance of periostin alone indicated perfect classification, as assessed by a scatter plot (Fig. 1B). Enrichment of periostin was verified by immunohistology showing no periostin staining in control cortex (Fig. 2A) but clear positivity around fibrotic vessels in nephrosclerosis (Fig. 2B). Conclusions: The proteome is substantially altered in hypertension-induced kidney damage. We propose periostin and especially periostin in combination with transgelin and creatine kinase B-type as a possible proteomic classifier to distinguish hypertensive nephrosclerosis from normal tissue. This classifier needs to be further validated with respect of early fibrosis diagnosis, of prognosis and for its potential as a novel target. View larger version: In this window In a new window Download as PowerPoint Slide
    Nephrology Dialysis Transplantation 05/2014; 29(Suppl 3):ii201-iii208. DOI:10.1093/ndt/gfu152 · 3.49 Impact Factor
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    ABSTRACT: Our aim was to contribute to a better understanding of the pathophysiology of anemia in elderly, by studying how aging affects renal function, iron metabolism, erythropoiesis and the inflammatory response, using an experimental animal model. The study was performed in male Wistar, a group of young rats with 2 months age and an old one with 18 months age. Old rats presented a significant higher urea, creatinine, interferon (INF)-gamma, ferritin and soluble transferrin receptor serum levels, as well as increased counts of reticulocytes and RDW. In addition, these rats showed significant lower erythropoietin (EPO) and iron serum levels. Concerning gene expression of iron regulatory proteins, old rats presented significantly higher mRNA levels of hepcidin (Hamp), transferrin (TF), transferrin receptor 2 (TfR2) and hemojuvelin (HJV); divalent metal transporter 1 (DMT1) mRNA levels were significantly higher in duodenal tissue; EPO gene expression was significantly higher in liver and lower in kidney, and the expression of the EPOR was significantly higher in both liver and kidney. Our results showed that aging is associated with impaired renal function, which could be in turn related with the inflammatory process and with a decline in EPO renal production. Moreover, we also propose that aging may be associated with INF-gamma-induced inflammation and with alterations upon iron regulatory proteins gene expression.
    Aging and Disease 02/2014; 5(2). DOI:10.14366/AD.2014.0500356
  • British journal of biomedical science 01/2014; 71(2):79-81. · 0.83 Impact Factor

Publication Stats

1k Citations
246.32 Total Impact Points

Institutions

  • 2000–2015
    • University of Porto
      • • Departamento de Ciências Biológicas
      • • Institute for Molecular and Cell Biology
      • • Faculty of Pharmacy
      • • Department of Biochemistry
      Oporto, Porto, Portugal
  • 2008–2010
    • Universidade Católica Portuguesa
      • Instituto de Ciências da Saúde do Porto
      Lisboa, Lisbon, Portugal
  • 2000–2008
    • Institute for Molecular and Cell Biology
      Oporto, Porto, Portugal
  • 2005
    • Instituto de Biologia Molecular do Paraná
      Curityba, Paraná, Brazil