-
[show abstract]
[hide abstract]
ABSTRACT: Herein, folic acid (FA) conjugated with AuNPs were introduced into the cancer cell imaging via the specific interaction between FA and the folate receptor on the cell surface. FA protected gold nanoparticles (AuNPs) was synthesized and labeled with fluorescein isothiocynate (FITC) to form the FITC-FA-AuNPs (FFANPs). As over-expressed folic acid receptor in some cancer cells and folic acid can specifically and selectively combine, the FFANPs can bind to the FR expressed on tumor cells such as HeLa cells (human epithelial cervical cancer) and CERF-CEM cells (T cell line, human acute lymphoblastic leukemia). As a result, cancer cell imaging can be achieved. To ascertain the FR target ability, it has been acquired by FR-targeted images using synthetic FFANPs. The formation of FITC-FA can be identified by MS. FCM was carried out to study the cell uptake of FFANPs. The cell toxicity (3-[4,5-dimethylthiazolyl-2]-2, 5-diphenyltetrazolium bromide, MTT) assay demonstrated that the FITC-labeled conjugate had only little effect on the cytotoxicity to the cells, which further proved the applicability of the method in tumor cell imaging.
Talanta 11/2012; 101:32-7. · 3.79 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this article, we reported a novel approach for in situ labeling and imaging HeLa cancer cells utilizing a bifunctional aptamer (AS1411) and its fluorescent ligand, protoporphyrin IX (PPIX). In the presence of potassium ion, AS1411 folded to G-quadruplex structure, binded fluorescent ligand (PPIX) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. Consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. The bioimaging strategy with AS1411-PPIX complex was capable to distinguish HeLa cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. Moreover, the bioimaging method was very facile, effective and need not any covalent modification. These results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis.
Analytica chimica acta 09/2012; 741:93-9. · 4.31 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: An effective G-quadruplex-based probe has been constructed for rapid and sensitive detection of Cu(2+). In this probe, an anionic porphyrin, protoporphyrin IX (PPIX) served as a reference signal, which binds to G-quadruplex specifically and the fluorescence intensity increases sharply. While, in the presence of Cu(2+), the G-quadruplex can catalyze the related Cu(2+) insertion into the protoporphyrin, the fluorescent intensity is decreased. The fluorescence of the response ligand could be selectively quenched in the presence of Cu(2+) and not interfered by other metal ions. The probe provided an effective platform for reliable detection of Cu(2+) with a detection limit as low as 3.0nM, the high sensitivity was attributed to the strong metalation of PPIX with Cu(2+) catalyzed by G-quadruplex (PS5.M). Linear correlations were obtained over the logarithm of copper ion concentration in the range from 8×10(-9)M to 2×10(-6)M (R=0.998). The G-quadruplex-based probe also could be used to detect Cu(2+) in real water samples. Additionally, these striking properties endow the G-quadruplex-ligand with a great promise for analytical applications.
Biosensors & bioelectronics 08/2012; 39(1):268-73. · 5.43 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A co-precipitation method was employed to prepare Ni/Al2O3-ZrO2, Co/Al2 O3-ZrO2 and Ni-Co/Al2O3-ZrO2 catalysts. Their properties were characterized by N2 adsorption (BET), thermogravimetric analysis (TGA), temperature-programmed reduction (TPR), temperature-programmed desorption
(CO2-TPD), and temperature-programmed surface reaction (CH4-TPSR and CO2-TPSR). Ni-Co/Al2O3-ZrO2 bimetallic catalyst has good performance in the reduction of active components Ni, Co and CO2 adsorption. Compared with mono-metallic catalyst, bimetallic catalyst could provide more active sites and CO2 adsorption sites (C + CO2 = 2CO) for the methane-reforming reaction, and a more appropriate force formed between active components and composite support
(SMSI) for the catalytic reaction. According to the CH4-CO2-TPSR, there were 80.9% and 81.5% higher CH4 and CO2 conversion over Ni-Co/Al2O3-ZrO2 catalyst, and its better resistance to carbon deposition, less than 0.5% of coke after 4 h reaction, was found by TGA. The
high activity and excellent anti-coking of the Ni-Co/Al2O3-ZrO2 catalyst were closely related to the synergy between Ni and Co active metal, the strong metal-support interaction and the
use of composite support.
KeywordsNi-Co bimetallic catalyst-composite support-CH4 reforming with CO2
Frontiers of Chemical Engineering in China 04/2012; 4(4):476-480.
-
[show abstract]
[hide abstract]
ABSTRACT: To establish a quick method for evaluation of the antioxidant activities based on the correlation analysis of lignans content and antioxidant activities of Schisandra chinensis fruits.
The content of five lignans components in 37 batches of S. chinensis fruits from different regions of Jilin province were measured by HPLC. Simultaneously, the antioxidant activities of the above samples were detected, such as lipid peroxidation inhibition activity in liver (LPIL), kidney (LPIK) and brain (LPIB) and the clearance rate of DPPH (CRD). Bivariate correlation analysis and stepwise regression analysis were carried out by the software of SPSS for windows 11.5.
The results of bivariate correlation analysis showed that deoxyschizandrin was negative correlation (P<0.01) to the activity of LPIL, LPIB, CRD. Schisandrin was positive correlation (P<0.01) to the activity of LPIL, LPIB, CRD. Schisandrol B was also positive correlation (P<0.05 or P<0.01) to the above four kinds of antioxidant activity. The results of stepwise regression analysis were mostly consistent with the bivariate correlation analysis results. For the other 10 batches of samples, the simulated antioxidant activities according to the regression equation calculated was consistent with the measured activities.
By using the bivariate correlation analysis and linear stepwise regression analysis, the bioactive components related to the antioxidant activity of S. chinensis fruits were found. Meanwhile, the antioxidant activity of samples will be inferred according to the content of Schisandra lignans.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 04/2012; 37(8):1133-9.
-
[show abstract]
[hide abstract]
ABSTRACT: Herein, a novel kind of silver nanocluster is synthesized simply by mixing G-quadruplex template with silver ions and reduction reagent (NaBH(4), here). AS1411 (a G-quadruplex that can bind nucleolin overexpressed in cancer cells) is used as the main model template to prove the synthesis protocol and its potential application. We used fluorescence assay, CD, MALDI TOF MS, and TEM to characterize the silver nanocluster. It is found that after formation of the silver nanocluster, AS1411 still keeps its structure and is able to bind with nucleolin in cancer cell. Meanwhile, this binding behavior can greatly enhance the fluorescence intensity of the silver nanocluster. This property can be directly employed into bioimaging HeLa cells. The cell toxicity (3-[4,5-dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide, MTT) assay demonstrated that the silver nanocluster has only little affect on the cytotoxicity to the cells, which further proves the applicability of the method in tumor cell imaging. At last, the universality of the synthesis protocol is verified by using a series of other G-quadruplex sequences as templates. For a lot of functional nucleic acids, such as human telomeres and certain aptamers, are with G-rich sequences and can fold into G-quadruplexes in functioning conditions, our method displays a promising application space in future researches.
Talanta 01/2012; 88:450-5. · 3.79 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Electrospray ionization ion trap multiple-stage tandem mass spectrometry (ESI-MS(n)) was used to evaluate Fructus Schisandrae of similar species (Schisandra chinensis (Turcz.) Baill. fruits and Schisandra sphenanthera Rehd. et Wils. fruits) and different growth characteristics (color, shape, etc.). The application of chemical pattern recognition in the ESI-MS(n) data analysis was carried out by principal component analysis (PCA), hierarchical cluster analysis (HCA) and linear discriminant analysis (LDA). Then the antioxidant activity of different Fructus Schisandrae samples were determined by an LC-ESI-MS method and ferric reducing antioxidant power (FRAP) assay. Using the ESI-MS(n) method coupled with chemical pattern recognition analysis and correlated with the antioxidant activity evaluation, the two similar species were successfully distinguished, thus improving the therapeutic safety and effectiveness. The superior characteristics of Schisandra chinensis (Turcz.) Baill. fruits were obtained and made the selection and breeding of Chinese medicine materials more scientific. This study indicates that ESI-MS(n) is a valuable tool for the authentication of botanical origin and can also be useful for the quality control of Chinese medicinal herbs.
The Analyst 09/2011; 136(20):4308-15. · 4.23 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A novel kind of versatile logic device has been constructed utilizing ion-tuned DNA/Ag fluorescent nanoclusters, with K(+) and H(+) as two inputs. A well-chosen hairpin DNA with a poly-C loop serves as the template for synthesizing two species of Ag nanoclusters. Several G-tracts and C-tracts on its two terminals enable the hairpin DNA to convert into the G-quadruplex and/or i-motif structures upon input of K(+) and H(+). Such a structural change remarkably influences the spectral behaviors of Ag nanoclusters. In particular, different species of Ag nanoclusters have distinct fluorescence responses to the input of K(+) and H(+). These unique features of DNA/Ag nanoclusters enable multiple logic operations via multichannel fluorescence output, indicating the versatility as a molecular logic device. By altering the specific sequence of the hairpin DNA, more logic gates can be constructed utilizing Ag nanoclusters.
ACS Nano 08/2011; 5(8):6334-8. · 10.77 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Currently, no satisfactory biomarkers are available to screen for esophageal squamous cell carcinoma (ESCC). The goal of this study was to find biomarkers and establish a serum protein fingerprint model for early diagnosis of ESCC using the ClinProt protocol of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Serum samples were collected from 62 patients with ESCC, nine patients with esophageal adenocarcinoma (EA) and 38 healthy individuals. Proteomic spectra of mass to charge ratio (m/z) were generated following the application of plasma to weak cationic-exchanger magnetic beads (WCX-MB). The spectral data were analyzed using a support vector machine, and potential biomarkers were chosen for system training and used to construct diagnostic models.
Three differential patterns were established using MALDI-TOF MS. Pattern 1, consisting of 11 protein peaks, separated ESCC patients from the healthy individuals with a sensitivity of 90.0% and a specificity of 88.4%. Pattern 2, consisting of eight protein peaks, separated ESCC in stage I and stage II from stage III and stage IV with a sensitivity of 92.9% and a specificity of 82.3%. Pattern 3, consisting of seven protein peaks, separated ESCC from EA with a sensitivity of 91.3% and a specificity of 80.0%.
These results suggested that MALDI-TOF MS combined with MB separation yields significantly higher sensitivity and specificity for the detection of serum protein in patients with ESCC.
Clinical Chemistry and Laboratory Medicine 03/2010; 48(6):855-61. · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Software test data generation is an important part of software testing. This paper presents a multi-agent cooperation framework for software test data generation. The framework is constituted by Graph Miner, Method Selector and test data generation method agent group. Graph Miner extracts software information sequences from UML graphs, and sends them to Method Selector. Then Method Selector selects out relevant method agents to generate test data. This framework can solve the problems of test data generation methods extension and low level of intelligence exist in traditional methods. Based on the framework proposed in this paper, a software prototype is developed. It is proved that this framework is feasible.
International Journal of Software Engineering and Its Applications. 02/2010; 4.
-
[show abstract]
[hide abstract]
ABSTRACT: Study the immunoregulatory function of rat bone marrow mesenchymal stem cells on spleen mononuclearcell (MNC) in vitro, and investigate the immunological regulation mechanism initially.
MSCs from bone marrow of rat were isolated and cultured in vitro. The morphological charactcrization of MSCs was observed after Wright-Giemsa's staining and the typical molecule on the cell surface were identified by flow cytometry(FCM). ConA was taken as stimuli origin. Effect of MSCs on proliferation of spleen MNC was determined by MTT method.Effect of MSCs on IL-2 and IL-10 secretion of spleen MNC was determined by ELISA.Analyze the effect of MSCs on MNC cell cycle distribution by FCM.Study the effect of MSCs on p27 and cyclin E expression of MNC by FCM. Investigate the effect of MSCs on spleen MNC killing colon26 and H22 by LDH release method.
Compared with positive control, proliferation of spleen MNC decreased obviously, when cultured with different number of MSCs (P<0.01), and the proliferation of MNC was inhibited markedly in a dose-dependent manner (P<0.01). After treated by MSCs, the level of IL-2 secreted by spleen MNC decreased, but the level of IL-10 increased markedly (P<0.01), and this function was also in a dose-dependent manner. MSCs could arrest spleen MNC to G(0);/G(1); phase (P<0.01), and inhibit spleen MNC entering S phase, when stimulated by ConA. MSCs could upregulate p27 expression and downregulate cyclin E expression of spleen MNC (P<0.05).After culturing with MSCs, ability of spleen MNC killing colon26 and H22 was impaired, compared with group cultured alone (P<0.05).
MSCs could inhibit spleen MNC proliferation in vitro, and this effect was related to that MSCs could alter cytokine secretion and regulate cell cycle distribution of MNC.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 12/2009; 25(12):1119-22.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the immune mechanisms for Periplocin from Cortex Periplocae (CPP) in tumor-bearing mice.
H(22) tumor-bearing model BALB/c mice were applied to evaluated in vivo immunoregulatory effect of CPP. The influence of different dose CPP (0.25, 0.50 and 1.00 mg/kg) on immune organs in tumor-bearing mice were observed. T cell subsets of mice spleen were detected by flow cytometry. MTT assay was used to determine the influence of CPP on lymphocyte proliferation of mice spleen stimulated by ConA. The levels of TNF-alpha, IL-2 and IL-12 in serum from mice were detected by means of ELISA.
Thymus index and spleen index of H(22) tumor-bearing model control mice became less than that of normal mice (P<0.05). Compared to both model and normal control groups, thymus index and spleen index of H(22) tumor-bearing mice treated with CPP increased obviously (P<0.05). CPP had no influence on the number of CD8(+) T cells, but up-regulated markedly the number of CD3(+), CD4(+) T cells and the ratio of CD4(+)/CD8(+) in tumor-bearing mice. In CPP-treated mice, the percentage of CD3(+), CD4(+) T cells were not different from normal mice (P<0.05), the ratio of CD4(+)/CD8(+) was higher than that of normal mice (P<0.05). CPP enhanced obviously lymphocyte proliferation of mice spleen induced by ConA, the SI scores were even higher than that of normal mice. The levels of TNF-alpha, IL-2 and IL-12 in serum from CPP-treated mice, increased significantly compared to model control group (P<0.05) in a dose-dependent manner, were similar to or higher than that of normal mice.
CPP protected immune organs of tumor-bearing mice, increased obviously the percentage of CD4(+) and CD4(+)/CD8(+) among the T cell line, and enhanced lymphocyte proliferation of mice spleen significantly, stimulated the production of TNF-alpha, IL-2 and IL-12. The results suggested that CPP possessed potent immunoregulatory effect.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 10/2009; 25(10):887-90.
-
[show abstract]
[hide abstract]
ABSTRACT: To objectively evaluate the clinical effect of traditional Chinese medicine in treating children's respiratory syncytial viral pneumonia (RSVP) of phlegm-heat blocking Fei syndrome (PHBFS).
A single-blinded multi-center, blocked, randomized and parallel-controlled method was adopted. The clinical study was carried out on 206 children with RSVP-PHBFS who were assigned to two groups, 108 in the test group treated through intravenous dripping of Qingkailing Injection () in combination of oral intake of Er'tong Qingfei Oral Liquid () and 98 in the control group with intravenous dripping of ribavirin injection in combination with oral intake of potassium guaiacol sulfonate oral liquid, all for 10 days. The clinical efficacy was evaluated and compared at the end of the trial from various aspects by three methods including comprehensive efficacy, post-treatment main symptoms score difference and survival analysis of the main symptoms.
After treatment, in the test group, 60 patients were cured, 36 markedly alleviated, and 12 improved. In the control group, 41 were cured, 38 markedly alleviated, 18 improved and 1 unchanged. Comparison on the comprehensive efficacy between the two groups shows a better efficacy in the test group (chi(2)=4.4527, P=0.0348). Scores of the main symptoms were lowered after treatment in both groups, the difference was 22.41+/-4.99 scores in the test group and 17.61+/-6.34 scores in the control group, being more significant in the former (t=-5.99, P<0.01). Survival analysis shows that there was significant difference between the two groups in the effect initiating time on such symptoms as fever, cough, copious sputum, shortness of breath, and rales, which was earlier in the test group (P<0.01 or P<0.05).
Evaluation of the efficacy of traditional Chinese medicine in treating children with RSVP-PHBFS by using the three methods jointly could better show the objectivity of the evaluation.
Chinese Journal of Integrative Medicine 04/2009; 15(2):95-100. · 0.80 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Cancer occurrence and development has been demonstrated to be associated with escape from immune surveillance, and low costimulatory molecules expression has been considered as one of the important reasons for cancer evading the immune system. 4-1BB (CD137) is a costimulatory molecule expressed on the surface of activated T cells. Interaction of 4-1BB with its natural ligand (4-1BBL) expressed on antigen presenting cells (APCs) has been shown to amplify T-cell mediated immunity. We therefore examined whether murine cancer cells expressing 4-1BBL could produce anti-tumor effects in inoculated mice. Mouse forestomach carcinoma (MFC) cells were transfected with 4-1BBL gene (MFC/4-1BBL). The proliferation of the transduced cells in vitro was not different from that of parental cells. However, MFC/4-1BBL cells developed small tumors and induced higher cytotoxicity of tumor infiltration lymphocyte (TIL). Production of cytokines (IFN-gamma, TNF-alpha and IL-2) in serum and cytotoxic T lymphocyte (CTL) activity of splenocytes from mice immunized with mitomycin C (MMC)-treated MFC/4-1BBL cells were significantly higher than that from mice immunized with MMC-treated parental MFC and MFC/pMKITneo cells. These results suggest that modification of cancer cells with 4-1BBL gene can produce anti-tumor immune responses.
Cellular & molecular immunology 11/2008; 5(5):379-84. · 2.99 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To study the effect of Zheng's plant-protein(ZPP) and phytohemagglutinin(PHA) on proliferation of peripheral blood lymphocytes (PBL) and it's application value on the early diagnosis and therapy of breast cancer.
The proliferation assay was used (3)H-TdR incorporation test. The phenotypes of PBL were analysised by flow cytometry(FCM).
The ZPP significantly and selectively enhanced proliferation activity of PBL of primary breast and precancer cases. Their stimulation indexes(SI) were 3.00+/-1.45 and 2.53+/-0.80, while the SI of simple hyperplasia cases and normal subjecties were 1.18+/-0.47 and 1.06+/-0.91. The former were significantly higher than the latter (P<0.01). The ZPP was also enhanced the expression level of CD3(+),CD4(+) and CD8(+) of PBL.
This results suggest that ZPP might be a beast cancer common antigen. These activity might be used in clinical implication to diagnosis and treatment of breast cancer.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 04/2003; 19(2):138-9.
-
[show abstract]
[hide abstract]
ABSTRACT: Similarity between the roles of the group on the equation for self-similar solutions of the anisotropic affine curve shortening problem and of the conformal group
of on the Nirenberg problem for prescribed scalar curvature is explored. Sufficient conditions for the existence of affine self-similar
curves are established.
Calculus of Variations 10/2001; 13(3):311-337. · 1.24 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Software test data generation is an important part of software testing. This paper presents a multi-agent cooperation framework
for software test data generation. The framework is constituted by Graph Miner, Method Selector and test data generation method
agent group. Graph Miner extracts software information sequences from UML graphs, and sends them to Method Selector. Then
Method Selector selects out relevant method agents to generate test data. This framework can solve the problems of test data
generation methods extension and low level of intelligence exists in traditional methods. Based on the framework proposed
in this paper, a software prototype is developed. It is proved that this framework is feasible.
01/1970: pages 188-195;
