S Zani

Università degli Studi di Siena, Siena, Tuscany, Italy

Are you S Zani?

Claim your profile

Publications (7)15.77 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Intraerythrocyte nonprotein-bound iron (INPBI), malondialdehyde (MDA), and hypoxanthine plasma levels (HxPL), were determined by high-pressure liquid chromatography in 138 randomly selected newborn infants with gestational ages ranging from 23 to 42 weeks at birth and on fourth day of life. MDA plasma levels were significantly higher in cord and fourth-day blood samples of preterm babies than term infants as well as babies born by emergency Caesarean section than babies born by vaginal delivery and in intubated than in nonintubated newborns. Highly significant correlations both in cord blood and fourth-day blood samples were observed between MDA plasma levels and gestational age, birth weight, Apgar score at 1 min and 5 min, HxPL, pH, base deficit, and INPBI content. Multiple regression analysis identified HxPL as the best single predictor of MDA plasma levels in cord blood, and INPBI content in fourth-day blood as the best single predictor of MDA plasma levels in fourth-day blood. The results indicate that red cells and plasma lipoproteins are a common target of free radical-induced oxidative stress during hypoxia.
    Free Radical Biology and Medicine 12/1998; 25(7):766-70. · 5.27 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Heparinized blood samples were obtained at birth from 164 newborn infants (101 full term; 63 preterm). Intra-erythrocyte free iron concentration and hypoxanthine plasma levels were determined by high-pressure liquid chromatography. Intra-erythrocyte free iron concentration was higher in preterm than in full term babies (p < 0.0001) and adults (p < 0.0001). Statistically significant correlations were observed between intra-erythrocyte free iron concentration and hypoxanthine levels (r = 0.66; p = 0.0001), pH (r = -0.76; p = 0.0001), base excess (r = -0.79; p = 0.0001), and gestational age (r = -0.44; p = 0.0001) in both infant populations. Multiple regression analysis between intra-erythrocyte free iron concentration in cord blood, as an independent variable, and Apgar score at 1 min, pH, base excess, hypoxanthine values, FiO2 needed for resuscitation immediately after delivery, and gestational age, as dependent variables, identified hypoxanthine levels (p = 0.0003; partial F-test = 15.4) as the best single predictor of intra-erythrocyte free iron concentration. In conclusion, hypoxia induces intra-erythrocyte free iron release, and therefore enhances the risk of oxidative injury due to hydroxyl radical generation.
    Acta Paediatrica 01/1998; 87(1):77-81. · 1.97 Impact Factor
  • Pediatric Research - PEDIAT RES. 01/1996; 39.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new method of computerized cry analysis has been utilized to evaluate the cries of infants affected by severe protein energy malnutrition. We studied 17 Kenian babies affected by severe malnutrition for more than four months (9 cases of marasmus and 8 of kwashiorkor) and a control group of 17 well-nourished babies. The cries of the malnourished children showed lower inter-utterance variability, formants' frequencies and cry score, assigned by the Infant Cry Modulation Assessment Scale. The melodic pattern was more often flat, rising or falling-rising, when compared to the cries of the well-nourished babies. We hypothesize that these differences reflect the state of brain damage associated with protein energy malnutrition. No differences were found between the cries of infants affected by marasmus and those affected by kwashiorkor, between the cries recorded before and after nutritional therapy and between the first cries of malnourished children who subsequently died during hospitalization and those of infants who survived.
    Acta Paediatrica 03/1994; 83(2):204-11. · 1.97 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new method of computerized cry analysis has been utilized to evaluate the cries of infants affected by severe protein energy malnutrition. We studied 17 Kenian babies affected by severe malnutrition for more than four months (9 cases of marasmus and 8 of kwashiorkor) and a control group of 17 well-nourished babies. The cries of the malnourished children showed lower inter-utterance variability, formants' frequencies and cry score. assigned by the Infant Cry Modulation Assessment Scale. The melodic pattern was more often flat, rising or falling-rising, when compared to the cries of the well-nourished babies. We hypothesize that these differences reflect the state of brain damage associated with protein energy malnutrition. No differences were found between the cries of infants affected by marasmus and those affected by kwashiorkor, between the cries recorded before and after nutritional therapy and between the first cries of malnourished children who subsequently died during hospitalization and those of infants who survived.
    Acta Paediatrica 01/1994; 83(2):204 - 211. · 1.97 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Increasing appreciation of the causative role of oxidative injury in the development of many severe diseases of the newborn places great importance on the reliable assessment of lipid peroxidation. Lipid peroxidation in the erythrocyte membrane has been widely investigated but little has been reported on lipid peroxidation in the plasma The aim of the present paper was to evaluate lipid peroxidation and peroxidation tissue injury in the plasma of newborns using the concentration of Malondialdehyde (MDA). one of the several substances formed when lipid hydroperoxides break down in biological systems Twenty-one, healthy, full-term newborn infants, (10 male and 11 female), with an Apgar score of more than 8 at 1 min, were studied. Fifteen were born by vaginal delivery (VD) and 6 by elective cesaerean section (ECS) Hepannized blood samples were taken at birth from the cord blood and on the 4th day of life from a peripheral vein MDA levels were determined by fluorometric assay using the MDA Kit (Sobioda, Grenoble France) We found significantly lower concentrations of MDA at birth (2 81±1 34 nmol/mL. meant±SD) than on 4th day of life (4.35±1.98. P<0 0003) in total population. This difference was also observed in the two mode of delivery when considered separately. No statistically significant differences were found between the two groups nor between the sexes. These results clearly demonstrate a significant increase in lipid peroxidation after birth. We speculate that environmental changes in O2 concentration from fetal to postnatal life are sufficient to produce an increase in aerobic metabolism and in the rate of free radical generation The deficient antioxidant mechanisms at birth could impair normal fetal equilibrium between oxygen free radical generation and free radical detoxification
    Pediatric Research 01/1994; 35(2). · 2.67 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the present study was to evaluate the IgE-mediated response as a predictable index of immunosensitization to different types of feeding (breast milk, adapted formula, soya formula and serum protein hydrolyzate formula) in the first days of life. The study population included 231 newborns (128 males and 103 females). There was a family history of atopy (at least 1 parent with an atopic disease) in 116 subjects at risk for allergy. 115 newborns were without any family history of atopy. The results showed significantly higher total IgE levels on the 4th day than in cord serum in the whole group of newborns. The same result was obtained comparing subjects at risk and controls separately. No significant difference was detectable between serum IgE levels in the cord blood of the four groups. Conversely, a significant increase in IgE levels between cord and 4th day blood in soya-fed and adapted-formula-fed babies was noticed. This increase did not occur in neonates fed breast milk and serum protein hydrolyzate.
    Biology of the Neonate 02/1992; 62(1):10-4. · 1.90 Impact Factor