Kazuko Takada

Nihon University, Edo, Tōkyō, Japan

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Publications (48)96.98 Total impact

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    ABSTRACT: Two strains were isolated from the oral cavity samples of healthy elephants. The isolates were gram-positive, catalase-negative, coccus-shaped organisms that were tentatively identified as a streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequence analysis classified these organisms into the genus Streptococcus with Streptococcus criceti ATCC 19642(T) and Streptococcus orisuis NUM 1001(T) as their closest phylogenetic neighbor with 98.2 and 96.9% similarity, respectively. When multilocus sequence analysis using four housekeeping genes, groEL, rpoB, gyrB and sodA, was carried out, percent homology of concatenated sequence of four housekeeping genes from new isolates and S. mutans showed 89.7%. DNA-DNA hybridization experiments suggested that isolates were distinct from S. criceti and other streptococcus species. On the basis of genotypic and phenotypic differences, it is proposed that the novel isolates are classified in the genus Streptococcus as Streptococcus oriloxodontae sp. nov. The type strain of S. oriloxodontae is NUM 2101(T) (=JCM 19285(T) =DSM 27377(T)).
    International journal of systematic and evolutionary microbiology. 08/2014;
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    ABSTRACT: Four Gram-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. The isolates were tentatively identified as a streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene-sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two isolates were related most closely to Streptococcus salivarius with 96.8% and 93.1% similarity based on the 16S rRNA gene and the RNA polymerase β subunit encoding gene (rpoB) gene, respectively, and to Streptococcus vestibularis with 83.7% similarity based on the 60 kDa heat-shock protein gene (groEL). The other isolates were related most closely to S. vestibularis with 97.0% similarity based on the 16S rRNA gene and to S. salivarius with 93.5% and 82.9% similarity based on the rpoB and groEL genes, respectively. Based on phylogenetic and phenotypic evidence, these isolates are suggested to represent a novel species of the genus Streptococcus. The names proposed for these organisms are Streptococcus loxodontisalivarius sp. nov. (type strain NUM 6304(T)= JCM 19287(T)= DSM 27382(T)) and Streptococcus saliviloxodontae sp. nov. (type strain NUM 6306(T)= JCM 19288(T)= DSM 27513(T)).
    International journal of systematic and evolutionary microbiology. 07/2014;
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    ABSTRACT: Corynebacterium matruchotii is a microbial inhabitant in the oral cavity of humans and is associated with the formation of dental calculi. C. matruchotii forms highly specific morphological units, which are referred to as corn-cobs. Although other Corynebacterium species have frequently been isolated from the oral cavity of humans, their distribution has not been reported as extensively. The aim of the present study was to develop a selective medium to isolate the genus Corynebacterium and examine the distribution Corynebacterium species in the oral cavity of humans. The growth recoveries of representative Corynebacterium species on the selective medium were sufficient. Moreover, the growth of other representative oral bacteria was markedly inhibited on the selective medium. The proportion of Corynebacterium species in saliva samples collected from 20 subjects was examined. PCR primers were designed for the oral Corynebacterium species. C. matruchotii and Corynebacterium durum accounted for 0.3 % and 1.5 % of the total cultivable bacteria number on the BHI medium from saliva samples, respectively. The selective medium could distinguish C. matruchotii from C. durum by each colony color using differences in acid production from galactose. The selective medium, designated OCM, was useful for isolating oral Corynebacterium species.
    Journal of microbiological methods. 06/2014;
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    ABSTRACT: Aggregatibacter actinomycetemcomitans is an important pathogen related to aggressively progressive periodontal breakdown in adolescents and adults. The species can be divided into six serotypes (a-f) according to their surface carbohydrate antigens. Recently, a new serotype g of A. actinomycetemcomitans was proposed. The aim of the present study was to sequence the gene cluster associated with the biosynthesis of the serotype g-specific polysaccharide antigen and develop serotype-specific primers for PCR assay to identify serotype g strains of A. actinomycetemcomitans. The serotype-specific polysaccharide (SSPS) gene cluster of the NUM-Aa 4039 strain contained 21 genes in 21,842-bp nucleotides. The similarity of the SSPS gene cluster sequence was 96.7 % compared with that of the serotype e strain. Seventeen serotype g genes showed more than 90 % homology both in nucleotide and amino acids to the serotype e strain. Three additional genes with 1,579 bp in NUM-Aa 4039 were inserted into the corresponding ORF13 of the serotype e strain. The serotype g-specific primers were designed from the insertion region of NUM-Aa 4039. Serotypes of the a-f strains were not amplified by serotype-specific g primers; only NUM-Aa 4039 showed an amplicon band. The NUM-Aa 4039 strain was three genes in the SSPS gene cluster different from those of serotype e strain. The specific primers derived from these different regions are useful for identification and distribution of serotype g strain among A. actinomycetemcomitans from clinical samples.
    Archives of Microbiology 02/2014; · 1.91 Impact Factor
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    ABSTRACT: Two tandemly aligned glucosyltransferase (GTF) genes whose gene products are responsible for water-soluble glucan synthesis were isolated from Streptococcus dentirousetti NUM1303 and sequenced. One of the GTF genes of S. dentirousetti consisted of a 4110 bp open reading frame (ORF) that encoded for a 1369 amino acid protein and was revealed to be a S. sobrinus gtfS homolog. The percent similarity of amino acid sequences of the GTF-S from S. dentirousetti compared to those from S. sobrinus was 99%. In addition, a putative gtfT was found in tandem in the downstream region of the S. dentirousetti gtfS. The gtfT of S. dentirousetti consisted of a 4527 bp ORF encoding for 1508 amino acids. The similarity of amino acid sequences of the GTF-T from S. dentirousetti and S. sobrinus was 94%. Phylogenetic analysis based on amino acid sequences from other related streptococcal GTFs suggested that both GTF-S and GTF-T of S. dentirousetti are closely related to S. sobrinus.
    Microbiology and Immunology 05/2013; 57(5):386-90. · 1.55 Impact Factor
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    ABSTRACT: Four Gram-positive, catalase-negative, coccoid-shaped isolates which were obtained from donkey oral cavities formed two distinct clonal groups when characterized by phenotypic and phylogenetic studies. From the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two of the isolates were related most closely to Streptococcus ursoris with 95.6% similarity based on the 16S rRNA gene and to Streptococcus ratti with 92.0% similarity based on the 60 kDa heat-shock protein gene (groEL). The other two isolates, however, were related to Streptococcus criceti with 95.0% and 89.0% similarities based on the 16S rRNA and groEL genes, respectively. From both phylogenetic and phenotypic evidence, the four isolates formed two distinct clonal groups and are suggested to represent a novel species of the genus Streptococcus. The names proposed for these organisms are Streptococcus orisasini sp. nov. ( type strain NUM 1801(T) = JCM 17942(T) = DMS 25193(T)) and Streptococcus dentasini sp. nov. (type strain NUM 1808(T) = JCM 17943(T) = DMS 25137(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
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    ABSTRACT: Objective The banding pattern of Streptococcus mutans strains generated using repetitive extragenic palindromic PCR (rep-PCR) with enterobacterial repetitive intergenic consensus (ERIC) primers has been shown previously. In the present study, the sequencing and analysis of gene sequences of the 6 most intense and commonly occurring amplicons from S. mutans serotype c was investigated. Methods The sequences of the amplicons from S. mutans serotype c strain by using rep-PCR with ERIC1R and ERIC2 primers were compared to the whole genome sequence of S. mutans UA159 and NN2025 strains. Results The amplicons were found to contain partial gene sequences coding for proteins such as the hypothetical protein, glucan binding protein A, putative methylated-DNA-protein-cysteine S-methyltransferase, putative d-3-phosphoglycerate dehydrogenase, putative excinuclease ABC (subunit A) and putative GTP-binding protein. The locations of 5 of the 6 amplicons were found to be assembled downstream in the UA159 genome. The coding direction of the amplicons in the NN2025 genome was in reverse orientation relative to that in the UA159 strain, except in the 2170-bp amplicon. Conclusions The repeating sequence elements of ERIC in S. mutans serotype c are located on one side of the genome and have less frequency and similarity compared to those in enterobacteria.
    Journal of Oral Biosciences 01/2013; 55(3):155–158.
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    ABSTRACT: Streptococcus mutans has been considered the principal etiological agent of dental caries in humans. S. mutans can secrete three kinds of glucosyltransferases (GTFs). One of these, GTF-B, which synthesizes water-insoluble glucans from sucrose, has been considered to be one of the most important factors of cariogenic dental plaque formation. Therefore, determination of whether GTF-B is present in plaque and saliva samples may contribute to the evaluation of individual virulence potential (caries risk). The aim of this study was to develop a feasible enzyme-linked immunosorbent assay (ELISA) for the routine quantification of GTF-B in plaque-derived cultures and clinical samples, and to apply this assay to an epidemiological study. To determine the presence of GTF-B in plaque samples, a sandwich-ELISA was devised, consisting of mouse monoclonal and rabbit polyclonal antibodies against GTF-B and a horseradish peroxidase-conjugated anti-rabbit antibody. The developed ELISA allowed for quantification of the amounts of purified GTF-B with satisfactory sensitivity and specificity; this method was not affected by other components such as plaque and saliva. Plaque samples from healthy volunteers were examined using this ELISA method and microbial analysis to apply the assay to an epidemiological study. A correlation was observed between the amount of extracted GTF-B and S. mutans levels as determined by ELISA and cultivated with Mitis Salivarius Bacitracin agar plates derived from plaque samples, although there were some exceptions. In this regard, this ELISA system has the advantage of estimating both the individual numbers of S. mutans and the productivity of GTF-B, namely, the cariogenic potential of S. mutans simultaneously. These results indicate that this ELISA method is a useful tool for the diagnosis of caries risk.
    Hybridoma (2005) 06/2012; 31(3):176-9. · 0.33 Impact Factor
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    ABSTRACT: A Gram-negative, rod-shaped, non-spore forming and non-motile bacterium, designated strain NUM 1720(T) , was isolated from the oral cavity of bears. Based on 16S rRNA gene sequence similarity, strain NUM 1720(T) was shown to be related to Gibbsiella quercinecans (99.4%). The gyrB and rpoB gene sequences of strain NUM 1720T showed 98.0% and 98.2% similarity with those of G. quercinecans. The DNA-DNA hybridization value of strain NUM 1720(T) with G. quercinecans was 63.8%. The G + C content of the genomic DNA of the isolates was 55.0 mol%. Fatty acid analysis data supported the affiliation of strain NUM 1720(T) to the genus Gibbsiella. The major menaquinone and ubiquinone were MK-8 and Q-8, respectively. Strain NUM 1720(T) can be differed from G. quercinecans by the reactions to acetoin, inositol and D-arabinose. Strain NUM 1720(T) therefore represents a novel species, for which the name Gibbsiella dentisursi sp. nov. is proposed, with type strain NUM 1720(T) (= JCM 17201(T) = DSM 23818(T)).
    Microbiology and Immunology 04/2012; 56(8):506-12. · 1.55 Impact Factor
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    ABSTRACT: Oral immunotherapy is potentially useful for the treatment of allergic diseases. We previously demonstrated that allergen-induced airway inflammation and immunoglobulin E (IgE) production in mice were suppressed by oral administration of high-dose transgenic (Tg) rice seeds (approximately 50 g/kg/day) expressing a T cell epitope of Dermatophagoides pteronyssinus group 1 allergen (Der p 1). However, this amount of Tg rice seeds was not realistic in our daily life. In this study, allergen-induced airway inflammation and IgE production following oral immunotherapy with a realistic (lowest) dose of Tg rice seeds were investigated. Mice orally administered with Tg or non-Tg rice seeds at approximately 5 g/kg/day for 1 week were immunized with recombinant Der p 1, and then challenged with the corresponding allergen. The infiltration of inflammatory cells into the airways and the levels of allergen-specific serum IgE were examined. Low-dose oral administration of Tg rice seeds significantly inhibited the allergen-induced infiltration of eosinophils and lymphocytes into the airways, but allergen-specific IgE synthesis was not changed. Low-dose oral immunotherapy with Tg rice seeds could suppress allergen-induced airway inflammation through mechanisms other than the downregulation of IgE synthesis.
    International Archives of Allergy and Immunology 01/2012; 158 Suppl 1:66-9. · 2.25 Impact Factor
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    ABSTRACT: Celiac disease (CD) is a chronic immune-mediated intestinal inflammatory disorder afflicting genetically susceptible individuals triggered by the consumption of dietary cereals with high gluten content. As with many other organ-specific autoimmune diseases, the dominant tissue-destructive inflammation in CD is T cell-mediated. The proinflammatory cytokine IL-15 that is overexpressed in the intestinal epithelium of CD patients has emerged as a pivotal element that orchestrates intestinal inflammation and T cell-mediated autoimmune tissue destruction. Although no animal model exists that recapitulates the full spectrum of CD pathophysiology, we have previously reported that transgenic mice that overexpress human IL-15 in enterocytes (T3(b)-hlL-15 Tg) display many of the T cell-mediated pathologic features seen in CD. Extending these observations, we now report that T3(b)-hlL-15 Tg mice in addition to recapitulating T cell-mediated effects also display autoantibodies including those against tissue transglutaminase 2 and extensive lamina propria plasmacytosis, all of which are characteristic of CD, thereby reflecting the possibility that locally expressed IL-15 drives both T and B cell pathologic effects seen in CD. More importantly, these findings support the validity and utility of T3(b)-hlL-15 Tg mice as a reasonable model to investigate not only tissue-destructive pathologic processes in CD, but also to explore novel therapeutic modalities for the treatment of this disease.
    Journal of Clinical Immunology 09/2011; 31(6):1038-44. · 3.38 Impact Factor
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    ABSTRACT: This study tested the feasibility of oral immunotherapy for bronchial asthma using a newly developed subunit vaccine in which a fragment (p45-145) of mite allergen (Der p 1) containing immunodominant human and mouse T cell epitopes was encapsulated in endoplasmic reticulum-derived protein bodies of transgenic (Tg) rice seed. Allergen-specific serum immunoglobulin responses, T cell proliferation, Th1/Th2 cytokine production, airway inflammatory cell infiltration, bronchial hyper-responsiveness (BHR) and lung histology were investigated in allergen-immunized and -challenged mice. Prophylactic oral vaccination with the Tg rice seeds clearly reduced the serum levels of allergen-specific IgE and IgG. Allergen-induced CD4(+) T cell proliferation and production of Th2 cytokines in vitro, infiltration of eosinophils, neutrophils and mononuclear cells into the airways and BHR were also inhibited by oral vaccination. The effects of the vaccine were antigen-specific immune response because the levels of specific IgE and IgG in mice immunized with Der f 2 or ovalbumin were not significantly suppressed by oral vaccination with the Der p 1 expressing Tg rice. Thus, the vaccine does not induce nonspecific bystander suppression, which has been a problem with many oral tolerance regimens. These results suggest that our novel vaccine strategy is a promising approach for allergen-specific oral immunotherapy against allergic diseases including bronchial asthma.
    Plant Biotechnology Journal 03/2011; 9(9):982-90. · 6.28 Impact Factor
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    ABSTRACT: Streptococcus mutans and Streptococcus sobrinus are considered to be important bacterial species in the initiation of human dental caries. Therefore, the establishment of a reliable genotyping method to distinguish S. mutans from S. sobrinus is of central importance. We assessed the usefulness of repetitive extragenic palindromic polymerase chain reaction (rep-PCR) using ERIC primer banding patterns in differentiating S. mutans and S. sobrinus. Five S. mutans and two S. sobrinus prototype strains and 50 clinical isolates (38 S. mutans serotype c, 4 S. sobrinus serotype d, and 8 S. sobrinus serotype g) were examined. The banding patterns of amplicons generated were compared among the prototype strains and clinical isolates, to find common bands that distinguish S. mutans and S. sobrinus. Multiple banding patterns were seen with all strains tested. The representative strains of S. mutans tested revealed six unique, strong bands at 2,000 bp, 1,700 bp, 1,400 bp, 1,100 bp, 850 bp, and 250 bp, whereas S. sobrinus had seven strong bands at 2,000 bp, 1,800 bp, 1,100 bp, 900 bp, 800 bp, 600 bp, and 550 bp. The band at 1,100 bp was the only band that was observed in both S. mutans and S. sobrinus. Furthermore, most clinical S. mutans isolates revealed identical banding patterns. All S. mutans had amplicons at 1,700 bp, 850 bp, and 250 bp, whereas those of S. sobrinus were at 1,100 bp, 900 bp, and 800 bp. These results indicate that using rep-PCR with the ERIC primers can distinguish between S. mutans and S. sobrinus.
    Journal of Oral Microbiology 01/2011; 3.
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    ABSTRACT: Aggregatibacter actinomycetemcomitans is usually isolated from the oral cavity where it is associated with active periodontitis. The species can be divided into six serotypes (a-f) according to their surface carbohydrate antigens. However, some clinical isolates cannot be grouped within these six serotypes. Gram-negative, facultative anaerobic, catalase-positive coccobacilli were isolated from a patient with periodontitis and identified by employing genetic, biochemical and serological analyses. Phenotypic data identified the isolate as A. actinomycetemcomitans. Serotype-specific polysaccharide antigen from the isolate was untypeable by immunodiffusion testing in comparison with reference A. actinomycetemcomitans serotype a to f strains. Biofilm formation by the isolate was strong but cytotoxic activity was low. Gas chromatography/mass spectroscopy analysis of partially methylated alditol acetates from surface polysaccharide showed the presence of 2,4-di-O-methyl-rhamnose and 2,3,6-tri-O-methyl-glucose, with a 1 : 1 m ratio. The (1)H- and (13)C-nuclear magnetic resonance spectra of the antigen showed that both constituent glycoses had alpha-anomeric configuration. It is proposed that the untyped strain is a new A. actinomycetemcomitans serotype, designated serotype g.
    Molecular oral microbiology. 06/2010; 25(3):200-6.
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    ABSTRACT: Three Gram-positive, catalase-negative, coccus-shaped organisms were isolated from the oral cavities of bears. The isolates were tentatively identified as a streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequencing studies confirmed that the organisms were members of the genus Streptococcus, but they did not correspond to any recognized species of the genus. The nearest phylogenetic relative of the new isolates was Streptococcus ratti ATCC 19645(T) (98.6 %), however, DNA-DNA hybridization analysis showed that the isolates displayed less than 15 % DNA-DNA relatedness with the type strain of S. ratti. Colonies of the novel strains grown on mitis salivarius agar showed an extracellular polysaccharide-producing colony morphology. Based on phenotypic and phylogenetic evidence, it is proposed that the novel isolates are classified in the genus Streptococcus as Streptococcus ursoris sp. nov. The type strain of S. ursoris is NUM 1615(T) (=JCM 16316(T)=DSM 22768(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2010; 61(Pt 1):40-4. · 2.11 Impact Factor
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    ABSTRACT: Four strains (NUM 1903(T), NUM 1904, NUM 1912 and NUM 1925) that were obligately anaerobic, pigmented, Gram-negative-staining rods were isolated from the oral cavity of donkeys. These strains were analysed using the Rapid ID 32A, API 20A and API ZYM systems, by DNA-DNA hybridization with other related species and by 16S rRNA gene sequencing. 16S rRNA gene sequence analysis showed that each of the new isolates was a member of the genus Prevotella and related to Prevotella multiformis PPPA21(T), showing about 93 % sequence similarity. Based on phylogenetic and phenotypic evidence, it is proposed that the four strains are representatives of a novel species, for which the name Prevotella dentasini sp. nov. is proposed. The type strain is NUM 1903(T) (=JCM 15908(T)=DSM 22229(T)).
    International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 7):1637-9. · 2.11 Impact Factor
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    ABSTRACT: Four Gram-stain-positive, catalase-negative, coccoid-shaped isolates were obtained from the oral cavities of wild boars and characterized by phenotypic and phylogenetic studies. On the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed that the organisms are members of the genus Streptococcus, with Streptococcus equi subsp. equi ATCC 33398(T) as their closest phylogenetic relative (94.7 % similarity). DNA-DNA hybridization analysis showed that the isolates displayed less than 10 % relatedness to Streptococcus equi subsp. equi DSM 20561(T). From the phylogenetic and phenotypic evidence, the four isolates represent a novel species of the genus Streptococcus, for which the name Streptococcus dentapri sp. nov. (type strain NUM 1529(T) =JCM 15752(T) =DSM 21999(T)) is proposed.
    International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 4):820-3. · 2.11 Impact Factor
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    M. Hirasawa, K. Takada, T. Ikeda
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    ABSTRACT: The mechanism for adherence of Streptococcus mitis to human buccal epithelial cells was studied in vitro. The optimal conditions for such adherence were incubation for 60 min at 37d`C, pH of 7-8, and a ratio of bacterial to epithelial cells of 200:1. Eight bacterial cells were attached per epithelial cell under these conditions. Saliva-coating S. mitis increased adhesion by approximately four-fold. A reduction of the adherence was observed when bacterial cells were coated with heat- or trypsin-treated saliva. The adherence of saliva-coated S. mitis was almost completely inhibited by N-acetylneuraminic acid, and to some extent by glucose and galactose. Treatment of saliva-coated bacterial cells by divalent cations or a chaotropic anion had no effect on adherence to these cells. Thus, it would appear that glucose, galactose and especially N-acetylneuraminic acid sensitive lectins are important in the adherence mechanism of S. mitis.
    07/2009; 7(3):153-159.
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    ABSTRACT: Streptococcus sobrinus exhibits more significant dextran-dependent aggregation mediated by glucan-binding proteins than Streptococcus mutans. We have identified four glucan-binding protein C gene (gbpC) homologues designated as gbpC1, gbpC2, dblA and dblB in S. sobrinus in contrast to the single gene gbpC in S. mutans. We attempted to determine which gene is most responsible for the dextran-dependent aggregation of S. sobrinus. We introduced mutation with a chemical mutagen, 1-methyl-3-nitro-1-nitrosoguanidine, into S. sobrinus strain 6715 and analysed the four gbpC homologous gene sequences in the parental strain 6715 and an obtained aggregation-negative mutant NUM-Ssg99. We also examined the localization of proteins encoded by these genes in the mutant NUM-Ssg99. The nucleotide sequences of the gbpC1, gbpC2 and dblA genes in NUM-Ssg99 were 100% identical to the homologous genes in parental strain 6715. In contrast, a truncated mutation was detected in the dblB gene and the mutant protein devoid of the LPXTG motif was confirmed by Western blot analysis to be released into the extracellular milieu. We conclude that the dblB gene among the four GbpC homologous protein genes is most responsible for aggregation in strain 6715.
    Oral Microbiology and Immunology 07/2009; 24(3):224-30. · 2.81 Impact Factor
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    ABSTRACT: Conventional selective media have been used for the selection of Aggregatibacter (Actinobacillus) actinomycetemcomitans in clinical samples. The proportion of A. actinomycetemcomitans grown on the selective media in vitro may not reflect the true counts in vivo because of the low selectivity. A novel selective medium, designated AASM, was developed for the isolation of A. actinomycetemcomitans. AASM was prepared by adding of 200 microg/mL of vancomycin and 10 U/mL of bacitracin to AAGM, which contains dextrose, sodium bicarbonate, trypticase soy, yeast extract and agar. Clinical efficacy was evaluated by the recovery, on AASM, of A. actinomycetemcomitans from subgingival samples of 44 periodontally healthy subjects and 76 patients with chronic periodontitis. All serotypes (a-f) of A. actinomycetemcomitans strains grew well, and the average growth recovery of A. actinomycetemcomitans on AASM medium was 94.4% (80.0-109.7%) of that on AAGM. The exclusive rate of other bacteria was 99.9% in clinical samples cultured on AASM. A. actinomycetemcomitans was not detected in periodontally healthy persons but was detected in 25 (32.9%) patients with chronic periodontitis. The predominant serotype was c, detected in 11 subjects. The new selective medium, AASM, was highly selective for A. actinomycetemcomitans, eliminated possible false-positive results and was useful for the isolation of A. actinomycetemcomitans from clinical samples.
    Journal of Periodontal Research 06/2008; 43(5):544-8. · 1.99 Impact Factor