-
[show abstract]
[hide abstract]
ABSTRACT: To compare the effects of 25 kDa full-length rhAm and porcine EMPs on cell behaviors of human periodontal ligament fibroblasts (HPDLF) and foreskin fibroblasts(HFF).
rhAm was induced by BL21/pET28a-His-SUMO-rhAm express system, and 25 kDa full-length rhAm was analyzed by SDS-PAGE and Western blot. EMPs were extracted by acetic acid method. HPDLF and HFF were cultured in vitro. The cells were treated with rhAm and EMPs at different concentrations. The cell adhesion, proliferation and migration assays were qualitatively analyzed. The data was statistically analyzed with SAS 5.0 software package.
10-20 μg/mL rhAm significantly promoted the adhesion, proliferation and migration of HPDLF and HFF (P<0.05), but no significant difference between two proteins was found (P>0.05).
25 kDa rhAm and EMPs shows similar biological effects on fibroblast, which indicates that rhAm may play an important role in the periodontal regeneration through the activation of fibroblasts. Supported by National Natural Science Foundation of China (81070838, 81271156) and Biomedical Engineering Cross Research Foundation of Shanghai Jiao Tong University (YG2011MS31).
Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2013; 22(1):7-12.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the virulent properties of P. gingivalis clinical strains.
By using mouse abscess model (MAM),six P. gingivalis clinical strains, L2, L3, L4, L5, L11 and L12 were subcutaneously inoculated into the central back of BALB/C mice. The clinical signs of the mice were observed and the virulent properties of the clinical strains were analyzed by comparison with type strains of W83 and ATCC 33277. SPSS11.5 software package was used for statistical analysis.
According to the criteria established in previous reports, L3, ATCC33277, and W83 produced a localized abscess at the site of injection and were categorized as noninvasive. L2, L5, L11, and L12-induced lesions spread to distant organs and may produce severe systemic reactions and these strains were classified as invasive.
Clinical strains from Chinese patients showed similarities to type strains in MAM. The virulent properties of P. gingivalis clinical strains are quite different from each other. Supported by National Natural Science Foundation of China (30901672),Shanghai Leading Academic Discipline Project (S30206-fzd03) and Innovative Research Project of Shanghai Municipal Education Commission(09YZ78).
Shanghai kou qiang yi xue = Shanghai journal of stomatology 06/2012; 21(3):241-5.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the potential effect of recombinant 25kDa porcine amelogenin (rPAm) on attachment, proliferation and migration of primarily cultured human gingival epithelial cells (HGEC).
The second passage of HGECs were exposed to different concentrations of rPAm (0, 5, 10, 20μg/mL, respectively). Proliferation and attachment activities was measured by using cell counting method. Cellular migration was assayed by using an in vitro wound healing model. The data was quantified by the analysis of GraphPad Prism software.
rPAm inhibited HGEC attachment in the adhesion assay, the effect was depended on time and rPAm dose. rPAm suppressed the growth rate of HGEC, that was also dose and time dependent. rPAm inhibited the migration ability of HGEC, the concentration of 20μg/mL group had the most significant effect.
rPAm significantly inhibit the growth rate, cell adhesion and migration of HGEC, and the effect was dose- and time- dependent. Supported by National Natural Science Foundation of China(30672315) and Research Fund of Science and Technology Commission of Shanghai Municipality(08DZ2271100).
Shanghai kou qiang yi xue = Shanghai journal of stomatology 06/2012; 21(3):257-61.
-
[show abstract]
[hide abstract]
ABSTRACT: Periodontal ligament (PDL) cells are fibroblasts that play key roles in tissue integrity, periodontal inflammation and tissue regeneration in the periodontium. The periodontal tissue destruction in periodontitis is mediated by host tissue-produced inflammatory cytokines, including interleukin-1β (IL-1β). Here, we report the expression of G protein-coupled receptor 30 (GPR30, also known as G protein-coupled estrogen receptor 1 GPER) in human PDL cells and its regulation by IL-1β. IL-1β-induced GPR30 expression in human PDL cells leads to the activation of multiple signaling pathways, including MAPK, NF-κB and PI3K. In contrast, genistein, an estrogen receptor ligand, postpones the activation of MAPKs induced by IL-1β. Moreover, the inhibition of GPR30 by G15, a GPR30-specific antagonist, eliminates this delay. Thus, genistein plays a role in the regulation of MAPK activation via GPR30, and GPR30 represents a novel target regulated by steroid hormones in PDL cells.
Archives of Biochemistry and Biophysics 04/2012; 522(1):9-16. · 2.93 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To analyze bacteria community structure changes in subgingival plaque after initial therapy, and to provide experimental evidences for clinical decision.
Six patients with chronic periodontitis were chosen. Subgingival plaque samples, as well as clinical indexes, were collected at baseline and six weeks after initial therapy. The generated two different 16S rDNA fragments of subgingival plaque samples were separated by denaturing gel, creating bands patterns representative of community structure. Subsequent cluster analysis was made.
The clinical indexes were improved significantly. The diversity of population of clinical subgingival samples was not significantly different between baseline and 6 weeks after initial therapy. Through cluster analysis, it was confirmed that same patient got similar subgingival plaque between baseline and 6 weeks after treatment.
Same patient tend to get similar subgingival plaque between baseline and after initial therapy. DGGE can detect the microbial composition of subgingival plaque.
Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2012; 21(1):73-8.
-
[show abstract]
[hide abstract]
ABSTRACT: Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS) has been reported to induce the expression of vascular cell adhesion molecule-1 (VCAM-1) in vascular endothelial cells. This finding suggests the potential roles for Pg in the pathogenesis of atherosclerosis. However, the mechanism involved in Pg LPS-induced VCAM-1 production in endothelial cells remains unclear.
Quantitative real-time polymerase chain reaction and Western blotting were used, respectively, to investigate the mRNA expression and protein production of VCAM-1 in human aortic endothelial cells (HAECs) induced by Pg LPS. The involvement of the p38 mitogen-activated protein kinase (p38 MAPK) cell signaling pathway in VCAM-1 expression was investigated by assays with specific inhibitors.
Pg LPS-induced expression in HAECs of VCAM-1 occurred in a dose- and time-dependent manner. In addition, the p38 MAPK inhibitor (SB 203580) significantly attenuated Pg LPS-induced VCAM-1 expression.
Activation of p38 MAPK is at least partially involved in Pg LPS-induced VCAM-1 expression in HAECs, which may contribute to the acceleration of atherosclerosis.
Journal of Periodontology 11/2011; 83(7):955-62. · 2.60 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To compare the proteomic profiles of whole unstimulated saliva (WUS) of subjects with chronic periodontitis (CP) with that of healthy volunteers, and to identify proteins.
WUS was obtained from five subjects with CP and five healthy subjects, and proteins were separated using two-dimensional gel electrophoresis (2-DE). Proteins, the level of which was significantly different among the two groups, were identified by computer image analyses and subsequent matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS).
Seventeen differentially expressed points in the 2-DE gel between the two samples were found by professional software. And protein types of thirteen points were then identified. Among them, the point 737, 725, 692, 986 was the same protein, as well as the point 716, 535. They were respectively serum albumin; beta-fibrin; chain B, human serum transferrin, recombinant N-terminal lobe, Apo form, crystal form 2; Ig alpha-1 chain C region; the GA module complexed with human serum albumin (HAB-GA); chain B, crystal structure of a Rca-Rhogdi complexes; PRO2044; hypothetical protein; unnamed protein. Compared with whole saliva of healthy control subjects, the levels of them were increased in whole unstimulated saliva of CP subjects.
Comparison of the proteomic profiles of WUS of CP subjects with that of healthy control subjects reveals at least 9 differential proteins. The approach might be helpful to better understand to the etiology of CP.
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 10/2011; 29(5):519-21, 525.
-
[show abstract]
[hide abstract]
ABSTRACT: MicroRNAs (miRNAs) have been demonstrated to play an important role in regulation of the immunoinflammatory response; however, the function of miRNAs in periodontal inflammation has not been investigated. The objective of this study was to explore the properties of miRNAs in periodontal inflammation by comparing miRNA profiles of inflamed and healthy gingival tissues. Gingival tissues were obtained from 10 periodontitis patients and 10 healthy subjects. After RNA extraction, miRNA profiles were analyzed by microarray, and expression levels of selected miRNAs were confirmed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Analyses using two computational methods, Targetscan and MicroRNA.org, were combined to identify common targets of these miRNAs. Finally, the individual miRNA expression levels of three toll-like receptor (TLR)-related miRNAs from inflamed and healthy gingival tissues were evaluated by RT-PCR. Ninety-one miRNAs were found to be upregulated and thirty-four downregulated over two-fold in inflamed gingival tissue compared with those in healthy gingival tissue. Twelve selected inflammatory-related miRNAs, hsa-miR-126*, hsa-miR-20a, hsa-miR-142-3p, hsa-miR-19a, hsa-let-7f, hsa-miR-203, hsa-miR-17, hsa-miR-223, hsa-miR-146b, hsa-miR-146a, hsa-miR-155, and hsa-miR-205 showed comparable expression levels by microarray and real-time quantitative RT-PCR analyses. In addition, the putative inflammation targets of these miRNAs were predicted, and three that were tested (hsa-miRNA-146a, hsa-miRNA-146b, and hsa-miRNA-155), showed significant differences between inflamed and healthy gingiva. This remarkable difference in miRNA profiles between periodontal diseased and healthy gingiva implicates a probable close relationship between miRNAs and periodontal inflammation. The data also suggest that the regulation of TLRs in periodontal inflammation may involve miRNA pathways.
International Journal of Oral Science 07/2011; 3(3):125-34. · 1.41 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The purpose of this study was to evaluate the clinical effects of photodynamic therapy (PDT) for scaling and root planing (SRP) in the treatment of chronic periodontitis.
PDT has become a potential treatment of infectious diseases with the development of laser medicine. However, there are very limited data from clinical trials to evaluate the effect of PDT in the treatment of periodontitis.
Fifty-eight patients with chronic periodontitis were included and divided into three groups. They were treated with SRP alone, SRP followed by one PDT, or SRP followed by two PDT treatments. PDT was performed at sites with a probing depth (PD) ≥5 mm by using Periowave(TM) therapy. Periodontal values of bleeding on probing (BOP), PD, and clinic attachment loss (CAL) were examined at baseline, 6 wk after treatment, and 12 wk after treatment.
Compared with the baseline, sites with baseline PD ≥5 mm in all three groups showed significant reductions of PD, CAL, and BOP at 6 and 12 wk after treatment. Although there were no differences between the three groups for PD and CAL in all three examinations, the presence of BOP sites at 12 wk, but not 6 wk, after SRP treatment significantly decreased in groups with PDT in comparison with SRP alone.
PDT may serve as an adjunctive therapy to SRP treatment in periodontal pockets with PD ≥5 mm to reduce the presence of bleeding in these lesions.
Photomedicine and laser surgery 01/2011; 29(1):33-7. · 1.76 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: As an active remote sensor technology, the terrestrial laser scanner is widely used for direct generation of a three-dimensional (3D) image of an object in the fields of geodesy, surveying, and photogrammetry. In this article, a new laser scanner using array avalanche photodiodes, as designed by the Shanghai Institute of Technical Physics of the Chinese Academy of Sciences, is introduced for rapid collection of 3D data. The system structure of the new laser scanner is first presented, and a mathematical model is further derived to transform the original data to the 3D coordinates of the object in a user-defined coordinate system. The performance of the new laser scanner is tested through a comprehensive experiment. The result shows that the new laser scanner can scan a scene with a field view of 30° × 30° in 0.2 s and that, with respect to the point clouds obtained on the wall and ground floor surfaces, the root mean square errors for fitting the two planes are 0.21 and 0.01 cm, respectively. The primary advantages of the developed laser scanner include: (i) with a line scanning mode, the new scanner achieves simultaneously the 3D coordinates of 24 points per single laser pulse, which enables it to scan faster than traditional scanners with a point scanning mode and (ii) the new scanner makes use of two galvanometric mirrors to deflect the laser beam in both the horizontal and the vertical directions. This capability makes the instrument smaller and lighter, which is more acceptable for users.
Applied Optics 12/2010; 49(34):H11-9. · 1.41 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: DGGE of 16S rDNA is one of the most frequently used methods to study microbial communities. In this study, the DGGE profiles of different 16S rDNA regions of the periodontal pathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella nigrescens were investigated. The results suggested that V3-V5 and V6-V8 fragments may be suitable for community analysis of subgingival bacteria. Further analysis of subgingival samples with V3-V5 and V6-V8 regions as target fragments suggested that, in chronic periodontitis, re-colonization by periodontal bacteria with a population very similar to the baseline may occur by 6 weeks after mechanical debridement.
Microbiology and Immunology 11/2010; 54(11):702-6. · 1.30 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate the therapeutic effects of doxycycline nano-liposome slow-release gel on the periodontitis in an established rat model.
The biocompatibility was tested by oral perfusion sample gel for long term observation. Doxycycline nano-gel was used to treat the established rat model of periodontitis. The rats were selected randomly and divided into five groups: blank group, doxycycline nano-gel treated group, minocycline-treated group, matrix group and periodontitis model group. MMP-8 and TIMP-1 in the sulcus fluid were detected by real-time PCR. The data was analysed using SAS13.3 software package for Student's t test.
Doxycycline nano-gel had excellent biocompatibility from weight measure and tissue section evaluation. The results in periodontitis rats demonstrated that doxycycline nano-gel could ameliorate periodontitis condition dramatically compared with the control group, the restoration even better than minocycline-treated group.
Doxycycline nano-liposome slow-release gel improves rat periodontitis by decreasing MMP-8 level, which provides valuable evidence to promote doxycycline nano-gel clinical application.
Shanghai kou qiang yi xue = Shanghai journal of stomatology 10/2010; 19(5):508-11.
-
[show abstract]
[hide abstract]
ABSTRACT: Periodontitis is a bacterially induced chronic inflammatory disease. Toll-like receptors (TLRs), which could recognize microbial pathogens, are important components in the innate and adaptive immune systems. Both qualitatively and quantitatively distinct immune responses might result from different bacteria stimulation and the triggering of different TLRs. This study explores the interaction of Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) with TLR2 and TLR4.
We studied the gene expression changes of TLR2 and TLR4 and cytokine production (interleukin-1β, -6, -8, -10, and tumor necrosis factor-alpha) in human periodontal ligament cells (HPDLCs) stimulated with heat-killed bacteria or P. gingivalis lipopolysaccharide (LPS) in the presence or absence of monoclonal antibodies to TLR2 or TLR4 (anti-TLR2/4 mAb).
Both test bacteria and 10 microg/ml P. gingivalis LPS treatment increased the gene expression of TLR2 and TLR4 and cytokine production in HPDLCs. In addition, these upregulations could be blocked by anti-TLR2/4 mAb. However, the expression of TLR4 mRNA in HPDLCs stimulated with 1 microg/ml P. gingivalis LPS was not increased. No differences were found in the cytokine production caused by 1 microg/ml P. gingivalis LPS treatment in the presence or absence of anti-TLR4 mAb.
These patterns of gene expression and cytokine production indicate that Gram-negative periodontal bacteria or their LPS might play a role in triggering TLR2 and/or TLR4, and be of importance for the immune responses in periodontitis.
Journal of Periodontology 10/2010; 81(10):1488-96. · 2.60 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate the effect of periodontal initial therapy on clinical parameters and subgingival periodontal pathogen in patients with chronic periodontitis.
One hundred and twenty patients with chronic periodontitis were included. Probing depth (PD), attachment loss (AL), plaque index (PLI) and gingival index (GI) were evaluated at baseline and after-initial therapy. P.g and A.a in subgingival plaque were investigated by real-time PCR. Data was statistically analyzed by SAS6.12 software for Student's t test.
The PD, AL, PLI and GI were significantly decreased after periodontal initial therapy (P<0.01), and meanwhile the ratio of P.g versus total bacteria was significantly decreased after-initial therapy (P<0.05). However, the change of ratio of A.a versus total bacteria was not significant (P>0.05).
Periodontal initial therapy could effectively control the inflammation of chronic periodontitis, and decrease the ratio of P.g in subgingival plaque.
Shanghai kou qiang yi xue = Shanghai journal of stomatology 06/2010; 19(3):225-7.
-
[show abstract]
[hide abstract]
ABSTRACT: Previous studies have assumed that amelogenin is responsible for the therapeutic effect of the enamel matrix derivative (EMD) in periodontal tissue healing and regeneration. However, it is difficult to confirm this hypothesis because both the EMD and the amelogenins are complex mixtures of multiple proteins. Further adding to the difficulties is the fact that periodontal tissue regeneration involves various types of cells and a sequence of associated cellular events including the attachment, migration and proliferation of various cells. In this study, we investigated the potential effect of a 25-kDa recombinant porcine amelogenin (rPAm) on primarily cultured periodontal ligament fibroblasts (PDLF), gingival fibroblasts (GF) and gingival epithelial cells (GEC). The cells were treated with 25-kDa recombinant porcine amelogenin at a concentration of 10 microg/mL. We found that rPAm significantly promoted the proliferation and migration of PDLF, but not their adhesion. Similarly, the proliferation and adhesion of GF were significantly enhanced by treatment with rPAm, while migration was greatly inhibited. Interestingly, this recombinant protein inhibited the growth rate, cell adhesion and migration of GEC. These data suggest that rPAm may play an essential role in periodontal regeneration through the activation of periodontal fibroblasts and inhibition of the cellular behaviors of gingival epithelial cells.
Biochemical and Biophysical Research Communications 03/2010; 394(3):581-6. · 2.48 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the effect of nifedipine(NIF) on transcription of bcl-2 in human gingival epithelial cells(HGECs) in vitro and to study the pathogenesis of epithelial thickening in drug-induced gingival overgrowth(DGO).
The gingival tissues obtained from periodontal surgeries were digested with enzyme and HGECs were cultured in vitro; HGECs were identified by immunohistochemistry; bcl-2 mRNA levels were quantitated by Real-time PCR 24 hours and 48 hours after cells stimulated by NIF with different concentration (0microg/ml, 1microg/ml,2microg/ml,3microg/ml), in which 0microg/ml NIF as blank control. The data was analyzed by one-way ANOVA using SPSS 11.0 software package.
HGECs cultured in vitro showed keratin positive signal and vimentin negative signal; the level of bcl-2 mRNA increased with NIF 3microg/ml after 24 hours treatment, which appeared significant increase compared with blank control (P<0.05); after 48 hours treatment the level of bcl-2 mRNA in the groups of 2microg/ml and 3microg/ml showed significant increase compared with blank control (P<0.05).
NIF regulates the level of bcl-2 mRNA.
Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2010; 19(1):81-5.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the effect of hypoxia on proliferation and expression of HIF-1alpha and Caspase-3 in human periodontal ligament cells (PDLCs).
Human PDLCs were exposed to cobalt chloride in order to mimic hypoxia. Cell viability of PDLCs was determined by MTT methods. Expression of HIF-1alpha and Caspase-3 was measured by real time PCR and Western blot. The data was statistically analyzed with SAS6.12 software package for one-way ANOVA.
Cell viability of PDLCs significantly decreased when exposed to hypoxia in a time- and dose-dependent manner. Hypoxia induced the expression of HIF-1alpha,up-regulated the expression of Caspase-3.
Hypoxia inhibits cell proliferation, which involves the expression of HIF-1alpha and Caspase-3, resulting in the production of the apoptosis. The results suggest that hypoxia may play a role in the induction and progression of chronic periodontitis. Supported by National Natural Science Foundation of China(Grant No.30801292), Shanghai Leading Academic Discipline (Grant No.S30206) and Research Fund for Excellent Young Teachers of Shanghai Municipal College and University (Grant No.JDY07059).
Shanghai kou qiang yi xue = Shanghai journal of stomatology 10/2009; 18(5):489-92.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate gene expression of amelogenin (Am) in human gingival epithelial cells(HGEC) and also other oral ectomesenchyme cells (human gingival fibroblasts, human periodontal ligament fibroblasts and human pulp cells).
The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.
There was no amelogenin expression detected in any of the cells.
These findings suggest that amelogenin expression could not be detected in cultured human periodontium-related cells. Supported by National Natural Science Foundation of China (Grant No.30672315) and Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).
Shanghai kou qiang yi xue = Shanghai journal of stomatology 10/2009; 18(5):499-504.
-
[show abstract]
[hide abstract]
ABSTRACT: Image segmentation is an important step for classification and feature extraction of high resolution remote sensing image. The purpose of this study is to find an improved segmentation method suitable for high resolution remote sensing image. Firstly a region homogeneity indictor called H index was introduced. Then the optimized edge gradient was obtained based on the integration of Canny operator and H index. A watershed transformation followed up to acquire the initial segmentation of the remote sensing image. To eliminate the over-segmentation, a multi-scale merging according to object-oriented principle was finally conducted. A multi-spectrum QuickBird remote sensing image was segmented per the above-mentioned method. The improved H gradient image effectively overcame the limitations of week edges in high resolution remote sensing image, and on the whole the QuickBird image was segmented into homogeneity objects. It proves that the improved segmentation method is suitable to high resolution remote sensing images.
Urban Remote Sensing Event, 2009 Joint; 06/2009
-
[show abstract]
[hide abstract]
ABSTRACT: In contemporary dental care, an increasing number of adult patients with periodontal disease are seeking orthodontic treatment. Achieving optimal results in such adult patients is difficult because decreased posterior tooth anchorage is risky. This case report demonstrates the use of miniscrew implant anchorage (MIA) in a Chinese male 21 years 5 months of age with maxillary and mandibular anterior dental spacing, bimaxillary protrusion, and severe bone loss caused by periodontal disease. Prior to orthodontic treatment, the patient underwent treatment to control his periodontitis. The patient was treated with 0.022-in straight-wire orthodontic appliances. After 17 months of active orthodontic treatment, the patient had healthier periodontal tissue with increased bone support, as well as improved facial esthetics and a functional occlusion. The results demonstrate that MIA is useful in enhancing anchorage in patients with bone loss associated with severe periodontal disease.
World journal of orthodontics 02/2009; 10(1):49-56.
