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ABSTRACT: In the recent years several studies showed the association between body iron stores, represented by serum ferritin, and atherosclerosis. It was proposed that iron bound to ferritin catalyzes the formation of highly reactive forms of oxygen free radicals which subsequently cause the oxidative modification of atherogenic lipoproteins. Aim of our study was to compare serum ferritin concentrations and certain markers of oxidative stress in patients with and without coronarographically assessed coronary vascular disease.
Measurements were performed in 216 subjects at the age of 35-60 years. The patient group included 76 patients with coronarographically assessed coronary vascular disease (CVD) (mean age 51.16 +/- 5.713 years) and 140 healthy controls (mean age 50.21 +/- 5.331 years). The plasma concentration of ferritin was higher in patients (169.04 +/- 63.899 micrograms/l) than controls (87.70 +/- 41.394 micrograms/l), p < 0.001. The group of patients revealed significantly lower plasma concentrations of anti-oxLDL antibodies, nitrites/nitrates, tocopherol and high density lipoprotein cholesterol (HDL-cholesterol) than controls; on the contrary patients had significantly higher concentrations of hemoglobin, thrombocytes and triacylglycerols. In the whole cohort of investigated subjects, ferritin correlated positively with retinol, body mass index (BMI), total-cholesterol, triacylglycerols, low density lipoprotein cholesterol (LDL-cholesterol), blood glucose, creatinine, uric acid, alaninaminotransferase (ALT), aspartateaminotransferase (AST), hematocrite, erythrocytes, with occurrence of CVD and with sex. Inverse correlation was observed between ferritin and HDL-cholesterol.
Our observations are consistent with the hypothesis that high stored iron levels, measured by serum ferritin concentrations, may contribute to the oxidative stress and thus elevate the risk for development of CVD.
Vnitr̆ní lékar̆ství 04/2004; 50(3):197-202.
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ABSTRACT: Atherogenic lipoproteins can cause endothelial dysfunction in the initial stage of atherogenesis. In our study we examined 134 patients with defined hyperlipoproteinemia (non-HDL cholesterol>4.1 mmol/l or triglycerides>2.5 mmol/l or taking any of lipid lowering drugs)--94 men and 40 women. The subgroup of controls of comparable age contained 54 normolipidemic individuals--30 men and 24 women. Patients with hyperlipoproteinemia revealed significantly lower ability of endothelium-dependent flow-mediated vasodilation (EDV) measured on brachial artery (4.13+/-3.07 vs. 5.41+/-3.82 %; p=0.032) and higher carotid intima media thickness than normolipidemic controls (0.68+/-0.22 vs. 0.58+/-0.15 mm; p=0.005). In regression analysis, EDV correlated significantly with plasma concentrations of oxLDL (p<0.05) HDL-cholesterol (p<0.05), Apo A1 (p<0.05), ATI (p<0.01) and non-HDL cholesterol (p<0.05). Patients with hyperlipoproteinemia showed higher plasma levels of oxLDL (65.77+/-9.54 vs. 56.49+/-7.80 U/l; p=0.015), malondialdehyde (0.89+/-0.09 vs. 0.73+/-0.08 micromol/l; p=0.010) and nitrites/nitrates (20.42+/-4.88 vs. 16.37+/-4.44 micromol/l; p=0.018) indicating possible higher long-term oxidative stress in these patients.
Physiological research / Academia Scientiarum Bohemoslovaca 02/2004; 53(5):471-80. · 1.55 Impact Factor
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ABSTRACT: Monoclonal antibody D11-DG2 (DG2) against carcinoembryonic antigen (CEA) was examined for suitability for radioimmunodetection of human tumors grown in nude mice. Antibodies DG2 and a control antibody of the same IgG1 subclass were labeled with 131I and injected into mice bearing one of three types of CEA-containing tumors (cell lines LS 174T, HT-29 and Rec S) and/or a CEA-negative tumor (Rec R). Gamma-camera imaging and distribution studies revealed that CEA-containing tumors selectively accumulate DG2 but Rec R does not. As the tumors differ in CEA-content, the highest accumulation of 131I-DG2 (corresponding to the best scintigraphic imaging) was found in LS 174T tumors, intermediate in Rec S and lowest in HT-29 tumors. The mean tumor-to-blood ratios on the sixth day after antibody administration were 4.6, 3.2, and 2.1, respectively, in the control experiments the value of this parameter was always lower than 1. The results showed the applicability of DG2 for immunoscintigraphic studies in patients. Furthermore, a positive correlation was found between the uptake of anti-CEA antibody and CEA-content in the tumors.
Neoplasma 02/1990; 37(1):3-11. · 1.44 Impact Factor
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ABSTRACT: The authors present their experience gained in preparing, isolating and labeling antibodies with radionuclides for the purpose of using them in immunoscintigraphy. The experimental part includes results obtained with different labeled antibodies and their F/ab/2 fragments in distribution studies, involving also immunoscintigraphic imaging of tumors. The clinical part presents results of immunoscintigraphy obtained with the commercial antibody kits Iodomab and Imacis in patients with tumors of the digestive tract.
Bratislavske lekarske listy 11/1989; 90(10):740-3. · 0.40 Impact Factor
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ABSTRACT: In view of the reported association of Hodgkin's disease (HD) and ferritin, ferritin-bearing lymphocytes were followed during 2-year period in 79 HD patients. Indirect immunofluorescent method was used to evaluate the percentage of ferritin positive cells. In 22 untreated patients a high percentage of ferritin-bearing circulating lymphocytes (mean value 37.3%) was found. In regard to the extent of the disease higher values were found in clinical stage III and IV (mean value 40.6%) as compared to the stage I and II (mean value 26.2%). Similarly, 17 patients in relapse and with disease progression had mean values 41%. These proportions of cells were significantly lower in 44 patients in complete remission with mean value of 8.7% (60 examinations). In 30 healthy controls the mean value was 1.4%. Repeatedly performed examinations of ferritin-bearing lymphocytes during the follow-up period in 17 patients showed to be an important prognostic tool. A negative correlation of ferritin-bearing lymphocytes with E-rosette-forming cells was found. Iron content in peripheral blood lymphocytes was confirmed cytochemically after pre-incubation with antiferritin antibody. The results support the presumed role of ferritin in impaired cellular immunity in HD and suggest diagnostic and prognostic value of the examination of ferritin-bearing lymphocytes in HD.
Neoplasma 02/1986; 33(1):63-9. · 1.44 Impact Factor
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ABSTRACT: Mouse monoclonal antibodies to human urinary bladder carcinoma cells have been examined by indirect membrane immunofluorescence using a panel of 27 human cell lines. Two of the monoclonal antibodies, 7E9 (IgG3) and S2C6 (IgGl), were found to distinguish between urinary bladder carcinoma cells and normal urothelium. The third monoclonal antibody, T24.06.5(IgGl), discriminated among cell lines of urothelial and non-urothelial origin but did not distinguish between urinary bladder carcinoma and normal urothelial cells. None of the of the antibodies was found to be strictly selective, and occasional cross-reactions with unrelated cell types were observed. The monoclonal antibody 7E9, showing the highest degree of selectivity, was further examined by an indirect immunoperoxidase technique on frozen tissue sections from 19 patients. The antibody reacted with all (7/7) bladder carcinomas examined and gave negative results with control normal bladder mucosa (0/8) and unrelated tumor tissue (0/4) sections. The 7E9 antibody was purified by protein A affinity chromatography, labeled with 131I and used for gamma-scintigraphy in nude mice xenografted with human urinary bladder carcinoma T24. The 7E9 antibody was capable of locating the T24 xenografts in nude mice; it localized preferentially in the T24 tissue compared to normal mouse tissues. The T24 xenografts could not be detected by gamma-scintigraphy with 131I-labeled monoclonal antibody against human mammary carcinoma cells and two other control antibodies. Likewise the 131I-labeled 7E9 antibody was not capable of locating human mammary carcinoma xenografts in nude mice.
European Journal of Cancer and Clinical Oncology 07/1985; 21(6):701-10.
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ABSTRACT: Selectivity of mouse monoclonal antibody 7E9 (IGG3) directed against human urinary bladder carcinoma cells has been examined by indirect membrane immunofluorescence, using a panel of 31 human cell lines. The 7E9 monoclonal antibody discriminated between urinary bladder carcinoma cells and normal urothelium or cells of non-urothelial origin, although occasional reactions with bladder carcinoma-unrelated cell types were observed. The 7E9 antibody was purified by protein A affinity chromatography, labeled with 131I and used for gamma scintigraphy in nude mice xenografted with human urinary bladder carcinoma T24. The 7E9 antibody was capable of locating the T24 xenografts in nude mice; it localized preferentially in the T24 tissue compared to normal mouse tissues. The T24 xenografts could not be detected by gamma scintigraphy with 131I-labelled monoclonal antibody against human mammary carcinoma cells and two other control antibodies. Likewise, the 131I-labelled 7E9 antibody was not capable of locating human mammary carcinoma xenografts in nude mice.
Folia biologica 02/1985; 31(2):176-84. · 1.15 Impact Factor
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ABSTRACT: 131I-labelled antibodies to the fragment E of human fibrin, anti-rat fibrinogen, non-immune rabbit IgG and 67Ga-citrate were used for scintigraphy and distribution studies in experimental tumours in rats. The best visualisation was achieved with 131I-labelled anti-rat fibrinogen antibodies at intervals longer than 48 h after administration. Tumour to tissue ratios found in distribution studies performed formed more than 48 h after administration were higher in most examined tissues when using 131I-labelled antibodies than those obtained with 67Ga-citrate.
European Journal of Nuclear Medicine 02/1984; 9(10):478-81.
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Casopís lékar̆ů c̆eských 04/1983; 122(12):356-8.
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ABSTRACT: In 56 patients with Hodgkin's disease different clinical and histological stages the activity of polyphenoloxidase was repeatedly examined in the course of the disease as an indicator of serum ceruloplasmin concentration. In untreated patients the ceruloplasmin concentration was on average twice as high as in healthy controls. Repeated examinations made in the course of the disease demonstrated a good correlation of the values with the clinical condition of the patient, with the exception of some terminal stages and conditions following repeated chemotherapy, when liver damage was expected. The decrease to control levels in the course of therapy indicated the onset of remission. In contrast, increased levels indicated progression of the disease and necessity of treatment prolongation. Excluding intercurrent infections, higher ceruloplasmin levels above those found at remission were a clear sign of the progression. The correlation of ceruloplasmin values and erythrocyte sedimentation rate (1 h) was highly significant. The diagnostic and prognostic value of ceruloplasmin and/or serum copper determination is discussed.
Neoplasma 02/1982; 29(3):333-40. · 1.44 Impact Factor
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Casopís lékar̆ů c̆eských 11/1981; 120(39):1187-9.
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Vnitr̆ní lékar̆ství 03/1981; 27(2):195-200.
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Bratislavske lekarske listy 09/1980; 74(2):161-8. · 0.40 Impact Factor
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Sbornik lekarsky 04/1980; 82(3):89-96.
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Casopís lékar̆ů c̆eských 02/1978; 117(3):76-80.
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ABSTRACT: The authors examined a course of immunoglobulin values (IgA, IgG, IgM) in 40 patients with the Hodgkin's disease and repeatedly in 11 healthy persons. In the patients they followed the levels of the immunoglobulins during the disease, up to 5 years. In untreated patients there were statistically significant increased IgA levels and insignificant increase of IgG. In the course of remission the immunoglobulin values were normalized. The radiotherapy did not affect significantly the value of immunoglobulins, the combined chemotherapy (COPP) led to a drop of this level. The levels of immunoglobulins are recovered after chemotherapy in the case of successful treatment. In terms of the prognosis the authors consider as unfavorable high increase in the immunoglobulin levels in untreated patients and their rapid drop during the chemotherapy. They observed an average preterminal drop of the IgA by 27%, IgG by 23% and IgM by 44% with respect to controls.
Neoplasma 02/1978; 25(6):723-31. · 1.44 Impact Factor
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ABSTRACT: A suitable method of the isolation of alpha-1 fetoprotein for the needs of enzyme immunoassay of this oncofetal antigen is described. By combining isoelectric focusing and "indirect" affinity chromatography the preparation of alpha-1 fetoprotein was obtained that was not contaminated with IgG, contrary to the isolation performed by means of "direct" affinity chromatography on a carrier with coupled anti-alpha-1 fetoprotein antibodies, or other immunochemical methods that usually yielded contaminated preparations. Neither disc electrophoresis in PAA gel, immunoelectrophoresis, double radial immunodiffusion, nor biological experiments revealed any traces of ballast proteins in the resulting preparation; it seems suitable both for the preparation of monovalent antisera of a sufficient avidity, and as a standard for enzyme immunoassay.
Neoplasma 02/1978; 25(5):559-64. · 1.44 Impact Factor
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Sbornik lekarsky 04/1977; 79(3):89-96.
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Acta Universitatis Carolinae. Medica 02/1977; 23(5-6):321-30.
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Ceskoslovenská gynekologie 01/1977; 41(10):725-6.
