Joon Soo Park

Yonsei University Hospital, Sŏul, Seoul, South Korea

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Publications (48)53.37 Total impact

  • Joon Soo Park, Nam-In Goo, Dong-Eun Kim
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    ABSTRACT: Graphene oxide (GO) adsorbing a fluorophore-labeled single-stranded (ss) DNA serves as a sensor system, because subsequent desorption of the adsorbed probe DNA from GO in the presence of complementary target DNA enhances the fluorescence. In this study, we investigated the interaction of single- and double-stranded (ds) DNAs with GO by using a fluorescently labeled DNA probe. Although GO is known to preferentially interact with ssDNA, we found that dsDNA can also be adsorbed on GO, albeit with lower affinity. Furthermore, the status of ssDNA or dsDNA previously adsorbed on the GO surface was investigated by adding complementary or noncomplementary DNA (cDNA or non-cDNA) to the adsorption complex. We observed that hybridization occurred between the cDNA and the probe DNA on the GO surface. Based on the kinetics driven by the incoming additional DNA, we propose a mechanism for the desorption of the preadsorbed probe DNA from the GO surface: the desorption of the GO-adsorbed DNA was facilitated following its hybridization with cDNA on the GO surface; when the GO surface was almost saturated with the adsorbed DNA, nonspecific desorption dominated the process through a simple displacement of the GO-adsorbed DNA molecules by the incoming DNA molecules because of the law of mass action. Our results can be applied to design appropriate DNA probes and to choose proper GO concentrations for experimental setups to improve specific signaling in many biosensor systems based on the GO platform.
    Langmuir : the ACS journal of surfaces and colloids. 10/2014;
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    ABSTRACT: Purpose To compare the transportal (TP) and outside-in (OI) techniques regarding femoral tunnel position and geometry after anatomic single-bundle (SB) anterior cruciate ligament (ACL) reconstruction. Methods This study included 51 patients who underwent anatomic SB ACL reconstruction with the TP (n = 21) or OI (n = 30) technique. All patients underwent 3-dimensional computed tomography 3 days after the operation. The femoral tunnel position (quadrant method), femoral graft bending angle, femoral tunnel length, and posterior wall breakage were assessed by immediate postoperative 3-dimensional computed tomography with OsiriX imaging software. Results The OI technique had a shallower femoral tunnel position (arthroscopic position) than did the TP technique (P = .005). The mean femoral graft bending angle was significantly more acute with the OI technique (101.3° ± 8.2°) than with the TP technique (107.9° ± 10.0°) (P = .02). The mean femoral tunnel length was significantly greater with the OI technique (33.0 ± 3.5 mm) than with the TP technique (29.6 ± 3.9 mm) (P = .003). Posterior wall breakage occurred in 7 cases (33.3%) with the TP technique and 1 case (3.3%) with the OI technique (P = .02). Conclusions The mean femoral tunnel position was significantly shallower (arthroscopic position) with the OI technique than with the TP technique. The OI technique resulted in a more acute femoral graft bending angle, longer femoral tunnel length, and lower incidence of posterior wall breakage than did the TP technique. These results might be helpful for anatomic SB ACL reconstruction using TP and OI techniques. Level of Evidence Level III, retrospective comparative study.
    Arthroscopy The Journal of Arthroscopic and Related Surgery 01/2014; · 3.10 Impact Factor
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    ABSTRACT: Live bee acupuncture (Bong-Chim) dermatitis is an iatrogenic disease induced by so-called live bee acupuncture therapy, which applies the honeybee (Apis cerana) stinger directly into the lesion to treat various diseases in Korea. We present two cases of live bee acupuncture dermatitis and review previously published articles about this disease. We classify this entity into three stages: acute, subacute, and chronic. The acute stage is an inflammatory reaction, such as anaphylaxis or urticaria. In the chronic stage, a foreign body granuloma may develop from the remaining stingers, similar to that of a bee sting reaction. However, in the subacute stage, unlike bee stings, we see the characteristic histological "flame" figures resulting from eosinophilic stimulation induced by excessive bee venom exposure. We consider this stage to be different from the adverse skin reaction of accidental bee sting.
    International journal of dermatology 10/2013; · 1.18 Impact Factor
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    ABSTRACT: We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface.
    Chemical Communications 08/2013; · 6.38 Impact Factor
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    ABSTRACT: Graphene oxide (GO) is known to interact with single-stranded nucleic acids through pi-stacking interactions and hydrogen bonds between the nucleobases and the hexagonal cells of GO. It also quenches the fluorescence when the fluorophore comes near to the GO mesh. When single-stranded (ss) regions of either DNA or RNA are present, those regions were adsorbed onto the surface of GO with a quenching of fluorescence located proximally to the GO surface. We demonstrated that bound single-stranded nucleic acids can be readily dissociated from GO by disrupting hydrogen bonding with urea, which was confirmed with fluorescence measurement and gel electrophoresis. Hydrogen bonding mainly contributes to the interaction between GO and single-stranded nucleic acids such as ssDNA and RNA. The GO-coated mesoporous silica nanoparticles (GO-MSNs) were synthesized for better separation of RNAs from cells. Cellular RNAs were readily adsorbed and eluted with ease by using GO-MSN and urea, respectively, demonstrating that GO-MSN and urea elution is a facile RNA extraction method.
    The Analyst 01/2013; · 4.23 Impact Factor
  • Joon Soo Park, Dong Kyun Ko
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    ABSTRACT: BACKGROUND: An epidermoid cyst is a common epithelial-lined cyst. There have been many studies on epidermoid cysts, but few focused on ruptured epidermoid cyst and its histopathologic characteristics. OBJECTIVE: We evaluated the histopathologic differences between ruptured and unruptured epidermoid cysts, and their relationships. METHODS: We retrospectively examined 359 excision biopsy specimen diagnosed as epidermoid cyst from 1991 to 2011 at Department of dermatology at Daegu Catholic University Hospital. RESULTS: The mean cyst area was 38.89 mm(2) and the mean cyst area of the unruptured group was larger than that of the ruptured group. The mean wall thickness was 90.15 μm and was thicker in ruptured group than in unruptured group. The correlation between cyst diameter and wall thickness had statistically negative correlation in unruptured and in ruptured group. In ruptured group, the cystic size of the cases with rete ridge was smaller than that of without rete ridge. The cyst wall thickness of the cases with rete ridge was thicker than that of the cases without rete ridge. LIMITATIONS: For comparative evaluation of sizes, randomly shaped cysts are assumed to be perfectly elliptic. And only those with more than 3/4 cystic wall remaining were included in the subject. CONCLUSION: When comparing the ruptured and the unruptured cyst, the rupture of cyst had significant relationship with increased cyst diameter and area, increased wall thickness, more cyst contents, and more wall changes. Moreover, the presence of rete ridge in ruptured cystic wall is a valuable variable to recognize the duration of the rupture.
    International journal of clinical and experimental pathology 01/2013; 6(2):242-248. · 2.24 Impact Factor
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    ABSTRACT: BMI nozzles in bottom head of the nuclear vessel are one of major components in nuclear power plants. The BMI nozzles have high possibility to generate PWSCC(primary water stress corrosion crack) according to recent foreign case although operation temperature is lower then the upper head of the nuclear vessel. Thus, nondestructive inspection of the BMI nozzles is required. But, inspection of BMI nozzles is not easy since the BMI nozzles placed in high radiated area and inside the nozzles filled with boric acid. Thus, in this study, a TOFD transducer for inspection of BMI and automated scanner system with water were developed. Also, validation of performance of the developed transducer and system are performed using specimens with artificial defects.
    Journal of the Korean Society for Nondestructive Testing. 01/2013; 33(1).
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    ABSTRACT: Abstract is missing (Quiz).
    Acta Dermato-Venereologica 11/2012;
  • International journal of dermatology 11/2012; 51(11):1388-9. · 1.18 Impact Factor
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    Kwi Sung Park, Young Jin Choi, Joon Soo Park
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    ABSTRACT: Although most enterovirus infections are not serious enough to be life threatening, several enteroviruses such as enterovirus 71 are responsible for severe, potentially life-threatening disease. The epidemic patterns of enteroviruses occur regularly during the year, but they may change due to environmental shifts induced by climate change due to global warming. Therefore, enterovirus epidemiological studies should be performed continuously as a basis for anti-viral studies. A great number of synthesized antiviral compounds that work against enteroviruses have been developed but only a few have demonstrated effectiveness in vivo. No proven effective antiviral agents are available for enterovirus disease therapy. The development of a new antiviral drug is a difficult task due to poor selective toxicity and cost. To overcome these limitations, one approach is to accelerate the availability of other existing antiviral drugs approved for antiviral effect against enteroviruses, and the other way is to screen traditional medicinal plants.
    Korean Journal of Pediatrics 10/2012; 55(10):359-66.
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    ABSTRACT: A peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR™ TB/NTM detection kit; PANAGENE, Korea) assay has been recently developed for the simultaneous detection of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM) in clinical specimens. The study was aimed at evaluation of the performance of PNA probe-based real-time PCR in respiratory specimens. To evaluate potential cross-reactivity, the extracted DNA specimens from Mycobacterium species and non-mycobacterial species were tested using PNA probe-based real-time PCR assay. A total of 531 respiratory specimens (482 sputum specimens and 49 bronchoalveolar washing fluid specimens) were collected from 230 patients in July and August, 2011. All specimens were analyzed for the detection of mycobacteria by direct smear examination, mycobacterial culture, and PNA probe-based real-time PCR assay. In cross-reactivity tests, no false-positive or false-negative results were evident. When the culture method was used as the gold standard test for comparison, PNA probe-based real-time PCR assay for detection of MTBC had a sensitivity and specificity of 96.7% (58/60) and 99.6% (469/471), respectively. Assuming the combination of culture and clinical diagnosis as the standard, the sensitivity and specificity of the new real-time PCR assay for detection of MTBC were 90.6% (58/64) and 99.6% (465/467), respectively. The new real-time PCR for the detection of NTM had a sensitivity and specificity of 69.0% (29/42) and 100% (489/489), respectively. The new real-time PCR assay may be useful for the detection of MTBC in respiratory specimens and for discrimination of NTM from MTBC.
    Annals of Laboratory Medicine 07/2012; 32(4):257-63. · 1.48 Impact Factor
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    ABSTRACT: Since the production of various vaccine formulations by different pharmaceutical companies and interruptions in vaccine supply cannot be fully regulated, problems caused by DTaP interchangeability may occur. However, the interchangeability data on the first primary series of DTaP vaccination have not been well described. In this study we investigated immunogenicity and overall safety of diphtheria, tetanus, pertussis in children who had adventitiously received an interchangeable DTaP vaccination. A total 97 of participants were enrolled (mean age, 11 months). High immunogenicity (1.8 ± 0.4 IU/mL, 100%) was noted against diphtheria toxoid, and similar high immunogenicity (3.2 ± 2.1 IU/mL, 100%) was noted against tetanus toxoid. Immunogenicity against pertussis antigen was significantly greater in the interchangeable vaccinated group compared to the control group, and 57% of the interchangeable vaccinated subjects achieved high levels of protective immunity (45.2 ± 21.5 E U/mL). No serious adverse effects were noted, and most adverse effects resolved without treatment. The immunogenicity against each antigen was high in patients who were interchangeably vaccinated for DTaP. Our results provide clinical evidence affirming the non-inferiority of interchangeable vaccination when it cannot be avoided such as in limited vaccine supply situations or different prices.
    Vaccine 05/2012; 30(31):4644-7. · 3.77 Impact Factor
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    ABSTRACT: Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
    Annals of Laboratory Medicine 01/2012; 32(1):79-81. · 1.48 Impact Factor
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    ABSTRACT: Pemphigus vulgaris is an autoimmune blistering disease that commonly involves the scalp. Lesions of pemphigus vulgaris that persist on the scalp for a long period may be accompanied by tufted hair folliculitis. Only two previous accounts of tufted hair folliculitis developing in a lesion of pemphigus vulgaris have been reported. We report a 51-year-old-man with erosions and clusters of hair on the scalp. The scalp lesion had persisted for about 20 years. A histopathological examination of the skin lesion on the scalp revealed separation of the epidermis and clusters of several adjacent hair follicles. The patient was diagnosed with persistent pemphigus vulgaris of the scalp showing features of tufted hair folliculitis.
    Annals of Dermatology 11/2011; 23(4):523-5. · 0.61 Impact Factor
  • Dermatitis 11/2011; 22(6):354-7. · 0.93 Impact Factor
  • Journal of the American Academy of Dermatology 07/2011; 65(1):247-9. · 4.91 Impact Factor
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    ABSTRACT: A major problem of long-term antiviral therapy in chronic hepatitis B patients is the emergence of hepatitis B virus (HBV) mutations associated with drug resistance. Recently, a new array using peptide nucleic acids (PNAs), which are synthetic nucleic acid analogues, was developed for the detection of HBV mutations at six different codon positions associated with lamivudine (LAM) and adefovir (ADV) resistance. We compared the PNA array with direct sequencing and reverse hybridization (INNO-LiPA) in 73 samples obtained from chronic hepatitis B patients. The PNA array detected mutations associated with LAM and/or ADV resistance in 60 (82.2%) of the 73 samples. The overall concordance rate of PNA array and INNO-LiPA compared with direct sequencing was 99.5% and 98.2%, respectively. The rate of complete concordance between PNA array and INNO-LiPA was 92.7%. The PNA array assay results were comparable with INNO-LiPA for detection of HBV mutations associated with antiviral resistance.
    Archives of Virology 05/2011; 156(9):1517-24. · 2.28 Impact Factor
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    ABSTRACT: Lowe syndrome is a rare genetic disease that appears to cause various clinical symptoms involving the eye, nervous system, and kidney. While a mutation of the OCRL1 gene is known to be responsible for this syndrome, the exact pathophysiology remains unclear. Various multi-organ symptoms are characteristic of Lowe syndrome, but skin lesions have rarely been described. Recently, mechanisms for the association of Lowe syndrome and skin lesions have been proposed. We report this case of Lowe syndrome involving multiple epidermal cysts on the scalp in a 6-year-old male child.
    Annals of Dermatology 11/2010; 22(4):444-6. · 0.61 Impact Factor
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    ABSTRACT: Multiple endocrine neoplasia type 2B (MEN 2B) is a rare disease caused by germline mutations in the RET proto-oncogene and is transmitted in an autosomal dominant fashion. It is characterized by medullary thyroid carcinoma, pheochromocytoma and mucosal neuroma developing in the tongue, lip, intestinal tract, palate etc. Among these neoplasias, mucosal neuroma generally develops from early childhood. Therefore, early detection and proper treatment can minimize the disease course. Here we describe a 9-year-old male who presented with multiple verrucous papules and nodules on his lips, tongue and gingiva that were there since birth. Histologic findings of his lips and tongue showed well-defined nerve bundles and DNA analysis revealed a M918T mutation at codon 918 of the RET oncogene. He was diagnosed early as having MEN 2B according to his genetic and phenotypic features.
    Annals of Dermatology 11/2010; 22(4):452-5. · 0.61 Impact Factor

Publication Stats

110 Citations
53.37 Total Impact Points

Institutions

  • 2014
    • Yonsei University Hospital
      Sŏul, Seoul, South Korea
  • 2013
    • Konkuk University
      • Department of Bioscience and Technology
      Seoul, Seoul, South Korea
  • 2008–2013
    • Catholic University of Daegu
      • • College of Medicine
      • • Department of Medicine
      • • Department of Internal Medicine
      Hayang, North Gyeongsang, South Korea
  • 2009
    • Korea University
      • College of Medicine
      Seoul, Seoul, South Korea
  • 2004
    • Sungkyunkwan University
      • School of Mechanical Engineering
      Seoul, Seoul, South Korea