Robert Srnec

University Hospital Brno, Brno, South Moravian Region, Czech Republic

Are you Robert Srnec?

Claim your profile

Publications (16)8.99 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.
    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 06/2012; 156(2):128-34. · 0.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To quantify humeroulnar incongruity on elbow radiographs in Labrador Retrievers with or without medial coronoid disease (MCD). Retrospective study of 92 elbows. Radiographic projections of elbow joints from Labrador Retrievers with MCD (n = 42 elbows; 26 dogs) and without MCD (n = 50 elbows; 25 dogs). The congruity of the humeroulnar joint was measured using an index of subluxation (SI) for each elbow. SI was defined as the distance between the centers of 2 circles drawn along the margins of the incisura trochlearis and the trochlea of humerus on mediolateral digital radiographic projections, normalized by the radius of the circle circumscribing the humeral trochlea. SI was compared between right and left elbows with and without pathology using a Wilcoxon test for paired data, and between normal and abnormal groups with a Wilcoxon test for unpaired data. Mismatch between ulnar curvature and curvature of humeral trochlea and radioulnar incongruency were also noted (Wilcoxon test). The intraobserver repeatability, correlation between SI and radioulnar incongruency, and between SI and mismatch elbow curvature were estimated with a Pearson's correlation coefficient. Intraobserver repeatability of SI measurement was high (r = 0.97). Mean ± SD humeroulnar incongruity (SI) was greater in elbows with MCD (18.5 ± 6.6) than in the normal elbows (1.7 ± 2.0, P < 0.001). The difference between the diameters of the curvatures of the ulnar and humeral trochlea was greater in elbows with MCD (12.5 ± 4.4) than in the normal group (10.7 ± 4.1, P < 0.05). A moderate correlation was found between the degree of humeroulnar incongruity and a radioulnar step (r = 0.63); however, no correlation was identified between SI and the difference between the diameters of the curvatures of the ulnar and humeral trochleae (r = 0.14). We propose a radiographic index to measure humeroulnar incongruity on mediolateral digital radiographic projections. This index (SI) supports the presence of humeroulnar incongruity in Labrador Retrievers with MCD. Further evaluation of its reproducibility and clinical importance are warranted. Although there is a moderate correlation between humeroulnar incongruity and radioulnar incongruency, causation has not been established.
    Veterinary Surgery 10/2011; 40(8):981-6. · 1.24 Impact Factor
  • Source
    Acta Veterinaria Brno - ACTA VET BRNO. 01/2010; 79(2):307-312.
  • Acta Veterinaria Brno - ACTA VET BRNO. 01/2010; 79(2):299-306.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To compare the incidence of seizures in dogs with intervertebral disk disease after iopamidol or iomeprol myelography, and to assess whether the incidence of seizures differed between the 2 agents when severity of neurological deficits, location of cord compression, duration of anesthesia, site of myelogram, volume of contrast, and concentration of contrast were evaluated. Retrospective study. Veterinary teaching hospital. One hundred and sixty-one client-owned dogs with intervertebral disk disease. Subarachnoid injection of contrast medium. One hundred and sixty-one dogs with intervertebral disk disease were subjected to myelography using iopamidol (n=74) or iomeprol (n=87). Cranial myelography was performed in 31 dogs, caudal myelography in 125 and both cranial and caudal myelography in 5. Seizures occurred in 23 of 161 (14%) dogs. There was no significant difference overall between iopamidol and iomeprol myelography. However, in dogs with thoracolumbar disk extrusion and paraplegia, seizures occurred more frequently after caudal myelography using iopamidol compared with iomeprol. Both iomeprol and iopamidol are suitable for myelography in dogs. Iomeprol is recommended for caudal myelography in paraplegic dogs with thoracolumbar disk extrusion due to the higher incidence of seizures in this group when iopamidol was used.
    Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001). 12/2009; 19(6):611-6.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to evaluate macroscopically, histologically and immunohistochemically the quality of newly formed tissue in iatrogenic defects of articular cartilage of the femur condyle in miniature pigs treated with the clinically used method of microfractures in comparison with the transplantation of a combination of a composite scaffold with allogeneic mesenchymal stem cells (MSCs) or the composite scaffold alone. The newly formed cartilaginous tissue filling the defects of articular cartilage after transplantation of the scaffold with MSCs (Group A) had in 60 % of cases a macroscopically smooth surface. In all lesions after the transplantation of the scaffold alone (Group B) or after the method of microfractures (Group C), erosions/fissures or osteophytes were found on the surface. The results of histological and immunohistochemical examination using the modified scoring system according to O'Driscoll were as follows: 14.7+/-3.82 points after transplantations of the scaffold with MSCs (Group A); 5.3+/-2.88 points after transplantations of the scaffold alone (Group B); and 5.2+/-0.64 points after treatment with microfractures (Group C). The O'Driscoll score in animals of Group A was significantly higher than in animals of Group B or Group C (p<0.0005 both). No significant difference was found in the O'Driscoll score between Groups B and C. The treatment of iatrogenic lesions of the articular cartilage surface on the condyles of femur in miniature pigs using transplantation of MSCs in the composite scaffold led to the filling of defects by a tissue of the appearance of hyaline cartilage. Lesions treated by implantation of the scaffold alone or by the method of microfractures were filled with fibrous cartilage with worse macroscopic, histological and immunohistochemical indicators.
    Physiological research / Academia Scientiarum Bohemoslovaca 11/2009; 59(4):605-14. · 1.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This synoptic study gives a concise overview of current knowledge of bone healing, the role of mesenchymal stem cells in bone tissue regeneration and contemporary possibilities of supporting regeneration of damaged bone. Attention of research concerning the healing of fractures with extensive loss of bone tissue following trauma, the treatment of belatedly healing or non-healing fractures or the healing of segmental bone defects following tumour resection, is focused on development of three-dimensional scaffolds planted with mesenchymal stem cells that might be used for reconstruction of such large bone lesions. Presented are possibilities of transplantation of mesenchymal stem cells combined with biomaterials into bone defects, including the results of our own experimental studies dealing with the use of stem cells in the treatment of damaged tissues of the musculoskeletal system in animal models.
    Acta Veterinaria Brno - ACTA VET BRNO. 01/2009; 78(4):635-642.
  • Source
    Acta Veterinaria Brno - ACTA VET BRNO. 01/2009; 78(4):643-648.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study appears from an experiment previously carried out in New Zealand white rabbits. Allogenic mesenchymal stem cells (MSCs) were transplanted into an iatrogenically-created defect in the lateral section of the distal physis of the left femur in 10 miniature pigs. The right femur with the same defect served as a control. To transfer MSCs, a freshly prepared porous scaffold was used, based on collagen and chitosan, constituting a compact tube into which MSCs were implanted. The pigs were euthanized four months after the transplantation. On average, the left femur with transplanted MSCs grew more in length (0.56+/-0.14 cm) compared with right femurs with physeal defect without transplanted MSCs (0.14+/-0.3 cm). The average angular (valgus) deformity of the left femur had an angle point of 0.78 degrees , following measurement and X-ray examination, whereas in the right femur without transplantation it was 3.7 degrees. The initial results indicate that preventive transplantation of MSCs into a physeal defect may prevent valgus deformity formation and probably also reduce disorders of the longitudinal bone growth. This part of our experiment is significant in the effort to advance MSCs application in human medicine by using pig as a model, which is the next step after experimenting on rabbits.
    Physiological research / Academia Scientiarum Bohemoslovaca 01/2009; 58(6):885-93. · 1.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the study was to verify whether there is a difference in the lengthwise growth of the femurs and in their angular deformity when comparing preventive vs. therapeutic transplantation of allogeneic mesenchymal stem cells (MSCs) to an iatrogenic defect in the distal physis of femur. Modified composite chitosan/collagen type I scaffold with MSCs was transplanted to an iatrogenically created defect of the growth cartilage in the lateral condyle of the left femur in 20 miniature male pigs. In Group A of animals (n = 10) allogeneic MSCs were transplanted immediately after creating the defect in the distal physis of femur (preventive transplantation). In Group B of animals (n = 10) the same defect of the growth cartilage was treated by transplantation of allogeneic MSCs four weeks after its creation (therapeutic transplantation), after the excision of the bone bridge that had formed in it. On average, left femurs with a damaged distal physis and preventively transplanted allogeneic MSCs (Group A) grew during 4 months from transplantation by 0.56 ± 0.44 cm more than right femurs without the transplantation of MSCs, whereas left femurs with physeal defects treated with a therapeutic transplantation of allogeneic MSCs (Group B) by 0.14 ± 0.72 cm only, compared to right femurs without transplanted MSCs. Four months after preventive transplantation of MSCs (Group A), valgus deformity of the distal part of left femur with the defect was 0.78 ± 0.82°; in the control group (right femur in the same animal without MSCs transplantation) it was 3.7 ± 0.82°. After therapeutic transplantation of MSCs (Group B) 0.6 ± 3.4°, in the control group (right femur in the same animal without MSCs transplantation) it was 2.1 ± 2.9°. In all the animals of Groups A and B, the presence of newly formed hyaline cartilage was confirmed histologically and immunohistochemically. In the distal physis of right femurs with an iatrogenic defect of the growth cartilage without the transplantation of MSCs (control) bone bridge was formed. Preventive transplantation of allogeneic MSCs into the defect of the distal growth zone of femur appears more suitable compared to the therapeutic transplantation, with regard to the more pronounced lengthwise bone growth. Differences found in the extent of valgus deformity were non-significant comparing preventive and therapeutic transplantations of MSCs. MSCs, growth plate, deformity, bone bridge Injury of the growth cartilage of the long bones of the extremities usually leads to the formation of a bone bridge between epiphysis and metaphysis of the long bone (Key and Ford 1958; Bright 1974). At the same time, in approximately 25 - 30% of cases the lengthwise bone growth is reduced and its angular deformity occurs (Ahn et al. 2004). Besides, in 10% of these cases, the function of the relevant joint is impaired (Mann and
    Acta Veterinaria Brno - ACTA VET BRNO. 01/2009; 78(2):293-302.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this experimental study on New Zealand's white rabbits was to find differences in the results of treating the distal physeal femoral defect by the transplantation of autologous or allogeneic mesenchymal stem cells (MSCs). After the excision of a created bone bridge in the distal physis of the right femur, modified composite scaffold with MSCs was transplanted into the defect. In animal Group A (n = 11) autogenous MSCs were implanted; in animal Group B (n = 15) allogeneic MSCs were implanted. An iatrogenic physeal defect of the left femur of each animal not treated by MSCs transplantation served as control. The rabbits were euthanized four months after the transplantation. The treatment results were evaluated morphometrically (femoral length and valgus deformity measurement) and histologically (character and quality of the new cartilage). Four months after the transplantation, the right femurs of the animals in Group A were on average longer by 0.50 +/- 0.04 cm (p = 0.018) than their left femurs, the right femurs of rabbits in Group B were on average longer by 0.43 +/- 0.01 cm (p = 0.028) than their left femurs.4 months after the therapeutic transplantation of MSCs valgus deformity of the distal part of the right femur of animals in Group A was significantly lower (by 4.45 +/- 1.86 degrees ) than that of their left femur (p = 0.028), in Group B as well (by 3.66 +/- 0.95 degrees than that of their left femur p = 0.001). However, no significant difference was found between rabbits with transplanted autogenous MSCs (Group A) and rabbits with transplanted allogeneic MSCs (Group B) either in the femur length (p = 0.495), or in its valgus deformity (p = 0.1597). After the MSCs transplantation the presence of a newly formed hyaline cartilage was demonstrated histologically in all the animals (both groups). The ability of transplanted MSCs to survive in the damaged physis was demonstrated in vivo by magnetic resonance, in vitro by Perls reaction and immunofluorescence. The transplantation of both autogenous and allogeneic MSCs into a defect of the growth plate appears as an effective method of surgical treatment of physeal cartilage injury. However, the Findings point to the conclusion that there is no clear difference in the final effect of the transplantation procedure used.
    BMC Biotechnology 10/2008; 8:70. · 2.17 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Articular cartilage trauma, in particular due to its poor healing potential remains a complicated problem in both the adult and paediatric traumatology and orthopedics. In older patients, total endoprosthesis of the joint is a method of choice, however, in younger patients, the situation remains more complicated. In case of osteochondral lesions (arthrosis, chondral fractures. osteochoodrosis dissecns) the ideal management should result in complete recovery of the hyaline cartilage on the traumatized joint surface. Contemporary medicine uses some therapeutic procedures resulting in partial recovery of the articular cartilage structure at the lesion site and several techniques of excisionining the articular surface's injured part and of transplantations of biological grafts. Regarding the above first approach, abrasive methods (micro fractures, small drill holes), which are expected to result in recovery of the articular cartilage through progenitor cells that migrate from the bone marrow to the defect site following subchondral fracturing. In case the injury is managed early, the osteochondral fragment may be fixed and the articular congruence be recovered. Mosaicoplasty using osteochondral auto grafts or other autologous grafts, or more recently using transplantations of autologous chond rocytes, which seem to have a major potential in the hyaline cartilage healing process. However, methodology of the transplant retention at the defect site remains a problem. Furthermore, the use of mesenchymal stem cells, so far in the experimental phase, appears prospective. Pivotal articular cartilage treatment research activities have progressed to a level of searching for a suitable scaffold of perfect qualities. This is the task for cooperation with bioengineering. requiring provision of the most exact differentiation protocol for hyline cartilage producing mesenchymal stem cells (MSCs).
    Rozhledy v chirurgii: měsíčník Československé chirurgické společnosti 02/2008; 87(1):42-5.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Department of surgery and Orthopaedics, small Animal clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical sciences Brno, czech Republic 2 Department of Pediatric surgery, Orthopaedics and Traumatology, Faculty hospital Brno, czech Republic Abstract: Biomaterials and stem cells in the Treatment of Articular cartilage, Meniscal, Physeal, Bone, Ligamentous and Tendineous Defects. Acta Vet Brno 2008, 77: 277-284. This review briefly summarizes existing results and main trends in experimental studies dealing with the possibilities of the use of biomaterials and mesenchymal stem cells in the treatment of diseases affecting tissues derived from the mesoderm, i.e. articular cartilage, meniscus, physis, bone, ligaments and tendons. The aim of these experimental studies is to find optimal forms of treatment of the diseases of individual parts of the musculoskeletal system using methods of tissue engineering with the use of stem cell transplantation.
    Acta Veterinaria Brno - ACTA VET BRNO. 01/2008; 77(2).
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Changes of Vital Parameters after Administration of Butorphanol during Tiletamine-Zolazepam-Ketamine-Xylazine Anaesthesia for Joint Surgery in Miniature Pigs. Acta Vet. Brno 2008, 77: 251-256. The study compares the effects of butorphanol in pigs undergoing joint surgery in tiletamine-zolazepam-ketamine-xylazine (TKX) anaesthesia. A total of 12 pigs were divided into 2 groups by 6 animals -BUT (anaesthetized with TKX combination and butorphanol) and CON (control group -anaesthetized with TKX combination only). All pigs were sedated with a mix of tiletamin-zolazepam-ketamin-xylazin, put into total anaesthesia using propofol, and connected to an anaesthesiology unit (O 2 -Air). For 40 min we logged the heart rate (HR), respiratory rate (RR), mean arterial pressure (MAP), haemoglobin saturation by oxygen (SpO 2) and end-tidal CO 2 concentration (ETCO 2) values. Ten minutes after connecting to the devices, the pigs in the BUT group were intravenously administered butorphanol (0.2 mg/kg) in the total volume of 2 ml, or physiological saline in the same volume. The pigs in the BUT group had a lower (p < 0.05) HR in 5 th , 10 th and 25 th min, and a lower RR in the 10 th , 15 th and 20 th min. MAP, ETCO 2 and SpO 2 values did not differ substantially. Butorphanol can thus be identified as a suitable analgesic TKX supplement to anaesthesia of miniature pigs with minimum effect on vital functions.
    Acta Veterinaria Brno - ACTA VET BRNO. 01/2008; 77(2).
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Computed tomography (CT) is an effective diagnostic modality for three-dimensional imaging of bone structures, including the geometry of their defects. The aim of the study was to create and optimize 3D geometrical and real plastic models of the distal femoral component of the knee with joint surface defects. Input data included CT images of stifle joints in twenty miniature pigs with iatrogenic osteochondrosis-like lesions in medial femoral condyle of the left knee. The animals were examined eight and sixteen weeks after surgery. Philips MX 8000 MX and View workstation were used for scanning parallel plane cross section slices and Cartesian discrete volume creation. On the average, 100 slices were performed in each stifle joint. Slice matrices size was 512 x 512 with slice thickness of 1 mm. Pixel (voxel) size in the slice plane was 0.5 mm (with average accuracy of +/-0.5 mm and typical volume size 512 x 512 x 100 voxels). Three-dimensional processing of CT data and 3D geometrical modelling, using interactive computer graphic system MediTools formerly developed here, consisted of tissue segmentation (raster based method combination and 5 % of manual correction), vectorization by the marching-cubes method, smoothing and decimation. Stifle- joint CT images of three individuals of different body size (small, medium and large) were selected to make the real plastic models of their distal femurs from plaster composite using rapid prototyping technology of Zcorporation. Accuracy of the modeling was +/- 0.5 mm. The real plastic models of distal femurs can be used as a template for developing custom made press and fit scaffold implants seeded with mesenchymal stem cells that might be subsequently implanted into iatrogenic joint surface defects for articular cartilage-repair enhancement.
    Physiological research / Academia Scientiarum Bohemoslovaca 02/2007; 56 Suppl 1:S107-14. · 1.53 Impact Factor
  • Source
    A. NEâAS, R. SRNEC, H. KECOVÁ
    [Show abstract] [Hide abstract]
    ABSTRACT: Neãas A., R. Srnec, H. Kecová:Diagnostic Reliability of Stifle Arthroscopy of Pathological Changes in Cruciate Deficient Knee. Acta Vet. Brno 2002, 71: 249-254. Arthroscopy is becoming a modern mini-invasive method of diagnosis and therapy of cranial cruciate ligament rupture in the stifle of the dog. This study was engaged in the arthroscopically assisted diagnosis and therapy of 42 cases of the cranial cruciate ligament rupture. Every arthroscopic operation was turned to arthrotomy for the purpose of verifying the correctness of findings. Comparing stifle arthrotomy and arthroscopy, the diagnostic accuracy of arthroscopic findings ranged in dependence on the kind of lesion and intraarticular tissue affected in the stifle with CCL rupture from 92 to 100%. Hundred percent reliability of arthroscopic findings was noted when evaluating the integrity of the cranial cruciate ligament. The diagnosis was wrong in two out of 25 cases of medial meniscal damage. Arthroscopic evaluation of the articular cartilage and synovial membrane was very precise and more detailed than during arthrotomy. Arthroscopy, after routine mastering of the technique, can thus be a reliable diagnostic method ensuring mini-invasiveness of evaluation of intraarticular structures in the stifle affected with CCL rupture. Cranial cruciate ligament, meniscus, dog
    01/2002;

Publication Stats

33 Citations
8.99 Total Impact Points

Institutions

  • 2008–2009
    • University Hospital Brno
      • Department of Pediatric Surgery, Orthopedics and Traumatology
      Brno, South Moravian Region, Czech Republic
    • Brno University of Technology
      • Institute of Materials Chemistry "IMC"
      Brünn, South Moravian, Czech Republic
  • 2007–2009
    • University of Veterinary and Pharmaceutical Sciences Brno
      • Faculty of Veterinary Medicine
      Brno, South Moravian Region, Czech Republic