Kee-Young Kim

Publications (3)11.45 Total impact

• Article: Molecular cloning of low-temperature-inducible ribosomal proteins from soybean.

  Kee-Young Kim, Seong-Whan Park, Young-Soo Chung, Chung-Han Chung, Jung-In Kim, Jai-Heon Lee
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  ABSTRACT: Three ribosomal protein genes induced by low-temperature treatment were isolated from soybean. GmRPS13 (742 bp) encodes a 17.1 kDa protein which has 95% identity with the 40S ribosomal protein S13 of Panax ginseng (AB043974). GmRPS6 (925 bp) encodes a 28.1 kDa protein which has 94% identity with the 40S ribosomal protein S6 of Asparagus officinalis (AJ277533). GmRPL37 (494 bp) encodes a 10.7 kDa protein which has 85% identity with the 60S ribosomal protein L37 of Arabidopsis thaliana (AF370216). The expression of these ribosomal protein genes started to increase 3 d after low-temperature treatment, whereas the cold-stress protein src1 was highly induced from the first day. Such late response of these ribosomal protein genes may be due to secondary signals during cold adaptation. The induction of ribosomal protein genes might enhance the translation process or help proper ribosome functioning under low-temperature conditions.
  Journal of Experimental Botany 06/2004; 55(399):1153-5. · 5.36 Impact Factor
• Article: Flow karyotypes and chromosomal DNA contents of genus Triticum species and rye (Secale cereale).

  Jai-Heon Lee, Youzhi Ma, Toshiyuki Wako, Lian Cheng Li, Kee-Young Kim, Seong-Whan Park, Susumu Uchiyama, Kiichi Fukui
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  ABSTRACT: The flow cytometry and chromosome imaging method were jointly used for analyzing genome content and chromosomal DNA content of hexaploid wheat (AABBDD), hexaploid triticale (AABBRR), tetraploid wheat (AABB), and AA, BB, DD genome donors and RR genome rye. Their genome sizes were 34.4 pg, 40.9 pg, 26.2 pg, 12.1 pg, 13.7 pg, 10.5 pg, and 16.9 pg, respectively. The 2C nuclear DNA content of BB genome donor with 13.7 pg was the highest value among the other genome donors, AA or DD. The genome content of tetraploid wheat, unlike hexaploid wheat or hexaploid triticale, was larger than the sum of the genomes of AA and BB genome donors. The DNA content of each chromosome ranged from 1.22 pg in DD genome donor to 2.61 pg in rye. Each chromosome peak was divided into three to four groups. Only one chromosome was included in the highest chromosomal DNA peak in hexaploid wheat, tetraploid wheat, DD genome donor and rye but two chromosomes in AA, BB genome donors, and hexaploid triticale. Correlation between 2C nuclear DNA content and chromosome density volume was the highest value compared with the other chromosomal parameters of chromosome area, or chromosome length.
  Chromosome Research 02/2004; 12(1):93-102. · 3.09 Impact Factor
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  Available from: kobe-u.ac.jp

  Article: Variability of chromosomal DNA contents in maize (Zea mays L.) inbred and hybrid lines.

  Jai-Heon Lee, Kathiravetpilla Arumuganathan, Shawn M Kaeppler, Seong-Whan Park, Kee-Young Kim, Young-Soo Chung, Doh-Hoon Kim, Kiichi Fukui
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  ABSTRACT: The flow karyotypes of different maize (Zea mays L.) inbred and hybrid lines were analyzed. The accumulation and isolation of large quantities of high-quality metaphase chromosomes from root tips was achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synchronized maize root tips and analyzed by flow cytometry. Variations of experimental flow karyotypes were detected among inbred and hybrid lines in terms of the positions and/or the numbers of chromosome peaks. The 2C DNA amount among eight inbred lines ranged from 5.09 to 5.52 pg. The selection of appropriate maize lines is critical for sorting specific single chromosome types. At least five different chromosome types can be discriminated and sorted from five maize lines. The variability of DNA content in maize chromosome 1 was 9.1%, ranging from 0.685 to 0.747 pg. Differences were detected in the DNA content of homologous chromosome 1 of hybrid lines.
  Planta 09/2002; 215(4):666-71. · 3.00 Impact Factor