Graham L Collingridge

University of Bristol, Bristol, England, United Kingdom

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Publications (315)2460.3 Total impact

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    ABSTRACT: The roles of both kainate and metabotropic glutamate receptors in mossy fibre long-term potentiation (MF-LTP) have been extensively studied in hippocampal brain slices, but the findings are controversial. In the present study we have addressed the roles of both metabotropic glutamate receptors (mGluRs) and kainate receptors (KARs) in MF-LTP in anaesthetised rats. We found that MF-LTP could be induced in the presence of either GluK1-selective KAR antagonists or group I mGluR antagonists. However, LTP was inhibited when both of the group I mGluRs as well as GluK1-KARs were simultaneously inhibited. Either mGlu1 or mGlu5 receptor activation is sufficient to induce this form of LTP, since selective inhibition of either subtype alone, together with inhibition of KARs, did not inhibit MF-LTP. These data suggest that mGlu1 receptors, mGlu5 receptors and GluK1-containing receptors are all engaged during high frequency stimulation, but that the activation of any one of these receptors alone is sufficient for the induction of MF-LTP in vivo. This article is protected by copyright. All rights reserved. © 2015 Wiley Periodicals, Inc.
    Hippocampus 03/2015; DOI:10.1002/hipo.22460 · 4.30 Impact Factor
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    ABSTRACT: N-methyl-D-aspartate receptors (NMDARs) are known for their role in the induction of long-term potentiation (LTP). Here we start by reviewing the early evidence for their role in LTP at CA1 synapses in the hippocampus. We then discuss more recent evidence that NMDAR dependent synaptic plasticity at these synapses can be separated into mechanistically distinct components. An initial phase of the synaptic potentiation, which is generally termed short-term potentiation (STP), decays in an activity-dependent manner and comprises two components that differ in their kinetics and NMDAR subtype dependence. The faster component involves activation of GluN2A subunits whereas the slower component involves activation of GluN2B and GluN2D subunits. The stable phase of potentiation, commonly referred to as LTP, requires activation of primarily triheteromeric NMDARs containing both GluN2A and GluN2B subunits. In new work, we compare STP with a rebound potentiation (RP) that is induced by NMDA application and conclude that they are different phenomena. We also report that NMDAR dependent long-term depression (NMDAR-LTD) is sensitive to a glycine site NMDAR antagonist. We conclude that NMDARs are not synonymous for either LTP or memory. Whilst important for the induction of LTP at many synapses in the CNS, not all forms of LTP require the activation of NMDARs. Furthermore, NMDARs mediate the induction of other forms of synaptic plasticity and are important for synaptic transmission. It is, therefore, not possible to equate NMDARs with LTP though they are intimately linked. Copyright © 2015. Published by Elsevier B.V.
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    ABSTRACT: Chronic pain can lead to anxiety and anxiety can enhance the sensation of pain. Unfortunately, little is known about the synaptic mechanisms that mediate these re-enforcing interactions. Here we characterized two forms of long-term potentiation (LTP) in the anterior cingulate cortex (ACC); a presynaptic form (pre-LTP) that requires kainate receptors and a postsynaptic form (post-LTP) that requires N-methyl-D-aspartate receptors. Pre-LTP also involves adenylyl cyclase and protein kinase A and is expressed via a mechanism involving hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. Interestingly, chronic pain and anxiety both result in selective occlusion of pre-LTP. Significantly, microinjection of the HCN blocker ZD7288 into the ACC in vivo produces both anxiolytic and analgesic effects. Our results provide a mechanism by which two forms of LTP in the ACC may converge to mediate the interaction between anxiety and chronic pain. Copyright © 2015 Elsevier Inc. All rights reserved.
    Neuron 12/2014; 85(2). DOI:10.1016/j.neuron.2014.12.021 · 15.98 Impact Factor
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    ABSTRACT: Previous studies have shown that a family of phosphoinositide 3-kinases (PI3Ks) plays pivotal roles in the brain; in particular, we previously reported that knockout of the ¿ isoform of PI3K (PI3K¿) in mice impaired synaptic plasticity and reduced behavioral flexibility. To further examine the role of PI3K¿ in synaptic plasticity and hippocampus-dependent behavioral tasks we overexpressed p110¿, the catalytic subunit of PI3K¿, in the hippocampal CA1 region. We found that the overexpression of p110¿ impairs NMDA receptor-dependent long-term depression (LTD) and hippocampus-dependent spatial learning in the Morris water maze (MWM) task. In contrast, long-term potentiation (LTP) and contextual fear memory were not affected by p110¿ overexpression. These results, together with the previous knockout study, suggest that a critical level of PI3K¿ in the hippocampus is required for successful induction of LTD and normal learning.
    Molecular Brain 11/2014; 7(1):78. DOI:10.1186/s13041-014-0078-6 · 4.35 Impact Factor
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    ABSTRACT: Endoplasmic reticulum (ER) is motile within dendritic spines, but the mechanisms underlying its regulation are poorly understood. To address this issue, we have simultaneously imaged morphology and ER content of dendritic spines in cultured dissociated mouse hippocampal neurons. Over a 10 min period, spines were highly dynamic, with spines both increasing and decreasing in volume. ER was present in approximately 50% of spines and was also highly dynamic, with a net increase over this period of time. Inhibition of the endogenous activation of NMDA receptors resulted in a reduction in ER growth. Conversely, augmentation of the synaptic activation of NMDA receptors, by elimination of striatal-enriched protein tyrosine phosphatase (STEP), resulted in enhanced ER growth. Therefore, NMDA receptors rapidly regulate spine ER dynamics.
    Molecular Brain 08/2014; 7(1):60. DOI:10.1186/s13041-014-0060-3 · 4.35 Impact Factor
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  • Graham L Collingridge, Stephane Peineau
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    ABSTRACT: How do microglia regulate synaptic function? In this issue of Neuron, Zhang et al. (2014) describe a novel form of long-term depression of AMPA receptor-mediated synaptic transmission in the hippocampus involving the activation of microglia.
    Neuron 04/2014; 82(1):3-6. DOI:10.1016/j.neuron.2014.03.019 · 15.98 Impact Factor
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    ABSTRACT: The microtubule-associated protein tau is a principal component of neurofibrillary tangles, and has been identified as a key molecule in Alzheimer's disease and other tauopathies. However, it is unknown how a protein that is primarily located in axons is involved in a disease that is believed to have a synaptic origin. To investigate a possible synaptic function of tau, we studied synaptic plasticity in the hippocampus and found a selective deficit in long-term depression (LTD) in tau knockout mice in vivo and in vitro, an effect that was replicated by RNAi knockdown of tau in vitro. We found that the induction of LTD is associated with the glycogen synthase kinase-3-mediated phosphorylation of tau. These observations demonstrate that tau has a critical physiological function in LTD.
    Philosophical Transactions of The Royal Society B Biological Sciences 01/2014; 369(1633):20130144. DOI:10.1098/rstb.2013.0144 · 6.31 Impact Factor
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    ABSTRACT: N-methyl-d-aspartate receptor (NMDAR)-dependent long-term potentiation (LTP) is extensively studied since it is believed to use the same molecular mechanisms that are required for many forms of learning and memory. Unfortunately, many controversies exist, not least the seemingly simple issue concerning the locus of expression of LTP. Here, we review our recent work and some of the extensive literature on this topic and present new data that collectively suggest that LTP can be explained, during its first few hours, by the coexistence of at least three mechanistically distinct processes that are all triggered by the synaptic activation of NMDARs.
    Philosophical Transactions of The Royal Society B Biological Sciences 01/2014; 369(1633):20130131. DOI:10.1098/rstb.2013.0131 · 6.31 Impact Factor
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    T V P Bliss, G L Collingridge, R G M Morris
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    ABSTRACT: We summarize the reviews and research papers submitted by speakers at a discussion meeting on Synaptic Plasticity in Health and Disease held at the Royal Society, London on 2-3 December 2013, and a subsequent satellite meeting convened at the Royal Society/Kavli Centre at Chicheley Hall on 4-5 December 2013. Together, these contributions give an overview of current research and controversies in a vibrant branch of neuroscience with important implications for the understanding of many forms of learning and memory, and a wide spectrum of neurological and cognitive disorders.
    Philosophical Transactions of The Royal Society B Biological Sciences 01/2014; 369(1633):20130129. DOI:10.1098/rstb.2013.0129 · 6.31 Impact Factor
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    ABSTRACT: In this review, we focus on the role of the Shank family of proteins in autism. In recent years, autism research has been flourishing. With genetic, molecular, imaging and electrophysiological studies being supported by behavioural studies using animal models, there is real hope that we may soon understand the fundamental pathology of autism. There is also genuine potential to develop a molecular-level pharmacological treatment that may be able to deal with the most severe symptoms of autism, and clinical trials are already underway. The Shank family of proteins has been strongly implicated as a contributing factor in autism in certain individuals and sits at the core of the alleged autistic pathway. Here, we analyse studies that relate Shank to autism and discuss what light this sheds on the possible causes of autism.
    Philosophical Transactions of The Royal Society B Biological Sciences 01/2014; 369(1633):20130143. DOI:10.1098/rstb.2013.0143 · 6.31 Impact Factor
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    ABSTRACT: The neuroendocrine response to episodes of acute stress is crucial for survival whereas the prolonged response to chronic stress can be detrimental. Learning and memory are particularly susceptible to stress with cognitive deficits being well characterized consequences of chronic stress. Although there is good evidence that acute stress can enhance cognitive performance, the mechanism(s) for this are unclear. We find that hippocampal slices, either prepared from rats following 30 min restraint stress or directly exposed to glucocorticoids, exhibit an N-methyl-d-aspartic acid receptor-independent form of long-term potentiation. We demonstrate that the mechanism involves an NMDA receptor and PKA-dependent insertion of Ca(2+)-permeable AMPA receptors into synapses. These then trigger the additional NMDA receptor-independent form of LTP during high frequency stimulation.
    Brain 11/2013; DOI:10.1093/brain/awt293 · 10.23 Impact Factor
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    ABSTRACT: The insular cortex (IC) is known to play important roles in higher brain functions such as memory and pain. Activity-dependent long-term depression (LTD) is a major form of synaptic plasticity related to memory and chronic pain. Previous studies of LTD have mainly focused on the hippocampus, and no study in the IC has been reported. In this study, using a 64-channel recording system, we show for the first time that repetitive low-frequency stimulation (LFS) can elicit frequency-dependent LTD of glutamate receptor-mediated excitatory synaptic transmission in both superficial and deep layers of the IC of adult mice. The induction of LTD in the IC required activation of the N-methyl-d-aspartate (NMDA) receptor, metabotropic glutamate receptor (mGluR)5, and L-type voltage-gated calcium channel. Protein phosphatase 1/2A and endocannabinoid signaling are also critical for the induction of LTD. In contrast, inhibiting protein kinase C, protein kinase A, protein kinase Mζ or calcium/calmodulin-dependent protein kinase II did not affect LFS-evoked LTD in the IC. Bath application of the group I mGluR agonist (RS)-3,5-dihydroxyphenylglycine produced another form of LTD in the IC, which was NMDA receptor-independent and could not be occluded by LFS-induced LTD. Our studies have characterised the basic mechanisms of LTD in the IC at the network level, and suggest that two different forms of LTD may co-exist in the same population of IC synapses.
    European Journal of Neuroscience 08/2013; DOI:10.1111/ejn.12330 · 3.67 Impact Factor
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    ABSTRACT: Inhibition of Arp2/3-mediated actin polymerization by PICK1 is a central mechanism to AMPA receptor (AMPAR) internalization and long-term depression (LTD), although the signaling pathways that modulate this process in response to NMDA receptor (NMDAR) activation are unknown. Here, we define a function for the GTPase Arf1 in this process. We show that Arf1-GTP binds PICK1 to limit PICK1-mediated inhibition of Arp2/3 activity. Expression of mutant Arf1 that does not bind PICK1 leads to reduced surface levels of GluA2-containing AMPARs and smaller spines in hippocampal neurons, which occludes subsequent NMDA-induced AMPAR internalization and spine shrinkage. In organotypic slices, NMDAR-dependent LTD of AMPAR excitatory postsynaptic currents is abolished in neurons expressing mutant Arf1. Furthermore, NMDAR stimulation downregulates Arf1 activation and binding to PICK1 via the Arf-GAP GIT1. This study defines Arf1 as a critical regulator of actin dynamics and synaptic function via modulation of PICK1.
    Neuron 07/2013; 79(2):293-307. DOI:10.1016/j.neuron.2013.05.003 · 15.98 Impact Factor
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    Dataset: cdd201079a
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    ABSTRACT: The insular cortex (IC) is widely believed to be an important forebrain structure involved in cognitive and sensory processes such as memory and pain. However, little work has been performed at the cellular level to investigate the synaptic basis of IC-related brain functions. To bridge the gap, the present study was designed to characterize the basic synaptic mechanisms for insular long-term potentiation (LTP). Using a 64-channel recording system, we found that an enduring form of late-phase LTP (L-LTP) could be reliably recorded for at least 3 h in different layers of the IC slices following theta burst stimulation. The induction of insular LTP is protein synthesis-dependent and requires activation of both GluN2A and GluN2B subunits of the NMDA receptor, L-type voltage-gated calcium channels, and metabotropic glutamate receptor 1. The paired-pulse facilitation ratio was unaffected by insular L-LTP induction, and expression of insular L-LTP requires the recruitment of postsynaptic calcium-permeable AMPA receptors. Our results provide the first in vitro report of long-term multi-channel recordings of L-LTP in the IC from adult mice, and suggest its potential important roles in insular-related memory and chronic pain.
    Journal of Neurophysiology 05/2013; 110(2). DOI:10.1152/jn.01104.2012 · 3.04 Impact Factor
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    ABSTRACT: OBJECTIVE: The concept of inflammation-induced sensitization is emerging in the field of perinatal brain injury, stroke, Alzheimer disease, and multiple sclerosis. However, mechanisms underpinning this process remain unidentified. METHODS: We combined in vivo systemic lipopolysaccharide-induced or interleukin (IL)-1β-induced sensitization of neonatal and adult rodent cortical neurons to excitotoxic neurodegeneration with in vitro IL-1β sensitization of human and rodent neurons to excitotoxic neurodegeneration. Within these inflammation-induced sensitization models, we assessed metabotropic glutamate receptors (mGluR) signaling and regulation. RESULTS: We demonstrate for the first time that group I mGluRs mediate inflammation-induced sensitization to neuronal excitotoxicity in neonatal and adult neurons across species. Inflammation-induced G protein-coupled receptor kinase 2 (GRK2) downregulation and genetic deletion of GRK2 mimicked the sensitizing effect of inflammation on excitotoxic neurodegeneration. Thus, we identify GRK2 as a potential molecular link between inflammation and mGluR-mediated sensitization. INTERPRETATION: Collectively, our findings indicate that inflammation-induced sensitization is universal across species and ages and that group I mGluRs and GRK2 represent new avenues for neuroprotection in perinatal and adult neurological disorders.
    Annals of Neurology 05/2013; 73(5). DOI:10.1002/ana.23868 · 11.91 Impact Factor
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    ABSTRACT: Metabotropic glutamate (mGlu) receptors are implicated in many neurological and psychiatric diseases and are the targets of therapeutic agents currently in clinical development. Their activation has diverse effects in the central nervous system (CNS) that includes an involvement in synaptic plasticity. We previously reported that the brief exposure of hippocampal slices to dihydroxyphenylglycine (DHPG) can result in a long-term depression (LTD) of excitatory synaptic transmission. Surprisingly, this LTD could be fully reversed by mGlu receptor antagonists in a manner that was itself fully reversible upon washout of the antagonist. Here, 15 years after the discovery of DHPG-LTD and its reversible reversibility, we summarise these initial findings. We then present new data on DHPG-LTD, which demonstrates that evoked epileptiform activity triggered by activation of group I mGlu receptors can also be reversibly reversed by mGlu receptor antagonists. Furthermore, we show that the phenomenon of reversible reversibility is not specific to group I mGlu receptors. We report that activation of group II mGlu receptors in the temporo-ammonic pathway (TAP) and mossy fibre pathway within the hippocampus and in the cortical input to neurons of the lateral amygdala induces an LTD that is reversed by LY341495, a group II mGlu receptor antagonist. We also show that activation of group III mGlu8 receptors induces an LTD at lateral perforant path inputs to the dentate gyrus and that this LTD is reversed by MDCPG, an mGlu8 receptor antagonist. In conclusion, we have shown that activation of representative members of each of the three groups of mGlu receptors can induce forms of LTD than can be reversed by antagonists, and that in each case washout of the antagonist is associated with the re-establishment of the LTD.
    Neuropharmacology 03/2013; 74. DOI:10.1016/j.neuropharm.2013.03.011 · 4.82 Impact Factor
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    ABSTRACT: Postsynaptic Ca2+ transients triggered by neurotransmission at excitatory synapses are a key signaling step for the induction of synaptic plasticity and are typically recorded in tissue slices using two-photon fluorescence imaging with Ca2+-sensitive dyes. The signals generated are small with very low peak signal/noise ratios (pSNRs) that make detailed analysis problematic. Here, we implement a wavelet-based de-noising algorithm (PURE-LET) to enhance signal/noise ratio for Ca2+ fluorescence transients evoked by single synaptic events under physiological conditions. Using simulated Ca2+ transients with defined noise levels, we analyzed the ability of the PURE-LET algorithm to retrieve the underlying signal. Fitting single Ca2+ transients with an exponential rise and decay model revealed a distortion of trise but improved accuracy and reliability of tdecay and peak amplitude after PURE-LET de-noising compared to raw signals. The PURE-LET de-noising algorithm also provided a ~30-dB gain in pSNR compared to ~16-dB pSNR gain after an optimized binomial filter. The higher pSNR provided by PURE-LET de-noising increased discrimination accuracy between successes and failures of synaptic transmission as measured by the occurrence of synaptic Ca2+ transients by ~20% relative to an optimized binomial filter. Furthermore, in comparison to binomial filter, no optimization of PURE-LET de-noising was required for reducing arbitrary bias. In conclusion, the de-noising of fluorescent Ca2+ transients using PURE-LET enhances detection and characterization of Ca2+ responses at central excitatory synapses.
    Biophysical Journal 03/2013; 104(5):1006. DOI:10.1016/j.bpj.2013.01.015 · 3.83 Impact Factor
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    Arturas Volianskis, Graham L Collingridge, Morten S Jensen
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    ABSTRACT: Long-term potentiation (LTP), a cellular model of learning and memory, is generally regarded as a unitary phenomenon that alters the strength of synaptic transmission by increasing the postsynaptic response to the release of a quantum of neurotransmitter. LTP, at CA3-CA1 synapses in the hippocampus, contains a stimulation-labile phase of short-term potentiation (STP, or transient LTP, t-LTP) that decays into stable LTP. By studying the responses of populations of neurons to brief bursts of high-frequency afferent stimulation before and after the induction of LTP, we found that synaptic responses during bursts are potentiated equally during LTP but not during STP. We show that STP modulates the frequency response of synaptic transmission whereas LTP preserves the fidelity. Thus, STP and LTP have different functional consequences for the transfer of synaptic information.
    02/2013; 1:e3. DOI:10.7717/peerj.3

Publication Stats

31k Citations
2,460.30 Total Impact Points


  • 1985–2015
    • University of Bristol
      • • Centre for Synaptic Plasticity
      • • School of Physiology and Pharmacology
      • • Medical School
      Bristol, England, United Kingdom
  • 2010–2014
    • Seoul National University
      • Brain and Cognitive Sciences
      Sŏul, Seoul, South Korea
  • 2013
    • Paris Diderot University
      Lutetia Parisorum, Île-de-France, France
  • 2008
    • The American University of Rome
      Roma, Latium, Italy
  • 2005
    • University of Helsinki
      • Department of Biological and Environmental Sciences
      Helsinki, Province of Southern Finland, Finland
  • 1996
    • The University of Edinburgh
      • Credit Research Centre
      Edinburgh, Scotland, United Kingdom
  • 1991–1996
    • University of Birmingham
      Birmingham, England, United Kingdom
  • 1989
    • Hochschule Magdeburg
      Magdeburg, Saxony-Anhalt, Germany
  • 1983–1984
    • University of British Columbia - Vancouver
      Vancouver, British Columbia, Canada
  • 1981–1982
    • The School of Pharmacy
      • Pharmacology
      Londinium, England, United Kingdom
  • 1979–1981
    • University of London
      • The School of Pharmacy
      London, ENG, United Kingdom
    • Julphar School of Pharmacy
      Брауншвейг, Ohio, United States