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ABSTRACT: PURPOSE. TO INVESTIGATE THE PROTECTIVE EFFECT OF PIOGLITAZONE ON THE RAT RETINA AFTER ISCHEMIA/REPERFUSION (I/R) INJURY AND TO EXPLORE ITS POSSIBLE MECHANISMS. METHODS. RETINAL ISCHEMIA WAS INDUCED BY INCREASING THE INTRAOCULAR PRESSURE TO 110MMHG FOR 60MINUTES, AND PIOGLITAZONE WAS DELIVERED 3 HOURS BEFORE THE I/R. RETINAL DAMAGE WAS QUANTIFIED BY MEASURING THE THICKNESS OF THE RETINA, THE FUNCTIONAL CHANGES OF VISUAL EVOKED POTENTIAL (VEP) AND ELECTRORETINOGRAPHY (ERG), AND THE RETINAL GANGLION CELLS (RGCS) NUMBER AT 7 DAYS AFTER I/R INJURY. REAL-TIME PCR AND WESTERN BLOT ANALYSIS WERE PERFORMED TO MEASURE THE GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP) EXPRESSION. RETINAL CELL APOPTOSIS WAS DETECTED BY TUNEL ASSAY AT 24 HOURS AFTER REPERFUSION. NUCLEAR FACTOR-KAPPAB (NF-B), BAX, AND BCL-2 IN THE RETINA WERE DETERMINED BY WESTERN BLOT ANALYSIS. RESULTS. THE I/R PRODUCED DEGENERATIVE EFFECTS PRIMARILY IN THE GANGLION CELL LAYER, INNER PLEXIFORM LAYER AND INNER NUCLEAR LAYER. PIOGLITAZONE MAINTAINED THE RETINAL THICKNESS, PROMOTED THE SURVIVAL OF RETINAL GANGLION CELLS, AND ATTENUATED THE DESTRUCTION OF ERG AND VEP CAUSED BY THE I/R. PIOGLITAZONE PRETREATMENT ALSO SUPPRESSED NF-B ACTIVATION AND ALTERED THE GFAP OVEREXPRESSION. THE NUMBER OF TUNEL LABELED CELLS SIGNIFICANTLY REDUCED IN THE RETINAS PRETREATED WITH PIOGLITAZONE, AND THE BAX: Bcl-2 ratio was much lower in the retinas pretreated with pioglitazone than that in the I/R group. CONCLUSIONS. Pioglitazone could inhibit the activation of the glia cells, prevent cell apoptosis, and protect the retina from subsequent cellular damage caused by the retinal I/R. The possible mechanism might involve the NF-κB pathway.
Investigative ophthalmology & visual science 04/2013; · 3.43 Impact Factor
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Liu-mei Hu,
Xia Lei,
Bo Ma,
Yu Zhang,
Yan Yan,
Ya-lan Wu,
Ge-zhi Xu, Wen Ye,
Ling Wang,
Guo-xu Xu,
Guo-tong Xu,
Li Wei-Ye
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ABSTRACT: To investigate the possible involvement of erythr opoietin (EPO)/erythropoietin receptor (EPOR) system in neovascularization and vascular regeneration in diabetic retinopathy (DR).
EPOR positive circulating progenitor cells (CPCs: CD34(+)) and endothelial progenitor cells (EPCs: CD34(+)KDR(+)) were assessed by flow cytometry in type 2 diabetic patients with different stages of DR. The cohort consisted of age- and sex-matched control patients with out diabetes ( n=7),non-proliferative DR (NPDR, n=7),non-proliferative DR (PDR, n=8), and PDR complicated with diabetic nephr opathy (PDR-DN, n=7).
The numbers of EPOR(+) CPCs and EPOR(+) EPCs were reduced remarkably in NPDR compared with the control group (both Pü0.01), whereas rebounded in PDR and PDR-DN groups in varyingdegrees. Similar changes were observed in respect of the proportion of EPOR(+)CPCs in CPCs (NPDR vs. control, Pü0.01) and that of EPOR(+) EPCs in EPCs (NPDR vs. control, Pü0.05).
Exogenous EPO, mediated via the EPO/EPOR system of EPCs, may alleviate the impaired vascular regeneration in NPDR, whereas it might aggravate retinal neovascularization in PDR due to a rebound of EPOR(+)EPCs associated with ischemia.
Chinese Medical Sciences Journal 06/2011; 26(2):69-76.
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ABSTRACT: To evaluate the protective effect of intravitreal injection of exendin-4 analogue (E4a) in early diabetic retinopathy (DR) and to explore its possible mechanism.
Forty Sprague-Dawley rats were divided into three groups: normal (N), diabetic (D), and E4a-treated diabetic rats (E4a). Diabetes was induced by streptozotocin. Rats in the E4a group were treated with E4a (0.1 μg/2μL/eye), whereas the N and D groups were treated with the equivalent volume of normal saline. Electroretinography was performed at 1 month and 3 months after diabetes onset. Thicknesses and cell counts in each layer of the retina were evaluated. The concentration of glutamate was measured by high-performance liquid chromatography (HPLC). Expressions of glucagon-like peptide-1 receptor (GLP-1R) and GLAST (excitatory amino acid transporter) were detected at mRNA and protein levels and verified by immunohistochemistry in vitro and in vivo. The rMc-1 cells were cultured under high-glucose medium (25 mM), which mimicked diabetic conditions. Effects of E4a (10 μg/mL) were also tested in the rMc-1 culture system.
E4a prevented the reduction in b-wave amplitude and oscillatory potential amplitude caused by diabetes. It also prevented the cell loss of outer nuclear layer and inner nuclear layer; the thickness and cell count in the outer nuclear layer were decreased in 1-month diabetic rats. The concentration of glutamate in the retina was higher in diabetic rats and was significantly reduced in the E4a-treated group. Consistent with such changes, retinal GLP-1R and GLAST expression were reduced in the diabetic retina but upregulated in E4a-treated rats. No improvement was found in the retina in both functional and morphologic parameters 3 months after treatment.
Intravitreal administration of E4a can prevent the retina, functionally and morphologically, from the insults of diabetes in rats. GLP-1R and GLAST were proved to exist in the rat retina, and their lowered expressions in the diabetic retina might be related to retinal damage by increasing the retinal glutamate. E4a might protect the retina by reducing the glutamate level through upregulating GLP-1R and GLAST, as observed in retinal Müller cells in this study, but this protective effect was transient. Thus, this could be a potential approach for the treatment of DR.
Investigative ophthalmology & visual science 01/2011; 52(1):278-85. · 3.43 Impact Factor
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ABSTRACT: To evaluate the visual field protective effect of Erigeron breviscapus (vant.) Hand. Mazz. (EBHM) extract on glaucoma with controlled intraocular pressure (IOP).
Forty patients (40 eyes) with primary open-angle glaucoma, visual field defects and a postsurgical IOP of <18 mmHg were enrolled. The EBHM and placebo tablets were given orally according to the randomized and double-blind principle. Two tablets (of either EBHM or placebo) were taken three times a day for a period of 6 months. Patients were examined every 2 months after treatment commenced. At the end of the study, the results were given to the drug manufacturer.
All patients completed the prospective, randomized, double-blind, clinical trial. No obvious adverse effects were found in patients during the treatment period. In the placebo group, no significant difference was found in mean defect (MD) or mean sensitivity (MS) between the values at pre-treatment and after 2, 4, and 6 months of treatment. After 6 months of EBHM treatment, the MD was significantly decreased and the MS was significantly increased compared with pre-treatment (p < 0.05). In the patients with moderate and late glaucoma, the MD was significantly decreased and the MS was significantly increased after 2, 4, and 6 months of EBHM treatment compared with pre-treatment.
EBHM extract may have a partial protective effect on the visual field of glaucoma patients with controlled IOP. Further studies are needed to determine the safety and effectiveness of long-term EBHM treatment.
Drugs in R&D. 01/2010; 10(2):75-82.
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ABSTRACT: To compare static visual field perimetry with Octopus 101 and Goldmann perimetry in patients with pituitary adenomas; To identify the factors of visual field defects and visual field prognosis.
Retrospective nested case-control study. Visual acuity, fundus, computed tomography (CT) or magnetic resonance imaging (MRI) and visual field were pre-operated assessed in 169 patients (338 eyes) diagnosed pituitary adenoma at the Department of Neurosurgery in Huashan Hospital between Feb. 2006 and Feb. 2007. 334 eyes were underwent Goldmann perimetry, while 323 eyes were underwent static visual field perimetry with Octopus 101 perimeter. In the 169 patients, 28 patients (56 eyes) were followed up post-operatively for 3 to 6 months. Statistical analysis was performed using SPSS 15.0 software. T test and rank sum test were used for continuous variables, and chi-square test and Fisher's exact test were used for categorical variables. We also carry out Logistic regression.
Of the 169 patients, unilateral or bilateral visual field defects were observed in 133 cases. In these 323 eyes, 231 eyes showed visual field defects with static visual field perimetry, while 196 eyes in 334 eyes showed defects with Goldmann perimetry. Earlier age of onset, larger tumors and poor preoperative corrected visual acuity were more frequent in patients with visual field defects than in patients with normal visual fields (t = 4.802, 7.930; chi(2) = 28.210, P < 0.01). In the 28 followed-up patients (55 eyes), visual acuity was improved in 69.05% eyes and visual field was improved in 85.71% eyes after operation. There were significant differences in age of onset and preoperative visual field defects in upper temporal quadrants between patients with visual field returned to normal after the operation and patients with visual field improvement only (t = 2.525, P = 0.023; chi(2) = 6.218, P = 0.013).
Static visual field perimetry with Octopus 101 perimeter is more sensitive than Goldmann perimetry in the early diagnosis of pituitary adenomas. Patients with visual field abnormalities are significantly with earlier onset age, larger tumors and worse preoperative visual acuity than patients free of visual field defects. The onset age and the degree of impairment in the preoperative visual field in the upper temporal quadrant may be significant factors for visual field prognosis.
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology 12/2009; 45(12):1074-9.
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ABSTRACT: Diabetic retinopathy (DR) is one of the leading causes of blindness, affecting over 90% of diabetics. Exendin-4 (E4) is a potent and long-acting agonist of the glucagon-like peptide-1 (GLP-1) receptor. GLP-1 is an insulinotropic gut peptide, which normalizes blood glucose level and is now being tested in clinical trials as a treatment for diabetes. The purpose of our study was to explore the protective effect of subcutaneous (sc.) exendin-4 analogue (E4a) on early DR.
Expression of GLP-1R was detected at both mRNA and protein levels and verified by immunohistochemistry. Thirty-six Sprague-Dawley (SD) rats were included in the experiment. Diabetes was induced by intraperitoneal injection (ip) of streptozotocin (STZ). The rats were divided into three groups: normal control (N), diabetic control (D) and E4a-treated diabetic (E4a) group. For the E4a group, the rats were treated with E4a (sc.0.05 microg/g BW/day); for the N and D groups, the rats were treated with normal saline (NS, sc). Blood glucose levels and body weight were measured weekly. Electroretinogram (ERG) was performed 1 and 3 months after diabetes onset. The retinal thickness and cell counts in each layer were evaluated under light microscopy after ERG examination.
GLP-1R was expressed at both mRNA and protein levels in the retina of SD rats. Immunostaining of the rat retina revealed that GLP-1R was predominantly expressed in the inner layer of the retina. E4a can reduce the blood glucose level of diabetic rats to the normal control level. B-wave amplitudes and OPs decreased with the progress of diabetes, and E4a prevents the loss of b-wave amplitude and OPs caused by diabetes. The retinal thickness was reduced in a diabetes-duration-dependent fashion. The cell counts of both ONL and INL were reduced accordingly in the diabetic rats. E4a prevented cell loss and maintained a normal thickness.
GLP-1R is expressed in rat retina. Apoptosis is an important constituent of retinal cell death in early DR. E4a administration can reverse the changes of ERG, prevent the retinal cell death and maintain normal retinal thickness in diabetic rats. Therefore, this is a potent approach for treatment of early DR.
Albrecht von Graæes Archiv für Ophthalmologie 01/2009; 247(5):699-706. · 2.17 Impact Factor
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Jing-fa Zhang,
Ya-lan Wu,
Jing-ying Xu, Wen Ye,
Yu Zhang,
Huan Weng,
Wo-dong Shi,
Guo-xu Xu,
Luo Lu,
Wei Dai,
Stephen H Sinclair,
Wei-ye Li,
Guo-tong Xu
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ABSTRACT: To study the pharmacokinetics and toxicity of intravitreal erythropoietin (EPO) for potential clinical use.
For toxicity study, 4 groups (60 rabbits) with intravitreal injection (IVit) of EPO were studied (10 U, 100 U, or 1,000 U) per eye for single injection and 0.6 U/eye (the designed therapeutic level in rabbits) for monthly injections (6X). Eye examination, flash electroretinogram (ERG), and fluorescein angiography (FA) were carried out before and after injection. The rabbits were killed for histological study at different intervals. For the pharmacokinetic study, after IVit of 5 U EPO into left eyes, 44 rabbits were killed at different intervals, and the EPO levels in vitreous, aqueous, retina and serum were analyzed by enzyme-linked immunosorbent assay.
At all of the time points examined, the eyes were within normal limits. No significant ERG or FA change was observed. The histology of retina remained unchanged. The pharmacokinetic profile of EPO in ocular compartments was summarized as follows. The half-life times of EPO in vitreous, aqueous and serum were 2.84, 3.24 and 2.12 d, respectively; and Cmax were 4615.75, 294.31 and 1.60 U/L, respectively. EPO concentrations in the retina of the injected eye peaked at 1.36 U/g protein at 6 h following injection, with the half-life observed to be 3.42 d.
IVit of EPO in a wide range is well tolerated and safe for rabbit eyes. At doses up to 10-fold higher than therapeutic levels, EPO has a pharmacokinetic profile with faster clearance, which is favorable for episodic IVit.
Acta Pharmacologica Sinica 12/2008; 29(11):1383-90. · 1.95 Impact Factor
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ABSTRACT: To explore the inhibitive effect of SB-431542 (an ALK5 inhibitor) on scar formation after glaucoma surgery and to identify the potential pharmacologic target(s).
Twenty-four New Zealand rabbits underwent filtration surgery on the right eye and were divided into a control group and three experimental groups (n=6). Human Tenon's fibroblast monolayer was scraped to generate a single gap, and then the control medium with SB-431542 only or containing 10 microg/L TGF-beta1 and SB-431542 (1-20 microM) was added. The cells were pretreated with SB-431542 or in control medium for 30 minutes before induction with 10 microg/L TGF-beta1 or 1 microg/L TGF-beta2. The expression of alpha-SM-actin, CTGF, and Col I, as well as changes in the Smad, ERK, P38, and AKT signaling pathways were detected.
In comparison with the control rabbits, the IOPs in the experimental groups remained at lower levels until day 25 (P<0.05) after the surgery. Histologic profiles showed that there was only a mild deposition of collagen in the subconjunctival space in the experimental groups. The cell growth and migration were inhibited effectively by SB-431542, regardless of whether TGF-beta was present in the culture system. SB-431542 abrogated TGF-beta-induced upregulation of alpha-SM-actin, CTGF, and Col I. It effectively inhibited the phosphorylation of Smad2 stimulated by TGF-beta but not that of the components of the MAPK pathways.
SB-431542 inhibits scar formation after glaucoma filtration surgery. The mechanism may be that SB-431542 interferes in the phosphorylation of Smad2, thus abrogating TGF-beta-induced fibroblast transdifferentiation and then decreasing Col I synthesis.
Investigative ophthalmology & visual science 12/2008; 50(4):1698-706. · 3.43 Impact Factor
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ABSTRACT: Non-obese diabetic (NOD) mice are a commonly used murine model for the study of Sjögren's syndrome. However, variations in susceptibility to the disease among the mice has often yielded less stable results. Based on the correlation between the pathological changes and the tear tests, we attempt to establish a simple screening procedure to assure the validity of experimental results by excluding those mice with poor susceptibility to dry eyes.
Seventy male NOD mice were recruited. The tear film break-up test (BUT) and the phenol red cotton thread test (CTT) were implemented while the mice were under anesthesia. The mice were divided into four groups (grades 1 to 4) based on their BUT readings, and four similar groups based on CTT measurements. Tear samples in each grade were collected for IL-1beta detection with ELISA. The lacrimal glands and conjunctiva of the mice were used to detect the levels of leucocyte common antigen (LCA). LCA-Positive staining was considered as the "gold standard" in the receiver operating characteristic curve (ROC curve) analysis. C57BL/6 mice were used as wild-type controls.
There were 13 (18.57%), 43 (61.43%), 10 (14.29%) and 4 (5.71%) mice in grades 1, 2, 3 and 4 by BUT test, and 34 (48.57%), 15 (21.43%), 14 (20.00%) and 7 (10.00%) in grades 1, 2, 3 and 4 by CTT test respectively. Fifty-one out of the 70 mice (72.86%) were detected LCA-positive, and they were mainly in grades 1 and 2 of both the BUT and CCT grading systems. ELISA showed significant variances of IL-beta levels among the four groups (p < 0.01), with much lower IL-beta levels in group 3 and 4 when both BUT and CTT were used for grouping. The tear IL-beta level in the wild-type mice was similar to those of the grade 4 mice, using either BUT or CTT for grouping. The ROC curve analysis provided optimal cutting lines, which were 2 seconds in BUT readings and 4 mm/min in CTT measurements respectively.
BUT and CTT tests are useful methods in screening high susceptible NOD mice. Cutting lines at BUT < or = 2 seconds and CTT < or = 4 mm/min provide a good balance between the assurance of susceptibility and the maximization of use of NOD mice for the study of Sjögren's syndrome.
Albrecht von Graæes Archiv für Ophthalmologie 09/2008; 247(1):59-66. · 2.17 Impact Factor
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ABSTRACT: To study the interaction between latanoprost and pilocarpine on cultured rabbit ciliary muscle (RCM) cells, and investigate the time courses of the two drugs, when given alone or in combination.
Cultured RCM cells were treated for 24 h with different concentrations of latanoprost acid, pilocarpine and mixtures of latanoprost acid and pilocarpine. RNA was extracted, expressions of matrix metalloproteinase 1 (MMP-1), tissue inhibitor of metalloproteinase 1 (TIMP-1) and TIMP-2 were detected by reverse transcription-polymerase chain reaction (RT-PCR), and the optimum concentrations of those drugs were found. Then the cells were treated with the optimum concentrations of those drugs for various periods. RNA was extracted after the treatment and expressions of MMP-1, TIMP-1 and TIMP-2 were detected by RT-PCR again. Changes in [Ca(2+)](i) were estimated by fluorescence measurement using the Ca(2+) indicator Fluo-3 AM with a laser scanning confocal microscope. [Ca(2+)](i) of each cell was monitored continually after administration of the drugs. Gray values at 5 s and 2, 4, 6, 8 and 10 min were chosen for statistical analysis, and the influence and time-effect relationship of those drugs on [Ca(2+)](i) of the cultured cells were evaluated.
Exposure of the cells to increasing concentrations of latanoprost acid induced increased MMP-1 mRNA and decreased TIMP-1 and TIMP-2 mRNA in a dose-dependent manner. After 24 h of treatment, the optimum concentration of latanoprost acid for maximal changes in MMP-1 and TIMP-2 expression was 2 x 10(-7)M, and for maximal changes in TIMP-1 expression, the optimum concentration was 5 x 10(-7)M. When the optimum concentrations of latanoprost acid were chosen to treat the cells for various periods, the optimum time of the peak MMP-1 expression and trough TIMP-1 expression was 24 h, and of the trough TIMP-2 expression, it was 36 h after initiation of treatment. No significant expression changes of MMP-1, TIMP-1 and TIMP-2 mRNA were found when the cells were treated with pilocarpine at any concentration or at any time. Exposure of the cells to the mixtures of latanoprost acid and pilocarpine induced the same changes and time course of MMP-1, TIMP-1, and TIMP-2 mRNA expression as exposure of the cells to latanoprost acid alone. Exposure of ciliary muscle cells to pilocarpine induced an increase in [Ca(2+)](i), with the peak of increase observed at 5 s after initiation of treatment; then [Ca(2+)](i) gradually decreased near to baseline level within 10 min. Exposure of the cells to latanoprost acid did not significantly change [Ca(2+)](i). Exposure of the cells to the mixtures of latanoprost acid and pilocarpine induced the same [Ca(2+)](i) change as exposure to pilocarpine alone.
Latanoprost and pilocarpine have no interaction in their various effects on the cultured RCM cells.
Ophthalmic Research 02/2007; 39(4):232-40. · 1.56 Impact Factor
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ABSTRACT: To study the situation for the follow eye visual field defect of patients with severe visual field loss in 1 eye from chronic glaucoma,and to analyze the relative risk factors for visual field defect in such eyes.
The visual field defect scores of fellow eyes were calculated in 47 cases [primary chronic angle-closed glaucoma (PACG) 23 cases and primary open angle glaucoma (POAG) 24 cases] with severe visual field loss in 1 eye (visual field defect score > or = 12). The relations were analyzed between the visual field defect scores of fellow eyes and the visual acuity,the maximal and the mean intraocular pressure (IOP), ages, refraction, diagnosis, the last periods of glaucoma, gender and the anti-glaucoma surgery history. Spearman correlation analyse was used to estimated the correlation between the visual field defect scores of fellow eyes and the above factors.
In the fellow eyes of the 23 cases PACG, 4 cases showed no visual field defect (score 0),5 cases showed the mild visual field defect (score 1-5), 7 cases showed the moderate visual field defect (score 6-11), 7 cases showed the severe or end-stage visual field defect (score 12-20). In the fellow eyes of the 24 cases POAG, 4 cases showed the mild visual field defect (score 1-5), 9 cases showed the moderate visual field defect (score 6-11), 11 cases showed the severe or end-stage visual field defect (score 12-20). The visual field defect score of fellow eyes in the last periods more than or equal to 10 years PACG group was significantly larger than that in the last periods less than 10 years PACG group (Z=2.8380, P=0.0224). The visual field defect score of fellow eyes in the mean IOP more than 18 mmHg PACG group was significantly larger than that in the mean IOP less than or equal to 18 mmHg PACG group (Z=1.9174, P=0.0276). The visual field defect score of fellow eyes in the anti-glaucoma surgery history POAG group was significantly larger than that in the non-anti-glaucoma surgery history POAG group (Z= 2.2377, P=0.0252). The significant positive correlation was found between the visual field defect scores and the mean IOP of the fellow eyes in the PACG group (r=0.4763, P= 0.0216),and the significant negative correlation was found between the visual field defect score and the visual acuity of the fellow eyes in the PACG group (r=-0.4416, P= 0.0349).
Among the PACG with severe visual field loss in 1 eye, the visual field defect extent in the fellow eyes was related to the mean IOP, the last periods of PACG and the visual acuity of the fellow eyes. The visual field defect score was positively correlated with the mean IOP, and negatively correlated with the visual acuity of the fellow eyes.
Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu 01/2005; 20(4):213-8.
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ABSTRACT: To evaluate the validity and reliability of visual field defect scoring method in glaucoma.
The visual field defects of 91 glaucoma cases (91 eyes) were scored with the Advanced Glaucoma Intervention Study (AGIS) modified scoring method, and analyzed the relation and correlation between the score and cup/disk ratio, mean defect (MD) and loss variance (LV).
The larger the score was, the larger the cup/disk ratio, MD and LV were. The significant positive correlation was found between the score and the cup/disk ratio (r = 0.8712), and the correlation coefficient was larger than that of between the MD, LV and cup/disk ratio.
The visual field defect score could more accurately reflect the situation of glaucomatous optic nerve damage than visual field indices, and could quantify the visual field damage extent in glaucoma.
Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu 01/2004; 19(4):218-20.
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ABSTRACT: To compare the threshold variability of blue-on-yellow (B/Y) perimetry with white-white(W/W) perimetry, and evaluate the reproducibility of B/Y perimetry.
The B/Y perimetry and W/W perimetry in the Octopus 101 perimetry were used to examine the visual fields of 12 normal subjects (24 eyes), 16 cases (32 eyes) of primary open angle glaucoma (POAG), and 7 cases (14 eyes) of suspected POAG respectively. The B/Y perimetry and W/W perimetry were repeated to examine within 2 weeks. The point by point threshold variability of the two perimetries were compared and analysed.
The total mean threshold variability for B/Y perimetry (2.61 +/- 0.94) dB was greater than that for W/W perimetry (2.11 +/- 0.90) dB in all subjects, but it had no significance (P = 0.6244). The total mean threshold variability for B/Y perimetry (2.07 +/- 0.54) dB was significantly greater than that for W/W perimetry (1.50 +/- 0.34) dB in normal subjects (P = 0.0006), while no significance was found in suspected POAG and POAG groups between the two perimetries (P = 0.0523 and 0.9371). The threshold variability in the areas of some eccentricities for B/Y perimetry were significantly greater than that for W/W perimetry in normal subjects and suspected POAG group, but no significance was found in POAG group for all eccentricities between the two perimetries.
The threshold variability for B/Y perimetry was greater than that for W/W perimetry in normal subjects, but no significance was found in suspected POAG and POAG between the two perimetries.
Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu 07/2002; 18(2):87-91.
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ABSTRACT: To evaluate the regression relevant reasons of laser in situ kertomileusis (LASIK) for treatment of myopia.
Four hundred and eight eyes of 250 patients with myopia who received LASIK were studied. They were divided into 2 groups according to preoperative diopters (-6.25- -10.00 D in 194 eyes; -10.25 -15.00 D in 214 eyes). The mean follow-up was 12 months, and the results of the postoperative visual acuity, refractive diopter, corneal thickness and the diameter of the ablation zone were statistically analyzed.
(1) A group: There were 173 normal operative eyes (89.2%, post-operative diopter < -1.00 D), the mean pre-operative corneal thickness was (549.5 +/- 31.5) microm, the mean intra-operative laser ablation diameter was (4.96 +/- 0.35) mm, and the post-operative refractive diopter was +0.50- -0.75 D. The regressive operative eyes: There were 21 eyes (10.8%, post- operative diopter >/= -1.00D), the mean preoperative corneal thickness was (547.7 +/- 37.0) microm (P > 0.05 in comparison with that of the normal operative eyes), the mean intra-operative laser ablation diameter was (4.64 +/- 0.41 ) mm (P < 0.01 in comparison with that of the normal operative eyes), and the mean post-operative diopter was (-1.33 +/- 0.58)D. (2) B group: There were 136 normal operative eyes (63.5%), the mean pre-operative corneal thickness was (560.9 +/- 30.9) microm, the mean intra-operative laser ablation diameter was (4.51 +/- 0.28) mm, and the post-operative diopter was +0.50- -0.75 D. The regressive operative eyes: There were 78 eyes (36.5%), the mean pre-operative corneal thickness was (538.0 +/- 31.0) microm (P < 0.01 in comparison with that of the normal operative eyes), the mean intra-operative laser ablation diameter was (4.22 +/- 0.34) mm (P < 0.01 in comparison with that of the normal operative eyes), and the mean post-operative diopter was (-1.99 +/- 1.01) D.
In cases with small laser ablation diameter and the thin pre-operative corneal thickness of high myopia, after the surgery refractive regression is easy to occur. Some modification of the surgical algorithms and laser nomogram will help to improve predictability and reduce regression.
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology 06/2002; 38(6):363-6.
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ABSTRACT: To evaluate the efficacy of non-perforating deep sclerectomy (NPDS) with amniotic membrane implantation for primary open-angle glaucoma (POAG).
Fourteen cases (23 eyes) of POAG underwent NPDS with amniotic membrane implantation. NPDS was performed with a fornix-based conjunctival flap using 0. 04% mitomycin C(MMC) under the scleral flap for 4 minutes. A 6 mm x 9 mm amniotic membrane was then placed under the scleral flap and sutured using 10-0 nylon.
One month postoperatively, the intraocular pressure (IOP) was less or equal to 21 mmHg in 23 eyes, with a total success rate of 100%. After 3 months of follow-up for 20 eyes, the completely success rate was 90%, and the partly success rate was 100%. After 6 months of follow-up for 11 eyes, the completely success rate was 72.7%, the partly success rate was 100%, and the visual acuity had no significance between the pre-operation and post-operation. After 12 months of follow-up for 6 eyes, the success rate was 66. 7%. A good bleb formation was seen in the eyes and the IOP was controlled successfully. No serious complication was found in all eyes.
Amniotic membrane is an effective and safe implantation material for NPDS.
Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu 06/2002; 18(2):76-9.
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ABSTRACT: To evaluate the effectiveness, predictability, and safety of laser in situ keratomileusis (LASIK) for correction of hyperopia and hyperopic astigmatism.
Fifty-four hyperopic eyes of 35 patients with a spherical equivalent refraction between +1.00 and +6.00 D were followed for at least 12 months following LASIK. All surgery was performed with the scanning Chiron Technolas Keracor 117C excimer laser. Data on uncorrected and spectacle-corrected visual acuity, predictability, stability of refraction, and complications were analyzed.
At 12 months, the average residual refraction was +0.29 +/- 0.78 D; 83.3% of eyes (45 eyes) were in the range of +/- 1.00 D and 61.1% of eyes (33 eyes) were within +/- 0.50 D of emmetropia. Fifty eyes (92.6%) had uncorrected visual acuity of 20/40 or better and 34 (63.0%) eyes had 20/20 or better. One eye (1.9%) lost two lines of best spectacle-corrected visual acuity and two eyes (3.7%) gained two or more lines. Two patients (two eyes, 3.7%) had complaints of halos and one patient (one eye, 1.9%) had glare at 12 months after LASIK for hyperopia.
LASIK was used to treat hyperopia from +1.00 to +6.00 D with good predictability and safety. Primary and second hyperopia require different nomograms, according to our experience.
Journal of refractive surgery (Thorofare, N.J.: 1995) 18(4):435-8. · 2.54 Impact Factor
