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ABSTRACT: The purpose of this study was to establish a palatal organ culture method and to investigate the palatogenesis in vitro.
20 pregnant 14-day mice were killed, embryos were separated ascetically, and palatal shelves were dissected and placed on a modified Trowell's system. All explants were cultured 24 h and 48 h respectively. Finally, all explants were embedded and stained by Hematoxylin and Eosin.
All explants grew healthy. After incubation for 24 h, medial edge epithelium maintained, whereas after 48 h, medial edge epithelium disappeared, bilateral mesenchymal cells contacted, palates fused.
This method provides an effective way for investigating the etiology of cleft palate in vitro.
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 08/2011; 29(4):413-4.
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ABSTRACT: The authors describe a patient with bilateral Tessier no. 6 and no. 7 clefts. The case consists of macrostomia combined with maxillary, zygomatico-orbito-temporal deformities, dental anomalies and mandibular retrusion. In addition, the cleft is located bilaterally in the maxillary arch with a double deciduous posterior dentition. This is rare. The clinical and radiological features are reported and the treatment plans are discussed.
Journal of cranio-maxillo-facial surgery: official publication of the European Association for Cranio-Maxillo-Facial Surgery 11/2010; 39(2):93-5. · 1.25 Impact Factor
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ABSTRACT: Repair of nasal and auricular malformation remains an obstacle for clinicians because of poor regenerative capacity of cartilage and limitation of donor sites. In the current study, we developed a novel approach to regenerate implantable nasal alar cartilage by using marrow precursor cell (MPC) sheet and biodegradable scaffold of polylactic acid-polyglycolic acid copolymer (PLGA).
Rabbit MPCs were expanded and induced by transforming growth factor-beta to improve chondrocyte phenotype. MPC sheets were obtained by continuous culture and used to wrap PLGA scaffold in the shape of the human nasal alar. The constructs were incubated in a spinner flask for 4 weeks, and cartilage formation was investigated by gross inspection and histological examination. The constructs were then implanted subcutaneously into a nude mouse. Specimens were harvested and analyzed 4 weeks after implantation.
The results showed that cartilaginous tissue formed and PLGA absorbed during in vitro incubation. Histological analysis showed engineered cartilage consisted of evenly spaced lacunae embedded in a matrix rich in proteoglycans, and kept the initial shape of the nasal alar. Based on this "MPC sheet combining polymer strategy," implantable nasal alar could be successfully regenerated.
This strategy has the advantage of high cell transplantation efficiency and great potential for clinical application.
Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons 03/2009; 67(2):257-64. · 1.58 Impact Factor
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ABSTRACT: Treatment and management of tracheal defects remain challenges in head and neck surgery. The purposes of this study were to explore a novel strategy to fabricate tissue-engineered trachea by using chondrocyte macroaggregate, and evaluate the feasibility of creating tracheal cartilage equivalents grown in the shape of cylindrical structure without scaffold. Chondrocytes from rabbit cartilage were expanded and seeded into a culture dish at high density to form mechanically stable chondrocyte macroaggregate. Once the chondrocyte macroaggregate was harvested by scrapping technique, it was wrapped around a silicon tube and implanted subcutaneously into the cell donor rabbit. Eight weeks later, specimens were harvested and analyzed for gross appearance, and histological, biochemical, and biomechanical properties. These values were compared with native rabbit cartilage. It was found that expanded chondrocytes could be harvested as a coherent cellular macroaggregate and could be fabricated into a tubelike graft. After in vivo implantation, cartilage-like tissue with cylindrical structure was regenerated successfully. Histological analysis showed engineered trachea cartilage consisted of evenly spaced lacunae embedded in a matrix rich in proteoglycans; type II collagen was also highly expressed in this engineered trachea cartilage. In a conclusion, based on the chondrocyte macroaggregate strategy, tracheal cartilage equivalents with cylindrical shape could be successfully reconstructed. This construct has advantages of high cell-seeding efficiency, good nutritional perfusion, and minimal inflammatory reaction, which provided a highly effective cartilage graft substitute and could be useful in many situations of trachea-cartilage loss encountered in clinical practice.
Artificial Organs 12/2007; 31(11):826-34. · 2.00 Impact Factor
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ABSTRACT: The purpose of the current study is to fabricate tissue engineered trachea with poly-lactic-glycolic acid (PLGA) non-woven mesh enforced by collagen type I. PLGA fibres coated with collagen solution were put together and fabricated into the shape of a human trachea, after drying and cross-linking treatment, a non-woven mesh with "C" shape formed. Chondrocytes from sheep nasal septum cartilage were expanded in vitro and seeded into PLGA/collagen non-woven mesh in the density of 5.0 x 10(7)mL(-1). After 5 days of in vitro incubation, six Cell-PLGA/collagen composites were implanted subcutaneously into the back of 6 nude mice to prefabricate a tissue engineering trachea. Eight weeks later, the cartilage formation was observed by gross inspection and histological examination. Cartilage-like tissue in the shape of the initial PLGA/collagen scaffold had been regenerated successfully without obvious inflammatory response. The tissue engineered trachea cartilage consisted of evenly spaced lacunae embedded in matrix stained red with safranin-O staining. The amount of GAGs in tissue engineered trachea cartilage reached 71.42% of normal value in native cartilage. This study demonstrated that collagen-enforced PLGA non-woven mesh facilitated the adhesion and proliferation of chondrocytes, it also owned adequate mechanical strength to serve as an ideal scaffold for trachea tissue engineering without internal support.
British Journal of Oral and Maxillofacial Surgery 07/2007; 45(4):272-8. · 1.95 Impact Factor
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ABSTRACT: To develop a scaffold material containing collagen 1 and sodium hyaluronate for the cartilage tissue engineering and to evaluate its biocompatibility by using the rabbit chondrocytes derived from a mandibular condylar process.
The porous matrices containing collagen 1 and sodium hyaluronate were fabricated by the freeze-drying technique and were crosslinked by using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC). The microstructure of the scaffold was observed under the scanning electron microscope (SEM), and the enzymatic degradation test was performed to compare the ability of the scaffold resistance to collagenase before and after the crosslinking. The chondrocytes from the rabbits' condylar process were isolated and cultured before they were seeded into the scaffold, and cell attachment and proliferation were measured by the cell count 1, 3, 5, 7 and 10 days after the cell being seeded; then, the biocompatibility of the scaffold was evaluated by the light microscopic examination, histological examination, and the SEM exmination.
The porous structure of the scaffold facilitated the penetration and attachment of the seeded cells. The porosity was 83.7% and the pore size was 100-120 microm. The cell number increased from 3.7 x 10(4) per scaffold 1 day after the cell being seeded to 8.2 x 10(4) per scaffold 10 days after the cell being seeded. The crosslinking treatment could significantly enhance the scaffold resistance to the collagenase activity. The examinations under the light microscope and SEM indicated that the chondrocyte adhered and spread well on the scaffold, and the extracellular matrices were also observed around the chondrocytes.
The porous scaffold composed of collagen I and hyaluronan has an appropriate structure and a good biocompatibility for the attachment and proliferation of the chondrocytes, which can facilitate it to become a useful scaffold in the cartilage tissue engineering.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 05/2007; 21(4):401-5.
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ABSTRACT: Bone regeneration is often needed for many aesthetic and reconstructive procedures. Tissue engineering provided a promising approach to supplement existing treatment strategies. In this study, we aimed to evaluate the effect of reconstructing mandibular defect by using bioceramics seeded with bone marrow derived osteoblasts.
Canine's autologous marrow stromal cells were Culture-expanded and induced to osteoblastic phenotype, then were seeded into prepared porous beta-tricalcium phosphate, after being incubated in vitro. The cell/ scaffold complexes were implanted into the prepared defect in canines' mandibula and fixed by internal rigid fixation. In control groups, beta-tricalcium phosphate alone and autologous iliums were implanted into the prepared defects. Twelve weeks after implantation, the specimens were examined macroscopically and histologically.
In experimental group and autologous iliums group, new bone grafts were successfully developed at 12 weeks after implantation and repaired the continuity of the mandibula. Histologically, newly formed bone could be observed on the surface and in the pores of beta-tricalcium phosphate in the cell/scaffold group, whereas incomplete bone repair was found in pure beta-tricalcium phosphate group.
The harvested bone marrow derived osteoblasts possess the ability to form new bone tissue when seeded onto porous beta-tricalcium phosphate, which shows the potential of using this method to repair large segmental mandibular defect clinically.
Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery: TJTES 11/2006; 12(4):268-76. · 0.33 Impact Factor
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ABSTRACT: Pingyangmycin gelatin microspheres(PYM-GMS) was prepared by optimal double-phase emulsified condensation polymerization for the interventional Chemoembolization with carotid artery therapy, and its release characteristics were studied in vivo and in vitro. The results of three ways of administration(vein drop, artery perfusion and artery embolization) were compared. The experiment indicates that the diameter of PYM-GMS is more appropriate for the application in external carotid artery embolization with PYM-GMS, which significantly reduces the circulating drug level and the dosage, prolongs the time period of higher drug concentration, achieves the purpose of sustained release and targeted tumor therapy.
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi 01/2004; 20(4):646-9.
