Bao-Zhen Li

China Agricultural University, Beijing, Beijing Shi, China

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Publications (7)10.15 Total impact

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    ABSTRACT: Butane-oxidizing bacteria in soil sample sites from Puguang gas field in Sichuan province and Jianghan oil field in Hubei province were isolated and 16S rRNA gene sequence and phylogenetic analysis were applied. The differences of number and phylogenetic position and population diversity of hydrocarbon-oxidizing bacteria in different environment were investigated. The results show that 25 strains of butane-oxidizing bacteria were isolated. Based on sequencing of 16S rRNA gene, the species of bacteria in two samples are classified into 3 phyla including Firmicutes, Actinobacteria and Proteobacteria. The community structure of butane-oxidizing bacteria isolated from two oil samples is simple, both of them contain 4 genus including Bacillus, Pseudomonas, Rhodococcus and Arthrobacter. Strains in the genus of Ochrobactrum and Mycobacterium were only isolated from Puguang gas field. The number and population diversity of butane-oxidizing bacteria in Puguang gas field was more than those in Jianghan oil field.
    Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 01/2012; 33(1):299-304.
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    ABSTRACT: A Gram-negative, rod-shaped, aerobic, non-motile, bacterium (YG-17(T)) was isolated from eutrophic Taihu lake sediment. Colonies grown on YG agar plates were circular, convex, and yellow-colored. On the basis of 16S rRNA gene sequence similarity, strain YG-17(T) was shown to be closely related to Novosphingobium aromaticivorum (97.9%), followed by Novosphingobium stygium (97.5%), Novosphingobium subterranea (96.9%) and Novosphingobium taihuense (96.7%). The DNA-DNA relatedness values of strain YG-17 (T) to the most phylogenetically related species N. aromaticivorum and N. stygium were 14% and 21%, respectively. The G+C content of the genomic DNA was 63.2 mol%. The major respiratory quinone was ubiquinone-10 and major fatty acids were C(18:1) (ω7c), C(17:1) (ω6c) and C(14:0)2-OH. Sphingoglycolipids were present, and spermidine was detected as the major polyamine. According to comparative physiological and chemotaxonomic analysis, strain YG-17(T) is proposed to be a novel species of the genus Novosphingobium, with the name of Novosphingobium sediminis sp. nov. The type strain is YG-17(T) (NBRC 106119(T)= CGMCC 1.9114(T)).
    The Journal of General and Applied Microbiology 01/2012; 58(5):357-362. · 0.74 Impact Factor
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    ABSTRACT: In this study, three bacterial communities were obtained from 12 Leonardite samples with the aim of identifying a clean, effective, and economic technique for the dissolution of Leonardite, a type of low-grade coal, in the production of humic acid (HA). The biodegradation ability and characteristics of the degraded products of the most effective bacterial community (MCSL-2), which degraded 50% of the Leonardite within 21 days, were further investigated. Analyses of elemental composition, (13)C NMR, and Fourier transform infrared revealed that the contents of C, O, and aliphatic carbon were similar in biodegraded humic acid (bHA) and chemically (alkali) extracted humic acid (cHA). However, the N and carboxyl carbon contents of bHA was higher than that of cHA. Furthermore, a positive correlation was identified between the degradation efficiency and the increasing pH of the culture medium, while increases of manganese peroxidase and esterase activities were also observed. These data demonstrated that both alkali production and enzyme reactions were involved in Leonardite solubilization by MCSL-2, although the former mechanism predominated. No fungus was observed by microscopy. Only four bacterial phylotypes were recognized, and Bacillus licheniformis-related bacteria were identified as the main group in MCSL-2 by analysis of amplified 16S rRNA genes, thus demonstrating that Leonardite degradation ability has a limited distribution in bacteria. Hormone-like bioactivities of bHA were also detected. In this study, a bacterial community capable of Leonardite degradation was identified and the products characterized. These data implicate the use of such bacteria for the exploitation of Leonardite as a biofertilizer.
    Applied Microbiology and Biotechnology 11/2011; 93(6):2581-90. · 3.81 Impact Factor
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    ABSTRACT: Effects of phosphorus of low concentrations on the growth and the phosphorus removal efficiency of Scenedesmus obliquus were investigated in this study. Results showed that Scenedesmus obliquus achieved a phosphorus removal efficiency of 100% within 22 h when the initial algal cell concentration was 1 x 10(5) /mL and the initial phosphorus concentration was 0.02-0.10 mg/L. With the initial phosphorus concentration increased from 0.02 mg/L to 0.10 mg/L, both growth velocity of Scenedesmus obliquus and maximum biomass increased obviously. Research found that phosphorus concentration had a significant influence on cell morphology of algal. In the external phosphorus sufficient conditions, most of algae cell present as four cells gather round form, then transformed into two cells side by side form in the absence of external phosphorus in culture medium, Finally in single as the main form of existence.
    Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 11/2010; 31(11):2661-5.
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    ABSTRACT: A Gram-negative, yellow-pigmented bacterium, designated strain R2A-16(T), was isolated from sediment of Rupa Lake in Nepal and analysed using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain R2A-16(T) is affiliated to the genus Cloacibacterium of the family Flavobacteriaceae; 16S rRNA gene sequence similarity between strain R2A-16(T) and Cloacibacterium normanense CCUG 46293(T) was 98.07 %. The isolate contained iso-C(15 : 0) (35.6 %) as the major fatty acid and menaquinone MK-6 as the predominant respiratory quinone. The G+C content of the genomic DNA was 33.3 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain R2A-16(T) represents a novel species of the genus Cloacibacterium, for which the name Cloacibacterium rupense sp. nov. is proposed; the type strain is R2A-16(T) (=CGMCC 1.7656(T) =NBRC 104931(T)).
    International Journal of Systematic and Evolutionary Microbiology 10/2009; 60(Pt 9):2023-6. · 2.80 Impact Factor
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    ABSTRACT: A Gram-stain-positive, rod-shaped, endospore-forming, halophilic, alkalitolerant bacterium, designated halo-1(T), was isolated from sediment of Xiarinaoer soda lake, located in the Inner Mongolia Autonomous Region of China. Strain halo-1(T) grew in the presence of 9-30 % (w/v) NaCl (optimum 19 %) and at pH 5-10 (optimum pH 9). The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids of the isolate were anteiso-C(15 : 0) (58.35 %), anteiso-C(17 : 0) (12.89 %) and C(16 : 0) (6.52 %). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and a phospholipid of unknown structure. The DNA G+C content of the strain was 46.4 mol%. On the basis of 16S rRNA gene sequence similarity, strain halo-1(T) showed the highest similarity (93.9 %) to Salsuginibacillus kocurii CH9d(T). Strain halo-1(T) could be clearly differentiated from its closest phylogenetic relative on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain halo-1(T) represents a novel species, for which the name Salsuginibacillus halophilus sp. nov. is proposed, with the type strain halo-1(T) (=CGMCC 1.7653(T) =NBRC 104934(T)).
    International Journal of Systematic and Evolutionary Microbiology 09/2009; 60(Pt 6):1339-43. · 2.80 Impact Factor
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    ABSTRACT: Biomass wastewater contains plenty of cellulose, hemicellulose, starch, saccharide, organic acid and protein. It has high COD value and leads to environment pollution and resource waste. Biomass wastewater was treated in this investigate using a biological aerated filter (BAF) on laboratory scale, and the effect of treatment in different hydraulic retention time (HRT) had been studied. The results show that the optimum HRT is 10 h. The value of COD and TN in effluent is 74.90 mg/L and 1.21 mg/L respectively when run 10 h, which are under the highest concentration of the second category of pollutant discharge allowed in the state sewage discharge standard (GB 8978-1996). When the system is stabile, evaluating the population characteristics of dominating microorganism in BAF with biology analysis such as microbial screening, single bacteria evaluation and 16S rDNA sequences analysis. The dominant bacteria belonged to Pseudomonas sp., Acinetobacer sp., Stenotrophomonas sp., Rhodococcus sp.and Microbacterium sp..
    Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 12/2008; 29(11):3133-7.