I Oborná

Academy of Sciences of the Czech Republic, Praha, Hlavni mesto Praha, Czech Republic

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Publications (66)86.37 Total impact

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    ABSTRACT: The aim of this prospective study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage estimated by Sperm Chromatin Structure Assay. Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability. DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Although there were similar trends of rising DNA fragmentation index and ROS production among the three groups of men, the relationship did not reach statistical significance. Higher values of DNA fragmentation index and high DNA stainability may also reflect developmental and/or environmental problems and not only oxidative stress.
    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 09/2013; · 0.99 Impact Factor
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    ABSTRACT: Angiogenesis (new vessel formation from pre-existing ones) is important for growth and development, as well as in wound healing and tissue granulation, but also in growth and metastases of solid malignancies. Plants are rich in biologically active natural compounds and up to date, many of them, e.g. flavonolignans silybin and its derivatives, have been tested for various biological activities including anticancer and antiangiogenic properties. In the present study, 2,3-dehydrosilybin (DHS) and its galloylderivatives were tested for antiangiogenic properties in a variety of in vitro tests with human umbilical vein endothelial cells (HUVEC). Primary screening was performed using wound healing migration and MTT cytotoxicity tests. Subsequently, capillary-like tube formation of HUVEC on matrigel and cell proliferation were evaluated. In HUVEC cell migration test, minimal inhibitory concentrations around 40 µM for 3-galloyl-DHS, 2 µM for 7-galloyl-DHS, 10 µM for 20-galloyl-DHS, 6 µM for 23-galloyl-DHS and around 5 µM for DHS were observed. IC50 values 13.66 ± 1.58 µM for DHS, 14.49 ± 5.90 µM for 3-galloyl-DHS, 2.57 ± 0.84 µM for 7-galloyl-DHS, 11.88 ± 0.32 µM for 20-galloyl-DHS and 6.13 ± 1.38 for 23- galloyl-DHS were found using MTT cytotoxicity test. The same trend reflected also tube formation and proliferation tests. Consequently the best antiangiogenic properties were noted for 7-galloyl-DHS. Financial support from LF_2013_008, P301/11/0767 and NT11083 is acknowledged.
    The Students Scientific Conference on Biotechnology and Biomedicine; 04/2013
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    ABSTRACT: AIMS: Male infertility has been associated with imbalance in the polyunsaturated fatty acids arachidonic acid/docosahexaenoic acid (AA/DHA) ratio. This study aimed to assess whether use of the potent antioxidant, lycopene, would affect this imbalance in seminal plasma and to test whether modulation of the AA/DHA ratio correlates with male fertility. METHODS: The study included 44 males from infertile couples who were treated with lycopene for three months before scheduled IVF treatment. The control group was 13 fertile males who were also treated with lycopene for three months. AA and DHA levels were measured in seminal plasma before and after treatment. The outcome of IVF treatment after lycopene use in of males from infertile couples was recorded. RESULTS: The subjects were subdivided into 21 normozoospermic males from couples with idiopathic infertility and 23 males with semen abnormalities. Prior to the treatment with lycopene, the AA/DHA ratios in both subgroups of patients were significantly higher than in fertile controls and improved following treatment with lycopene. Improvement was more marked in the normospermic males. Seven spontaneous pregnancies (16%) occurred before the scheduled IVF treatment and 15 couples (42%) achieved pregnancy after IVF. CONCLUSIONS: Three months of treatment with lycopene led to a significant improvement in the AA/DHA ratio in seminal plasma of males from infertile couples and facilitated the spontaneous as well as IVF conception.
    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 02/2013; · 0.99 Impact Factor
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    ABSTRACT: AIMS: Caesarean section is the most common obstetric operation associated with short and long term risks, one of which is uterine scar dehiscence. In this case report we describe four cases of in vitro fertilization and embryo transfer (IVF + ET) treatment where the embryo was transferred into the uterus with known scar dehiscence in the lower uterine segment after a previous Caesarean section (SC). METHODS: All transfers of embryos were ultrasound guided directly into the middle of uterine cavity. All resulting pregnancies continued without problems related to the dehiscent scar and babies were delivered in the third trimester by elective/emergency SC. RESULTS: Our cases suggest that IVF + ET can be offered as an infertility treatment option despite a dehiscent scar in the lower uterine segment after previous SC.
    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 02/2013; · 0.99 Impact Factor
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    ABSTRACT: Objective: To assess and compare the frequency of selected gene mutations of thrombophilic markers (FV Leiden, FII prothrombin G20210A and MTHFR C677T) in patients with primary and secondary infertility.Design: Retrospective study.Setting: Institute of normal anatomy, Faculty of Medicine and Dentistry, Palacky University Olomouc.Methods: The study included 92 patients with primary infertility and 89 patients with secondary infertility. Indications for examination of these mutations were following: a positive family or personal history, a positive obstetrical history or a repeated failure of assisted reproduction treatment.Results: According to our anticipation, women with the secondary infertility were significantly older(p < 0.0005) than those with primary infertility. No mutations of genes of examined thrombophilic markers (FV, FII and MTHFR), either alone or in combination, were found in only 8.7 % patients with primary infertility and in 5.6 % patients with secondary infertility. Significantly higher frequency of factor Leiden(p < 0.02) was observed in women with secondary infertility. There were no significant differences in the frequency of detected mutations of the remaining factors. Conclusion: Based on our findings we suggest that the assessment of selected gene mutations of thrombophilic markers should be a part of the diagnostic algorithm in patients with positive history for thrombophilic disorders. Keywords: inherited thrombophilia - female infertility - IVF and ET.
    Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 02/2013; 78(1):73-7.
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    ABSTRACT: Objective of the study: To evaluate the success of treatment cycle after transfer of no early cleavage embryos in relation to the length of in vitro cultivation.Type of the study: Retrospective study.Setting: Dept. of Obstetrics and Gynecology, Faculty of Medicine and Dentistry, Palacky University and University Hospital Olomouc.Methods: The IVF cycles with transfer of no early cleavage (NEC) embryos, ie. embryos with a delayed cleavage were evaluated in relation to the length of in vitro cultivation. The total of 338 embryos were cultured for three days (group 3D) and 204 embryos were cultured for 5 days (group 5D). The input parameters of the patients in these two groups were: their age, the percentage of fertilized oocytes and the number of transferred embryos. As the output parameters the number of cancelled cycles, clinical pregnancy rate (PR/ET), implantation rate (IR), and the number of pregnancy losses (AB) were evaluated. Finally, the results of these pregnancies were also evaluated.Results: Both study groups had the same input parameters, except that fewer embryos were transferred in the group 5D than in 3D (1.8 ± 0.5 vs. 2.0 ± 0.5,p = 0.000). No cycles were cancelled in the group 3D while 33 cycles were cancelled in 5D (p = 0.000). The number of clinical pregnancies and implantation rate in the group 5D was significantly higher than in 3D (PR/ET 50% vs. 37%, IR 36% vs. 25%, p = 0.001). The groups did not statistically significantly differ in the number of pregnancy losses, or in the number of pregnancies that resulted in childbirth.Conclusion: In the 5D group with the longer in vitro cultivation statistically more pregnancies and implanted embryos were achieved, although the number of pregnancies that resulted in childbirth did not differ between groups. Keywords: embryo development - pregnancy - IVF - pregnancy outcomes.
    Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 02/2013; 78(1):68-72.
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    ABSTRACT: Angiogenesis is a normal and vital process in growth and development, as well as in wound healing and in tissue granulation. However, it is also a fundamental step in the growth of solid tumors and their transformation from a dormant state to a malignant one, leading to the research of angiogenesis inhibitors. Photosynthesizing organisms are a rich source of biologically active natural products and up to date, many phytochemicals have been tested for antiangiogenic properties [1]. One interesting polyphenolic compound has been recently identified in cyanobacteria as 2,2’-bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)-cyclopent-4-ene-1,3-dione] nostotrebin 6 (NOS-6). NOS-6 is an effective acetylcholinesterase and butyrylcholinesterase inhibitor and its cytotoxicity and pro-apoptotic activity has recently been evaluated in mouse fibroblasts BALB/c by our group [2]. In the present study, NOS-6 and a synthetic cyclopentenedione derivative TX-1123 [3] were tested for antiangiogenic activity in a variety of in vitro tests with human umbilical vein endothelial cells (HUVEC). Primary screening was performed using MTT cytotoxicity and wound healing migration tests. Subsequently, capillary-like tube formation of HUVEC on Matrigel, and cell proliferation were evaluated. Both compounds exhibited cytotoxic properties towards the HUVEC with IC50 values 14.6 ± 0.7 (NOS-6) and 10.5 ± 0.4 (TX-1123) µM. In HUVEC cell migration test, minimal inhibitory concentrations around 15 and 8 µM were observed for NOS-6 and TX-1123, respectively. Using Matrigel tube formation inhibition test, significant inhibition by TX-1123 was noted already at 0.5 µM (81.7 ± 9.7 % of control). In contrast, NOS-6 was virtually without effect up to 10 µM and significant effect was observed at 20 µM (71.4 ± 3.1 % of control). Finally, the compounds tested inhibited HUVEC proliferation with IC50 values 3.81 ± 0.04 and 4.3 ± 0.3 µM for NOS-6 and TX-1123, respectively. In conclusion, TX-1123 exhibited higher anti-angiogenic activity compared to NOS-6. LF UP Institutional support is acknowledged. References: 1. S. C. Gupta, J. H. Kim, S. Prasad, et al., Cancer Metastasis Rev 29, 405 (2010). 2. J. Vacek, J. Hrbac, J. Kopecky, et al., Molecules 16, 4254 (2011). 3. H. Hori, H. Nagasawa, M. Ishibashi, et al., Bioorg Med Chem 10, 3257 (2002).
    FEBS WORKSHOP. Molecular and Cellular Mechanisms in Angiogenesis; 10/2012
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    ABSTRACT: Angiogenesis is a complex physiological process through which formation of new blood vessels occurs from pre-existing ones. Its role is essential in ovulation, embryonic growth, placenta development, wound and injury healing and especially in growth and metastases of most solid malignancies. Excessive or insufficient angiogenesis is connected with many different human diseases (e.g. macular degeneration, rheumatoid arthritis, psoriasis, cancer, infertility, ulcers, heart diseases, obesity, teratogenesis). Plant kingdom is a rich source of biologically active natural compounds and up to date, many phytochemicals have been tested for antiangiogenic properties. Flavonolignan silybin, isolated from milk thistle (Silybum marianum), is a popular flavonoid possessing multiple biological activities operating at various cell levels including anticancer and antiangiogenic properties. We have recently demonstrated an influence of galloyl moiety in position 7 of silybin resulting in increase of its biological (antiangiogenic) activity (1). In the present study, a minor constituent of milk thistle extract (2,3-dehydrosilybin, DHS) and a series of its methylated and di-methylated derivatives were tested for antiangiogenic activity in a variety of in vitro tests with human umbilical vein endothelial cells (HUVEC). Primary screening was performed using MTT cytotoxicity and wound healing migration tests. Subsequently, capillary-like tube formation of HUVEC on Matrigel and cell proliferation were evaluated. In HUVEC cell migration test, minimal inhibitory concentrations around 10 µM were observed for all these compounds except 3,7-dimethyl-DHS, where only partial inhibition was observed at 50 µM. Similar results were obtained using Matrigel tube formation inhibition test with significant inhibition by DHS already at 5 µM (93.7 ± 2.0 % of control). In contrast, 3,7-dimethyl-DHS was virtually without effect up to 30 µM and quite slight (although significant) effect was observed for 3-methyl-DHS (70.3 ± 11.8 % at 20 µM). Finally, all the compounds tested inhibited HUVEC proliferation with IC50 values 5.6 ± 0.28, 4.1 ± 0.45, 5.1 ± 0.27, 7.4 ± 0.14, 4.4 ± 0.34, 7.3 ± 0.66, 5.3 ± 0.11, 2.7 ± 0.32 µM for DHS, 3-methyl-DHS, 5-methyl-DHS, 7-methyl-DHS, 20-methyl-DHS, 3,7-dimethyl-DHS, 3,20-methyl-DHS and 7,20-methyl-DHS, respectively. From these results, we can conclude that both 3- and 7-hydroxy groups are required for antiangiogenic activity of 2,3-dehydrosilybin. 1. Gazak, R.; Valentova, K.; Fuksova, K.; Marhol, P.; Kuzma, M.; Medina, M. A.; Oborna, I.; Ulrichova, J.; Kren, V., Synthesis and antiangiogenic activity of new silybin galloyl esters. J Med Chem 2011, 54, (20), 7397-407. Supported by grants P207/10/0288 (RG), P301/11/0662 (VK) from the Czech Science Foundation and LF UP Institutional support (KV, JU).
    Natural Anticancer Drugs; 06/2012
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    ABSTRACT: Functional 3D training originating in rehabilitation and physiotherapy routines, is modelled on the basis of a patient's functional impairment and muscle function test results. 3-D functional rehabilitation comprises two key components. The first is three-dimensional movements of the body and the second is restoration of function, The aim of 3D training is not only to improve physical fitness and muscular coordination, it is recommended for all age groups regardless of health status. The aim of this review is to highlight the main principles, roles and methods of 3D functional training from a clinically-anatomical perspective and its application in clinical practice. Second, we present a case study of 3d training in an obese boy. Database search using the lemmatization method and data bases: Web of Knowledge, Web of Science, UpToDate, PubMed and Google Scholar for the years 2005-2011. Functional 3D training is an effective method for, among others, strengthening the postural muscles of the human body, increasing articular stability, strengthening the ligaments and stability of muscle groups,especially the back muscles and for improving lung capacity. The method of functional training is of considerable significance in activating the pelvic floor muscles and the pelvic-trochanteric muscles for gynegymnastics and incontinence prevention. It has application in the prevention of falls in patients with neurodegenerative diseases and, as shown below, in the therapy of obesity.
    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 03/2012; 156(1):63-9. · 0.99 Impact Factor
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    ABSTRACT: Silybin is a popular flavonolignan isolated from milk thistle (Silybum marianum), possessing multiple biological activities operating at various cell levels including anticancer and antiangiogenic properties. As the ability of silybin against angiogenesis is quite poor, we tried to improve it by its gallolyation. In the present study, a series of silybin gallates were tested for antiangiogenic activity in a variety of in vitro tests with human umbilical vein endothelial cells (HUVEC). Primary screening was performed using MTT cytotoxicity and wound healing migration tests. Subsequently, capillary-like tube formation of HUVEC on Matrigel and cell proliferation were evaluated for the most potent angiogenesis inhibitors. Silybin itself exhibited quite poor activities, inhibition of cell migration was observed in concentrations higher than 50 µM. Mono-galloylation of silybin 7-hydroxyl group decreased the minimal inhibitory concentration (MIC) to 10-20 µM. MICs for isomeric 3-O-galloylsilybin was 50 µM, for 20-O-galloylsilybin, 20 µM, and for 23-O-galloylsilybin, 20 µM. Similar results were obtained using Matrigel tube formation inhibition test with 7-O-galloylsilybin being the most active with significant inhibition at 10 µM (79.9 % of control). In contrast, 3-O-galloylsilybin was virtually without effect up to 75 µM and only very slight (although significant) effect was observed for 23-O-galloylsilybin (81.5 % at 20 µM) and 20-O-galloylsilybin (94.5 ± 4.1% of control at 20 µM and 49.5 ± 2.0 % at 30 µM). Finally, all the compounds tested inhibited HUVEC proliferation with IC50 values 9.99 ± 0.11, 12.31 ± 0.27, 4.75 ± 0.13, 10.35 ± 0.35 and 11.59 ± 0.71 µM for silybin, 3-O-galloylsilybin, 7-O-galloylsilybin, 20-O-galloylsilybin and 23-O-galloylsilybin, respectively. From these results, we can conclude that 7-hydroxy group is the most important for antiangiogenic activity of silybin. Supported by MSM 6198959216, P207/10/0288 (R.G.), P301/11/0767 (J.U.) and AV0Z50200510.
    5th International Conference on Polyphenols and Health; 10/2011
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    ABSTRACT: The synthesis of various silybin monogalloyl esters was developed, and their antiangiogenic activities were evaluated in a variety of in vitro tests with human umbilical vein endothelial cells (HUVECs). A structure-activity relationship (SAR) study found the regioselectivity of the silybin galloylation to be highly significant. Silybin (as an equimolar mixture of two diastereomers A and B) exhibited quite poor antiangiogenic activities, whereas its B stereoisomer is more active than silybin A. The galloylation of phenolic OH groups of natural silybin (a mixture of both isomers) leads to increases in their antiangiogenic activities, which is more apparent with the 7-OH than the 20-OH. In contrast, gallates at aliphatic OH groups either had a comparable activity to the parent compound or are even worse than silybin, which was observed in the case of 3-O-galloylsilybin. The most effective compound from this series (7-O-galloylsilybin) has also been prepared from stereochemically pure silybins A and B to evaluate the effect of stereochemistry on the activity. As with silybin itself, the B isomer of 7-O-galloylsilybin was more active than the A isomer.
    Journal of Medicinal Chemistry 09/2011; 54(20):7397-407. · 5.61 Impact Factor
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    ABSTRACT: The formation of new capillaries through angiogenesis is an important phenomenon involved in normal growth and healing processes, but also in pathological conditions such as solid tumor progression, metastasis, diabetic retinopathy, hemangiomas, arthritis, psoriasis and atherosclerosis. Flavonolignan silybin, isolated from milk thistle (Silybum marianum), is a popular flavonoid possessing multiple biological activities operating at various cell levels including anticancer and antiangiogenic properties. In the present study, several natural and synthetic derivatives of silybin and 2,3-dehydrosilybin were tested for antiangiogenic activity in a variety of in vitro tests with human umbilical vein endothelial cells (HUVEC). Primary screening was performed using MTT cytotoxicity and wound healing migration tests. Subsequently, capillary-like tube formation of HUVEC on Matrigel and cell proliferation was evaluated for the most potent angiogenesis inhibitors. Silybin itself, as well as isosilybin (both A and B stereoisomers) exhibited quite poor activities, inhibition of cell migration was observed in concentrations higher than 50 µM. Esterification of its hydroxyl groups with palmitoic acid did not lead to improvement of HUVEC migration inhibitory activity. In contrast, mono-galloylation of silybin 7-hydroxyl group decreased the minimal inhibitory concentration (MIC) to 10-20 µM. MICs for isomeric 3-O-galloylsilybin was 50 µM, for 20-O-galloylsilybin, 20 µM, and for 23-O-galloylsilybin, 20 µM. Similar results were obtained using Matrigel tube formation inhibition test with 7-O-galloylsilybin being the most active with significant inhibition already at 10 µM (79.9 ± 3.7 % of control). In contrast, 3-O-galloylsilybin was virtually without effect up to 75 µM and only very slight (although significant) effect was observed for 23-O-galloylsilybin (81.5 ± 15.9 % of control at 20 µM) and 20-O-galloylsilybin (94.5 ± 4.1% of control at 20 µM and 49.5 ± 2.0 % at 30 µM). 2,3-Dehydrosilybin and its methoxy-derivatives were the most active from all the compounds studied. In HUVEC cell migration test, MICs around 10 µM were observed for all these compounds except 3,7-dimethoxy-2,3-dehydrosilybin, where only partial inhibition was observed at 50 µM. No inhibitiory effect of this compound was observed in Matrigel tube formation assay either. From these results, we can conclude that both 3- and 7-hydroxy groups are required for antiangiogenic activity of (2,3-dehydro)silybin. Supported by grant MSM 6198959216, P207/10/0288 (R.G.) from the Czech Science Foundation and by Research concept of the Institute of Microbiology AV0Z50200510.
    Nová léčiva závažných lidských onemocnění; 05/2011
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    ABSTRACT: The aim of present study was to investigate the effects of antioxidant lycopene on soluble receptor for advanced glycation end products (sRAGE) levels in blood and seminal plasma in normospermic males. Study included 15 fertile volunteers and 13 normospermic male partners from infertile relationships. The treatment was 12-week administration of 20 mg of lycopene or placebo followed by crossover and treatment for a further 12 weeks. The ELISA kit Quantikine(®) was used to determine sRAGE levels. Lycopene administration decreased sRAGE levels in seminal plasma in fertile volunteers (controls) as well as in male partners in the infertile relationships group (P=0.008 and P=0.012, respectively). No significant effect of lycopene on sRAGE in blood plasma was found in either group, but seminal plasma sRAGE was significantly suppressed. Lycopene decreased sRAGE in seminal, but not in blood plasma. This may be because of selective local uptake of lycopene in the male reproductive tract, namely in prostate. Decreased sRAGE may be caused by lycopene suppression of oxidative stressors and explain in part the putative improvement in fertility reported after lycopene treatment.
    American Journal Of Reproductive Immunology 01/2011; 66(3):179-84. · 3.32 Impact Factor
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    ABSTRACT: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. Prospective study. University hospital. Thirty-one fertile volunteers and 166 men undergoing fertility assessment were included. Aliquots of sperm suspension in phosphate-buffered saline solution were used for the reactive oxygen species (ROS) detection by chemiluminescence and for the detection of H(2)O(2) and peroxynitrite by flow cytometry, with use of specific fluorescent probes, carboxy-2',7'-dichlorodihydrofluorescein diacetate dye for H(2)O(2) and dihydrorhodamine 123 for peroxynitrite. Gated analysis determined the relative contribution of spermatozoa, leukocytes, and "other round cells." Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitrite production. The levels of ROS production measured by chemiluminescence and flow cytometry were related. Each cell type in semen contributed differently to the global intracellular levels of H(2)O(2) and peroxynitrite.
    Fertility and sterility 12/2010; 94(7):2604-8. · 3.97 Impact Factor
  • Ivana Oborna, Radim Licenik, Zdenek Mrozek
    The Lancet 06/2010; 375(9731):2071-2. · 39.21 Impact Factor
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    ABSTRACT: To estimate the incidence of bacteriospermia, the representation of specific micro-organisms and the generation of reactive oxygen species (ROS) in the semen of males from infertile couples in comparison with the semen of fertile volunteers. Males from infertile couples were divided according to WHO criteria of their spermiogram results into those with normospermia (Group A, n = 65) and those with semen abnormalities (Group B, n = 116). The control group consisted of 44 fertile volunteers (Group C, n = 44). Aerobic culture was performed to ascertain the genus and species of the present microorganisms. ROS production was estimated by the chemiluminescence method. Statistical analysis was performed using the Mann-Whitney test. The incidence of positive cultures in the semen between Groups A, B and C (69 %, 74 % and 66 %, respectively) did not differ significantly, with Staphylococcus and Streptococcus species being most frequently identified. A significant difference was found only between the occurrence of microorganism Escherichia coli in the groups A, B and C (11.1 %, 9.3 % and 3.2 %). There were no statistically significant differences between ROS production in semen with positive or negative culture in any of the studied groups. In all the studied groups, the incidence of bacteriospermia as well as ROS production in the semen were similar. Staphylococcus and Streptococcus species were found in all groups. A significantly higher frequency of Escherichia coli in the semen samples of males from infertile couples in comparison with those from fertile volunteers was observed. No differences in ROS production in semen samples with positive and negative culture results were found in any of the studied groups.
    Klinicka mikrobiologie a infekcni lekarstvi 12/2009; 15(6):192-5.
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    ABSTRACT: To determine Reactive Oxygen Species (ROS) production in neat semen and spermatozoa suspension using chemiluminescence and to examine correlation between both methods. Prospective laboratory study. Department of Obstetric and Gynecology, University Hospital, Olomouc. The study included fertile volunteers (FV, n = 17), men from infertile couples (NM, n = 19) and men with idiopathic infertility (NMI, n = 15). ROS levels were determined by the same method in neat and washed semen samples. The ROS production in neat semen was lower than that in spermatozoa suspension. There was no significant diference in ROS production between volunteers and males from infertile couples. There was a significant correlation between log ROS in neat semen and in spermatozoa suspension in studied groups (FV r = 0.85, p = 1.5 x 10(-5); NM r = 0.76, p < 2 x 10(-4); NMI r = 0.75, p < 1.5 x 10(-3)). Measurement of ROS in neat semen is simpler, faster and better reflecting the actual level of oxidative stress than the same measurement in spermatozoa suspension. The implementation of this method can complement the algorithm of diagnostics and treatment of male infertility and be helpful in selection of patients for antioxidant or antibiotic treatment.
    Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 12/2009; 74(6):399-403.
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    ABSTRACT: Reactive oxygen species (ROS) are essential for sperm function. However, excessive ROS production can impair sperm function and might be a factor contributing to male infertility. We investigated the levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) as well as lipid peroxidation, as represented by thiobarbituric acid reactive species (TBARS), in blood and seminal plasma of 38 normozoospermic males from infertile couples (NSI-males), compared with that of 17 fertile volunteers (FV-males). TBARS levels in blood and seminal plasma were higher in NSI-males than in FV-males (P < 0.0002, P < 0.0003, respectively), as were AA levels (P < 0.0003, P < 0.00004, respectively). On the contrary, the blood and seminal plasma levels of DHA were lower in NSI-males than in FV-males (P < 0.02 and P < 0.05, respectively). The AA/DHA ratios in blood and seminal plasma were higher in NSI-males than in FV-males (P < 0.003, P < 0.0007, respectively). Significant correlations between seminal and blood plasma levels of TBARS (P < 0.0001, r = 0.548), AA (P < 0.0001, r = 0.571) and DHA (P < 0.0001, r = 0.506) were found. Our data provide new insight into lipid metabolism in male infertility and indicate that systemic oxidative stress resulting in increased lipid peroxidation and an altered fatty acid profile may be, at least in part, responsible for infertility even in normozoospermic males.
    Human Reproduction 11/2009; 25(2):308-16. · 4.67 Impact Factor

Publication Stats

154 Citations
86.37 Total Impact Points

Institutions

  • 2011
    • Academy of Sciences of the Czech Republic
      • Hydrobiologický ústav
      Praha, Hlavni mesto Praha, Czech Republic
  • 2004–2011
    • Palacký University of Olomouc
      • Department of Obstetrics and Gynecology
      Olomouc, Olomoucky kraj, Czech Republic
  • 2007–2009
    • University Hospital Olomouc
      • Department of Obstetrics and Gynecology
      Olmütz, Olomoucký, Czech Republic