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Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 07/2010; 31(7):826-7.
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ABSTRACT: The effects of pentabrominated diphenyl ethers (BDE-99) were examined on mRNA and protein expressions of c-Myc and p53 in breast cancer MCF-7 cells. Human breast cancer MCF-7 cells were cultured in Dulbecco's modified Eagle's medium. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay was employed to investigate cell proliferation. Real time-polymerase chain reaction (RT-PCR) and immunohistochemistry were performed to detect mRNA and protein expressions of c-Myc and p53. At the concentration of 1 × (10−4–10−8) mol L−1, BDE-99 stimulated proliferation of MCF-7 cells in a concentration- and time-dependent manner. RT-PCR and immunohistochemistry analyses revealed that treatment with 1 × (10−4–10−7) mol L−1 for 72 h resulted in significantly increased mRNA and protein expressions of c-Myc and inhibited p53. It is possible that BDE-99 exerted estrogenic actions and might markedly stimulate proliferation in estrogen responsive breast cancer MCF-7 cells. BDE-99 similar to estrogen promoted c-Myc mRNA and protein, and inhibited p53 mRNA and protein expression in MCF-7 cell.
Toxicological & Environmental Chemistry. 07/2010; 92(6):1177-1185.
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Yin Yin Xia,
Dai Yu Hu,
Fei Ying Liu,
Xiao Meng Wang,
Yan Li Yuan,
De Hua Tu,
Yi Xin Chen,
Lin Zhou,
Li Zhen Zhu,
Wei Wei Gao,
Hong Yuan Wang,
Da Fang Chen,
Li Yang,
Ping Ping He,
Xiao Ting Li,
Ying Jian He,
Feng Sun,
Si Yan Zhan
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ABSTRACT: More than 1 million tuberculosis (TB) patients are receiving the standard anti-TB treatment provided by China National Tuberculosis Prevention and Control Scheme (CNTS) in China every year. Adverse reactions (ADRs) induced by anti-TB drugs could both do harm to patients and lead to anti-TB treatment failure. The ADACS aimed to explore ADRs' incidences, prognoses, economical and public health impacts for TB patients and TB control, and build a DNA bank of TB patients.
Multiple study designs were adopted. Firstly, a prospective cohort with 4488 sputum smears positive pulmonary tuberculosis patients was established. Patients were followed up for 6-9 months in 52 counties of four regions. Those suspected ADRs should be checked and confirmed by Chinese State Food and Drug Administration (SFDA). Secondly, if the suspected ADR was anti-TB drug induced liver injury (ATLI), a nested case-control study would be performed which comprised choosing a matched control and doing a plus questionnaire inquiry. Thirdly, health economical data of ADRs would be collected to analyze financial burdens brought by ADRs and cost-effectiveness of ADRs' treatments. Fourthly, a drop of intravenous blood for each patient was taken and saved in FTA card for DNA banking and genotyping. Finally, the demographic, clinical, environmental, administrative and genetic data would be merged for the comprehensive analysis.
ADACS will give an overview of anti-TB drugs induced ADRs' incidences, risk factors, treatments, prognoses, and clinical, economical and public health impacts for TB patients applying CNTS regimen in China, and provide suggestions for individualized health care and TB control policy.
BMC Public Health 01/2010; 10:267. · 2.00 Impact Factor
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ABSTRACT: To investigate the distribution of glutathione-S-transferase M1 (GSTM1) and T1 (GSTT1) genes polymorphisms in Chinese population and smear-positive pulmonary tuberculosis cases of Jilin province.
Articles about GSTM1 and GSTT1 genes polymorphisms published before 2009 in China were searched. The study population was obtained from fourteen counties (or districts) of Jilin province, which included all cases from November, 2007 to May, 2008, totally 1120. The genotypes of GSTM1 and GSTT1 were detected by multiplex PCR technique.
The frequencies of GSTM1 and GSTT1 'null' genotypes and combination M1-T1 'null' genotype acquired from systematic review were 54.2%, 46.8% and 26.2%, respectively, in Chinese Hans they were 53.4%, 44.9% and 25.5%, and in our research they are 57.2%, 20.4% and 13.7%, respectively. No significant differences between the frequencies of males and females as well as among that of different age groups were observed (P > 0.05). The frequency of GSTM1 'null' genotype in our research is slightly higher than that in systematic review (P = 0.016) , and the frequencies of GSTT1 'null' genotype and combination M1-T1 'null' genotype and are significantly lower than those in systematic review (both P < 0.001).
The frequencies of GSTM1 and GSTT1 'null' genotypes were different among ethnic. The statistical difference between systematic review and our research may due to our large sample size and mostly Southern people in previous studies.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 05/2009; 30(5):502-6.
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ABSTRACT: To evaluate effect of NaFeEDTA on serum ferritin level in iron deficient epidemic population.
A comprehensive literature retrieval was performed via searching electronic databases, hand searching bibliographies of books and relevant journals, collecting grey literatures, looking into conference abstracts, contacting fields experts and reviewing references and citations. Criteria from Cochrane EPOC review group were used to assess the quality of the included studies. Generic inverse variance method was used to undertake Meta-analysis.
The pooled estimate for serum ferritin level (weighted mean difference) was 1.58 microg/L (95% CI 1.20-2.09; P < 0.001).
This systematic review indicates that NaFeEDTA might improve serum ferritin concentration significantly in iron deficient epidemic population.
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 07/2008; 42(6):437-41.
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ABSTRACT: To explore the effects of nonylphenol on brain gene expression profiles in F1 generation rats by microarray technique.
mRNA was extracted from the brain of 2-day old F1 generation male rats whose F0 female generation was either exposed to nonylphenol or free from nonylphenol exposure, and then it was reversely transcribed to cDNA labeled with cy5 and cy3 fluorescence. Subsequently, cDNA probes were hybridized to two BiostarR-40S cDNA gene chips and fluorescent signals of cy5 and cy3 were scanned and analyzed. Results Two genes were differentially down-regulated.
Nonylphenol may disturb the neuroendocrine function of male rats when administered perinatally.
Biomedical and Environmental Sciences 03/2008; 21(1):1-6. · 1.35 Impact Factor
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ABSTRACT: To evaluate the effect of NaFeEDTA on hemoglobin level in iron deficient population.
Comprehensive literature retrieval was performed via searching electronic databases, hand searching bibliographies of books and relevant journals, collecting grey literatures, looking into conference abstracts, contacting fields experts and reviewing references and citations. Criteria from Cochrane EPOC review group were used to assess the quality of included studies. Generic inverse variance method was used to undertake meta-analysis.
The pooled estimate for hemoglobin level (weighted mean difference) was 12.14 g/L (95% CI: 5.60-18.69; P < 0.001). Subgroup analysis indicated that lower baseline hemoglobin level and higher dose for intervention were associated to greater increase in hemoglobin level.
This systematic review indicated that NaFeEDTA improved hemoglobin significantly in iron deficient population.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 02/2008; 29(1):65-70.
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ABSTRACT: To study the incidences of adverse reactions induced by anti-tuberculosis drugs in China.
Articles about adverse drug reactions (ADR) induced by anti-tuberculosis drugs published in 1996 - 2005 in China were searched. The incidences, possible risk factors, and prognoses of these ADR were analyzed.
According to our searching strategy and including criteria, 117 studies were included. The overall incidence of anti-tuberculosis drug induced ADR of these studies was 12.62%, and the overall incidence of hepatic injury was 11.9%, which was the highest among all kinds of ADR induced by anti-tuberculosis therapy. For different types of study, different diagnostic standards of hepatic injury, and different study institutions, the reported incidences of hepatic injury varied. Retrospective cohort studies showed that HBV(+) tuberculosis patients had a significantly higher risk of hepatic injury than HBV(-) patients. The prognosis of hepatic injury was good; 85.84% patients were cured of hepatic injury according to the articles which reported outcomes. The whole study was finished by 2006.
The incidence of adverse reactions induced by anti-tuberculosis drugs is high in China. Prevention and treatment of ADR are very important for improving the adherence of patients.
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 07/2007; 30(6):419-23.
