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Publications (8)0 Total impact

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    ABSTRACT: The authors have revealed the acutest problems of laboratory tuberculosis diagnosis service in the Russian Federation. They have made their conclusion on this issue and developed the following recommendations: to continue the reorganization of Russia's laboratory tuberculosis diagnosis service; to reconstruct and repair laboratories; to provide them with up-to-date equipment; to make a full-scale training of laboratory workers; to standardize the procedures of microbiological tests for tuberculosis; to organize bacteriological tuberculosis service monitoring in Russia, including monitoring of tuberculosis-controlling facilities; to develop and officially approve a number of normative documents (current standards for the time spent on working operations and criteria for estimating the load on the personnel performing the procedures of a study, new staff lists for laboratories, the level and structure of salaries in the laboratory's personnel).
    Problemy tuberkuleza i bolezneĭ legkikh 02/2006;
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    ABSTRACT: The experience of researchers of the Central Research Institute of Tuberculosis, Russian Academy of Medical Sciences, in the pilot areas of Russia has made it possible to perfect approaches to improving a bacteriological diagnosis of tuberculosis in general health care facilities. A decisive role in enhancing the effectiveness of detection of patients with tuberculosis by the Ziel-Nielsen test is played by the following factors: training of laboratory personnel; provision of laboratories with high-grade equipment and reagents; introduction of the methods and techniques unified and approved by the Ministry of Health of the Russian Federation; regular supervision of jurisdictional laboratories; outside control of the quality of studies. Proper organization of examination of patients, including an effectual selection of patients to be examined, the observance of the established frequency of their examination, and provision of the required quality of a taken sample greatly affect the efficiency of a microscopic diagnosis of bacillary patients. In addition, the centralization of performed microbiological tests for tuberculosis in the most trained, certified, and licensed large laboratories exercising a centralized control of the quality of microbiological studies is optimal.
    Problemy tuberkuleza i bolezneĭ legkikh 02/2004;
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    ABSTRACT: The practice of the researchers of the Central Research Institute of Tuberculosis in Russia's experimental areas yielded approaches to improving the microbiological diagnosis of tuberculosis in both specialized and general health care facilities. A decisive role in enhancing the efficiency of microbiological identification of patients with tuberculosis is played by the following factors: laboratory staff training, provision of laboratories with qualitative equipment and reagents, introduction of unified methods and those approved by the Ministry of Health of the Russian Federation, regular supervision of jurisdictional laboratories, and outside test quality control. The efficiency of microbiological diagnosis of bacillary patients primarily depends on the expediency of formation of groups of patients under study, the observance of the frequency of examinations, and the assurance of the quality of collected materials. With this, the centralization of microbiological tests for tuberculosis at the most trained, certified, and licensed large laboratories that exercise a centralized control over the quality of microbiological tests.
    Problemy tuberkuleza 02/2002;
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    ABSTRACT: The authentic data on the drug resistance of Mycobacterium tuberculosis can be obtained only provided that standard laboratory procedures and the unified standardized method for determining the resistance are used along with a compulsory outside quality control over the performance of this test. The supervisory activities of the researchers of the Central Research Institute of Tuberculosis (CRIT), Russian Academy of Medical Sciences, made in some regions of Russia included standardization of methods and their implementation quality control allowed them to reveal and to correct main errors in drug resistance testing. In this connection, this made it to record the true level of M. tuberculosis resistance to essential antituberculous agents in the areas investigated by the researchers of CRIT. Analyzing the data on the drug resistance of M. tuberculosis in the above areas leads to the conclusion that 2000 was marked by rather high rates of primary drug resistance; nearly a fourth of all new and prior cases of pulmonary tuberculosis studied for its drug resistance were carriers of a M. tuberculosis resistant to at least one antituberculous agent.
    Problemy tuberkuleza 02/2002;
  • Problemy tuberkuleza 02/2001;
  • V I Golyshevskaia, E V Sevast'ianova, G A Voronina
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    ABSTRACT: The studies have showed the frequency and spectrum of drug resistance, determined by the methods of proportions and absolute concentrations, to be virtually equal. This suggests that both methods are of equal diagnostic value. The method of absolute concentrations is rather accurate and similar to that of proportions in sensitivity, by ensuring comprehensive determination of the spectrum of drug resistance. Furthermore, the advantages of this method are its simplicity, accessibility, and cost-efficiency.
    Problemy tuberkuleza 02/2001;
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    ABSTRACT: Multiresistant M. tuberculosis strains show varying drug resistance, virulence and growth rates. The count of cells of some multiresistant strains in the guinea-pig parenchymatous organs after intracardiac inoculation was comparable with that of after inoculation with clinical isolates. Furthermore, some multiresistant strains were not inferior to sensitive clinical isolates. The findings lead to the conclusion that there is a wide range in the virulence of multiresistant strains and hence they can present an epidemiological hazard.
    Problemy tuberkuleza 02/1999;
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    ABSTRACT: The results of determination of drug medium depend on both individual cultural properties of the strains tested and the type of the medium used. The composition of a medium is of significance for some strains: the latter grow in any medium and are characterized by the same spectrum of resistance. However, most drug-resistant strains have higher feeding demands which are not fully met on the lyophilized Löwenstein-Jensen medium. The strains in the lyophilized medium show an incomplete spectrum of resistance and less grow than do those in freshly prepared Löwenstein-Yersen medium.
    Problemy tuberkuleza 02/1999;