Guo-Li Shen

Hunan University, Ch’ang-sha-shih, Hunan, China

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Publications (289)971.75 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Noble metal nanoparticles are currently of great interest because of their unique optical properties and potential applications in disease diagnostics and cancer treatment. In the present work, a discovery was reported that dsDNA with terminal thiols at its two ends could lie easily flat onto the gold nanoparticle (GNP) surface rather than cross linked different GNPs, indicating an unique self-assembly behavior of newly-designed molecules on GNPs. This could intensively stabilize gold nanoparticles against aggregation even at a high salt concentration. On the basis of this discovery, a novel light-up colorimetric sensing strategy was developed for the detection of p53 gene by combining with the cyclical nucleic acid strand-displacement polymerization (CNDP). For the described colorimetric system, GNPs require no any surface functionalization, and target recognition reaction and CNDP amplification could be conducted under the optimized conditions to achieve a high efficiency. The high detection sensitivity and desirable selectivity are achieved, and the potential practical application was demonstrated. Besides, this sensing system can function in a wide range of salts, making it a suitable platform to cooperate with many biological processes.
    Biosensors & Bioelectronics 09/2014; 64C:292-299. · 6.45 Impact Factor
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    ABSTRACT: DNA strand displacement cascades have been engineered to construct various fascinating DNA circuits. However, biological applications are limited by the insufficient cellular internalization of naked DNA structures, as well as the separated multicomponent feature. In this work, these problems are addressed by the development of a novel DNA nanodevice, termed intelligent layered nanoflare, which integrates DNA computing at the nanoscale, via the self-assembly of DNA flares on a single gold nanoparticle. As a "lab-on-a-nanoparticle", the intelligent layered nanoflare could be engineered to perform a variety of Boolean logic gate operations, including three basic logic gates, one three-input AND gate, and two complex logic operations, in a digital non-leaky way. In addition, the layered nanoflare can serve as a programmable strategy to sequentially tune the size of nanoparticles, as well as a new fingerprint spectrum technique for intelligent multiplex biosensing. More importantly, the nanoflare developed here can also act as a single entity for intracellular DNA logic gate delivery, without the need of commercial transfection agents or other auxiliary carriers. By incorporating DNA circuits on nanoparticles, the presented layered nanoflare will broaden the applications of DNA circuits in biological systems, and facilitate the development of DNA nanotechnology.
    Nanoscale 06/2014; · 6.73 Impact Factor
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    ABSTRACT: The APTES/DMOAP were immobilized on the glass slide. The low density of PDGF-BB aptamer was coated through glutaraldehyde crosslinking and had little effect on disrupting the ordered LC alignment. In the presence of PDGF-BB, the structure of the aptamer had changed and made a visible optical change via the huge steric effect of the PDGF-BB on disrupting the LC alignment.
    Chinese Journal of Analytical Chemistry 05/2014; 42(5):629–635. · 0.79 Impact Factor
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    ABSTRACT: The water-soluble CP was conjugated with a rhodamine spirolactam for the first time to develop a new FRET-based ratiometric fluorescence sensing platform () for intracellular metal-ion probing. exhibits excellent water-solubility with two well-resolved emission peaks, which benefit ratiometric intracellular imaging applications.
    Chemical Communications 01/2014; · 6.38 Impact Factor
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    ABSTRACT: In this work, by combining the enzymatic recycling reaction with the DNA functionalized gold nanoparticles (AuNPs)-based signal amplification, we have developed an electrochemical biosensor for label-free detection of DNA with high sensitivity and selectivity. In the new designed biosensor, a hairpin-structured probe HP was designed to hybridize with target DNA first, and an exonuclease ExoIII was chosen for the homogeneous enzymatic cleaving amplification. The hybridization of target DNA with the probe HP induced the partial cleavage of the probe HP by ExoIII to release the enzymatic products. The enzymatic products could then hybridize with the hairpin-structured capture probe CP modified on the electrode surface. Finally, DNA functionalized AuNPs was further employed to amplify the detection signal. Due to the capture of abundant methylene blue (MB) molecules by both the multiple DNAs modified on AuNPs surface and the hybridization product of capture DNA and enzymatic products, the designed biosensor achieved a high sensitivity for target DNA, and a detection limit of 0.6pM was obtained. Due to the employment of two hairpin-structured probes, HP and CP, the proposed biosensor also exhibited high selectivity to target DNA. Moreover, since ExoIII does not require specific recognition sequences, the proposed biosensor might provide a universal design strategy to construct DNA biosensor which can be applied in various biological and medical samples.
    Biosensors & Bioelectronics 11/2013; 54C:442-447. · 6.45 Impact Factor
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    ABSTRACT: Aptamer-based fluorescence anisotropy (FA) assays have attracted great interest in recent years. However, a key factor that determines FA value is molar mass, thus limiting the utility of this assay for the detection of small molecules. To solve this problem, streptavidin, as a molar mass amplifier, was used in a hybridization chain reaction (HCR) to construct a target-triggered cyclic assembly of DNA-protein hybrid nanowires for highly sensitive detection of small molecules by fluorescence anisotropy. In this assay, one blocking DNA strand is released by target-aptamer recognition. The DNA then serves as an initiator to trigger enzyme-free autonomous cross-opening of hairpin probes via HCR to form a DNA nanowire for further assembly of streptavidin. Using adenosine triphosphate (ATP) as the small molecule target, this novel dual-amplified, aptamer-based FA assay affords high sensitivity with a detection limit of 100 nM. This LOD is much lower than that of the disassembly approach without HCR amplification or the assembly strategy without streptavidin. In contrast to the previous turn-off disassembly approaches based on nonspecific interactions between the aptamer probe and amplification moieties, the proposed aptamer-based FA assay method exhibits a turn-on response to ATP, which can increase sensing reliability and reduce the risk of false hits. Moreover, because of its resistance to environmental interferences, this FA assay has been successfully applied for direct detection of 0.5 μM ATP in complex biological samples, including cell media, human urine, and human serum, demonstrating its practicality in real complex biological systems.
    Analytical Chemistry 10/2013; · 5.82 Impact Factor
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    ABSTRACT: In this work, we developed a multiple amplification-based electrochemical sensor for ultra-sensitive detection of nucleic acids, using a disease-related sequence of p53 gene as the model target. A capture probe (CP) with hairpin structure is immobilized on the electrode surface via thiol-gold bonding, while its stem is designed to contain a restriction site for EcoRI. In the absence of target DNA, the probe keeps a closed conformation and forms a cleavable region. After treated with EcoRI, the target binding portion (loop) plus the biotin tag can be peeled off, suppressing the background current. In contrast, the CP is opened by the target hybridization, deforming the restriction site and forcing the biotin tag away from the electrode. Based on the biotin-streptavidin complexation, gold nanoparticles (GNPs) modified with a large number of ferrocene-signaling probes (Fc-SPs) are captured by the resulting interface, producing an amplified electrochemical signal due to the GNP-based enrichment of redox-active moieties. Furthermore, Fc tags can be dragged in close proximity to the electrode surface via hybridization between the signaling probes and the CP residues after EcoRI treatment, facilitating interfacial electron transfer and further enhancing the signal. With combination of these factors, the present system is demonstrated to achieve an ultrahigh sensitivity of zeptomole level and a wide dynamic response range of over seven orders of magnitudes.
    Analytical Chemistry 08/2013; · 5.82 Impact Factor
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    ABSTRACT: H2S is the third endogenously generated gaseous signaling compound, and has also been known to involve in a variety of physiological processes. To better understand its physiological and pathological functions, efficient methods for direct monitoring of H2S in living systems are desired. Although quite a few one photon fluorescence probes have been reported for H2S, two-photon (TP) probes are more favorable for intracellular imaging. In this work, by employing a Donor--Acceptor-structured naphthalene derivative as the two-photon fluorophore and an azide group as the recognition unit, we reported a new two-photon bio-imaging probe 6-(benzo[d]thiazol-2-yl)-2-azidonaphthalene (NHS1) for H2S with improved sensitivity. The probe shows very low background fluorescence in the absence of H2S. In the presence of H2S, however, a significant enhancement for both one photon and TP excited fluorescence were observed, resulting in a high sensitivity to H2S in aqueous solutions with a detection limit of 20 nM observed, much lower than previously reported TP probe. The probe also exhibits a wide linear response concentration range (0-5 μM)to H2S with high selectivity. All these features are favorable for direct monitoring of H2S in complex biological samples. It was then applied for direct TP imaging of H2S in living cells with satisfactory sensitivity, demonstrating its value of practical application in biological systems.
    Analytical Chemistry 07/2013; · 5.82 Impact Factor
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    ABSTRACT: A novel fluorometric assay method based on target-induced signal on was developed for acetylcholinesterase (AChE) inhibitor with obviously improved detection sensitivity. In this method, the AChE molecules catalyzed the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, which in turn can specifically react with fluorescent squaraine derivative, a specific chemodosimeter for thiol-containing compounds, resulting in fluorescence quenching and offering a low fluorometic background for the further detection of AChE inhibitor. In the presence of AChE inhibitor, the catalytic hydrolysis of ATCl is blocked, and then the squaraine derivative remains intact and shows signal-on fluorescence. The amount of the remaining fluorescent squaraine derivative is positively correlated with that of the AChE inhibitor in solution. This new designed sensing system shows an obviously improved sensitivity towards target with a detection limit of 5 pg mL-1 (0.018 nM) for the AChE inhibitor, compared favorably with previously reported fluorometric methods. To our best knowledge, this new method is the first example of fluorometric enzymatic assay for AChE inhibitors based on such a signal-on principle and using a specific reaction, which has potential to offer an effective strategy for the detection of AChE inhibitors.
    Analytical Chemistry 04/2013; · 5.82 Impact Factor
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    ABSTRACT: Gold nanoparticles-based colorimetric assay possesses several unique advantages, and has been applied for a wide range of targets, varying from nucleic acids to different metal ions. However, due to the lack of proper coordinating ligand, gold nanoparticles-based colorimetric sensing system for Au(3+) has not been developed so far. It is well-known that Au(3+) could induce the oxidation transition of thiol compounds to disulfide compounds. In this article, for the first time we converted such thiol masking reaction into colorimetric sensing system for label-free detection of Au(3+) via a target-controlled aggregation of nanoparticles strategy. In the new proposed sensing system, fluorosurfactant-capped gold nanoparticles were chosen as signal reporter units, while an Au(3+)-triggered oxidation of cysteine (Cys), which inhibited the aggregation of gold nanoparticles, acted as the recognition unit. By varying the amount of Cys, a tunable response range accompanied with different windows of color change could be obtained for Au(3+), illustrating the universality of the sensing system for Au(3+) samples with different sensitivity requirements. Under optimized condition, the proposed sensing system exhibits a high sensitivity towards Au(3+) with a detection limit of 50nM, which is lower than previously reported spectroscopic methods. It has also been applied for detection of Au(3+) in practical water samples with satisfactory result.
    Biosensors & Bioelectronics 04/2013; 48C:1-5. · 6.45 Impact Factor
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    ABSTRACT: A new and facile strategy using double-stranded DNA-copper nanoparticles (dsDNA-Cu NPs) as fluorescence reporters for the highly sensitive and selective detection of l-histidine was demonstrated. In the dsDNA-Cu NPs-based sensing system, the fluorescence was quenched considerably upon the addition of l-histidine. Under the optimized experimental conditions, the probe exhibits excellent performance (e.g., a satisfactory detection limit of 5μM and high specificity). Our in situ method requires no covalent attachment of DNA to a fluorophore, which could significantly reduce the cost and simplify the procedure for l-histidine detection. Moreover, the proposed sensing system could be applicable for the detection of target biomolecule in complex biological samples. These striking properties make it an attractive platform for the direct detection of l-histidine.
    Talanta 03/2013; 107C:402-407. · 3.50 Impact Factor
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    ABSTRACT: A novel template-dependent extension based isothermal amplification (TEIA) system with high single-base discrimination capability is developed, where the interference caused by non-specific reaction in isothermal strand displacement amplification (SDA) technique is substantially avoided via using a functionalized template probe, showing potential value in the development and application of SDA based detection devices.
    Chemical Communications 02/2013; 49(24):2448-50. · 6.38 Impact Factor
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    ABSTRACT: The self-assembly of cyclodextrin (CD) functionalized graphene (GR) and adamantane-modified horseradish peroxidase (HRP-ADA) by host-guest supramolecular interaction into novel nanostructures in aqueous solution is reported in the present study. Electrochemical impedance spectroscopy and cyclic voltammetry were applied to characterize the self-assembly process and study the electrochemical behaviors of the immobilized proteins. UV-vis spectra indicated that the native structure of HRP was maintained after the assembly, implying good biocompatibility of CD-functionalized GR (CD-GR). Furthermore, the HRP-ADA/CD-GR composites were utilized for the fabrication of enzyme electrodes (HRP-ADA/CD-GR electrodes). The proposed biosensor showed good reproducibility and high sensitivity to H2O2 with the detection limit of 0.1μM. In the range of 0.7-35μM, the catalytic reduction current of H2O2 was proportional to H2O2 concentration.
    Biosensors & Bioelectronics 02/2013; 45C:102-107. · 6.45 Impact Factor
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    ABSTRACT: The development of autonomous DNA machines and their use for specific sensing purpose have recently attracted considerable research attention. In existing autonomous machines, the target recognition process and signal transduction are separated from each other. This results in misunderstanding of the operation behavior, and the assay capability is compromised when serving as a sensing tool. In this communication, the integrated signal transduction-based autonomous aptameric machine, in which the recognition element and signal reporters are integrated into a DNA strand, is developed. This new machine can execute the in situ amplification of target binding-induced signal. The authentic operation behavior of autonomous DNA machine is discovered: the machine's products directly hybridize to the "track" rather than to the signaling probes. Along this line, the machine is employed to detect the cocaine in a more straightforward fashion, and improved assay characteristics (for example, the dynamic response range is widened by more than 500-fold) are achieved. Our efforts not only clarify the concept described in traditional autonomous DNA machines but also have made technological advancements that are expected to be especially valuable in designing nucleic acid-based machines employed in basic research and medical diagnosis.
    Biosensors & Bioelectronics 01/2013; 44C:95-100. · 6.45 Impact Factor
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    ABSTRACT: In this study, to surmount the dependence of LC biosensor on the geometrical dimension of responsing molecules, we designed a new label-free LC biosensor for the highly selective and sensitive detection of heavy metal ions. This strategy takes use of the target-induced DNA conformational change to enhance the disruption of target molecules for the orientation of LC leading to an amplified optical signal. The Hg2+ ion, which possesses a unique property to bind specifically to two DNA thymine base (T), is used as a model heavy metal ions. In the presence of Hg2+, the specific oligonucleotide probes form a conformational reorganization of the oligonucleotide probes from hairpin structure to duplex-like complexes. The duplex-like complexes are then bound on the TEA/DMOP-coated substrate modified with capture probes, which can greatly distort the orientational profile of LC, making the optical image of LC cell birefringent as a result. The optical signal of LC sensor has a visible change at the Hg2+ concentration low to 0.1 nM, showing good detection sensitivity. The cost-effective LC sensing method can translate the concentration signal of heavy metal ions in solution into the presence of DNA duplexes, and is expected to be a sensitive detection platform for heavy metal ions and other small molecule monitor.
    Analytical Chemistry 12/2012; · 5.82 Impact Factor
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    ABSTRACT: The generation of 8-oxo-7,8-dihydroguanine (8-oxoG) in DNA is a common type of DNA damage after exposure to reactive oxygen species or drugs. Human 8-oxoG DNA glycosylase/AP lyase (hOGG1) is a kind of base excision repair enzyme specifically used to repair the base excision of 8-oxoG. In this paper, we develop a novel, simple and sensitive strategy for the detection of hOGG1 activity based on the self-assembly of the active HRP-mimicking DNAzyme coupled with lambda exonuclease (λ exo) cleavage. We designed two DNA oligonucleotides that are fully complementary to each other. One is modified with 8-oxoG, the other contains the G-quadruplex DNAzyme sequence. The two single-stranded DNA (ssDNA) firstly hybridize to form a DNA duplex containing an 8-oxoG. In the presence of hOGG1, the formed DNA duplex is selectively cleaved at the 8-oxoG site, yielding a new DNA duplex with a recessed 5′-phosphate terminus. Upon treatment with λ exo, the 5′-phosphoryl ssDNA of the new DNA duplex is digested by λ exo, releasing the G-quadruplex DNAzyme sequence. After addition of hemin, the G-quadruplex–hemin complex is used as a peroxidase-mimicking DNAzyme, catalyzing H2O2-mediated oxidation of 2,2′-azinobis(3-ethylbenzothiozoline)-6-sulfonic acid (ABTS2−) to generate a colorimetric signal. The activity of hOGG1 is directly related to UV/Vis absorption intensity. The results revealed that the method allowed a sensitive quantitative assay of the hOGG1 concentration with a wide range from 0.05–32 U mL−1 and a low detection limit of 0.01 U mL−1.
    Analytical methods 12/2012; 5(1):164-168. · 1.86 Impact Factor
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    ABSTRACT: FRET strategy has been widely applied in designing ratiometric probes for bio-imaging applications. Unfortunately, for FRET systems, sufficiently large spectral overlap is necessary between the donor emission and the acceptor absorption, which would limit the resolution of double-channel images. Through-bond energy transfer (TBET) system does not need spectral overlap between donor and acceptor, and could afford large wavelength difference between the two emissions with improved imaging resolution and higher energy transfer efficiency than that of classic FRET system. It seems to be more favorable for designing ratiometric probes for bio-imaging applications. In this paper, we have designed and synthesized a coumarin-rhodamine (CR) TBET system, and demonstrated that TBET is a convenient strategy to design efficient ratiometric fluorescent bio-imaging probe for metal ions. Such TBET strategy is also universal, since no spectral overlap between the donor and the acceptor is necessary, and much more dye pairs than that of FRET could be chosen for probe design. As a proof-of-concept, Hg2+ was chosen as a model metal ion. By combining TBET strategy with dual-switch design, the proposed sensing platform shows two well-separated emission peaks with a wavelength difference of 110 nm, high energy transfer efficiency and a large signal-to-background ratio, which affords a high sensitivity for the probe with a detection limit of 7 nM for Hg2+. Moreover, by employing an Hg2+-promoted desulfurization reaction as recognition unit, the probe also shows a high selectivity to Hg2+. All these unique features make it particularly favorable for ratiometric Hg2+ sensing and bioimaging applications. It has been preliminarily used for ratiometric image of Hg2+ in living cells and practical detection of Hg2+ in river water samples with satisfying results.
    Analytical Chemistry 11/2012; · 5.82 Impact Factor
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    ABSTRACT: The double-strand DNA (dsDNA) can act as an efficient template for the formation of copper nanoparticles (Cu NPs) with high fluorescence, whereas the single-strand DNA (ssDNA) cannot support the formation of Cu NPs. This difference in fluorescent signal generation can be used for the detection of nuclease cleavage activity. Thus, a label-free strategy for sensitive detection of nuclease has been developed. The sensor contains a complete complementary dsDNA which acts as a template for the formation of Cu NPs and generation of fluorescence signal. The enzyme S1 nuclease was taken as the model analyte. Upon addition of S1 nuclease into the sensing system, the DNA was cleaved into fragments, preventing the formation of the Cu NPs and resulting in low fluorescence. In order to achieve the system's best sensing performance, a series of experimental conditions were optimized. Under the optimized experimental conditions, the sensor exhibits excellent performance (e.g., a detection limit of 0.3UmL(-1) with high selectivity). This possibly makes it an attractive platform for the detection of S1 nuclease and other biomolecules.
    Biosensors & Bioelectronics 10/2012; 42C:31-35. · 6.45 Impact Factor
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    ABSTRACT: In this work, we developed an electrochemical detection method based on allele-specific polymerase chain reaction (AS-PCR) and surface hybridization assay technique for the point mutation detection. A high-fidelity Vent(R)™(exo(-)) DNA polymerase, which eliminated the 3'→5' proofreading exonuclease activity by genetical engineering, was used to discriminate and extend the detection probe that perfectly matched with mutant target DNA and generate a redox-active DNA replica which folded into a molecular beacon structure by intramolecular hybridization. After hybridized with capture probe modified on gold electrode by self-assembly reaction, the redox tags can be closed to electrode, resulting in a substantial current with the maximized sensitivity for point mutation analysis. However, when there is an allele mismatch in the wild target DNA, and so no the redox-active replica DNA can be obtained. In this case, no remarkable current signal can be trigged. The proposed approach has been successfully implemented for the identification of single base mutation at the -28 position in human β-globin gene with a detection limit of 0.5fM, demonstrating that this method provides a highly specific, sensitive and cost-efficient approach for point mutation detection.
    Biosensors & Bioelectronics 10/2012; 42C:526-531. · 6.45 Impact Factor
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    ABSTRACT: A simple and universal quencher-free molecular beacon (MB) with low background fluorescence is developed based on an intermolecular G-quadruplex signaling probe. Unlike previous fluorescent MB strategies, it can function without any fluorophore and quencher modifications on its hairpin sequence.
    Chemical Communications 09/2012; 48(87):10760-2. · 6.38 Impact Factor

Publication Stats

3k Citations
971.75 Total Impact Points

Institutions

  • 1996–2014
    • Hunan University
      • • College of Chemistry and Chemical Engineering
      • • Department of Chemical Engineering
      Ch’ang-sha-shih, Hunan, China
  • 2013
    • Qufu Normal University
      Küfow, Shandong Sheng, China
  • 2012–2013
    • Jiangxi Agricultural University
      Jiangxi, Gansu Sheng, China
    • Guilin University of Electronic Technology
      Ling-ch’uan, Guangxi Zhuangzu Zizhiqu, China
  • 2010
    • Changsha University of Science and Technology
      Ch’ang-sha-shih, Hunan, China
    • Jiangsu University
      • School of the Environment
      Chenkiang, Jiangsu Sheng, China
    • Tianjin University of Traditional Chinese Medicine
      T’ien-ching-shih, Tianjin Shi, China
    • Zhengzhou Tobacco Institute
      Chen-chu-shan, Jiangxi Sheng, China
  • 2004–2010
    • Hunan University of Science and Technology
      Siangtan, Hunan, China
  • 2009
    • Central South University of Forestry and Technology
      Ch’ang-sha-shih, Hunan, China
    • Dalian University of Technology
      • State Key Laboratory of Fine Chemicals
      Dalian, Liaoning, China
    • China Jiliang University
      Hang-hsien, Zhejiang Sheng, China
  • 2008
    • Ningxia University
      Ning-hsia, Ningxia Huizu Zizhiqu, China
  • 2006
    • Kwansei Gakuin University
      Nishinomiya, Hyōgo, Japan
  • 2000
    • Shanghai Teachers University
      Shanghai, Shanghai Shi, China
  • 1999
    • Hunan Institute of Engineering
      Siangtan, Hunan, China
  • 1998
    • Hunan University of Technology
      Chu-chou-shih, Hunan, China
  • 1997
    • Changsha Railway University
      Ch’ang-sha-shih, Hunan, China