Ionel Marius Stoian

Stefan cel Mare University of Suceava, Suceava, Suceava, Romania

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Publications (7)9.5 Total impact

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    ABSTRACT: Aim. To establish allele frequencies and genetic parameters for 5 new European Standard Set short tandem repeat (STR) loci in the population of Romania and to compare them with those in other populations. Methods. DNA was isolated using QIAamp 96 DNA Swab BioRobot Kit and Chelex 100 methods. Polymerase chain reaction amplification was done using Investigator ESSplexPlus Kit (D1S1656, D2S441, D2S1338, D3S1358, D8S1179, D10S1248, D12S391, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, TH01, and vWA). For DNA typing, Applied Biosystems 3500/3500xL Genetic Analyzer was used. Statistical analysis was done using Powerstats, GDA, and Arlequin software. Results. Power of discrimination and polymorphism information content was highest for two new ESS loci, D1S1656 and D12S391. Comparison of allele frequencies for 5 new ESS loci in Romanian population with previously published population data showed significant differences for all compared populations, with the exception of Hungary. Geographically more distant populations, such as Spain, Sweden, United Kingdom, Germany, and Portugal differed more than closer populations. Conclusion. New ESS STR loci are very useful for the analysis of forensic samples (persons or traces) due to their characteristics (shortness and high polymorphism). In comparisons with other common STR markers, they have a higher power of discrimination and also higher polymorphism information content, and could be used in any national DNA database.
    Croatian Medical Journal 06/2013; 54(3):232-7. · 1.25 Impact Factor
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    ABSTRACT: Haplotypes and allele frequencies for 17 STR loci included in AmpFlSTR YFiler kit (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATAH4, DYS437, DYS438 and DYS448) were determined in a sample of 122 unrelated males living in the South-East Romania. Genetic distances had been calculated and dendrograms had been generated for South-East Romanian population and other eighteen surrounding populations.
    Legal Medicine 09/2010; 12(5):259-64. · 1.08 Impact Factor
  • Florin Stanciu, Oana Raluca Popescu, Ionel Marius Stoian
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    ABSTRACT: Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 1321 unrelated individuals living in the region of Moldavia (NE Romania). No deviations from Hardy-Weinberg equilibrium were observed (only after applying the Bonferroni correction in the cases of D2S1338). Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.
    Forensic science international. Genetics 12/2009; 4(1):e39-40. · 2.42 Impact Factor
  • Source
    Florin Stanciu, Ionel Marius Stoian, Oana Raluca Popescu
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    ABSTRACT: To determine allele frequencies' distribution for 15 short tandem repeat (STR) loci in a population sample of 1910 unrelated individuals from the region of Wallachia, South Romania. DNA was isolated using Chelex 100 method and an adapted version of AGOWA mag DNA Isolation Kit Sputum. Polymerase chain reaction amplification was done using AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA). For DNA typing, ABI PRISM 3100 Genetic Analyzer was used. Genetic parameters of forensic interest were calculated and comparisons with geographically close populations were performed. With the exception of vWA locus (P=0.001), no other significant deviations from Hardy-Weinberg expectations were found. Single locus comparisons with data on geographically close populations showed significant differences between the population of Wallachia and the population of Bucharest area, Greece, Turkey, Italy, Hungary, Belarus, and Poland, but no differences were found from the population from Croatia and Serbia. According to 15 analyzed STR loci, the population of Wallachia region was found to be genetically more similar to Slavic populations of Croatia and Serbia than to other surrounding populations.
    Croatian Medical Journal 07/2009; 50(3):321-5. · 1.25 Impact Factor
  • Florin Stanciu, Oana Raluca Popescu, Ionel Marius Stoian
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    ABSTRACT: Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 569 unrelated individuals living in the region of Dobruja (SE Romania). No deviations from Hardy-Weinberg equilibrium were observed. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.
    Forensic science international. Genetics 04/2009; 3(2):146-7. · 2.42 Impact Factor
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    Florin Stanciu, Ionel Marius Stoian, Oana Raluca Popescu
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    ABSTRACT: Nowadays, automation is crucial in databasing laboratories especially for countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to supply genetic profiles for Romanian National DNA Database, we evaluated the Freedom Evo 150 liquid handling platform with AGOWA sep9600 magnetic separator in different running conditions and workflows, for reaching the most efficient ones. We compared the results with those obtained from manual DNA isolation with Chelex resin and paramagnetic particles. For this we used a total of 6246 biological reference samples (5220 - automated processed and 1026 - manually processed), amplified using AmpFlstr Identifiler Kit and analyzed by capillary electrophoresis using ABI Prism 3100 Genetic Analyzer.
    Romanian Journal of Legal Medicine - ROM J LEG MED. 01/2009; 17(1):51-58.
  • Florin Stanciu, Ionel Marius Stoian, Oana Raluca Popescu
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    ABSTRACT: Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 1977 unrelated individuals living in the region of Transylvania (NW Romania). No deviations from Hardy-Weinberg equilibrium were observed with the exception of D3S1358 and D16S539. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.
    Legal Medicine 12/2008; 11(1):48-50. · 1.08 Impact Factor