Publications (29)83.82 Total impact
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Article: Highly efficient enzymatic preparation of c-di-AMP using the diadenylate cyclase DisA from Bacillus thuringiensis.
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ABSTRACT: Cyclic 3',5'-diadenosine monophosphate (c-di-AMP) is a newly recognized bacterial nucleotide second messenger molecule. In addition, it has been shown to be a potential vaccine adjuvant. Although multiple methods are available for c-di-AMP synthesis, the yields are low and the purification procedures are laborious. Here, we report an enzymatic method for more efficient and economical c-di-AMP synthesis using a diadenylate cyclase DisA from Bacillus thuringiensis BMB 171 (btDisA). After overexpression and purification of btDisA, the enzyme-catalyzed reaction conditions were further investigated. Under the optimum conditions, in which 100mM CHES (pH 9.5) containing 2μM btDisA, 10mM ATP, and 10mM MgCl2 was incubated at 50°C for 4h, a high conversion rate of c-di-AMP was obtained. Coupling this process with HPLC purification and lyophilization yielded 100mg of highly pure c-di-AMP that was harvested in white powder form from a 50mL enzyme-catalyzed reaction system. The protocol is not only directly applicable for preparing abundant amounts of c-di-AMP for extensive biochemical and immunological use, but can also be scaled up to meet the requirements for medical applications.Enzyme and microbial technology. 05/2013; 52(6-7):319-24. -
Article: The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.
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ABSTRACT: Bacillus thuringiensis is a well known entomopathogenic bacterium used worldwide as an environmentally compatible biopesticide. During sporulation, B. thuringiensis accumulates a large number of parasporal crystals consisting of insecticidal crystal proteins (ICPs) that can account for nearly 20~30% of the cell dry weight. However, the metabolic regulation mechanisms of ICP synthesis remain to be elucidated. In this study, the combined efforts in transcriptomics and proteomics mainly uncovered the following 6 metabolic regulation mechanisms: 1) proteases and the amino acid metabolism (particularly, the branched-chain amino acids) became more active during sporulation; 2) stored poly-β-hydroxybutyrate and acetoin, together with some low-quality substances provided considerable carbon and energy sources for sporulation and parasporal crystal formation; 3) the pentose phosphate shunt demonstrated an interesting regulation mechanism involving gluconate when CT-43 cells were grown in GYS medium; 4) the tricarboxylic acid cycle was significantly modified during sporulation; 5) an obvious increase in the quantitative levels of enzymes and cytochromes involved in energy production via the electron transport system was observed; 6) most F0F1-ATPase subunits were remarkably up-regulated during sporulation. This study, for the first time, systematically reveals the metabolic regulation mechanisms involved in the supply of amino acids, carbon substances, and energy for B. thuringiensis spore and parasporal crystal formation at both the transcriptional and translational levels.Molecular & Cellular Proteomics 02/2013; · 7.40 Impact Factor -
Article: CotA, a Multicopper Oxidase from Bacillus pumilus WH4, Exhibits Manganese-Oxidase Activity.
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ABSTRACT: Multicopper oxidases (MCOs) are a family of enzymes that use copper ions as cofactors to oxidize various substrates. Previous research has demonstrated that several MCOs such as MnxG, MofA and MoxA can act as putative Mn(II) oxidases. Meanwhile, the endospore coat protein CotA from Bacillus species has been confirmed as a typical MCO. To study the relationship between CotA and the Mn(II) oxidation, the cotA gene from a highly active Mn(II)-oxidizing strain Bacillus pumilus WH4 was cloned and overexpressed in Escherichia coli strain M15. The purified CotA contained approximately four copper atoms per molecule and showed spectroscopic properties typical of blue copper oxidases. Importantly, apart from the laccase activities, the CotA also displayed substantial Mn(II)-oxidase activities both in liquid culture system and native polyacrylamide gel electrophoresis. The optimum Mn(II) oxidase activity was obtained at 53°C in HEPES buffer (pH 8.0) supplemented with 0.8 mM CuCl2. Besides, the addition of o-phenanthroline and EDTA both led to a complete suppression of Mn(II)-oxidizing activity. The specific activity of purified CotA towards Mn(II) was 0.27 U/mg. The Km, Vmax and kcat values towards Mn(II) were 14.85±1.17 mM, 3.01×10(-6)±0.21 M·min(-1) and 0.32±0.02 s(-1), respectively. Moreover, the Mn(II)-oxidizing activity of the recombinant E. coli strain M15-pQE-cotA was significantly increased when cultured both in Mn-containing K liquid medium and on agar plates. After 7-day liquid cultivation, M15-pQE-cotA resulted in 18.2% removal of Mn(II) from the medium. Furthermore, the biogenic Mn oxides were clearly observed on the cell surfaces of M15-pQE-cotA by scanning electron microscopy. To our knowledge, this is the first report that provides the direct observation of Mn(II) oxidation with the heterologously expressed protein CotA, Therefore, this novel finding not only establishes the foundation for in-depth study of Mn(II) oxidation mechanisms, but also offers a potential biocatalyst for Mn(II) removal.PLoS ONE 01/2013; 8(4):e60573. · 4.09 Impact Factor -
Article: Proteomic analysis of Bacillus thuringiensis ΔphaC mutant BMB171/PHB(-1) reveals that the PHB synthetic pathway warrants normal carbon metabolism.
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ABSTRACT: A phaC knockout mutant from Bacillus thuringiensis (Bt) strain BMB171, named BMB171/PHB(-1), was constructed. A physiological and metabolic investigation and a proteomic analysis were conducted for both ΔphaC mutant and its parent strain. Grown in peptone medium with 5 gram glucose per liter as sole carbon source, BMB171/PHB(-1) produced various organic acids. Here the excreted pyruvate, citrate, lactate, acetate and glutamate were quantitatively analyzed. Deletion of phaC gene from the BMB171 strain resulted in 1) growth delay; 2) higher consumption of dioxigen but lower cell yield; 3) stagnation of pH movement; 4) overproduction of organic acids; 5) rapid descent of cell density in the stationary phase; and 6) a sporulation-deficient phenotype. Our proteomic study with qPCR reconfirmation reveals that the absence of PhaC led to a metabolic turmoil which showed repressed glycolysis, and over-expressed TCA cycle, various futile pathways and amino acid synthesis during vegetative growth. It is thus thought that B. thuringiensis BMB171 effectively regulated its carbon metabolism upon the presence of the functional PHB synthetic pathway. The presence of this pathway warrants a PHB-producing bacterium better surviving under different environmental conditions.Journal of proteomics 06/2012; 75(17):5176-88. · 5.07 Impact Factor -
Article: Characteristics and flocculating mechanism of a novel bioflocculant HBF-3 produced by deep-sea bacterium mutant Halomonas sp. V3a’
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ABSTRACT: A novel bioflocculant HBF-3 produced by deep-sea bacterium mutant Halomonas sp. V3a’ was investigated with regard to its flocculating characteristics and mechanism. 4.0mgl−1 HBF-3 showed the maximum flocculating activity of 96.9% in 5.0gl−1 Kaolin suspension containing 11.25mM CaCl2, and that its flocculating activity was more than 90% within 5–40°C and over 80% in a wide pH range (3.0–11.0). Chemical analyses indicated that the biopolymer HBF-3 was mainly a polysaccharide, including neutral sugar residues (20.6%), uronic acid residues (7.6%), amino sugar residues (1.6%) and sulfate groups (5.3%). Fourier transform infrared (FTIR) spectrum showed the presence of carboxyl and hydroxyl groups in HBF-3 molecular. The average molecular mass of HBF-3, as determined by gel filtration chromatography (GFC), was approximately 590kDa. Flocculation of Kaolin suspension with HBF-3 acted as a model to explore the flocculating mechanism in which bridging mediated by Ca2+ was proposed as the primary action based upon the experimental observations. KeywordsDeep-sea bacterium- Halomonas sp. V3a’-Bioflocculant-Flocculating characteristics-Flocculating mechanismWorld Journal of Microbiology and Biotechnology 04/2012; 26(6):1135-1141. · 1.53 Impact Factor -
Article: Comparative proteomic analysis revealed metabolic changes and the translational regulation of Cry protein synthesis in Bacillus thuringiensis.
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ABSTRACT: B. thuringiensis strain YBT-1520 is highly toxic to some Lepidopteran pests and is used to control pests in southern part of China. The aim of this work is to uncover the metabolic changes associated with the ICP synthesis. A comparative proteomic analysis on the strain was performed with 2-DE and MALDI-TOF-MS methods with the bacterial culture grown in a modified defined minimal medium. Transcriptional expression of some key enzymes for carbohydrate metabolism was also investigated with qPCR. Seventy-two proteins differentially expressed at least two folds were identified. Significant changes were observed in metabolisms when comparing stationary growth phase to mid-log phase. In late-stationary phase, five major phenomena were observed: 1) the glycolysis cycle was redirected into pentose phosphate pathway and ended in serine formation; 2) the tricarboxylic acid (TCA) cycle was suppressed; 3) poly(3-hydroxybutyrate) (PHB) was considered to be the important energy and carbon supply; 4) collectively increased proteases suggested massive protein degradation to release amino acids, and 5) translation-related factors were up-regulated including EF-Tu, GroEL and GatB, while the negative regulator YfiA was down. The metabolic changes of bacterium during growth shift indicated that the cells acquired energy, amino acid precursors and translation-related factors for the ICP synthesis.Journal of proteomics 11/2011; 75(4):1235-46. · 5.07 Impact Factor -
Article: [Mechanism and application of molecular self-assembly in Sup35 prion domain of Saccharomyces cerevisiae].
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ABSTRACT: Sup35 in its native state is a translation termination factor in Saccharomyces cerevisiae. The prion domain of Sup35p can form amyloid-like proteinaceous fibrils in vitro and in vivo. Furthermore, the in-register cross beta-sheet structure of Sup35p amyloid fibrils is similar to those formed in other species. Therefore, studies on mechanism of Sup35p self-assembly can be an appropriate model to study protein misfolding-related diseases and prion biology. Because of its ability to self-assemble into nanowires, the prion domain of Sup35p has been widely used in biotechnology and nanotechnology.Sheng wu gong cheng xue bao = Chinese journal of biotechnology 10/2011; 27(10):1401-7. -
Article: [Analyze and compare metabolic pathways of Bacillus cereus group].
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ABSTRACT: A large number of data and information was obtained from genome sequencing and high-throughput genomic studies, use of the information to study metabolic networks become a new hotspot in biological research. This article compared different methods to reconstruct metabolic networks and analyzed the advantages and disadvantages of each methods, and then introduced some researches about carbohydrate metabolism pathways, amino acid metabolic pathways, and energy metabolism pathways of 9 strains of Bacillus cereus, 6 strains of B. anthracis,,6 strain of B. thuringiensis, and finds out their similarities and characteristics. These three strains have some necessary metabolic pathways, such as glycolysis, tri-carboxylic acid cycle, alanine metabolism, histidine metabolism, and energy metabolism, but they may have some specific pathways. B cereus has higher efficiency in utilizing monosaccharide, B. anthracis is rich in degradation and transport pathways of amino acids. A glutamate metabolic bypass way exists in B. thuringiensis. Analysis of metabolic pathways provides a new way to study and use food toxin, anthrax toxin, and insecticidal toxin of these strains in future.Hereditas (Beijing) 10/2011; 33(10):1057-66. -
Article: [Advances in clustered regularly interspaced short palindromic repeats--a review].
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ABSTRACT: The recently discovered Clustered Regularly Interspaced Short Palindromic Repeat (CRISPRs) can protect bacteria and archaea with adaptive and heritable defense systems against the invasion of phage- and plasmid- associated mobile genetic elements. Here, we review the structure, diversity, mechanism of interference and self versus non-self discrimination of CRISPR systems. We also discuss the potential applications of this novel interference system.ACTA MICROBIOLOGICA SINICA 08/2011; 51(8):1007-13. -
Article: Complete genome sequence of Bacillus thuringiensis subsp. chinensis strain CT-43.
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ABSTRACT: Bacillus thuringiensis has been widely used as an agricultural biopesticide for a long time. As a producing strain, B. thuringiensis subsp. chinensis strain CT-43 is highly toxic to lepidopterous and dipterous insects. It can form various parasporal crystals consisting of Cry1Aa3, Cry1Ba1, Cry1Ia14, Cry2Aa9, and Cry2Ab1. During fermentation, it simultaneously generates vegetative insecticidal protein Vip3Aa10 and the insecticidal nucleotide analogue thuringiensin. Here, we report the finished, annotated genome sequence of B. thuringiensis strain CT-43.Journal of bacteriology 07/2011; 193(13):3407-8. · 3.94 Impact Factor -
Article: Proteomic analysis reveals the strategies of Bacillus thuringiensis YBT-1520 for survival under long-term heat stress.
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ABSTRACT: Bacillus thuringiensis (Bt) has been widely used for 50 years as a safe biopesticide for controlling agricultural and sanitary insect pests because of its insecticidal crystal proteins. In this study a proteomic approach was used to investigate the responses and survival strategies of Bt YBT-1520 under a long-term heat stress condition (42°C). Heat stress mainly influenced the characteristics of YBT-1520 on four aspects: (i) the abilities to synthesise insecticidal crystal proteins and other potential pathogenic factors were almost lost, (ii) cell adhesion and motility were also lost, (iii) cell did not sporulate, (iv) cell kept accumulating poly(3-hydroxybutyrate) (PHB). Proteomic analyses to the physiological changes of the strain revealed three strategies of YBT-1520 for survival under long-term heat stress. The first strategy is to up-regulate enzymes (BDH1, GuaB and PepA) for long-term heat stress tolerance. The second one is to down-regulate metabolic enzymes to reduce metabolic burden. The third strategy is to increase the synthesis and accumulation of PHB. Under heat stress condition, the bacterium adjusted its metabolism by up-/down-regulation and continuous accumulation of PHB. These strategies would help cells to gain more tolerance to heat stress.Proteomics 07/2011; 11(13):2580-91. · 4.43 Impact Factor -
Article: Dispersive liquid-liquid microextraction based on solidification of floating organic droplet followed by high-performance liquid chromatography with ultraviolet detection and liquid chromatography-tandem mass spectrometry for the determination of triclosan and 2,4-dichlorophenol in water samples.
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ABSTRACT: A novel, simple and efficient dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) technique coupled with high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of triclosan and its degradation product 2,4-dichlorophenol in real water samples. The extraction solvent used in this work is of low density, low volatility, low toxicity and proper melting point around room temperature. The extractant droplets can be collected easily by solidifying it at a lower temperature. Parameters that affect the extraction efficiency, including type and volume of extraction solvent and dispersive solvent, salt effect, pH and extraction time, were investigated and optimized in a 5 mL sample system by HPLC-UV. Under the optimum conditions (extraction solvent: 12 μL of 1-dodecanol; dispersive solvent: 300 of μL acetonitrile; sample pH: 6.0; extraction time: 1 min), the limits of detection (LODs) of the pretreatment method combined with LC-MS/MS were in the range of 0.002-0.02 μg L(-1) which are lower than or comparable with other reported approaches applied to the determination of the same compounds. Wide linearities, good precisions and satisfactory relative recoveries were also obtained. The proposed technique was successfully applied to determine triclosan and 2,4-dichlorophenol in real water samples.Journal of chromatography. A 06/2011; 1218(25):3830-6. · 4.19 Impact Factor -
Article: Rational questing for potential novel inhibitors of FabK from Streptococcus pneumoniae by combining FMO calculation, CoMFA 3D-QSAR modeling and virtual screening.
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ABSTRACT: Enoyl-acyl carrier protein (ACP) reductase (ENR) is an attractive and potential target for developing selective antibacterial agents. Recent studies showed that FabK is the sole isoform of ENR in Streptococcus pneumoniae, and at the same time an X-ray crystallographic study of FabK from S. pneumoniae (SpFabK) was reported for the first time. Based on above information, the interaction mechanism and pair interaction energies between ligand and the active site of SpFabK were analyzed with the ab initio fragment molecular orbital (FMO) calculation based on the FlexX docking model at the FMO-RHF/6-31G* level. Subsequently, the first molecular docking-based 3D-QSAR model with comparative molecular field analysis (CoMFA) was established with cross-validated coefficients (q(2)) up to 0.511 and regression coefficients (r(2)) up to 0.986. Then integrating the 3D-QSAR CoMFA predicted model, molecular docking, and FMO pair interaction analysis structure-based virtual screening was performed, six novel and potential lead compounds were sorted out for further study.Journal of Molecular Modeling 06/2011; 17(6):1483-92. · 1.80 Impact Factor -
Article: [In situ enrichment and diversity analysis of polycyclic aromatic carbon degrading bacteria in the coastal seawater of Xiamen Island].
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ABSTRACT: The aim of this study is to assess the diversity of polycyclic aromatic carbon (PAH)-degrading bacteria in the coastal seawater of Xiamen Island. The phenanthrene-degrading bacteria were enriched by suspending phenanthrene-coated Polyvinylchloride (PVC) plates in the seawater close to Xiamen International Cruise Dock. PCR-DGGE and 16S rRNA gene clone library were used to analyze the bacteria colonizing on the PVC plates. Further, PAH-degrading bacteria were re-enriched in lab after the in situ enrichment and subjected to diversity analysis and bacterial isolation. After 6 days incubation, the genus Cycloclasticus was shown to be the dominant bacterium on the phenanthrene (Phe)-coated plates, which accounted for 50% of the total clones in 16S rRNA gene clone library. However, on the control plates without Phe-coating, bacteria of Rhodobacteraceae were the dominant member, which accounted for 47% of the total clones. PCR-DGGE results reconfirmed the genus Cycloclasticus as the dominant member on the Phe-coated plates. After re-enrichment with Phe in laboratory, 14 strains were isolated from the consortium, which contained a potential novel species of genus Novosphingobium, named strain B-14 and identified as a Phe degrader. However, the most predominant member Cycloclasticus can not be cultivated into pure culture. Genus Cycloclasticus is the most important PAH-degrading bacterium in the coast sea water of Xiamen Island. This is the first evidence to our knowledge about the in situ enrichment PAH-degrading bacterium in seawater.ACTA MICROBIOLOGICA SINICA 10/2010; 50(10):1392-8. -
Article: Prevalence and diversity of insertion sequences in the genome of Bacillus thuringiensis YBT-1520 and comparison with other Bacillus cereus group members.
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ABSTRACT: Members of the Bacillus cereus group are closely related bacteria that exhibit highly divergent pathogenic properties. Sequencing of Bacillus thuringiensis ssp. kurstaki strain YBT-1520 revealed an increased number of insertion sequences (ISs) compared with those of the published B. cereus group genomes. Although some of these ISs have been observed and summarized in B. thuringiensis previously, a genomic characterization of their content is required to reveal their distribution and evolution. The result shows that the larger number of transposase coding genes on YBT-1520 chromosome is mainly caused by the amplification of IS231C, IS232A and ISBth166. Some functional genes have been disrupted through the insertion of ISs, preferentially IS231C. By comparing the Southern hybridization profiles of different B. thuringiensis strains, the existence of ISBth166 was mainly found in serovar kurstaki and the recent expansion of IS231C between different kurstaki isolates was suggested. In addition to revealing the ISs profile in YBT-1520 as well as the comparison in the B. cereus group, this study will contribute to further comparative analyses of multiple B. thuringiensis strains aimed at understanding the IS-mediated genomic rearrangements among them.FEMS Microbiology Letters 09/2010; 310(1):9-16. · 2.04 Impact Factor -
Article: Complete genome sequence of Bacillus thuringiensis mutant strain BMB171.
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ABSTRACT: Bacillus thuringiensis has been widely used as a biopesticide for a long time. Here we report the finished and annotated genome sequence of B. thuringiensis mutant strain BMB171, an acrystalliferous mutant strain with a high transformation frequency obtained and stocked in our laboratory.Journal of bacteriology 08/2010; 192(15):4074-5. · 3.94 Impact Factor -
Article: Detection of Escherichia coli enoyl-ACP reductase using biarsenical-tetracysteine motif.
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ABSTRACT: Although the tetracysteine (TC) motif has been used as a tag, the binding stability between TC motif and biarsenical reagent against extreme conditions as well as its capacity as a quantitative tag remains not well developed. To reveal these problems, we chose enoyl-acyl carrier protein reductase (FabI), which was involved in the final step of elongation in the bacterial fatty acid biosynthesis, to be tagged by the TC motif. Taking enhanced green fluorescent protein (EGFP) tagged FabI as a control, we investigated the activities of various TC tagged FabIs (N-terminus, C-terminus, or both N- and C-terminus TC motif). The results showed that all the TC tagged FabIs had high enzyme activities while the EGFP tagged FabI exhaustively lost the activity. Beside this, the characteristics of the tag, including labeling stability against extreme conditions, capacity for quantitative analysis, and ability for in-cell labeling, were also investigated. We demonstrated for the first time that the binding between FlAsH reagent and TC motif was stable against high pressure, high field strength, high temperature, and ultrasound. Furthermore, we verified the potential of TC motif for quantitative analysis of target protein by different approaches, including SDS-PAGE, spectrofluorometry (SPF), and capillary zone electrophoresis (CZE).Bioconjugate Chemistry 07/2010; 21(7):1341-8. · 4.93 Impact Factor -
Article: An artificial light source influences mating and oviposition of black soldier flies, Hermetia illucens.
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ABSTRACT: Current methods for mass-rearing black soldier flies, Hermetia illucens (L.) (Diptera: Stratiomyidae), in the laboratory are dependent on sunlight. Quartz-iodine lamps and rare earth lamps were examined as artificial light sources for stimulating H. illucens to mate and lay eggs. Sunlight was used as the control. Adults in the quartz-iodine lamp treatment had a mating rate of 61% of those in the sunlight control. No mating occurred when the rare earth lamp was used as a substitute. Egg hatch for the quartz-iodine lamp and sunlight treatments occurred in approximately 4 days, and the hatch rate was similar between these two treatments. Larval and pupal development under these treatments required approximately 18 and 15 days at 28°C, respectively. Development of methods for mass rearing of H. illucens using artificial light will enable production of this fly throughout the year without investing in greenhouse space or requiring sunlight.Journal of Insect Science 01/2010; 10:202. · 0.95 Impact Factor -
Article: Novel enoyl-ACP reductase (FabI) potential inhibitors of Escherichia coli from Chinese medicine monomers.
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ABSTRACT: By structure-based virtual screening and experimental verification, two Chinese medicine monomers, luteolin and curcumin, had been proved to be uncompetitive inhibitors of enoyl-ACP reductase from Escherichia coli (EcFabI) with the inhibition constant (K(i)) of 7.1microM and 15.0microM, respectively. In particular, curcumin had apparent antibacterial activity against E. coli, and the minimum inhibition concentration (MIC(90)) was 73.7microg/mL. Importantly, fabI-overexpressing E. coli showed reduced susceptibility to the inhibitor compared with the wild-type strains, demonstrating that its antibacterial action is mediated by the inhibition of EcFabI.Bioorganic & medicinal chemistry letters 11/2009; 20(1):56-9. · 2.65 Impact Factor -
Article: Extending the working calibration ranges of four hexachlorocyclohexane isomers in gas chromatography-electron capture detector by radial basis function neural network.
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ABSTRACT: A radial basis function neural network (RBFNN) method was developed for the first time to model the nonlinear calibration curves of four hexachlorocyclohexane (HCH) isomers, aiming to extend their working calibration ranges in gas chromatography-electron capture detector (GC-ECD). Other 14 methods, including seven parametric curve fitting methods, two nonparametric curve fitting methods, and five other artificial neural network (ANN) methods, were also developed and compared. Only the RBFNN method, with logarithm-transform and normalization operation on the calibration data, was able to model the nonlinear calibration curves of the four HCH isomers adequately. The RBFNN method accurately predicted the concentrations of HCH isomers within and out of the linear ranges in certified test samples. Furthermore, no significant difference (p>0.05) was found between the results of HCH isomers concentrations in water samples calculated with RBFNN method and ordinary least squares (OLS) method (R(2)>0.9990). Conclusively, the working calibration ranges of the four HCH isomers were extended from 0.08-60 ng/ml to 0.08-1000 ng/ml without sacrificing accuracy and precision by means of RBFNN. The outstanding nonlinear modeling capability of RBFNN, along with its universal applicability to various problems as a "soft" modeling method, should make the method an appealing alternative to traditional modeling methods in the calibration analyses of various systems besides the GC-ECD.Talanta 09/2009; 79(3):916-25. · 3.79 Impact Factor
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Institutions
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2002–2013
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Huazhong Agricultural University
- • State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbial Pesticides
- • College of Life Science and Technology
Wuhan, Hubei, China
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