A Hossein-Nezhad

Publications (10)14.29 Total impact

• Article: Vitamin D receptor FokI genotype may modify the susceptibility to schizophrenia and bipolar mood disorder by regulation of dopamine D1 receptor gene expression.

  S Ahmadi, K Mirzaei, A Hossein-Nezhad, G Shariati
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  ABSTRACT: This study is designed to test association of FOKI polymorphism in Vitamin D receptor (VDR) gene and its potential effect on expression of dopamine D1 receptor in schizophrenia and bipolar mood disorder as well as in healthy individuals. In this case-control study 196 patient with schizophrenia, 119 patients with bipolar mood disorder and 192 healthy individuals as the control group were recruited. All psychiatric disorders were diagnosed according to DSM IV criteria. Healthy control group denied any family history of such disorders. FOKI was genotyped by means of PCR-RFLP method. The mRNA was extracted from the peripheral blood mononuclear cells (PBMC) and the cDNA was synthesized. Frequency of ff genotype was more common in patients with bipolar disorders compared to the healthy control group (Odds ratio=1.84, 95% CI; 0.81 to 4.17) with increased relative risk (Relative risk=1.31, CI 95%; 0.86 to 1.99). There were significant differences between relative expressions of dopamine D1 receptor gene in various genotypes. Our results indicated that the ff genotype was associated with lower expression of dopamine D1 receptor gene. VDR as a nuclear receptor may contribute to bipolar disorders via modification of the expression of the neurotransmitters receptor such as dopamine.
  Minerva medica 10/2012; 103(5):383-91. · 0.90 Impact Factor
• Article: Potential role of OPG/RANKL system and FokI genotypes in pathogenesis and clinical manifestations in multiple sclerosis.

  K Mirzaei, S Ahmadi, A Hossein-Nezhad, F Mokhtari
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  ABSTRACT: The OPG/RANKL has identified role in immune system via T-cell-activating cytokines. Considering that immune mechanisms play a key role in the pathogenesis of MS, OPG/RANKL might be importance in the underlying mechanism of the disease. The aim of this study is to measure plasma levels of OPG and RANKL as well as to analyze VDR FokI polymorphism (rs2228570) in MS patients and healthy individuals to detect any potential correlation. We included a total of 397 participants, 105 of them suffering from two different types of MS, namely relapsing and remitting and secondary progressive multiple sclerosis. VDR genotyping was performed using PCR-RFLP method. The results showed differences in the plasma levels of OPG and RANKL between patients and the healthy control group that were statistically significant. We found higher plasma levels of OPG and lower RANKL concentrations in RRMS patients in comparison with SPMS types of the disease. We detected higher plasma levels of OPG and lower levels of RANKL in subjects with F allele compared to those with f allele in healthy subjects. However, contradicting results were observed when patients with MS were analyzed. We detected lower plasma levels of OPG and higher RANKL concentrations in patients with F allele in comparison with those with f allele. This might define a role for FokI polymorphism and OPG/RANKL system in the pathogenesis and progression of multiple sclerosis with further practical applications.
  Minerva medica 08/2012; 103(4):313-21. · 0.90 Impact Factor
• Article: Cytokine profile, Foxp3 and nuclear factor-kB ligand levels in multiple sclerosis subtypes.

  S Alatab, Z Maghbooli, A Hossein-Nezhad, M Khosrofar, F Mokhtari
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  ABSTRACT: Patients with multiple sclerosis (MS) present with heterogeneous clinical courses. To elucidate whether different immunopathological mechanisms are involved in MS subgroups, we compared serum levels of TNF-α, IL-1β, hs-CRP, receptor activator of nuclear factor kappa-B ligand (RANKL) and peripheral blood foxp3 expression in clinical subtypes of MS (relapsing remitting: RR-MS; secondary progressive: SP-MS; primary progressive: PP-MS) and healthy subjects. In a case-control study, 72 healthy individuals and 72 age- and sex-matched multiple sclerotic patients (57% RR-MS, 18% SP- MS and 25% PP-MS) were evaluated. The age, gender distribution, and BMI of MS patients in these three sup-types were similar. The serum levels of TNF-α, IL-1β, and RANKL were measured by ELISA. hs-CRP was measured by imunoturbidimetric method. Peripheral blood mononuclear cells expression of Foxp3 was measured by real time PCR. A significant elevation of TNF-α, hs-CRP, IL-1β and RANKL and diminution of Foxp3 expression in MS patients compared to control was found (P<0.001). PP-MS had highest levels of TNF-α, IL-1β, CRP and RANKL, and lowest levels of foxp3, with difference in TNF-α reached significant level (P<0.01). RANKL and TNF-α showed a reverse (P<0.01) significant correlation with Foxp3 relative expression levels. Patients with early age onset (onset before 30 years) had significantly higher levels of hs-CRP compared to late age onset patients. These data demonstrate the presence of immunopathogenesis differences between relapsing and non-relapsing form and is also the first to stress a role for cytokine RANKL in MS patients.
  Minerva medica 12/2011; 102(6):461-8. · 0.90 Impact Factor
• Article: Genetic variation in macrophage migration inhibitory factor associated with gestational diabetes mellitus and metabolic syndrome.

  S Aslani, A Hossein-nezhad, Z Maghbooli, K Mirzaei, F Karimi
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  ABSTRACT: The aim of this study was to investigate the association of macrophage migration inhibitory factor (MIF) polymorphism rs1007888 with gestational diabetes mellitus (GDM), and its association with postpartum metabolic syndrome. In a case-control study, 147 GDM and 169 healthy pregnant patients were recruited. Blood sample was taken 2 times from all the participants; one at 24-28 weeks of gestation, second at 6-12 weeks of postpartum. Biochemical measurement and DNA extraction were performed. The PCR_SSP was performed for genotyping. The frequencies of AA, AG, and GG genotypes were 11.24% (19), 76.92% (130), and 11.83% (20) in healthy pregnancies and were 7.48% (11), 70.74% (104), and 21.76% (32) in GDM individuals. The distributions of MIF genotypes were significantly different in GDM and healthy subjects (p=0.04). Moreover, GG genotype had a significant association with pre-pregnancy obesity and family history of diabetes. In postpartum follow-up GG genotype was two-fold more frequent in women with metabolic syndrome (p=0.01, odds ratio=2.30, CI 95%; 1.23-4.30) and relative risk was equal 1.77 (CI 95%; 1.19-2.64). Our findings demonstrate an association between MIF polymorphism rs1007888 and susceptibility to GDM in pregnancy and metabolic syndrome development.
  Hormone and Metabolic Research 04/2011; 43(8):557-61. · 2.19 Impact Factor
• Article: Evidence of a role of ANGPTL6 in resting metabolic rate and its potential application in treatment of obesity.

  K Mirzaei, A Hossein-Nezhad, M Chamari, S Shahbazi
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  ABSTRACT: ANGPTL6 (Angiopoietin-related growth factor 6) is a circulating protein which is suggested to antagonize obesity. The purpose of this study was to evaluate a potential relationship between fasting serum ANGPTL6 and resting metabolic rate (RMR) as well as the body composition in obese and subjects with normal weight. Participants were 62 obese and 41 non-obese subjects who were assessed following an overnight fasting for RMR by means of indirect calorimetry. Body composition was measured using Bodystat devise. Serum ANGPTL6 levels were quantified by ELISA method. Based on ROC analysis best RMR/kg cut-off value for predicting the risk of obesity was 20 kcal/24h /kg. The participants with RMR/kg≥20 kcal/24h/kg were considered as and subjects with RMR/kg<20 kcal/24h/kg were categorized as. In group I, 72.3% of subjects were obese, whereas, 47.4% subjects in group II were suffering from the disease. Participants in group II who showed significantly lower HDL and ANGPTL6 levels. Moreover, we found significantly higher triglyceride and hs-CRP levels in this group. There was significant difference in weight, body mass index, fat mass, visceral fat, RMR/kg, fasting serum glucose, insulin and hs-CRP among those with different levels of the serum ANGPTL6 concentration. We found higher values of RMR/kg in subjects with higher circulating ANGPTL6 concentration. ANGPTL6 affects RMR and significantly improves lipid profile and slightly does so regarding insulin concentrations and sensitivity to it. Further study is warranted as it seems that the results of this study might potentially lead to advent of a pharmacological treatment for obesity.
  Minerva endocrinologica 03/2011; 36(1):13-21. · 0.98 Impact Factor
• Article: Visfatin genotype may modify the insulin resistance and lipid profile in type 2 diabetes patients.

  K Mirzaei, A Hossein-Nezhad, M Javad Hosseinzadeh-Attar, N Jafari, A Najmafshar, N Mohammadzadeh, B Larijani
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  ABSTRACT: We investigated the role of the -4689G/T promoter variant of the visfatin gene on serum visfatin concentration and biochemical markers in T2DM patient. In a cross-sectional study we recruited 93 patients with type 2 diabetes. Laboratory and anthropometric measurements were included FBG, OGTT, HbA1C, lipid Profile, fasting serum visfatin, fasting serum insulin, weight, height, Body Mass Index (BMI) and waist hip ratio (WHR). Genotyping for visfatin gene was performed by using the PCR-RFLP method. Our findings showed significant differences in levels of low density lipoprotein (LDL) cholesterol, total cholesterol, high density lipoprotein (HDL) cholesterol and fasting serum insulin among various types of visfatin genotype (TT, GG, and GT). This study showed a significant correlation between circulating levels of visfatin and weight, BMI, hs-CRP and fasting insulin in TT genotype. But regarding GG genotype only fasting insulin had a significant correlation with circulating visfatin. Visfatin genotypes may account for insulin resistance and levels of lipid profile that may cause by different visfatin expression between genotypes.
  Minerva endocrinologica 12/2009; 34(4):273-9. · 0.98 Impact Factor
• Source

  Available from: tums.ac.ir

  Article: < em> Relationship between Mandibular BMD and Bone Turnover Markers in Osteoporosis Diagnosis

  Sm Eshaghi, A Hossein-Nezhad, Zh Maghbooli, B Larijani
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  ABSTRACT: Background: The purpose of the present study was to determine mandible bone mineral density and evaluate its correlation with central BMD and bone turnover. Methods: Two hundred and seven postmenopausal women were enrolled in this cross-sectional study. After receiving the testimonials, questionnaires were completed and physical exams were done. For all participants central BMD was measured through DXA method. In each women periapical radiography performed in two regions of mandible. The plain x-ray films were scanned using a standard film digitizer and standardized in size and intensity using a calibration step wedge phantom. The phantom was placed upper site in film cover. After the film digitized, the developed Matlab software was used to image processing. Results: Mean age and body mass index of participants were 54.6±6.3 years and 28.57±4.9 kg/m2 respectively. Prevalence of osteoporosis and osteopenia in one of regions in central DXA were 17.4% and 48.2% respectively. There was strong cor-relation between mandible and total femur BMD (P= 0.001, r= 0.80).In osteoporotic patients bone loss in mandible BMD was more than central DXA (P= 0.02). Conclusion: The main advantage of the proposed mandible BMD is to help clinicians make more accurate evaluation of Bone loss. Based on developed the suggested system a routine dental X-ray could be used to screen for bone loss.
  01/2008;
• Article: Relationship between leptin concentration and insulin resistance.

  Z Maghbooli, A Hossein-Nezhad, M Rahmani, A-R Shafaei, B Larijani
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  ABSTRACT: Available evidences suggest that leptin has inhibitory role on insulin secretion. The aim of the work was to examine the association between plasma leptin concentrations and insulin resistance in patients with gestational diabetes mellitus. As a cross-sectional study we recruited 741 pregnant women. The universal screening was performed with an oral glucose challenge test-50 g. The recruits with plasma glucose levels of > or = 7.2 mmol/l were diagnosed as having gestational diabetes mellitus if they had an impaired oral glucose tolerance test-100 g based on Carpenter and Coustan criteria. In all pregnancies plasma insulin and leptin concentrations were measured. Gestational diabetes mellitus developed in 7% (52) of pregnancies. Elevated leptin concentrations were positively associated with insulin levels, BMI, and HOMA index while it was negatively associated with Quicky index. After adjusting for age and BMI before pregnancy, gestational diabetes mellitus had independent direct correlation with leptin concentration. Indeed, leptin level equal to or more than 20 ng/ml could help to predict the developing gestational diabetes mellitus. Measurement of leptin together with the assessment of other risk factors could help identifying women at risk of developing GDM.
  Hormone and Metabolic Research 01/2008; 39(12):903-7. · 2.19 Impact Factor
• Article: Bone mineral density and estimated height loss based on patients' recalls.

  A Moayyeri, S Ahmadi-Abhari, A Hossein-nezhad, B Larijani, A Soltani
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  ABSTRACT: Height loss has been shown to be an indicator of incident vertebral fractures. However, the relationship between height loss and bone mineral density (BMD) in different skeletal regions, as well as the power of human memory in estimation of height loss across the life span, has not yet been established. Given that the variation in BMD between populations is substantially less than the variation in fracture risk, we studied the relationship between height loss based on patient's recalls and BMD in Iranian men and women of all ages. Randomized clustered sampling from all regions of Tehran was performed to recruit the study population. Participants were asked about their maximum recalled previously measured height, if they were confident. In the 457 participants included, the difference between the participants' maximum recalled and current measured height was calculated. L1-L4 lumbar BMD, femoral neck BMD, and young adjusted T-scores were significantly lower in the group of participants with estimated height reduction of greater than 5 cm. In simple linear regression analysis, height loss was a significant predictor of femoral neck T-score (standardized beta coefficient=-0.15; p0.003) and L1-L4 lumbar T-score (beta=-0.08; p0.048). After adjustment for age, gender, and weight, height loss remained a significant predictor for femoral neck T-score (beta=-0.078; p0 .043). In multivariate models for lumbar T-score, height loss was an independent predictor only in participants equal to or younger than 50 years of age (beta=-0.144; p0.033). Higher estimated height loss according to patients' recalls was an indicator of lower BMD in our sample. Especially in the femoral neck region, this factor might be considered as a substitute case-finding tool for low BMD. Considering relatively young nature of our study group and biological differences between populations, our findings need to be validated in future prospective studies.
  Osteoporosis International 02/2006; 17(6):834-40. · 4.58 Impact Factor
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  Available from: Vahid Haghpanah

  Article: Epidemiologic analysis of the Tehran Cancer Institute Data System Registry (TCIDSR).

  B Larijani, M Shirzad, M A Mohagheghi, V Haghpanah, A R Mosavi-Jarrahi, S M Tavangar, A R Vassigh, A Hossein-Nezhad, F Bandarian, R Baradar-Jalili
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  ABSTRACT: To review epidemiological data on thyroid cancer in Iran. The Tehran Cancer Institute Data System Registry (TCIDSR) was used to identify patients with different histological types of thyroid cancer (TC) in Iran. Data were analysed from 438 thyroid cancer cases identified by the TCIDSR in 1998-99. Disease prevalence was calculated with reference to age, time and place. The TCIDSR recorded 438 primary malignancies of the thyroid gland: papillary, follicular, medullary, and anaplastic carcinomas accounted for 67.1%, 10.7%, 5.3% and 4.3% of cases, respectively. The remaining 12.6% were classified as OD (other diagnoses). The prevalence of TC was highest in ethnic Farsis. The age range of patients was 8-85 years. Mean patient age was 44.52+17.03 years (mean + SD) overall, 47.74+18.10 years in female patients and 43.04+16.34 years in male patients. Anaplastic (6.5% vs. 3.3%) and medullary (10.0% vs. 3.0%) cancers were more common in men than women. This study was undertaken to define the epidemiological aspects of thyroid carcinoma in Iran, an area of endemic iodine deficiency until fairly recently. Against expectation for an iodine-deficient area, the frequency distribution of tumours in our study was closer to that seen in iodine-rich areas. Additional research on the risk factors for thyroid cancer--genetic, ethnic, geographic and environmental--is needed to explain the high incidence of PTC overall, and among ethnic Farsis in particular, in Iran.
  Asian Pacific journal of cancer prevention: APJCP 5(1):36-9. · 0.66 Impact Factor