Publications (22)252.56 Total impact
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Article: Characterization of the pufferfish (Fugu) genome as a compact model vertebrate genome.
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ABSTRACT: Cloning and sequencing techniques now allow us to characterize genes directly instead of having to deduce their properties from their effects. This new genetics reaches its apotheosis in the plan to obtain the complete DNA sequence of the human genome, but this is far beyond the capacity of present sequencing methods. Small 'model' genomes, 'such as those of Escherichia coli (4.7 megabases (Mb) and yeast (14 Mb), or even those of Caenorhabditis elegans (100 Mb) and Drosophila (165 Mb), are better scaled to existing technology. The yeast genome will contain genes with functions common to all eukaryotic cells, and those of simple multicellular organisms may throw light on the genetic specification of more complex functions. However, vertebrates differ in their morphology and development, so the ideal model would be a vertebrate genome of minimum size and complexity but with maximum homology to the human genome. Here we report the characterization of the small genome (400 Mb) of the tetraodontoid fish, Fugu rubripes. A random sequencing approach supported by gene probing shows that the haploid genome contains 400 Mb of DNA, of which more that 90% is unique. This genome is 7.5 times smaller than the human genome and because it has a similar gene repertoire it is the best model genome for the discovery of human genes.Nature 12/1993; 366(6452):265-8. · 36.28 Impact Factor -
Article: Comparative analysis of the polycystic kidney disease 1 (PKD1) gene reveals an integral membrane glycoprotein with multiple evolutionary conserved domains
Top co-authors
Top Journals
Institutions
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2010
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Vancouver General Hospital
Vancouver, British Columbia, Canada
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1996–2007
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University of Cambridge
- • Department of Oncology
- • Cambridge Institute for Medical Research
- • Department of Medicine
Cambridge, ENG, United Kingdom -
London Research Institute
London, ENG, United Kingdom
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1997–2000
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Medical Research Council (UK)
London, ENG, United Kingdom -
Cambridge University Hospitals
Cambridge, ENG, United Kingdom
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1994–1995
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MRC National Institute for Medical Research
- Division of Developmental Neurobiology
London, ENG, United Kingdom
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1993
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Addenbrooke's Hospital
Cambridge, ENG, United Kingdom
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