Hae-Ryong Park

Kyungnam University, Changnyeong, Gyeongsangnam-do, South Korea

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Publications (62)93.26 Total impact

  • Jeung-Min Lee · Dahee Koo · Hae-Ryong Park
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    ABSTRACT: Many successful therapeutic drug, including several anticancer agents, have been isolated for natural products. In a recent screen of methanolic extracts from several natural products, we isolated an active compound, from the methanolic extract of persimmon calyx, which promoted cytotoxicity and apoptosis in HT-29 human colon cancer cells. This compound was identified as 28-oxoallobetulin, a prototypical compound, by UV-MS analysis and various NMR spectroscopic methods. The highly cytotoxic effects of 28-oxoallobetulin on HT-29 cells were demonstrated using MTT reduction, LDH release, and colony formation assays. Furthermore, we were able to detect apoptotic bodies in HT-29 cells by Hoechst staining as well as flow cytometric analysis for the presence of sub-G1 DNA peaks, indicative of apoptotic cells. Finally apoptosis in HT-29 cells following 28-oxoallobetulin treatment was also confirmed by the presence of activated caspase-3 and specific proteolytic cleavage of poly (ADP-ribose) polymerase.
    Food science and biotechnology 08/2014; 23(4):1321-1325. DOI:10.1007/s10068-014-0181-6 · 0.66 Impact Factor
  • Eun-Soon Son · Jeong-Un Choi · Kyung-Jin Lee · Hae-Ryong Park
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    ABSTRACT: In this study, the selective cytotoxic activity of methanolic extracts of Saururus chinensis (SCE) was examined on glucose-deprived HT-29 cells. The effects of SCE on 2-deoxy-glucose (2DG) or glucose-free stressed HT-29 cells were evaluated through the MTT reduction assay, morphological observations, and the colony formation assay, in which SCE (5, 10 μg/mL) was found to be highly toxic to HT-29 cells only during glucose-deprived conditions. In addition, the mechanism of selective cytotoxic effects on SCE was assessed by Western blot analysis; SCE suppressed the accumulation of GRP78. Furthermore, apoptotic effects of SCE on glucose-deprived HT-29 cells were identified by Hoechst 33342 staining and flow cytometric analysis. In conclusion, SCE induced apoptosis in HT-29 cells under glucose-deprived conditions by down-regulation of GRP78. Cytotoxic effects on HT-29 cells were only observed during glucose deprivation, suggesting that SCE may be a target for novel therapeutic agents for treating colon cancer under glucose-deprived stress.
    Food science and biotechnology 04/2014; 23(2):609-613. DOI:10.1007/s10068-014-0083-7 · 0.66 Impact Factor
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    ABSTRACT: In our study, each part (flesh, white rind, and green rind) of watermelon was extracted using hydrothermal extraction method at temperatures ranging from 100 to at the intervals of 10, 30, and 60 min. We found that hydrothermal treatment has a significant bearing not only on tyrosinase inhibitory activity but also on neuronal cell protection of watermelon parts. The peak tyrosinase inhibitory activity (about 93%) was observed in both the flesh and green rind extracts at for 60 min. In addition, we observed that hydrothermal extracts of watermelon parts at for 60 min also evidenced significant protection effect for neuronal cell against in a concentrationdependent manner. The results of this study confirm that hydrothermal treatment may be an efficient processing method for the purpose of obtaining potent bioactive substances from watermelon.
    Journal of the Korean Society of Food Science and Nutrition 10/2013; 42(10). DOI:10.3746/jkfn.2013.42.10.1707
  • Youngkyoung Kang · Jeong-Un Choi · Eun-Ah Lee · Hae-Ryong Park
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    ABSTRACT: In the course of screening program for skin whitening compounds, flaniostatin (FST) was isolated from the leaves of Cudrania tricuspidata as a novel inhibitor of tyrosinase. The structure of FST was determined by ESI-MS and NMR spectroscopic analyses as a new isoflavone glycoside. The FST was exhibited a tyrosinase inhibitory effect in a dose-dependent manner. This effect was higher than that of arbutin at the same concentrations. These results indicated that FST isolated from C. tricuspidata may be a positive tool for skin-whitening agent research.
    Food science and biotechnology 10/2013; 22(5):1-4. DOI:10.1007/s10068-013-0236-0 · 0.66 Impact Factor
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    Young-Kyoung Kang · Eun-Ah Lee · Hae-Ryong Park
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    ABSTRACT: In an attempt to identify the neuroprotective effect of Cudrania tricuspidata (CT) leaves against ROS (reactive oxygen species)-induced oxidative stress in neuronal cells, the extracts from CT leaves were investigated using PC12 cells and N18-RE-105 cells. The methanolic and ethanolic extracts from CT were denoted as CTM (Cudrania tricuspidata Leaves methanolic extracts) and CTE (Cudrania tricuspidata Leaves ethanolic extracts), respectively. The neuroprotective effects of the extracts were measured by DCF-DA assay, MTT reduction assay, and LDH release assay. The PC12 cells exposed to -induced oxidative stress and the N18-RE-105 cells exposed to glutamate-induced oxidative stress were treated with various concentrations of CTM and CTE. The results, CTM treatments resulted in the induction of a dose-dependent protective effect in PC12 cells and N18-RE-105 cells. Interestingly, CTE also showed neuroprotective effect in PC12 cells and N18-RE-105 cells. Therefore, these results suggest that CTM and CTE could be a new potential candidate as neuroprotective agents against ROS-induced oxidative stress in neuronal cells.
    12/2012; 28(6). DOI:10.9724/kfcs.2012.28.6.821
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    ABSTRACT: Inhibiting the unfolded protein response (UPR) can be a therapeutic approach, especially for targeting the tumor microenvironment. Here, we show that compound C (also known as dorsomorphin), a small-molecule inhibitor of AMP-activated protein kinase (AMPK) and bone morphogenetic protein (BMP) signaling, inhibit the UPR-induced transcription program depending on the glucose deprivation conditions. We found that compound C prevented UPR marker glucose-regulated protein 78 (GRP78) accumulation and exerted enhanced cytotoxicity during glucose deprivation. Gene expression profiling, together with biochemical analysis, revealed that compound C had a unique mode of action to suppress the transcriptional activation of UPR-targeted genes, as compared with the classic UPR inhibitors versipelostatin and biguanides. Surprisingly, the UPR-inhibiting activity of compound C was not associated with either AMPK or BMP signaling inhibition. We further found that combination treatments of compound C and the classic UPR inhibitors resulted in synergistic cell death with UPR suppression during glucose deprivation. Our findings demonstrate that compound C could be a unique tool for developing a UPR-targeted antitumor therapy.
    PLoS ONE 09/2012; 7(9):e45845. DOI:10.1371/journal.pone.0045845 · 3.23 Impact Factor
  • Journal of the Korean Society of Food Science and Nutrition 09/2011; 40(9):1208-1214. DOI:10.3746/jkfn.2011.40.9.1208
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    ABSTRACT: This study was performed to assess the neuroprotective effects of methanolic extracts from sweet persimmon peel (PPE) against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. The neuroprotective effects of PPE in N18-RE-105 cells were measured using the MTT reduction assay, LDH release assay, and phase-contrast microscopy. The results of the MTT reduction assay showed that treating cells with 500 PPE resulted in cell viability of 66.9%. Additionally, the morphological changes and the results of the LDH release assay showed that glutamate-induced damage to nerve cells was strongly inhibited by PPE. GSH content of N18-RE-105 cells was 3.5 compared to that of the control, whereas pretreatment with 500 PPE increased GSH content by 4.7 . PPE was fractionated with hexane, and that layer had the highest neuroprotective effects in glutamate-stressed N18-RE-105 cells. In conclusion, our data showed that glutamate potentiated the effects of N18-RE-105 cell death by a mechanism involving oxidative stress. Therefore, PPE may be a potential candidate for prevention and therapy of neurodegenerative diseases.
    08/2011; 27(4). DOI:10.9724/kfcs.2011.27.4.067
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    ABSTRACT: 4,8-Dihydroxy-5-methoxy-2-naphthaldehyde (Compound I) was isolated from blackened heartwood of Diospyros kaki and was methylated with diazomethane. Antioxidant and cytotoxic activities of Compound I and two methylated derivatives [4-hydroxy-5,8-dimethoxy-2-(2-oxopropyl)-naphthalene (D-1) and 2-glycidyl-4-hydroxy-5,8-dimethoxy naphthalene (D-2)] were evaluated. Compound I showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power than D-1 and D-2. However, D-1 and D-2 exhibited slightly stronger 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)+ (ABTS+) radical scavenging activity than Compound I. Compound I also exhibited stronger cytotoxic activity than D-1 and D-2 against the growth of HT-29 colon cancer cells. The results supported the hypothesis that methylation of naphthalene derivatives slightly increased ABTS+ radical scavenging activity, but significantly decreased DPPH radical scavenging activity, reducing power, and cytotoxic activity. Key wordsNaphthalene derivatives–Persimmon wood–Antioxidant activity–Cytotoxic activity
    Journal of Wood Science 04/2011; 57(2):161-165. DOI:10.1007/s10086-010-1147-9 · 0.83 Impact Factor
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    ABSTRACT: This study was performed to investigate the physiological activities of stings of Gleditsia sinensis extracts. Antioxidant activity was evaluated by measuring total phenolic contents (TPC), comet assay, and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA). Anti-diabetic activity was measured by inhibition activities on -glucosidase. Stings of Gleditsia sinensis extracts were prepared by extracting them with methanol and ethanol. The methanolic extracts showed the highest phenol content (1.12 g/100 g gallic acid equivalents). The -glucosidase inhibitory activity of methanol extracts were 17.9% higher, and that of ethanol extracts were 10.3% higher at a concentration of 1 mg/ml. These results indicate that stings of Gleditsia sinensis might be potential candidates as antioxidant and anti-diabetic agents.
    01/2011; 21(1). DOI:10.5352/JLS.2011.21.1.62
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    ABSTRACT: Postmortem examinations of tissues of humans and rodents with a host of neurodegenerative conditions, including Alzheimer's and Parkinson's diseases, have identified oxidative damage in proteins, lipids, and DNA. The aim of this study was to better understand the cellular mechanisms of neuronal cell degeneration induced via oxidative stress and the protective roles of bioactive substance. In order to achieve this aim, we established a screening program to discover therapeutic agents that exhibit preferential neuroprotective activity in H(2)O(2)-treated PC12 cells. During the course of our screening program, we isolated an active compound, SG-168, from Dendrobium nobile Lindley and identified it as a neuroprotective agent. SG-168 was identified as a compound with an acetal skeleton, a prototypical compound, by electrospray ionization-mass spectrometry analysis and various nuclear magnetic resonance spectroscopic methods. The protective effect of SG-168 in PC12 cells with H(2)O(2)-induced oxidative damage was investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay. As expected, incubation with H(2)O(2) for 2 hours resulted in cell viability of 31.8% compared to the control, while pretreatment of SG-168 increased cell viability by 15-50% compared to the H(2)O(2)-stressed control cells. These results showed that SG-168 inhibits H(2)O(2)-induced apoptotic cell death. Interestingly, flow cytometric analysis showed that H(2)O(2)-treated PC12 cells incubated with SG-168 exhibited greatly suppressed apoptosis. In summation, the results of this study suggest that SG-168 has potential as a new antioxidant agent against neuronal diseases.
    Journal of medicinal food 01/2011; 14(1-2):120-7. DOI:10.1089/jmf.2010.1027 · 1.70 Impact Factor
  • Hae-Ryong Park · Kazuo Furihata · Yoichi Hayakawa · Kazuo Shin-ya
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    ABSTRACT: ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
    ChemInform 12/2010; 33(49). DOI:10.1002/chin.200249175
  • Jeung-Min Lee · Jae-Hee Park · Hae-Ryong Park · Eun-Ju Park
    Journal of the Korean Society of Food Science and Nutrition 09/2010; 39(9):1243-1248. DOI:10.3746/jkfn.2010.39.9.1243
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    ABSTRACT: Cancer cells in poorly vascularized solid tumors are constantly or intermittently exposed to stressful microenvironments, including glucose deprivation, hypoxia, and other forms of nutrient starvation. These tumor-specific conditions, especially glucose deprivation, activate a signaling pathway called the unfolded protein response (UPR), which enhances cell survival by induction of the stress proteins. We have established a screening method to discover anticancer agents that could preferentially inhibit tumor cell viability under glucose-deprived conditions. Here we identify arctigenin (ARC-G) as an active compound that shows selective cytotoxicity and inhibits the UPR during glucose deprivation. Indeed, ARC-G blocked expression of UPR target genes such as phosphorylated-PERK, ATF4, CHOP, and GRP78, which was accompanied by enhanced phosphorylation of eIF2 alpha during glucose deprivation. The UPR inhibition led to apoptosis involving a mitochondrial pathway by activation of caspase-9 and -3. Furthermore, ARC-G suppressed tumor growth of colon cancer HT-29 xenografts. Our results demonstrate that ARC-G can be served as a novel type of antitumor agent targeting the UPR in glucose-deprived solid tumors.
    Journal of Cellular Physiology 07/2010; 224(1):33-40. DOI:10.1002/jcp.22085 · 3.87 Impact Factor
  • Journal of the Korean Society of Food Science and Nutrition 04/2010; 39(4):500-505. DOI:10.3746/jkfn.2010.39.4.500
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    Sang-Bong Lee · Hae-Ryong Park
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    ABSTRACT: Natural products have recently become the focus of increased research interest due to their potential pharmacological activities. Therefore, we established a program to screen natural products for cytotoxic activity using the MTT reduction assay system to test HT-29 human colon cancer cells. During the course of screening, we found that the acetone extracts of guava (Psidium guajava L.) branch (GBA) had cytotoxic effects on HT-29 cells. The GBA showed highly cytotoxic effects via the MTT reduction assay, LDH release assay, and colony formation assay. In particular, the GBA of the 250 µ µ µ µg/ml showed 35.5% inhibition against growth of HT-29 cells. As expected, GBA induced characteristic apoptotic effects in HT-29 cells, including chromatin condensation and sharking that occurred 24 h after the cells had been treated at a concentration level of 250 µ µ µ µg/ml. To examine the functions on apoptosis, we used a flow cytometric analysis. The apoptotic cells were distributed according to the cell cycle phase shown by sub-G1 DNA content.
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    Hyun-Jung Kim · Jum-Soon Kang · Hae-Ryong Park · Yong-Il Hwang
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    ABSTRACT: The neuroprotective effects of extracts from various parts of peanut sprouts on glutamate-induced neurotoxicity in N18-RE-105 cells were investigated. This study was performed to evaluate the neuroprotective activity of methanolic extracts from the whole (WME), heads (HME), and stems (SME) of peanut sprouts. The neuroprotective effects of these extracts were measured by MTT reduction assay, LDH release assay, phase-contrast microscopy, and flow cytometric analysis on the N18-RE-105 cells. Among these extracts, the HME showed the greatest neuroprotective effects, and was further fractionated with hexane, diethyl ether, ethyl acetate, and water, according to degree of polarity. Out of the fractionated extracts, the diethyl ether layer showed the highest activity on glutamate-induced cytotoxicity in N18-RE-105 cells. The sub-G1 DNA contents of the glutamate-induced severely apoptotic N18-RE-105s were measured by flow cytometric analysis to confirm the HME's anti-apoptotic activity. Interestingly, after incubation with 100 mg/ml of the HME, the proportion of sub-G1 cells of the glutamate-stressed N18-RE-105s had been greatly reduced, from 58.5% to 9.1%. These results imply that HME may have strong potential as a chemotherapeutic agent against neuronal diseases.
    02/2010; 20(2). DOI:10.5352/JLS.2010.20.2.253
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    Hae-Ryong Park · Eunju Park · A-Ram Rim · Kyung-Im Jeon · Ji-Hwan Hwang
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    ABSTRACT: Antioxidants play an important role in inhibiting and scavenging radicals, thus providing protection to humans against infectious and degenerative diseases. Literature shows that the antioxidant activity is high in medicinal plants. Realizing the fact that, this study was carried out to determine the antioxidant activity of water extract of Acanthopanax senticosus. Water extract (0.5 g/50 ml) of A. senticosus (ASE) were prepared and total phenol contents (TPC) and radical scavenging activity (RSA) of the extracts was determined for antioxidant activity. The TPC and RSA of ASE were 366.67 μM and 67.67%, respectively. In addition, the effect of ASE on DNA damage induced by H 2O2 in human lymphocytes was also evaluated by Comet assay. The ASE showed strong inhibitory effect as its concentration increased from 0.125 to 1% by 65 to 81% against DNA damage induced by 200 μM of H 2O2. These results suggest that water extract of commercial dried A. senticosus for tea showed significant antioxidant activity and protective effect against oxidative DNA damage.
    AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2010; 5(23). · 0.57 Impact Factor
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    ABSTRACT: Oyster mushroom is a popular edible mushroom which has various colorful fruit bodies. The objective of this study was to determine the antioxidant and the anticancer activities of oyster mushrooms (OM) with different colors such as dark-grey strain (Pleurotus ostreatus), yellow strain (Pleurotus cornucopiae), and pink strain (Pleurotus salmoneostramineus). The methanolic extracts from OMs were prepared for this study. Among these OMs, the extract from the yellow strain showed the highest radical scavenging activity, reducing power, ferrous chelating ability, and total phenolic contents. Radical scavenging activity of yellow strain was about 3 times higher than that of dark-grey strain. On the other hand, the extracts of dark-grey and pink strains showed higher suppressive effect against growth of human colon cancer cell line HT-29 with survival rates of 39.9 and 40.7%, respectively, than that of yellow strain. These results showed that the antioxidant and the anticancer activities of OMs varied by the colors of fruit bodies.
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    Hyun-Jung Kim · Jeung-Min Lee · Seong-Hee Moon · Hae-Ryong Park
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    ABSTRACT: The oxidative stress induced by reactive oxygen species (ROS) may play an important role in the pathogenesis of neurodegenerative diseases. In this study, we investigated the neuroprotective effects of methanolic extracts of Prunella Spica (PSE) against -induced oxidative stress in PC12 cells. The cells exposed to -induced oxidative stress were treated with various concentrations of PSE; this treatment resulted in the induction of a dose-dependent protective effect, which was evidenced by the results of MTT reduction assay, lactate dehydrogenase (LDH) release assay, morphological assay, and colony-formation assay. Interestingly, we also observed reduction of apoptotic bodies in the Hoechst staining and flow cytometric analysis. These data show that apoptosis was significantly suppressed in the PC12 cells that were exposed to -induced oxidative stress and treated with PSE. These results suggest that Prunella Spica could be a new potential protective agent against -induced oxidative stress.
    01/2010; 20(7). DOI:10.5352/JLS.2010.20.7.1121

Publication Stats

784 Citations
93.26 Total Impact Points

Institutions

  • 2005–2014
    • Kyungnam University
      Changnyeong, Gyeongsangnam-do, South Korea
  • 2007–2010
    • The University of Tokyo
      • Institute of Molecular and Cellular Biosciences
      Edo, Tōkyō, Japan
  • 2003–2007
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • • Functional Metabolomics Research Center
      • • Biological Resource Center
      Anzan, Gyeonggi-do, South Korea