Yuan-Shiun Chang

China Medical University Hospital, 臺中市, Taiwan, Taiwan

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Publications (56)124.35 Total impact

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    ABSTRACT: Lobelia chinensis Lour (LcL) is a popular herb that has been widely used as folk medicine in China for the treatment of fever, lung cancer, and inflammation for hundreds of years. Recently, several studies have shown that the anti-inflammatory properties were correlated with the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from the NF-kB pathway. The aim of this study was to evaluate the anti-oxidative and anti-inflammatory activities of L. chinensis. Both suppressive activities on LPS-induced nitric oxide production in RAW264.7 macrophages in vitro and the acute rat lung injury model in vivo were studied. The results showed that the menthanol extract of LcL and its fractions within the range of 62.5-250 μg/mL did not induce cytotoxicity (p < 0.001). The ethyl acetate fraction of LcL showed better NO inhibition activity than other fractions. On the other hand, the Lc-EA (62.5, 125, 250 mg/kg) pretreated rats showed a decrease in the pro-inflammatory cytokines (TNF-α, IL-β, IL-6) and inhibited iNOS, COX-2 expression through the NF-kB pathway. These results suggested that L. chinensis exhibited an anti-inflammatory effect through the NF-kB pathways.
    The American Journal of Chinese Medicine 03/2015; DOI:10.1142/S0192415X15500184 · 2.63 Impact Factor
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    ABSTRACT: Apigenin-7-glycoside (AP7Glu) with multiple biological activities is a flavonoid that is currently prescribed to treat inflammatory diseases such as upper respiratory infections. Recently, several studies have shown that its anti-inflammatory activities have been strongly linked to the inhibition of secretion of pro-inflammatory proteins, such as inducible nitric oxide synthase (iNOs) and cyclooxygenase-2 (COX-2) induced through phosphorylation nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPK) pathways. Additionally, inflammation, which can decrease the activities of antioxidative enzymes (AOEs) is also observed in these studies. At the same time, flavonoids are reported to promote the activities of heme oxygenase-1 (HO-1) decreased by LPS. The purpose of this study was to assess these theories in a series of experiments on the suppressive effects of AP7Glu based on LPS-induced nitric oxide production in RAW264.7 macrophages in vitro and acute lung injury in mice in vivo. After six hours of lipopolysaccharide (LPS) stimulation, pulmonary pathological, myeloperoxidase (MPO) activity, total polymorphonuclear leukocytes (PMN) cells, cytokines in bronchoalveolar lavage fluid (BALF) and AOEs, are all affected and changed. Meanwhile, our data revealed that AP7Glu not only did significantly inhibit the LPS-enhanced inflammatory activity in lung, but also exhibited anti-inflammatory effect through the MAPK and inhibitor NF-κB (IκB) pathways.
    International Journal of Molecular Sciences 01/2015; 16(1):1736-1754. DOI:10.3390/ijms16011736 · 2.34 Impact Factor
  • Yang Zhao, Yuan-Shiun Chang, Pei Chen
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    ABSTRACT: A flow-injection mass spectrometric metabolic fingerprinting method in combination with chemometrics was used to differentiate Aurantii Fructus Immaturus from its counterfeit Poniciri Trifoliatae Fructus Immaturus. Flow-injection mass spectrometric (FIMS) fingerprints of 9 Aurantii Fructus Immaturus samples and 12 Poniciri Trifoliatae Fructus Immaturus samples were acquired and analyzed using principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA). The authentic herbs were differentiated from their counterfeits easily. Eight characteristic components which were responsible for the differences between the samples were tentatively identified. Furthermore, three out of the eight components, naringin, hesperidin, and neohesperidin, were quantified. The results are useful to help identify the authenticity of Aurantii Fructus Immaturus. Published by Elsevier B.V.
    Journal of Pharmaceutical and Biomedical Analysis 12/2014; 107C:251-257. DOI:10.1016/j.jpba.2014.12.035 · 2.83 Impact Factor
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    ABSTRACT: Catalase is an important enzyme performs decomposition of two molecular of hydrogen peroxide to water molecules and oxygen in aerobic organism. Deficiency or inactive catalase are implicated cell damage and lead to inflammation, aging and cancer. In order to develop novel nature product that prevent inactive catalase generation, the world largest traditional Chinese medicine (TCM) database (http://tcm.cmu.edu.tw/) were employed to this study, which combined with high-throughput virtual screening and molecular dynamics (MD) simulation to investigate potent nature compounds for keeping catalase active. We found the two nature product, Hesperidin and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), the found ligands perform high binding affinity with catalase. The results of MD simulation show that THSG is the most stable in trajectory analysis over all simulation times. Besides, THSG can affect the catalase structure more compact during the process of MD simulation. In addition, the radical scavenging assay showing that THSG has more potential antioxidant activity than Hesperidin. Therefore, we regard the nature TCM compound, THSG, could be used to develop potential drugs that might have similar effect to keep catalase active and prevent the inactive form generation by hydrogen peroxide.
    RSC Advances 12/2014; 5(9). DOI:10.1039/C4RA14734K · 3.71 Impact Factor
  • Yi-Kung Lin, Yu-Ling Ho, Yang Zhao, Yuan-Shiun Chang
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    ABSTRACT: The present paper describes the development of a high performance liquid chromatography-ultraviolet (HPLC-UV) detection method for quantitative determination of peimine and peiminine in Fritillariae Thunbergii Bulbus (FTB). Separation was achieved using a conventional XBridgeTM Shield RP 18 column (250 mm × 4.6 mm, internal diameter 3.5 μm) with photodiode array detection at 190–400 nm for UV spectra and 220 nm for quantification. The mobile phase consisted of (A) 0.03% diethylamine aqueous solution and (B) acetonitrile eluted by an isocratic procedure at 45:55 (A:B) over 25 minutes. The method was validated for linearity, limits of detection (LOD) and quantification (LOQ), inter- and intra-day precisions, repeatability, stability, and recovery. All the validation results were satisfactory. The developed method was then applied to assay the contents of the two chemical markers in all the FTB samples collected. Based on the contents of the two analytes, hierarchical clustering analysis (HCA) was performed to reveal the similarities and differences of the samples.
    Journal of Food and Drug Analysis 11/2014; 23(1). DOI:10.1016/j.jfda.2014.06.004 · 0.40 Impact Factor
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    ABSTRACT: This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities.
    Molecules 11/2014; 19(11):18332-50. DOI:10.3390/molecules191118332 · 2.10 Impact Factor
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    ABSTRACT: Ethnopharmacological relevance: Traditional Chinese medicine (TCM) has been commonly used in Asia country. The aim of this study is to analyze the utilization of TCM among hypertensive patients in Taiwan. Materials and methods: The use of TCM for primary hypertensive patients was evaluated using a randomly sampled cohort of 1,000,0s recruited from the National Health Insurance Research Database in 5-year period from 2006 to 2010. Results: Overall, 49.7% (n=42,586) of primary hypertension utilized TCM and 12.1% (n=5132) of them used TCM for the treatment of hypertension. Among the top 10 most frequently prescribed herbal formulae, Tian-Ma-Gou-Teng-Yin, Gout-Teng-San, Liu-Wei-Di-Huang-Wang and its derivatives were found to be the most common herbal formulae prescribed by TCM doctors for the treatment of hypertension in Taiwan. Conclusion: This study showed the utilization pattern of Chinese herbal product in patients with hypertension. Further researches and clinical trials are needed to evaluate the efficacy of these Chinese formulae or its ingredients in treating hypertension.
    Journal of Ethnopharmacology 08/2014; 155(3). DOI:10.1016/j.jep.2014.07.047 · 2.94 Impact Factor
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    ABSTRACT: Elaeagnus oldhamii Maxim. is a commonly used traditional herbal medicine. In Taiwan the leaves of E. oldhamii Maxim. are mainly used for treating lung disorders. Twenty five compounds were isolated from the leaves of E. oldhamii Maxim. in the present study. These included oleanolic acid (1), 3-O-(Z)-coumaroyl oleanolic acid (2), 3-O-(E)-coumaroyl oleanolic acid (3), 3-O-caffeoyl oleanolic acid (4), ursolic acid (5), 3-O-(Z)-coumaroyl ursolic acid (6), 3-O-(E)-coumaroyl ursolic acid (7), 3-O-caffeoyl ursolic acid (8), 3β, 13β-dihydroxyolean-11-en-28-oic acid (9), 3β, 13β-dihydroxyurs-11-en-28-oic acid (10), uvaol (11), betulin (12), lupeol (13), kaempferol (14), aromadendrin (15), epigallocatechin (16), cis-tiliroside (17), trans-tiliroside (18), isoamericanol B (19), trans-p-coumaric acid (20), protocatechuic acid (21), salicylic acid (22), trans-ferulic acid (23), syringic acid (24) and 3-O-methylgallic acid (25). Of the 25 isolated compounds, 21 compounds were identified for the first time in E. oldhamii Maxim. These included compounds 1, 4, 5 and 8-25. These 25 compounds were evaluated for their inhibitory activity against the growth of non-small cell lung cancer A549 cells by the MTT assay, and the corresponding structure-activity relationships were discussed. Among these 25 compounds, compound 6 displayed the best activity against the A549 cell line in vitro (CC50 = 8.56 ± 0.57 μg/mL, at 48 h of MTT asssay). Furthermore, compound 2, 4, 8 and 18 exhibited in vitro cytotoxicity against the A549 cell line with the CC50 values of less than 20 μg/mL at 48 h of MTT asssay. These five compounds 2, 4, 6, 8 and 18 exhibited better cytotoxic activity compared with cisplatin (positive control, CC50 value of 14.87 ± 1.94 μg/mL, at 48 h of MTT asssay). The result suggested that the five compounds might be responsible for its clinical anti-lung cancer effect.
    Molecules 07/2014; 19(7):9515-9534. DOI:10.3390/molecules19079515 · 2.10 Impact Factor
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    ABSTRACT: Activity-directed fractionation and purification processes were employed to identify the anti-inflammatory active compounds using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) in vitro. Air-dried roots of Ficus formosana were extracted with methanol and separated into n-hexane, chloroform, ethyl acetate, n-butanol, and water layers. Among them, the chloroform layer showed strong activity and was subjected to separation and purification by using various chromatographic techniques. Five compounds showing potent activity were identified by comparing spectral data to be β-sitosterol, stigmasterol, psoralen, kaempferol, carpachromene, and syringic aldehyde. When macrophages were treated with psoralen and kaempferol together with LPS, a concentration-dependent inhibition of nitric oxide (NO) and tumor necrosis factor (TNF-α) productions were detected. Western blotting revealed that kaempferol, psoralen, and carpachromene blocked protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated macrophages. The results confirmed that the traditional use of F. formosana could be a potential anti-inflammatory agent.
    Journal of Agricultural and Food Chemistry 11/2013; 61(46). DOI:10.1021/jf4033766 · 3.11 Impact Factor
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    ABSTRACT: One lignanoid compound, isoamericanol B (1), along with four triterpenoid compounds-cis-3-O-p-hydroxycinnamoyloleanolic acid (2), trans-3-O-p-hydroxy cinnamoyloleanolic acid (3), cis-3-O-p-hydroxycinnamoylursolic acid (4), trans-3-O-p-hydroxycinnamoylursolic acid (5) have been isolated for the first time from the leaves of Elaeagnus oldhamii Maxim. Compounds 1-4 significantly inhibited the expression of NO (nitric oxide) produced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The IC50 value for inhibition of nitrite production of compound 1 was about 10.3 ± 0.4 μg/mL. In the cell viability test, however, among compounds 1-4 compound 1 did not significantly change cell viability. Therefore, in this study compound 1 possessed anti-inflammatory effects. The result suggests compound 1 as a potential lead compound for the treatment of inflammatory diseases.
    Molecules 11/2013; 18(11):13218-27. DOI:10.3390/molecules181113218 · 2.10 Impact Factor
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    ABSTRACT: Enterovirus 71 (EV71) and coxsackievirus A16 (CoxA16) are main pathogens of hand-foot-and-mouth disease, occasionally causing aseptic meningitis and encephalitis in tropical and subtropical regions. Kalanchoe gracilis, Da-Huan-Hun, is a Chinese folk medicine for treating pain and inflammation, exhibiting antioxidant and anti-inflammatory activities. Our prior report (2012) cited K. gracilis leaf extract as moderately active against EV71 and CoxA16. This study further rates antienteroviral potential of K. gracilis stem (KGS) extract to identify potent antiviral fractions and components. The extract moderately inhibits viral cytopathicity and virus yield, as well as in vitro replication of EV71 (IC50 = 75.18 μ g/mL) and CoxA16 (IC50 = 81.41 μ g/mL). Ethyl acetate (EA) fraction of KGS extract showed greater antiviral activity than that of n-butanol or aqueous fraction: IC50 values of 4.21 μ g/mL against EV71 and 9.08 μ g/mL against CoxA16. HPLC analysis, UV-Vis absorption spectroscopy, and plaque reduction assay indicate that eupafolin is a vital component of EA fraction showing potent activity against EV71 (IC50 = 1.39 μ M) and CoxA16 (IC50 = 5.24 μ M). Eupafolin specifically lessened virus-induced upregulation of IL-6 and RANTES by inhibiting virus-induced ERK1/2, AP-1, and STAT3 signals. Anti-enteroviral potency of KGS EA fraction and eupafolin shows the clinical potential against EV71 and CoxA16 infection.
    Evidence-based Complementary and Alternative Medicine 09/2013; 2013:591354. DOI:10.1155/2013/591354 · 2.18 Impact Factor
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    Yang Zhao, Chun-Pin Kao, Yuan-Shiun Chang, Yu-Ling Ho
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    ABSTRACT: Polygoni Multiflori Caulis, the dried caulis of Polygonum multiflorum Thunb., is one of the commonly used traditional Chinese medicines having antioxidant, anti-obesity, anti-inflammatory and antibacterial effects. Polygoni Multiflori Caulis used clinically or circulated on market have great differences in their diameters. However, to the best of our knowledge, no study has been reported on the qualities of Polygoni Multiflori Caulis with different diameters. Systematic HPLC/UV/MS chromatographic fingerprinting and quantitative analytical methods combined with principal component analysis were developed and applied to analyze different Polygoni Multiflori Caulis samples. The contents of 2,3,5,4[prime]-tetrahydroxystilbene-2-O-beta-D-glucoside, the chemical marker for quality control on Polygoni Multiflori Caulis specified in Chinese Pharmacopoeia (2010 edition), were found to have surprising relevance with the samples' diameters for the first time. The finding provides a scientific basis for collecting Polygoni Multiflori Caulis in the best time. Moreover, the diameter can be used as the criterion for quality control on Polygoni Multiflori Caulis as a preliminary step in the future. In addition, scores plot obtained from principal component analysis shows the obvious differences between unqualified Polygoni Multiflori Caulis samples and qualified ones visually, which can be used to single out the unqualified ones with qualified ones efficiently and immediately.
    Chemistry Central Journal 06/2013; 7(1):106. DOI:10.1186/1752-153X-7-106 · 1.66 Impact Factor
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    ABSTRACT: This study rated antiviral activity of Scutellaria baicalensis Georgi (S. baicalensis) extracts against influenza A virus subtypes, for example, pandemic 2009 H1N1, seasonal H1N1 and H3N2. Ethyl acetate (EtOAc) and chloroform extracts inhibited in vitro neuraminidase (NA) enzymatic activity and viral replication more than methanol (MeOH) extract. EtOAc extract demonstrated NA inhibition IC50 values ranging from 73.16 to 487.40 μg/mL and plaque reduction IC50 values ranging from 23.7 to 27.4 μg/mL. Chloroform extract showed antiviral activities with plaque reduction IC50 values ranging from 14.16 to 41.49 μg/mL Time-of-addition assay indicated that EtOAc and chloroform extracts also significantly inhibited virus yields after infection. HPLC analysis demonstrated that baicalin was dominant in the MeOH extract; baicalein and chrysin were rich in the EtOAc and chloroform extracts. Molecular simulation revealed baicalein hydrogen bonding with Glu277 as well as hydrophobic and Van der Waals interactions with Ile222, Arg224, Ser246, and Tyr347 in NA1 active sites of NA1. Baicalein inhibited in vitro replication of influenza A viruses pandemic 2009 H1N1 (IC50 = 0.018 μM) and seasonal 2007 H1N1 using plaque reduction assays. A combination of low-dose baicalein with other anti-influenza agents could be applicable for development of alternative remedies treating influenza A virus infection.
    Evidence-based Complementary and Alternative Medicine 01/2013; 2013:750803. DOI:10.1155/2013/750803 · 2.18 Impact Factor
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    ABSTRACT: Through bioassay-guided fractionation, thirteen compounds (1-13) were isolated from the dry root of Semiaquilegia adoxoides, known as Tiankuizi in traditional Chinese medicine (TCM). Among these, four benzoic acid derivatives (1, 2, 4, 5), one 4,6-dimethoxy-5-methyl-2H-pyran-2-one (10) and one 1,2,3-propanetriol (13) were found for the first time in S. adoxoides. This is the first record of compound 10 from a natural source. 4-Hydroxybenzoic acid (1) and 3,4-dihyroxybenzoic acid (2) showed selective inhibition against elastase release and superoxide anion generation, with IC50 values of 3.20 and 6.21 microg/mL, respectively. Compound 1 had 7-fold better activity than the positive control against elastase release induced by human neutrophils. Overall, our studies demonstrated Tiankuizi (S. adoxoides) as a potential TCM and isolates 1 and 2 as promising lead compounds for neutrophilic inflammatory diseases.
    Natural product communications 12/2012; 7(12):1623-6. · 0.92 Impact Factor
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    ABSTRACT: The present study evaluated the potential genotoxicity of the ethanol extracts from the rhizome of Zingiber zerumbet (L.) Smith (EEZZR) using a standard battery of tests. Chemical analysis with liquid chromatography-tandem mass spectrometry revealed that EEZZR contained Zerumbone (200.3 ± 0.37 μg/g) and 6-gingerol (102.5 ± 0.28 μg/g). There were no increases in the number of revertant colonies with EEZZR at concentrations of 150-5000 μg per plate, regardless of the metabolic activation system (S-9 mix) used in the histidine-dependent auxotrophic mutants of Salmonella typhimurium (strains TA97, TA98, TA100, TA102, and TA1535) compared to the vehicle control. Furthermore, EEZZR at doses of 150-5000 μg mL(-1) did not increase the number of structural aberrations in Chinese hamster lung cells in the presence or absence of S-9 mix. An oral administration of EEZZR to ICR mice, with doses of up to 2000 mg/kg, caused no significant increases in the number of micronucleated polychromatic erythrocytes (MNPCEs) and mean ratio of polychromatic erythrocytes to total erythrocytes. Lastly, RZZEE did not increase the incidence of MNPCEs in bone marrow. Based on these findings, it may be concluded that the use of EEZZR in traditional medicine poses no risk of genotoxicity.
    Evidence-based Complementary and Alternative Medicine 07/2012; 2012:406296. DOI:10.1155/2012/406296 · 2.18 Impact Factor
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    ABSTRACT: Pandemic infection or reemergence of Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) occurs in tropical and subtropical regions, being associated with hand-foot-and-mouth disease, herpangina, aseptic meningitis, brain stem encephalitis, pulmonary edema, and paralysis. However, effective therapeutic drugs against EV71 and CVA16 are rare. Kalanchoe gracilis (L.) DC is used for the treatment of injuries, pain, and inflammation. This study investigated antiviral effects of K. gracilis leaf extract on EV71 and CVA16 replications. HPLC analysis with a C-18 reverse phase column showed fingerprint profiles of K. gracilis leaf extract had 15 chromatographic peaks. UV/vis absorption spectra revealed peaks 5, 12, and 15 as ferulic acid, quercetin, and kaempferol, respectively. K. gracilis leaf extract showed little cytotoxicity, but exhibited concentration-dependent antiviral activities including cytopathic effect, plaque, and virus yield reductions. K. gracilis leaf extract was shown to be more potent in antiviral activity than ferulic acid, quercetin, and kaempferol, significantly inhibiting in vitro replication of EV71 (IC(50) = 35.88 μg/mL) and CVA16 (IC(50) = 42.91 μg/mL). Moreover, K. gracilis leaf extract is a safe antienteroviral agent with the inactivation of viral 2A protease and reduction of IL-6 and RANTES expressions.
    Evidence-based Complementary and Alternative Medicine 05/2012; 2012:503165. DOI:10.1155/2012/503165 · 2.18 Impact Factor
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    ABSTRACT: This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract of Ficus pumila (FP(MeOH)). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showed FP(MeOH) decreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses. FP(MeOH) significantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically, FP(MeOH) abated the level of tissue destruction and swelling of the edema paws. This study indicated anti-inflammatory mechanism of FP(MeOH) may be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally, FP(MeOH) also decreased the level of inflammatory mediators such as IL-1β, TNF-α, and COX-2. HPLC fingerprint was established and the contents of three active ingredients, rutin, luteolin, and apigenin, were quantitatively determined. This study provided evidence for the classical treatment of Ficus pumila in inflammatory diseases.
    Evidence-based Complementary and Alternative Medicine 05/2012; 2012:340141. DOI:10.1155/2012/340141 · 2.18 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the antihyperlipidaemic effects of the ethanol extract of Zingiber zerumbet (L) Smith (EEZZ). After being fed a high-fat diet (HFD) for two weeks, rats were dosed orally with EEZZ (100, 200 or 300 mg/kg) or fenofibrate (100 mg/kg) once daily for eight weeks. EEZZ (300 mg/kg/day) produced effects similar to fenofibrate in reducing body weight gain, visceral fat-pad weights and plasma lipid levels. EEZZ caused reductions in hepatic triglyceride and cholesterol content, and lowered hepatic lipid droplet accumulation and the size of epididymal adipocytes. HFD-induced reductions in the hepatic proteins of peroxisome proliferator-activated receptor (PPAR) α, acyl-CoA oxidase (ACO) and cytochrome P450 isoform 4A1 (CYP4A1) were reversed by EEZZ. These results suggest that EEZZ reduced the accumulation of visceral fat and improved hyperlipidaemia in HFD-fed rats by increasing fatty acid oxidation, an effect which is likely to be mediated via up-regulation of hepatic PPARα.
    Food Chemistry 05/2012; 132(1). DOI:10.1016/j.foodchem.2011.11.022 · 3.26 Impact Factor
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    ABSTRACT: Korean red ginseng and ginsenosides have been claimed to possess wide spectrum of medicinal effects, of which anticancer effect is one. The present study was undertaken to investigate the antimetastatic effect of Korean red ginseng on human hepatoma as well as possible mechanisms. The inhibitory effect of the water extract of Korean red ginseng (WKRG) on the invasion and motility of SK-Hep1 cells was evaluated by the Boyden chamber assay in vitro. Without causing cytotoxicity, WKRG exerted a dose-dependent inhibitory effect on the invasion and motility, but not adhesion, of highly metastatic SK-Hep1 cells. Zymography analyses revealed significant downregulating effects on MMP-2, MMP-9, and uPA activities in SK-Hep1 cells. Western blot analyses also showed that WKRG treatment caused dose-dependent decreases in MMP-2 and MMP-9 protein expressions. Moreover, WKRG increased the levels of TIMP-1, TIMP-2, and PAI-1. The present study not only demonstrated that invasion and motility of cancer cells were inhibited by WKRG, but also indicated that such effects were likely associated with the decrease in MMP-2/-9 and uPA expressions of SK-Hep1 cells.
    Evidence-based Complementary and Alternative Medicine 04/2012; 2012:965846. DOI:10.1155/2012/965846 · 2.18 Impact Factor
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    ABSTRACT: The objective of this study was to evaluate the acute and subacute toxicity (28 days) of the ethanol extract of Z. zerumbet rhizomes (EEZZ) via the oral route in Wistar rats of both sexes. In the acute toxicity study, Wistar rats were administered a single dose of 15 g kg(-1) of body weight by gavage, and were monitored for 14 days. EEZZ did not produce any toxic signs or deaths; the 50% lethal dose must be higher than 15 g kg(-1). In the subchronic toxicity study, EEZZ was administered by gavage at doses of 1000, 2000 and 3000 mg/kg daily for 4 weeks to Wistar rats. The subacute treatment with EEZZ did not alter either the body weight gain or the food and water consumption. The hematological and biochemical analysis did not show significant differences in any of the parameters examined in female or male groups. Necropsy and histopathological examination, did not reveal any remarkable and treatment related changes. A no-observed adverse-effect level for EEZZ is 3000 mg kg(-1) for rats under the conditions of this study. Hence, consumption of EEZZ for various medicinal purposes is safe.
    Evidence-based Complementary and Alternative Medicine 03/2012; 2012:608284. DOI:10.1155/2012/608284 · 2.18 Impact Factor

Publication Stats

350 Citations
124.35 Total Impact Points

Institutions

  • 2007–2014
    • China Medical University Hospital
      • Department of Radiology
      臺中市, Taiwan, Taiwan
    • Chinese Culture University
      T’ai-pei, Taipei, Taiwan
  • 2009–2013
    • China Medical University (ROC)
      臺中市, Taiwan, Taiwan
    • Toko University
      T’ai-pei, Taipei, Taiwan
  • 2010–2011
    • Hungkuang University
      臺中市, Taiwan, Taiwan
  • 2008
    • Julphar School of Pharmacy
      China, Maine, United States