Publications (15)10.83 Total impact
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Article: IL-17 in cutaneous lupus erythematosus.
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ABSTRACT: Lupus erythematosus (LE) is a heterogeneous disease with broad clinical spectrum from cutaneous to visceral and systemic inflammation. IL-17 isoforms (IL-17A and IL-17F) are proinflammatory cytokines with unclear implications in lupus erythematosus pathogenesis. In this study we focused upon IL-17 in normal and modified lupus skin with a correlative study between local and serological expression. 89 subjects were recruited and divided in 5 groups-10 patients with psoriasis (disease control group), 13 healthy controls, 26 with discoid chronic lupus (DLE), 23 with systemic lupus erythematosus (SLE) and 17 with subacute lupus erythematosus (SCLE). Blood samples and skin punched-biopsy specimens were performed. Serum IL-17A, IL-17F, and IL-23 concentrations were determined by ELISA. Skin IL-17A and CD4 expression were evaluated by immunohistochemistry. Immunohistochemical expression of IL-17A was higher in DLE, SCLE and SLE patients than in negative control subjects (all p<0.05). Serum IL-17A concentrations were higher in DLE and SLE patients than in negative controls (p<0.05). Serum IL-17A levels were similar in SCLE and negative controls (p>0.05). Serum IL-17F concentrations were higher in DLE, SCLE and SLE patients than in healthy controls (all p<0.05). In DLE, SCLE, SLE patients and healthy controls we observed comparable levels of IL-23 (p>0.05). Serum anti Ro antibodies correlate with IL-17A+ lymphocytes from SCLE lesion and SLE normal skin (all p<0.05). IL-17 isoforms (IL-17A and IL-17F) are implicated in SLE but also in DLE and SCLE immunopathogenesis.European Journal of Internal Medicine 06/2010; 21(3):202-7. · 2.00 Impact Factor -
Article: Carotid Body Paraganglioma: Two Case Reports
Acta Clinica Croatica; Vol.49 No.2. -
Article: Malignant fibrothecoma: pathomorphological comments.
Morphologie et embryologie 28(2):173-5. -
Article: Extranodal pseudolymphomas. Clinico-pathological considerations on 30 cases.
Morphologie et embryologie 33(3):213-6. -
Article: Sweat gland tumors with stromal haemangiomatous component: an undescribed type of skin tumors. Report of four cases.
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ABSTRACT: An undescribed type of skin tumour, discovered in four cases, is reported. It is characterized by sweat-gland tumours (benign or malignant), with a common stromal haemangiomatous component which confers to it a peculiar pattern. Histogenetic possibilities are discussed. A collision mechanism between a pre-existent hamartomatous component (haemangioma) and a recent sweat-gland growth may be responsible for the appearance of this type and we think that this picture is more frequent but unidentified because, to our knowledge, it was not described up to the present paper.Morphologie et embryologie 31(3):209-11. -
Article: Angiocentric glioma: presentation of two cases with dissimilar histology.
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ABSTRACT: Angiocentric glioma (AG) is a recently described tumor of the brain which was included as a distinct entity in the 2007 WHO classification. To date only 26 cases have been reported in the literature. We describe two additional cases of this possibly confusing lesion of the brain. Emphasis is put on variations in the histopathological picture. The patients (20- and 55-year-old males) presented with seizures and headaches, respectively. Imaging examination showed a small cortical-subcortical tumor in each case. Both tumors were totally removed. Paraffin blocks from the two cases were examined with classical histopathology stainings and immunohistochemistry for GFAP, vimentin, EMA, neurofilament protein, synaptophysin, S100 protein, CD31, CD34, FVIII, smooth muscle actin and Ki67. The tumor proliferation was restricted around small intraparenchymal vessels. Immunohistochemistry demonstrated positivity for glial and negativity for vascular or neuronal markers. The cell shape and arrangement was different in the two cases. AG is a peculiar tumor of uncertain histogenesis but with certain glial reactivity. Histopathology is variable but restricted, for unknown reasons, to perivascular areas. Apparently, a better prognosis than for other gliomas is distinctive. Further studies are needed in order to expand the information regarding the clinical behavior and therapeutic approach of this tumor type.Clinical neuropathology 27(6):391-5. · 1.04 Impact Factor -
Article: Tumoral infiltrate after local treatment with interferon in squamous cell carcinoma.
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ABSTRACT: Using monoclonal antibodies UCHL-1 (T lymphocytes), MT-1 (pan T) and L-26 (B lymphocytes) in the study of the tumoral infiltrate after local treatment with alpha interferon (Roferon) in patients with squamous cell carcinoma of the lower lip, it was observed that: the proportion of UCHL-1 positive cells was between 30% and 80%, the proportion of MT-1 positive cells was of 85% and that of the L-26 positive cells was of 30% of all the cells in the infiltrate. In the area in which after treatment with interferon the tumoral structures had disappeared, the proportion of T lymphocytes was smaller than in the areas in which the tumoral structures were still present. The therapeutic effect of interferon is due both to the direct effects on the tumoral cell and also to the indirect effects, namely the activation of the cytotoxic T lymphocytes and of other cells in the tumoral infiltrate.Romanian journal of internal medicine = Revue roumaine de médecine interne 31(3):207-12. -
Article: Histopathological study on inflammation in cholecystoses.
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ABSTRACT: At present, according to the unanimously accepted data, cholecystoses are noninflammatory, nonlithiasic, gallbladder diseases. However the authors' experience has proved that the inflammatory process is much more frequent than it is believed and often associated also with lithiasis, a fact which, in the authors' opinion, would justify a reconsideration of this group of diseases. This study, based on histopathologic examination, was carried out in 1,630 gallbladder specimens, surgically removed. Out of these, 278 (17.05 per cent) were identified as cholecystoses; 156 out of them were cholesteroloses and 122 diverticular diseases of the gallbladder. Inflammation as a well defined morphologic process was found in 104 cases (66.67 per cent) of cholesterolosis and in 119 cases (97.54 per cent) of diverticular disease, therefore 80.21 per cent of the cases of cholecystosis examined were associated with inflammation. As regards lithiasis, it was present in 131 of the cases (46.76 per cent). The inflammatory process presented a chronic aspect with no other particular morphologic characteristics. By correlating the histopathologic data with the clinical evolutive ones, it was observed that the presence of inflammation corresponded with a clinical evolution of the disease of about three years. The authors believed that the group of cholecystoses should be reconsidered bearing in mind that inflammation is present in most of the cases and in almost half of them it is associated with lithiases. Under these conditions the sphere of chronic nonlithiasic, noninflammatory gallbladder diseases becomes considerably reduced today.Morphologie et embryologie 30(1):33-7. -
Article: Meningeal melanocytosis in a young patient--an autopsy diagnosis.
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ABSTRACT: Primary diffuse leptomeningeal melanocytosis is a very rare form of brain tumor. We report on a rapidly fatal case in an 18-year-old man presenting with symptoms and imaging features suggestive for subarachnoid hemorrhage or meningitis. The laboratory findings and imaging examination were still confusing and the diagnosis remained unclear during the patient's life. Autopsy was the cornerstone in disclosing the lesion, confirming its usefulness in the assessment of such unusual cases. The complete profile of the tumor was obtained only by histology and immunohistochemistry. Clinicians and pathologists must be aware of diagnosis difficulties in this rare disease which can represent a serious challenge in clinical practice.Clinical neuropathology 26(6):294-8. · 1.04 Impact Factor -
Article: Actualities in the histopathological interpretation of chronic hepatitis. A review of the main classifications.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 44(1-4):35-44. · 0.52 Impact Factor -
Article: Detection of human papillomaviruses in lesions of the uterine cervix.
Journal of Cellular and Molecular Medicine 5(1):96-7. · 4.13 Impact Factor -
Article: Calcifying odontogenic cyst: report of two cases and review of literature.
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ABSTRACT: The "Histological Typing of Odontogenic Tumours" (W.H.O., 1992) classified the Calcifying Odontogenic Cyst (C.O.C.) into two variants: the non-neoplastic cystic C.O.C. and the odontogenic ghost cell tumour, which is predominantly solid. We reported two cases of C.O.C.: a case with intraosseous development and another with extraosseous localisation, in the soft tissue of the alveolar area. The first case represents a cyst delimited by a squamous, non-keratinized epithelium, thickened in some areas through the accumulation of ghost cells (big pale-staining cells with a non-staining nuclear area). The connective tissue wall contains small ameloblastoma like islands. Dysplastic dentine islands, adjacente to the basal layer of the epithelium or in the connective tissue wall were also observed. The second case was a well-delimitated tumour consisting of ameloblastoma-like islands with numerous ghost cells inside. Islands of dysplastic dentine with psammomathous calcifications also exist. In certain histological sections microcystic aspects surrounded by ghost cells, dentinoid and ameloblastoma-like structures were noticed. The histochemical reaction for keratin and the immunohistochemical reaction for epithelial membrane antigen and for citokeratin were positive for ghost cells, suggesting their epithelial origin. Through this article we try to render pathologists sensitive with a particular and rare maxillary tumour.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 43(3-4):205-12. · 0.52 Impact Factor -
Article: Morphological and immunohistochemical features of tumor infiltrating lymphocytes (TIL) in carcinomas. Note I.
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ABSTRACT: Some malignant tumours like testicular seminomas and ovarian dysgerminomas or medullar carcinoma of the breast, present an unusually lympho-histiocytic (TIL) rich stroma. Many reports have concluded that the prognosis for these patients is correlated with the intensity of TIL. Recently, some analyses consider that tumour-host interactions have a significant prognostic role in many other neoplasms. The presence of TIL may be a sign of less aggressive behaviour of some epithelial neoplasm like gastro-intestinal, pulmonary, mammary, urinary or cutaneous carcinomas (Wilson et al.). The aim of this study is a morphological and immunohistochemical (IHC) characterisation of the lympho-histiocytic populations of TIL in some gastro-intestinal and mammary carcinomas. The two localisation were chosen for their different contact with the exogene antigens and their possible different type of host's immune response.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 44(1-4):191-9. · 0.52 Impact Factor -
Article: Cellular immune response in atypical tuberculosis diagnosed by PCR in paraffin embedded material.
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ABSTRACT: The aim was to evaluate the cellular immune response in atypical tuberculosis and granulomatous inflammation consistent with tuberculosis (TBC), negative histochemically for acid-fast bacilli and analysed by PCR for Mycobacterium tuberculosis (MT) detection in paraffin-embedded tissue. Thirty six samples of differently localized atypical tuberculous lesions and granulomatous tuberculoid lesions negative for acid fast bacilli and 4 positive cases on Ziehl-Nielsen stain were analysed by PCR for MT detection and were tested immunohistochemically (IHC) for the cellular immune response in the granulomas and perigranulomatous tissue. The samples selected were: 7 pulmonary and 33 extrapulmonary specimens, especially lymph nodes. Histologically, the atypical tuberculous lesions contained supurative necrosis, defective granulomas and cellular polymorphism. The epithelioid cells showed frequent mitoses. The immunoprofile of cells was polymorphous. L26 positive small lymphocytes were found in nodular lymphoid aggregates surrounding granulomas. A significantly increased number of positive UCHL1 cells were found in 33 out of the 40 analysed cases, with a larger percentage of CD4 positive T cells (81.8% of cases). CD44 was positive in multinucleated giant cells (17.5% of cases), epithelioid cells (60% of cases) and lymphocytes (30% of cases). CD68 was localized in multinucleated giant cells and epithelioid cells, in a 4%, respectively 62.5% of cases. The PCR was performed in all 40 cases; the tissue samples were heterogeneous (lung, lymph nodes, lever, nasopharynx, etc.) and needed a good quality extraction of DNA. Performing a control PCR for Beta Globin tested the extraction; a good result was obtained in 31 cases (77.5%); from these, 19 cases had amplification for IS 6110. The cellular immune response in the atypical tuberculous lesions was similar in cases with and without acid-fast bacilli, but positive for PCR. In the most cases with negative PCR reaction, it was due to a deficient fixation of the material. The T lymphocytes were numerous in all types of tuberculous granulomas, with the prominence of CD4 positive subtype. The immunoprofile of the epithelioid cells, positive for CD44 and CD68, presenting frequently mitoses suggests an activate state in a possible relationship to the T-cell-mediated immune response in tuberculosis.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 45:63-72. · 0.52 Impact Factor -
Article: Histopathological aspects of pulmonary carcinoid tumors.
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ABSTRACT: Pulmonary carcinoid tumors are considered low grade malignant tumors, arising from neuroendocrine cells from bronchial mucosa. The small cell proliferation is arranged in small nests or trabeculae, the nuclei are round to oval with finely dispersed chromatin, indistinct nucleoli, small amount of cytoplasm, indistinct borders. Problems of differential diagnosis could appear in distinction with others malignancies like adenocarcinomas, squamous cell carcinomas, lymphomas and others neuroendocrine tumors, especially in the different prognosis and therapeutic approach. We described 15 cases of pulmonary carcinoid tumors diagnosticated by morphologic and immunohistochemical methods.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 44(1-4):45-9. · 0.52 Impact Factor
