J. Abdelmoula

Hôpital Charles-Nicolle, Tunis-Ville, Tūnis, Tunisia

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Publications (63)25.67 Total impact

  • Clinical chemistry and laboratory medicine : CCLM / FESCC. 08/2014;
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    ABSTRACT: Abstract Several studies showed that organophosphorus pesticides disturb glucose homeostasis and can increase incidence of metabolic disorders and diabetes via insulin resistance. The current study investigates the influence of malathion on glucose metabolism regulation, in vivo, during subchronic exposure. Malathion was administered orally (200 mg/kg), once a day for 28 consecutive days. Plasma glucose, insulin and Glycated hemoglobin levels were significantly increased while hepatic glycogen content was decreased in intoxicated animals compared with the control group. Furthermore, there was a significant disturbance of lipid content in subchronic treated and post-treated rats deprived of malathion for one month. In addition, we used the homeostasis model assessment (HOMA) to assess insulin resistance (HOMA-IR) and pancreatic β-cell function (HOMA-β). Our results show that malathion increases insulin resistance biomarkers and decreases insulin sensitivity indices. Statistical analysis demonstrates that there was a positive and strong significant correlation between insulin level and insulin resistance indices, HOMA-IR, HOMA-β. Similarly, a negative and significant correlation was also found between insulin level and insulin sensitivity indices. For the first time, we demonstrate that malathion induces insulin resistance in vivo using homeostasis model assessment and these changes were detectable one month after the end of exposure. To explain insulin resistance induced by malathion we focus on lipid metabolism disturbances and their interaction with many proteins involved in insulin signaling pathways.
    Drug and Chemical Toxicology 07/2014; · 1.29 Impact Factor
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    ABSTRACT: Occupational exposure to organophosphate pesticides is becoming a common and increasingly alarming world-wide phenomenon. The present study is designed to investigate the preventive effect of N-acetylcysteine on malathion-induced hepatic injury and inflammation in rats. Adult male Wistar rats of body weight 200-230g were used for the study. Malathion (200mg/kg b.w./day) was administered to rats by oral intubation and N-acetylcysteine (2g/l) in drinking water for 28days. Rats were sacrificed on the 28th day, 2hours after the last administration. Markers of liver injury (aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate desyhdogenase), inflammation (leukocyte counts, myeloperoxidase, immunophenotyping of CD4+ and CD8+, interleukin-1β, interleukin-6 and interferon-γ expression) and oxidative stress (lipid peroxidation, reduced glutathione and antioxidant status) were assessed. Malathion induced an increase in activities of hepatocellular enzymes in plasma, lipid peroxidation index, CD3+/CD4+ and CD3+/CD4+ percent and pro-inflammatory cytokines, when decreased antioxidant status in liver was noted. When malathion-treated rats were compared to NAC supplemented rats, leukocytosis, T cell count and IL-1β, IL-6, INF-γ expression were reduced. Furthermore, NAC restored liver enzyme activities and oxidative stress markers. Malathion induces hepatotoxicity, oxidative stress and liver inflammation. N-acetylcysteine showed therapeutic effects against malathion toxicity.
    Life sciences 05/2014; · 2.56 Impact Factor
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    ABSTRACT: Abstract Malathion toxicity has been related to the inhibition of acetylcholinesterase, induction of oxidative stress, liver damage and impairment of kidney function as well as hematotoxicity. N-acetyl-l-cysteine (NAC) has been shown to possess curative effects in experimental and clinical investigations. The present study was designed to evaluate the protective effect of NAC against toxic consequences of malathion exposure in Wistar rats. Malathion was given daily to rats via oral gavage and NAC in drinking water during seven days. When malathion-treated rats were compared with control, a leukocytosis and reduced hemoglobin (HGB) content were detected. Furthermore, malathion produced a significant increase in liver enzymes such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and creatinine kinase. In addition, a decrease in acid phosphatase activity, protein and globulin levels were observed in malathion-treated rats compared with control. Moreover, analyses of the mineral status showed a disturbance in calcium, magnesium, phosphore and iron contents of the malathion-treated rats. Interestingly, NAC showed therapeutic effects against malathion toxicity. Indeed, HGB content and all liver enzymes were restored to normal values. Finally, the use of NAC as therapeutic agent for only seven days during malathion exposure showed interesting results on tissues damages.
    Toxicology mechanisms and methods 05/2014; 24(4):294-306. · 1.37 Impact Factor
  • La Tunisie médicale 02/2014; 92(2):187-8.
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    ABSTRACT: The cardiovascular risk appears very early in the history of diabetes mellitus and is related not only to hyperglycemia but mainly to the other risk factors associated to diabetes mellitus. To evaluate the prevalence of non-conventional cardiovascular risk factors in a Tunisian diabetic population. The prospective study enrolled 120 type 2 diabetic patients recently diagnosed and 60 healthy people, sex and age matched. All have benefited from blood sampling in order to analyze biological parameters routinely undertaken in diabetes. Non-conventional cardiovascular risk factors were also determined such as: microalbuminuria of 24hours, high sensitivity C-reactive protein (hs CRP), homocysteinemia, vitamin B12, folate and insulinemia. The participants have also benefited from abdominal echography to search nonalcoholic hepatic steatosis. Diabetics were aged 51.4±8.9 years in comparison with healthy people (50.1±6.39 years). A positive microalbuminuria was observed in 27.5% of diabetics versus 6.9% in healthy people (OR=5.1; P=0.001). The two third of diabetics had metabolic syndrome versus 25% of healthy people (OR=6.0; P<0.001) and insulinoresistance evaluated by HOMA-IR, was 3.4±0.2 in diabetics versus 2±0.1 in healthy people; P<0.001. HsCRP level was significantly higher in diabetics in comparison with healthy people (3.7±0.2mg/L versus 1.9±0.3mg/L; P<0.001) and hyperhomocysteinemia was more frequently found in diabetics. Also, we noted that 69.6% of patients had hepatic steatosis versus only 24.6% of healthy people (OR=7.1; P<0.001). The non-conventional cardiovascular risk factors were more frequently found in early diagnosed type 2 diabetic patients than in healthy people. These non-conventional factors could be helpful in stratification of the cardiovascular risk level and also in the screening of ischemic heart diseases.
    La Presse Médicale 12/2013; · 0.87 Impact Factor
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    ABSTRACT: Background The cardiovascular risk appears very early in the history of diabetes mellitus and is related not only to hyperglycemia but mainly to the other risk factors associated to diabetes mellitus. Objective To evaluate the prevalence of non-conventional cardiovascular risk factors in a Tunisian diabetic population. Patients and methods The prospective study enrolled 120 type 2 diabetic patients recently diagnosed and 60 healthy people, sex and age matched. All have benefited from blood sampling in order to analyze biological parameters routinely undertaken in diabetes. Non-conventional cardiovascular risk factors were also determined such as: microalbuminuria of 24 hours, high sensitivity C-reactive protein (hs CRP), homocysteinemia, vitamin B12, folate and insulinemia. The participants have also benefited from abdominal echography to search nonalcoholic hepatic steatosis. Results Diabetics were aged 51.4 ± 8.9 years in comparison with healthy people (50.1 ± 6.39 years). A positive microalbuminuria was observed in 27.5% of diabetics versus 6.9% in healthy people (OR = 5.1; P = 0.001). The two third of diabetics had metabolic syndrome versus 25% of healthy people (OR = 6.0; P < 0.001) and insulinoresistance evaluated by HOMA-IR, was 3.4 ± 0.2 in diabetics versus 2 ± 0.1 in healthy people; P < 0.001. HsCRP level was significantly higher in diabetics in comparison with healthy people (3.7 ± 0.2 mg/L versus 1.9 ± 0.3 mg/L; P < 0.001) and hyperhomocysteinemia was more frequently found in diabetics. Also, we noted that 69.6% of patients had hepatic steatosis versus only 24.6% of healthy people (OR = 7.1; P < 0.001). Conclusion The non-conventional cardiovascular risk factors were more frequently found in early diagnosed type 2 diabetic patients than in healthy people. These non-conventional factors could be helpful in stratification of the cardiovascular risk level and also in the screening of ischemic heart diseases. Ce qui était connu•Le diabète est responsable d’une morbi-mortalité cardiovasculaire.•Le risque cardiovasculaire est lié à l’hyperglycémie et aux autres facteurs de risque fréquemment associés au diabète.Ce qu’apporte l’étude•Bien que l’étude ait porté sur des patients dont le diabète était de découverte récente (< 5 ans), la prévalence des facteurs de risque cardiovasculaire non conventionnels était élevée (60 % avaient une CRPus augmentée et 69,6 % une stéatose hépatique).•La prise en charge optimale du diabétique repose sur une évaluation du risque cardiovasculaire global dès la découverte de la maladie.
    La Presse Médicale. 01/2013;
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    ABSTRACT: Objectifs La dégradation protéolytique de l’agrécane constitue un élément clé dans la physiopathologie de l’arthrose. Le but de cette étude a été de développer un test immunoenzymatique Elisa permettant de quantifier les taux sériques des fragments spécifiques de dégradation de l’agrécane induits par le clivage effectué par l’agrécanase. Nous avons analysé les relations entre deux fragments de dégradation de l’agrécane et les taux urinaires de C terminale du collagène de type II (CTX-II). Méthodes Notre méthode Elisa compétitive utilise un anticorps polyclonal dirigé contre les fragments d’agrécane contenant les néoépitopes NITEGE373 et 374ARGSVI. Nous avons mesuré les taux sériques de NITEGE et ARGSV chez 125 femmes atteintes d’une gonarthrose (âge 53 ± 7,6 ans, arthrose évoluant depuis 3,6 ± 3,8 ans) et chez 57 femmes témoins appariées pour l’âge. Résultats L’imprécision (CV) intra- et inter-essai des deux tests mesurant les néoépitopes de l’agrécane étaient inférieure à 20 % et ils avaient une bonne linéarité. Les valeurs médianes des taux sériques d’ARGSVI (diminution de 18 % ; p = 0,002) et de NITEGE (diminution de 36,4 % ; p < 0,001) étaient significativement diminuées chez les patientes atteints de gonarthrose par rapport aux sujets témoins. Chez les patientes atteints de gonarthrose, la hauteur minimale de l’interligne articulaire était inversement corrélée au taux d’ARGSVI (r = –0,368 ; p = 0,04) et au taux de NITEGE (r = –0,274 ; p = 0,038). La valeur médiane des taux urinaires de CTX-II était significativement augmentée de 39,5 % (p = 0,001) chez les patients atteints de gonarthrose par rapport aux sujets témoins. Conclusions Pour la première fois, les marqueurs de dégradation de l’agrécane ont été analysés dans une population arthrosique africaine. Ces marqueurs peuvent être utilisés pour apprécier l’activité de l’agrécanase dans les maladies articulaires humaines. Leur mesure, en association avec celle des CTX-II, pourrait améliorer l’évaluation clinique des patients arthrosiques.
    Revue du Rhumatisme. 10/2012; 79(5):418–424.
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    ABSTRACT: Objective To study the frequency of silent myocardial ischemia (SMI) in Tunisian patients with recent type 2 diabetes and identify cardiovascular risk factors directly in relation with SMI.Patients and methodsOne hundred and twenty diabetics and sixty healthy people have benefited from blood sampling, electrocardiogram and exercise test.ResultsThe frequency of SMI was 21% in diabetics and 3% in healthy people (P = 0.01). Obesity and hypertension were higher in diabetics than in healthy people (P = 0.001 and P < 10−4). Using unvaried analysis for risk factors with the presence of SMI in diabetics, we found that age greater than 60 yrs, male sex, sedentary and smoking were significantly correlated with SMI; respectively P = 0.004, 0.01, 0.009 and 0.03. The SMI was found in 37% of diabetics with high blood pressure vs 8% in diabetics with normal blood pressure and was correlated with hypertriglyceridemia, hypoHDLemia and microalbuminuria. Patients with SMI had at least two cardiovascular risk factors apart from diabetes among those: age greater or equal to 60 yrs, male sex, smoking, hypertension, dyslipidemia and family history of early coronaropathy. Chronic inflammation and hyperhomocysteinemia were significantly correlated to SMI; OR = 4.2 and 3.8. In addition, SMI was found in one diabetic over three who had bad glycemic control. Using multivariate analysis, only age greater or equal to 60 yrs, smoking, hypertension, hyperhomocysteinemia and hypertriglyceridemia were risk factors directly in relation with SMI in type 2 diabetes.Conclusion The assessment of global cardiovascular risk from the moment of discovering type 2 diabetes and the early screening of SMI should be necessary.
    Annales de cardiologie et d'angeiologie 04/2012; 61(2):81–87. · 0.21 Impact Factor
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    ABSTRACT: To determine the clinical, biological, and radiological futures of primary hyper-oxaluria type 1 in Tunisian children, we retrospectively studied 44 children with primary hyper-oxaluria type 1 who were treated in our center from 1995 to 2009. The diagnosis was established by quantitative urinary oxalate excretion. In patients with renal impairment, the diagnosis was made by infrared spectroscopy of stones or kidney biopsies. The male-to-female ratio was 1:2. The median age at diagnosis was 5.75 years. About 43% of the patients were diagnosed before the age of five years with initial symptoms dominated by uremia. Four patients were asymptomatic and diagnosed by sibling screenings of known patients. Nephrocalcinosis was present in all the patients; it was cortical in 34%, medullary in 32%, and global in 34%. At diagnosis, 12 (27%) children were in end-stage renal disease. Pyridoxine response, which is defined by a reduction in urine oxalate excretion of 60% or more, was obtained in 27% of the cases. In the majority of patients, the clinical expression of primary hyperoxaluria type 1 was characterized by nephrocalcinosis, urolithiasis, and renal failure; pyridoxine sensitivity was associated with better outcome.
    Saudi journal of kidney diseases and transplantation: an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia 03/2012; 23(2):385-90.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):45–46.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):39.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):46.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):34–35.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):38–39.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):34.
  • Immuno-analyse & Biologie Spécialisée. 02/2012; 27(1):35.
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    ABSTRACT: OBJECTIVES: Proteolytic degradation of aggrecan is a hallmark of the pathology of osteoarthritis. The aim of this study was to develop enzyme-linked immunosorbent assay (ELISA) to quantify the serum levels of specific aggrecan fragments generated by aggrecanases-mediated cleavage. We investigated the relationships between these two aggrecan degradations fragments and urinary CTX-II levels. METHODS: The competitive ELISAs employ a polyclonal antibody raised against the aggrecan fragments containing two neoepitopes NITEGE(373)and (374)ARGSVI. We measured serum levels of ARGSV and NITEGE in 125 women with knee osteoarthritis (mean±SD age of 53.6±7.6 years, mean±SD disease duration of 3.6±3.8 years), and 57 women age-matched controls. RESULTS: Aggrecan neoepitopes assays showed an intra- and inter-assay imprecision (CV) lower than 20% for both tests and good linearity. Median serum ARGSVI (by 18%; P=0.002), and NITEGE (36.4%; P<0.001) levels were significantly decreased in patients with knee osteoarthritis compared with controls. Minimal joint space width was negatively correlated with ARGSVI (r=-0.368, P=0.04) and NITEGE (r=-0.274, P=0.038) in knee osteoarthritis patients. Median urinary CTX-II levels were significantly increased by 39.5% (P=0.001) in knee OA patients compared with controls. CONCLUSION: Markers of degradation aggrecan were analyzed for the first time in an African osteoarthritis population. These markers can be used to monitor aggrecanase activity in human joint disease. Their combination with CTX-II can improve clinical investigation of patients with osteoarthritis patients.
    Joint, bone, spine: revue du rhumatisme 01/2012; · 2.25 Impact Factor
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    ABSTRACT: Osteoarthritis is characterized by a progressive degeneration of articular cartilage and loss of joint function. Clinical assessment of osteoarthritis is hampered by the lack of accurate measures of disease and disease progression, especially during the early stage. To investigate urinary C-telopeptide fragments of type II collagen (CTX-II) levels in knee osteoarthritis in the Tunisian population compared with controls and to assess the association between this biomarker and radiological signs. One hundred and twenty five female patients with knee osteoarthritis, aged 53.6 +/- 7.6 years with disease duration of 3.6 +/- 3.8 years and 57 female age-matched controls underwent Lyon Schuss X-ray exams. Two experienced readers independently measured the joint space width (JSW) and classified each knee for severity using the Kellgren/Lawrence scale. The urinary concentration of CTX-II was measured by a competitive ELISA. The levels of urinary CTX-II were significantly higher in knee osteoarthritis patients compared with controls (323.98 vs 218.04 microg/mol creatinine). A weak and non significant association between the CTX-II level and JSW was found. The significant correlations were observed between age and CTX-II in both groups and between BMI and CTX-II only in controls. Analysis of CTX-II in urine samples of Tunisian patients with knee osteoarthritis provided a sensitive method to detect increased degradation of collagen type II in patients with osteoarthritis.
    Clinical laboratory 01/2012; 58(3-4):209-15. · 0.92 Impact Factor
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    ABSTRACT: To study the frequency of silent myocardial ischemia (SMI) in Tunisian patients with recent type 2 diabetes and identify cardiovascular risk factors directly in relation with SMI. One hundred and twenty diabetics and sixty healthy people have benefited from blood sampling, electrocardiogram and exercise test. The frequency of SMI was 21% in diabetics and 3% in healthy people (P=0.01). Obesity and hypertension were higher in diabetics than in healthy people (P=0.001 and P<10(-4)). Using unvaried analysis for risk factors with the presence of SMI in diabetics, we found that age greater than 60 yrs, male sex, sedentary and smoking were significantly correlated with SMI; respectively P=0.004, 0.01, 0.009 and 0.03. The SMI was found in 37% of diabetics with high blood pressure vs 8% in diabetics with normal blood pressure and was correlated with hypertriglyceridemia, hypoHDLemia and microalbuminuria. Patients with SMI had at least two cardiovascular risk factors apart from diabetes among those: age greater or equal to 60 yrs, male sex, smoking, hypertension, dyslipidemia and family history of early coronaropathy. Chronic inflammation and hyperhomocysteinemia were significantly correlated to SMI; OR=4.2 and 3.8. In addition, SMI was found in one diabetic over three who had bad glycemic control. Using multivariate analysis, only age greater or equal to 60 yrs, smoking, hypertension, hyperhomocysteinemia and hypertriglyceridemia were risk factors directly in relation with SMI in type 2 diabetes. The assessment of global cardiovascular risk from the moment of discovering type 2 diabetes and the early screening of SMI should be necessary.
    Annales de cardiologie et d'angeiologie 11/2011; 61(2):81-7. · 0.21 Impact Factor