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ABSTRACT: Context and objective. The aim of the study was to investigate whether treatment with N-acetylcysteine (NAC) is able to restore erythrocyte glutathione (GSH) content in workers exposed to lead. Additionally, we measured the leukocyte and erythrocyte activities of GSH-related enzymes, such as glutathione reductase (GR), glutathione-S-transferase (GST), and glucose-6-phosphate dehydrogenase (G6PD), and estimated the influence of NAC administration on oxidative stress intensity, which was measured as the lipofuscin (LPS) level in erythrocytes. Methods. The exposed population consisted of 171 healthy males randomly divided into four groups. Workers in the first group (n = 49) were not administered any antioxidants, drugs, vitamins, or dietary supplements, while workers in the remaining groups were treated with NAC at three doses for 12 weeks (1 × 200 mg per day, 2 × 200 mg per day, and 2 × 400 mg per day). All workers continued to work during the study. The blood of all examined workers was drawn two times: at the beginning of the study and after 12 weeks of treatment. Results and conclusion. Blood lead levels decreased significantly in all groups receiving NAC compared to those in baseline. Erythrocyte GSH concentrations were significantly elevated in workers receiving 400 and 800 mg of NAC compared to those in baseline by 5% and 6%, respectively. Erythrocyte G6PD activity was significantly elevated in workers receiving 200, 400, and 800 mg of NAC compared to those in baseline by 24%, 14%, and 14%, respectively. By contrast, there were no significant differences in leukocyte G6PD or leukocyte and erythrocyte glutathione reductase (GR) activities before and after treatment. Leukocyte GST activities decreased significantly after treatment in workers receiving 200 mg of NAC by 34%, while LPS levels decreased significantly in workers receiving 200, 400, and 800 mg of NAC compared to those in baseline by 5%, 15%, and 13%, respectively. In conclusion, NAC decreases oxidative stress in workers exposed to lead via stimulating GSH synthesis.
Clinical Toxicology 06/2013; · 2.22 Impact Factor
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ABSTRACT: Lead-induced oxidative stress has been identified as the essential factor in lead poisoning pathogenesis. Therefore, the present study examined the association between occupational lead exposure and blood rheological parameters with respect to malondialdehyde (a lipid peroxidation product), lipofuscin, and glutathione concentrations in erythrocytes. The examined group included 283 healthy male employees of lead-zinc works. In brief, 129 workers were classified as the low-exposure group, while the high-exposure group was composed of 154 workers. The mean blood levels of lead and zinc-protoporphyrin and the mean urine concentrations of delta-aminolevulinic acid were used as exposure markers. The control group consisted of 73 healthy male administrative workers. Whole blood viscosity was elevated in both exposure subgroups compared with the control group. Erythrocyte aggregability increased significantly; although the increase was greater in the low exposure group. Erythrocyte deformability decreased in both subgroups. The levels of malondialdehyde and lipofuscin were significantly elevated, whereas the glutathione content decreased. In conclusion, occupational exposure to lead may induce oxidative stress in erythrocytes. This stress elevates whole blood viscosity and disturbs erythrocyte aggregability and deformability. There is a dose-effect relationship between lead levels and blood rheological parameters.
Clinical hemorheology and microcirculation 01/2013; · 3.40 Impact Factor
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ABSTRACT: The study objective was to evaluate the effect of occupational lead exposure on blood concentrations of zinc, iron, copper, selenium and proteins related to them, such as transferrin, caeruloplasmin and haptoglobin. The examined group consisted of 192 healthy male employees of zinc-lead works. By the degree of lead exposure, the exposed group was subdivided into three subgroups. The control group was composed of 73 healthy male administrative workers. The markers of lead exposure (blood levels of lead and zinc protoporphyrin) were significantly elevated in the exposed group compared with the control group. Additionally, concentrations of copper and caeruloplasmin were raised. The significant increase in haptoglobin level was observed only in the low exposure group. Selenium levels were significantly decreased, whereas iron, zinc and transferrin levels were unchanged in the exposed group compared with the control group. There were positive correlations between the lead toxicity parameters and the copper and caeruloplasmin levels. In conclusion, the effect of occupational exposure to lead on the metabolism of trace metals appears to be limited. However, significant associations between lead exposure and levels of copper and selenium were shown. Changed levels of positive acute-phase proteins, such as caeruloplasmin and haptoglobin, were also observed.
Biological trace element research 08/2012; · 1.92 Impact Factor
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ABSTRACT: In this study, we sought to understand the influence of occupational lead-exposure on the gene expression (Sod1) and activity (SOD) of superoxide dismutase, catalase and glutathione peroxidase (GPx, Gpx1) in leukocytes and erythrocytes. The study group consisted of 45 healthy male employees of a lead-zinc works and was divided into two subgroups: those with low exposure to lead (LE) and those with high exposure to lead (HE). In addition, 17 healthy male administrative workers participated in the study as the control group. The gene expression levels of both Sod1 and Gpx1 were significantly increased in the LE group as compared to the control group. By contrast, we noted only an insignificant tendency for increased gene expression of both Sod1 and Gpx1 in the HE group. The expression and activity of catalase were unchanged. Nevertheless, SOD and GPx activities in erythrocytes was significantly elevated in both examined subgroups, whereas SOD activity in leukocytes was raised only in the LE group. The results of this study led us to conclude that lead has a significant influence not only on the activities of antioxidant enzymes but also on the dose-dependent expression in their genes.
Toxicology 07/2012; 301(1-3):79-84. · 3.68 Impact Factor
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ABSTRACT: Background: Osteoarthritis is one of the most common locomotor conditions and the knee is the second most frequently affected articulation. One of therapeutic methods is viscosupplementation involving intra-articular injections of hyaluronic acid preparations. The aim of the study was to evaluate the clinical status and analyse selected biochemical blood parameters in patients with knee osteoarthritis after intra-articular injections of a hyaluronic acid preparation. Material/Methods: Blood was taken from 113 patients, 60 patients with primary and 53 patients with secondary osteoarthritis, who were administered intra-articular injections of a hyaluronic acid preparation into the affected knee, according to the schedule established in the study protocol. Concentration of lipid hydroperoxides in blood plasma was determined, along with that of malondialdehyde in erythrocytes. Plasma concentrations of ceruloplasmin, TAC, albumin, total bilirubin, and uric acid were also determined. Clinical assessment was performed using a modified HHS scale and the WOMAC questionnaire. Results: Intra-articular administration of a hyaluronic acid preparation significantly reduced pain in the knee and improved its functioning, regardless of the form of osteoarthritis. Not only clinical improvement was observed, but also beneficial changes in the blood antioxidant system. Conclusions: Comparable clinical improvement was observed in both forms of osteoarthritis of the knee. It was also beneficial changes in blood antioxidant system, especially in the group with secondary osteoarthritis.
Chirurgia narzadow ruchu i ortopedia polska 01/2012; 77:105-9.
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Beata Hapeta,
Bogdan Koczy,
Agnieszka Fitowska,
Michał Dobrakowski,
Aleksandra Kasperczyk,
Alina Ostałowska,
Tomasz Stołtny,
Wojciech Widuchowski,
Piotr Lukasik,
Ewa Birkner, Sławomir Kasperczyk
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ABSTRACT: Background: In the course of musculoskeletal system diseases, such as rheumatoid arthritis and degenerative arthritis a chronic inflammatory process develops, which deteriorates all the joint elements and leads to the movement insufficiency of a patient. In case of both of theses diseases, etiology is multi-factor and still not known thoroughly. It is suggested that in the process of degradation of a joint cartilage, active form of oxygen take part. Their excessive production contributes to oxidation imbalances in cells and an oxidative stress. Under the activity of fee radicals, among others, activation of proteolytic enzymes participating the collagen degradation starts. The aim of this work is to compare parameters characteristic a cell metabolism and protein transformations taking place in the course of the aforementioned musculoskeletal system diseases. Material/Methods: The material tested consisted of fragments of synovial membrane of a knee joint taken from 36 women suffering from rheumatoid arthritis and 24 women suffering from osteoarthritis during the procedure of knee-joint endoprothesoplastic surgery. Then the material was subject to the author's methodology of preparations of synovial membrane for biochemical markings. Results: In the group of patients suffering from rheumatoid arthritis significantly higher protein and sulfhydryl groups concentrations were achieved. Moreover, an increase of activity of manganese isoenzyme of superoxide dismutase, glutamate dehydrogenase and enzymes participating in the process of collagen degradation - prolidase and acid phosphatase was observed. Conclusions: In the course of rheumatoid arthritis a speed of cell metabolism increase, which leads to a higher intensity of protein turnover in cells.
Chirurgia narzadow ruchu i ortopedia polska 01/2012; 77:53-8.
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ABSTRACT: Background: Osteoarthritis (OA), and particularly osteoarthritis of the hip (coxarthrosis), together with hypertension and diabetes, belong to the most common lifestyle diseases. Usually the determination of a clear reason for the degenerative changes is impossible. However, the role of reactive oxygen species (ROS) that can affect the protein metabolism and thus cause the degradation of the joint connective tissue structures has been recently frequently pointed out. The aim of this study was to compare the activity of selected enzymes and albumin and protein level in the synovial membrane obtained during hip replacement surgery due to coxarthrosis. Material/Methods: The reference group consisted of patients after traumatic femoral neck fracture, without osteoarthritis in the medical record. Collected sections of the synovial membrane were prepared for biochemical tests according to the authors' methodology. Results: In the synovial membrane of patients with hip osteoarthritis significantly higher activity of superoxide dismutase (SOD) and glutamate dehydrogenase (GLDH) was observed, as well as increased activity of prolidase and lower activity of acid phosphatase activity compared with the reference group. The concentration of total protein and albumin in the synovial membrane was similar in both groups. Conclusions: Increased SOD activity indirectly indicates ROS participation in OA pathogenesis. Coexisting protein metabolism disorders of the synovial membrane may contribute to the degradation of the articular cartilage.
Chirurgia narzadow ruchu i ortopedia polska 01/2012; 77:21-6.
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ABSTRACT: The effects of administration of oxidized rapeseed oil and α-lipoic acid on activities of blood antioxidant enzymes and malondialdehyde (MDA) concentration were studied in laboratory rats fed a high-fat diet. Addition of oxidized oil resulted in increased production of oxygen radicals, evidenced by elevated plasma MDA production. Such effect was counteracted by administration of α-lipoic acid. There was an increase of the activities of superoxide dismutase (total and Cu/Zn-SOD) and catalase in rats fed a high-fat diet to which 10% oxidized oil was added. Administration of α-lipoic acid resulted in a decrease of the activities of these enzymes.
Biological trace element research 09/2011; 145(2):217-21. · 1.92 Impact Factor
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ABSTRACT: Lead plays a significant role in modern industry. This metal is related to a broad range of physiological, biochemical and behavioural dysfunctions. The genotoxic effects of lead have been studied both in animals and humans in in vitro systems but results were contradictory. The aim of this study was to investigate the association between DNA damage and occupational exposure to lead in workers. The study population consisted of 62 employees of metalworks exposed to lead in the southern region of Poland. The control group consisted of 26 office workers with no history of occupational exposure to lead. The concentration of lead (PbB) and zincprotoporphyrin (ZPP) in blood samples were measured. The DNA damage was analyzed in blood lymphocytes using alkaline comet assay. The level of DNA damage was determined as the percentage of DNA in the tail, tail length and tail moment. The lead exposure indicators were significantly higher in lead exposed group: PbB about 8.5 times and ZPP 3.3 times. Also, the percentage of DNA in the tail (60.3 ± 14 vs. 37.1 ± 17.6), comet tail length (86.9 ± 15.49 vs. 73.8 ± 19.12) and TM (57.8 ± 17.82 vs. 33.2 ± 19.13) were significantly higher in the study group when compared with the controls; however, the difference between the subgroups was only 5-10%. Years of lead exposure positively correlated with all comet assay parameters (R = 0.21-0.41). Both mean and current PbB and ZPP were correlated with tail DNA % and TM (R = 0.32; R = 0.33; R = 0.24; R = 0.26 and R = 0.34; R = 0.33; R = 0.28 and R = 0.28, respectively). This study shows that occupational exposure to lead is associated with DNA damage and confirmed that comet assay is a rapid, sensitive method suitable for biomonitoring studies.
Annals of agricultural and environmental medicine: AAEM 12/2010; 17(2):231-6. · 2.31 Impact Factor
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ABSTRACT: The aim of the study was to evaluate the antioxidant status in plasma and erythrocytes from patients with ankylosing spondylitis (AS).
16 patients with AS and 16 healthy volunteers were involved in this study. Activities of antioxidant enzymes: superoxide dismutase (SOD) and its isoenzymes - (SOD-Mn) and (SOD-ZnCu), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione transferase (GST), as well as the total antioxidant status (TAS) and concentration of malondialdehyde (MDA) in plasma and/or erythrocytes, respectively were determined.
In patients with AS, a statistically significant decrease in plasma activity of SOD, SOD-CuZn and TAS, significant drop in activity of SOD, GPx, GST and GR in erythrocytes, as well as increased concentration of MDA in comparison with control group of healthy volunteers was observed.
Decrease in antioxidant status leading to generation of oxidative stress may play an important role in the pathogenesis of ankylosing spondylitis.
Clinical biochemistry 04/2010; 43(6):566-70. · 2.02 Impact Factor
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ABSTRACT: Oxidative stress is a common mechanism by which chemical toxicity can occur in the liver. The aim of the studies conducted has been to determine what influence the administration of methionine during intoxication with sodium fluoride may have upon the selected enzymes of the antioxidative system in rat liver. The experiment was carried out on Wistar FL rats (adult females) that, for 35 days, were administered distilled water, NaF, or NaF with methionine (doses: 10 mg NaF/kg bw/day, 10 mg Met/kg bw/day). The influence of administered NaF and Met was examined by analyzing the activity of the antioxidative enzymes: superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione transferase in the liver. The results suggest that fluoride reduces the efficiency of the enzymatic antioxidative system in the liver. Administration of methionine during intoxication with sodium fluoride does not have an advantageous influence upon the activity of superoxide dismutase, catalase, reductase, and glutathione transferase in the liver. The slight increase of the activity of glutathione peroxidase after administration of methionine may indicate its protective influence upon that enzyme.
Biological trace element research 03/2010; 139(3):325-31. · 1.92 Impact Factor
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ABSTRACT: The effects of administration of oxidized rapeseed oil and α-lipoic acid on activities of hepatic antioxidant enzymes and lipid peroxidation were studied in laboratory rats. There was an increase of the activities of superoxide dismutase, glutathione peroxidase, glutathione reductase, and glucose-6-phosphate dehydrogenase in rats fed a high fat diet to which 10% oxidized oil was added. Administration of α-lipoic acid resulted in a decrease of the activities of these enzymes. Addition of oxidized oil also resulted in increased production of oxygen radicals, evidenced by elevated malondialdehyde production. Such effect was counteracted by administration of α-lipoic acid.
Biological trace element research 02/2010; 138(1-3):272-81. · 1.92 Impact Factor
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ABSTRACT: Even though heterotopic ossification (HO) is most often asymptomatic in patients after total hip replacement (THR), it remains a serious problem in orthopaedics as it is observed in nearly all operated patients.
The article presents a pre- and post-operative retrospective analysis of heterotopic ossification based on radiographic evidence. The study involved 77 patients below forty years old who underwent THR due to degenerative changes. Heterotopic ossification was evaluated using Brooker's basic four-grade scale.
Heterotopic ossification was diagnosed more often in women. The study confirmed once again that non-steroidal anti-inflammatory drug (NSAID) prophylaxis reduces the risk of ossification. We propose that the hydroxyapatite coating of implants may also contribute to a higher incidence of heterotopic ossification in operated patients.
A history of surgical procedures and a lack of NSAID prophylaxis contributed to heterotopic ossification in the region of the replaced hip joint in the study group.
Ortopedia, traumatologia, rehabilitacja 10/2009; 11(5):458-66.
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ABSTRACT: The intensified or uncontrolled formation of reactive oxygen species leads to disturbances of numerous biochemical processes. Among the factors inducing intensified free radical processes, fluoride ions are listed, among others. One of the organs most exposed to the toxic activity of fluorides is the kidney. In the study presented here, the influence of fluorine upon the activity of selected antioxidant enzymes in rat kidney has been examined, as well as antioxidant properties of methionine during intoxication with sodium fluoride. The experiment was carried out on Wistar FL rats (adult females) that for 35 days were administered water, NaF, NaF with methionine (doses: 10 mg NaF/kg bw/day, 10 mg Met/kg bw/day) . The influence of administered NaF and Met upon the antioxidative system in kidney was examined by analyzing the activity of the most important antioxidative enzymes (SOD, CAT, GPX, GR, GST). The studies carried out confirmed the disadvantageous effect of NaF upon the antioxidative system in rats (decrease in activity of antioxidative enzymes). Methionine increased the activity of antioxidative enzymes, most efficiently that of GPX, GR, and GST.
Biological trace element research 07/2009; 133(1):60-70. · 1.92 Impact Factor
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ABSTRACT: Two experiments have been carried out, each on 18 (male) rabbits of the New Zealand breed. In each of them, animals were divided into three groups of six: control group, cholesterol group (CH), and cholesterol + fluoride group (CH+F). Experimental hypercholesterolemia has been induced in the animals with the diet enriched with 0.5 and 2 g% of cholesterol/100 g of fodder/24 h. The rabbits from CH+F groups have also been administered fluoride ions contained in drinking water (3 mg F(-)/kg of body mass/24 h). The influence of fluoride ions upon the concentrations of malondialdehyde (MDA) and activity of antioxidative enzymes, superoxide dismutase (SOD), mitochondrial enzyme (MnSOD), cytoplasmatic enzyme (ZnCuSOD), and glutathione peroxidase (GPX), has been examined in liver of rabbits. An increase (in comparison with cholesterol groups) in the concentration of MDA in both (CH+F) groups in rabbit liver has been noted. Moreover, a decrease (statistically significant) of SOD and MnSOD has been found in cholesterol groups, as well as in groups (CH+F) in comparison with control group. Furthermore, a decrease in the activity of SOD under the influence of F(-) together with increased activity of MnSOD (statistically significant in comparison with cholesterol groups) have been observed. The activity of ZnCuSOD increased in statistically significant manner in (CH) groups vs control group and decreased (statistically significantly in relation to cholesterol groups) under the influence of F(-).
Biological trace element research 02/2009; 129(1-3):137-42. · 1.92 Impact Factor
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ABSTRACT: The aim of the study has been to determine the influence upon the kidney, liver, and the blood prooxidative system, exercised by administration of methionine (Met), under conditions of oxidative stress induced by sodium fluoride (NaF).The experiment was carried out on Wistar FL rats (adult females) that, for 35 days, were administered distilled water, NaF or NaF with methionine (doses: 10 mg NaF/kg bw/day, 10 mg Met/kg bw/day). The influence of administered NaF and Met was examined by analyzing the concentration of malondialdehyde (MDA) in kidney, liver, erythrocytes, and blood plasma.The study confirmed the disadvantageous effect of NaF upon the antioxidative system in rats (an increase in the concentration of MDA).The administration of methionine reduced the process of lipid peroxidation (a decreased in the concentration of MDA). The best antioxidative properties have been demonstrated by methionine in rat liver.
Biological trace element research 02/2009; 129(1-3):229-38. · 1.92 Impact Factor
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ABSTRACT: The study population included employees of metal works, with significant exposure to lead (Pb) for about 20 years (mean blood lead level PbB = 43 microg/dl), divided into four groups: normotensive (Pb-normotensive), high-normotensive, first (HT-1), and second degree (HT-2) of hypertension. The control group comprised of 30 office workers with normal blood pressure and no history of occupational exposure to lead. In erythrocytes, the activity of antioxidant enzymes and lipid peroxidation (measured as concentration of malondialdehyde (MDA)) was estimated. MDA concentration, glutathione peroxide (GPx), and superoxide dimutase (SOD) activities were significantly higher in Pb-normotensive group when compared to the normotensive control. Body mass index, age, duration of exposure to lead, and PbB were higher in both hypertensive groups than in Pb-normotensive or high-normotensive groups. MDA increased in HT-1 group by 48% and in HT-2 by 72%, and the activity of GPx decreased significantly in HT-1 group, by 30% and in HT-2 by 43%. No significant differences were observed in their activity of SOD, catalase, and glutathione reductase in erythrocytes. Arterial blood pressure (both systolic and diastolic) positively correlated with body mass index (BMI), age, lead exposure duration, PbB, MDA, and negatively correlated with GPx. There was no significant correlation between BMI and MDA, BMI and GPx, age and MDA, AND age and GPx. In conclusion: (1) lead increases erythrocyte MDA concentration and the activity of GPx as well as SOD in normotensive subjects. (2) Among individuals exposed to lead, with arterial hypertension diagnosed, higher body mass index, age, values of blood lead level, and prolonged exposure to lead have been noticed, accompanied by intensified oxidative stress and the decrease in the activity of glutathione peroxidase in erythrocytes. The reasons for increase of blood pressure in lead exposure remain unrecognized.
Biological trace element research 02/2009; 130(2):95-106. · 1.92 Impact Factor
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ABSTRACT: The study population included healthy, fertile men, employees of Zinc and Lead Metalworks (n=63). Workers exposed to lead were divided into two groups: a group with moderate exposure to lead (ME) - blood lead level (PbB) 25-40 microg/dl and a group with high exposure to lead (HE) PbB=40-81 microg/dl. The control group consisted of office workers with no history of occupational exposure to lead. Evaluation of lead, cadmium and zinc level in blood and seminal plasma, zinc protoporphyrin in blood (ZPP), 5-aminolevulinic acid in urine (ALA), malondialdehyde (MDA) in seminal plasma and sperm analysis were performed. No differences were noted in the concentration of cadmium and zinc in blood and seminal plasma in the study population. Lipid peroxidation in seminal plasma, represented as MDA concentration, significantly increased by about 56% in the HE group and the percentage of motile sperm cells after 1 h decreased by about 34% in comparison to the control group. No statistically significant correlation between other parameters of sperm analysis and lead exposure parameters nor between lead, cadmium and zinc concentration in blood and seminal plasma were found. A positive association between lead intoxication parameters (PbB, ZPP, lead seminal plasma) and MDA concentration in sperm plasma and inverse correlation with sperm cells motility (PbB, ZPP) was found. An increased concentration of MDA was accompanied by a drop in sperm cells motility. In conclusion, we report that high exposure to lead causes a decrease of sperm motility in men most likely as a result of increased lipid peroxidation, especially if the level in the blood surpasses the concentration of 40 microg/dl.
Toxicology and Applied Pharmacology 06/2008; 228(3):378-84. · 4.45 Impact Factor
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ABSTRACT: Three-month studies were performed on 18 adult rabbits of New Zealand breed divided into three groups, with six animals in each: a control group on standard diet, a cholesterol group receiving 500 mg of cholesterol/100 g of feed per rabbit per 24 h (CH group), and a cholesterol + fluorine group (CH + F group) receiving 500 mg of cholesterol/100 g of feed per rabbit per 24 h and 3 mg of F(-)/kg of body weight per 24 h. The conducted studies proved that cholesterol in the applied dosage (500 mg cholesterol per rabbit per 24 h) has an atherogenic action. Fluoride ions administered together with a 500-mg cholesterol atherogenic diet inhibit the atheromatosic changes in the aorta. The concentration of plasma cholesterol was elevated in both study groups when compared to the control group but decreased in the CH + F group when compare to the CH group. The influence of fluoride ions has been examined upon the activity of alanine aminotransferase, aspartate aminotransferase, and glutamate dehydrogenase (GLDH) in the plasma in the liver of rabbits in the course of experimental hypercholesterolemia. Increase in the activity of study enzymes has been observed in the blood plasma, which may be due to damage occurring to hepatocytes of the animals examined (a statistically significant increase in the activity of GLDH in the plasma). In the liver, the inhibition of activity for all examined enzymes has been observed in the group of rabbits with hypercholesterolemia, which testifies the disturbances in protein metabolism in examined animals. The addition of sodium fluoride to the diet rich in cholesterol results in "removing the block" on those activities, which increase. We suppose that the permeability of the hepatocyte membrane was elevated, so the activities of examined enzymes increased in the plasma ("escape" to plasma). On the one hand, fluoride ions result in probable lesion of hepatocytes membranes; on the other hand, they inhibit the atheromatosic changes in the aorta.
Biological Trace Element Research 06/2008; 124(2):118-28. · 1.92 Impact Factor
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ABSTRACT: The aim of the study has been to determine and compare the influence upon the kidney antioxidative system, exercised by administration of vitamin E, and vitamin E in combination with methionine, under conditions of oxidative stress induced by sodium fluoride. The experiment was carried out on Wistar FL rats (adult males) that, for 35 days, were administered water, NaF, NaF with vitamin E, or vitamin E with methionine (doses: 10 mg NaF/kg of body mass/24 h, 3 mg vitamin E per 10 microl per rat for 24 h, 2 mg methionine per rat for 24 h). The influence of administered sodium fluoride and antioxidants upon the antioxidative system in kidney was examined by analyzing the concentration of malondialdehyde (MDA) and the activity of the most important antioxidative enzymes (SOD, total and both its isoenzymes, GPX, GST, GR, and CAT). The studies carried out confirmed the disadvantageous effect of the administered dose of NaF upon the antixodiative system in rats (increase in the concentration MDA, decrease activity of all antioxidative enzymes). The administration of vitamin E increased the activity of studied enzymes with the exception of glutathione reductase GR; it also reduced the processes of lipid peroxidation. It has been found that combined doses of vitamin E and methionine were most effective in inhibiting lipid peroxidation processes. The results confirmed the antioxidative properties of methionine.
Biological Trace Element Research 02/2008; 121(1):51-9. · 1.92 Impact Factor
