Grégory Moureau

Publications (8)29.13 Total impact

• Article: Isolation and characterization of a new strain of lymphocytic choriomeningitis virus from rodents in southwestern france.

  Ines N Yama, Benoite Cazaux, Janice Britton-Davidian, Grégory Moureau, Laurence Thirion, Xavier de Lamballerie, Gauthier Dobigny, Rémi N Charrel
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  ABSTRACT: Abstract A total of 821 tissue samples from rodents trapped during field campaigns organized in Europe and Africa were screened for the presence of arenaviruses by molecular methods and cell culture inoculation when feasible. Two Mus musculus domesticus trapped in the southwestern part of France were infected with a potentially new strain of lymphocytic choriomeningitis virus (LCMV), here referred to as LCMV strain HP65-2009, which was isolated and genetically characterized by whole genome sequencing. Genetic and phylogenetic analyses comparing LCMV HP65-2009 with 26 other LCMV strains showed that it represents a novel highly-divergent strain within the group of Mus musculus-associated LCMV.
  Vector borne and zoonotic diseases (Larchmont, N.Y.) 05/2012; 12(10):893-903. · 2.61 Impact Factor
• Article: Punique virus, a novel phlebovirus, related to sandfly fever Naples virus, isolated from sandflies collected in Tunisia.

  Elyes Zhioua, Grégory Moureau, Ifhem Chelbi, Laetitia Ninove, Laurence Bichaud, Mohamed Derbali, Mylène Champs, Saifeddine Cherni, Nicolas Salez, Shelley Cook, Xavier de Lamballerie, Remi N Charrel
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  ABSTRACT: Sandflies are widely distributed around the Mediterranean Basin. Therefore, human populations in this area are potentially exposed to sandfly-transmitted diseases, including those caused by phleboviruses. Whilst there are substantial data in countries located in the northern part of the Mediterranean basin, few data are available for North Africa. In this study, a total of 1489 sandflies were collected in 2008 in Tunisia from two sites, bioclimatically distinct, located 235 km apart, and identified morphologically. Sandfly species comprised Phlebotomus perniciosus (52.2%), Phlebotomus longicuspis (30.1%), Phlebotomus papatasi (12.0%), Phlebotomus perfiliewi (4.6%), Phlebotomus langeroni (0.4%) and Sergentomyia minuta (0.5%). PCR screening, using generic primers for the genus Phlebovirus, resulted in the detection of ten positive pools. Sequence analysis revealed that two pools contained viral RNA corresponding to a novel virus closely related to sandfly fever Naples virus. Virus isolation in Vero cells was achieved from one pool. Genetic and phylogenetic characterization based on sequences in the three genomic segments showed that it was a novel virus distinct from other recognized members of the species. This novel virus was provisionally named Punique virus. Viral sequences in the polymerase gene corresponding to another phlebovirus closely related to but distinct from sandfly fever Sicilian virus were obtained from the eight remaining positive pools.
  Journal of General Virology 05/2010; 91(Pt 5):1275-83. · 3.36 Impact Factor
• Source

  Available from: Remi Charrel

  Article: Molecular and serological evidence for the presence of novel phleboviruses in sandflies from northern algeria.

  Grégory Moureau, Laurence Bichaud, Nicolas Salez, Laetitia Ninove, Boussad Hamrioui, Smail Belazzoug, Xavier de Lamballerie, Arezki Izri, Rémi N Charrel
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  ABSTRACT: During summer 2007, a total of 785 phlebotomine flies were trapped in northern Algeria, identified morphologically, organised as monospecific pools and tested for the presence of phlebovirus RNA using degenerate primers. Three pools were positive, and the corresponding PCR products were cloned and sequenced. Viral sequences corresponding to two phleboviruses distinct from each other were detected in sandflies circulating in two close locations (140 km apart) in Northern Algeria. The 3 sequences were aligned with homologous polymerase sequences retrieved from the Genbank database, in order to examine their phylogenetic relationships. One viral sequence (from Phlebotomus papatasi) was closely related to but distinct from a sequence obtained from Phlebotomus ariasi sandflies trapped in Algeria in 2006. The two other viral sequences (from Phlebotomus longicuspis) were genetically distantly related to sequences corresponding to virus members of the Sandfly fever Naples virus species and although falling within the same group, this clearly represents a second distinct novel lineage. These results are indicative of a high genetic heterogeneity within sandflies trapped in a relatively small geographic area. Seroprevalence studies conducted on sera from populations living in the same areas indicated that humans can be infected by these viruses.
  The Open Virology Journal 01/2010; 4:15-21.
• Source

  Available from: Mario Milani

  Article: Structure and functionality in flavivirus NS-proteins: perspectives for drug design.

  Michela Bollati, Karin Alvarez, René Assenberg, Cécile Baronti, Bruno Canard, Shelley Cook, Bruno Coutard, Etienne Decroly, Xavier de Lamballerie, Ernest A Gould, [......], Grégory Moureau, Johan Neyts, Raymond J Owens, Jingshan Ren, Barbara Selisko, Silvia Speroni, Holger Steuber, David I Stuart, Torsten Unge, Martino Bolognesi
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  ABSTRACT: Flaviviridae are small enveloped viruses hosting a positive-sense single-stranded RNA genome. Besides yellow fever virus, a landmark case in the history of virology, members of the Flavivirus genus, such as West Nile virus and dengue virus, are increasingly gaining attention due to their re-emergence and incidence in different areas of the world. Additional environmental and demographic considerations suggest that novel or known flaviviruses will continue to emerge in the future. Nevertheless, up to few years ago flaviviruses were considered low interest candidates for drug design. At the start of the European Union VIZIER Project, in 2004, just two crystal structures of protein domains from the flaviviral replication machinery were known. Such pioneering studies, however, indicated the flaviviral replication complex as a promising target for the development of antiviral compounds. Here we review structural and functional aspects emerging from the characterization of two main components (NS3 and NS5 proteins) of the flavivirus replication complex. Most of the reviewed results were achieved within the European Union VIZIER Project, and cover topics that span from viral genomics to structural biology and inhibition mechanisms. The ultimate aim of the reported approaches is to shed light on the design and development of antiviral drug leads.
  Antiviral research 11/2009; 87(2):125-48. · 3.61 Impact Factor
• Source

  Available from: Remi Charrel

  Article: Concurrent chikungunya and dengue virus infections during simultaneous outbreaks, Gabon, 2007.

  Eric M Leroy, Dieudoné Nkoghe, Benjamin Ollomo, Chimène Nze-Nkogue, Pierre Becquart, Gilda Grard, Xavier Pourrut, Rémi Charrel, Grégory Moureau, Angélique Ndjoyi-Mbiguino, Xavier De-Lamballerie
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  ABSTRACT: An outbreak of febrile illness occurred in Gabon in 2007, with 20,000 suspected cases. Chikungunya or dengue-2 virus infections were identified in 321 patients; 8 patients had documented co-infections. Aedes albopictus was identified as the principal vector for the transmission of both viruses.
  Emerging Infectious Diseases 05/2009; 15(4):591-3. · 6.79 Impact Factor
• Article: Massilia virus, a novel Phlebovirus (Bunyaviridae) isolated from sandflies in the Mediterranean.

  Rémi N Charrel, Grégory Moureau, Sarah Temmam, Arezki Izri, Pierre Marty, Philippe Parola, Amelia Travassos da Rosa, Robert B Tesh, Xavier de Lamballerie
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  ABSTRACT: A new virus was isolated from three independent pools of Phlebotomus perniciosus sandflies (Diptera; Psychodidae) trapped in two regions of southeastern France, located 90 miles apart. Microscopic, antigenic and genetic analyses indicate that this novel virus belongs to the genus Phlebovirus in the family Bunyaviridae. The new virus is designated Massilia virus since the first isolate was obtained from sandflies collected in the suburban area of Marseille. The complete genome sequence was determined and used to compare the genetic and phylogenetic relationships of Massilia virus with other phleboviruses. Genetic and antigenic properties were employed to address whether or not Massilia virus should be considered a new species within the genus, or a member of a previously recognized species. Cerebrospinal fluid specimens, collected from local patients with central nervous system infections during the previous four-year period were tested for the presence of Massilia virus RNA, but gave negative results. In conclusion, Massilia virus is proposed as a member of the Sandfly fever Naples virus complex; its public health importance has yet to be determined.
  Vector borne and zoonotic diseases (Larchmont, N.Y.) 01/2009; 9(5):519-30. · 2.61 Impact Factor
• Source

  Available from: Remi Charrel

  Article: Sandfly fever Sicilian virus, Algeria.

  Arezki Izri, Sarah Temmam, Grégory Moureau, Boussad Hamrioui, Xavier de Lamballerie, Rémi N Charrel
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  ABSTRACT: To determine whether sandfly fever Sicilian virus (SFSV) is present in Algeria, we tested sandflies for phlebovirus RNA. A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly. Of 60 human serum samples, 3 contained immunoglobulin G against SFSV. These data suggest SFSV is present in Algeria.
  Emerging Infectious Diseases 06/2008; 14(5):795-7. · 6.79 Impact Factor
• Source

  Available from: Jean-Paul Joseph Gonzalez

  Article: Genetic characterization of tick-borne flaviviruses: new insights into evolution, pathogenetic determinants and taxonomy.

  Gilda Grard, Grégory Moureau, Rémi N Charrel, Jean-Jacques Lemasson, Jean-Paul Gonzalez, Pierre Gallian, Tamara S Gritsun, Edward C Holmes, Ernest A Gould, Xavier de Lamballerie
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  ABSTRACT: Here, we analyze the complete coding sequences of all recognized tick-borne flavivirus species, including Gadgets Gully, Royal Farm and Karshi virus, seabird-associated flaviviruses, Kadam virus and previously uncharacterized isolates of Kyasanur Forest disease virus and Omsk hemorrhagic fever virus. Significant taxonomic improvements are proposed, e.g. the identification of three major groups (mammalian, seabird and Kadam tick-borne flavivirus groups), the creation of a new species (Karshi virus) and the assignment of Tick-borne encephalitis and Louping ill viruses to a unique species (Tick-borne encephalitis virus) including four viral types (i.e. Western Tick-borne encephalitis virus, Eastern Tick-borne encephalitis virus, Turkish sheep Tick-borne encephalitis virus and Louping ill Tick-borne encephalitis virus). The analyses also suggest a complex relationship between viruses infecting birds and those infecting mammals. Ticks that feed on both categories of vertebrates may constitute the evolutionary bridge between the three distinct identified lineages.
  Virology 05/2007; 361(1):80-92. · 3.35 Impact Factor