-
[show abstract]
[hide abstract]
ABSTRACT: Chemokine ligand 20 (CCL20) plays an important role in the carcinogenesis, invasion, and progression of malignancies. This study aimed to investigate the prognostic significance of CCL20 in patients with hepatocellular carcinoma (HCC) after curative resection.
Expression of CCL20 was evaluated by immunohistochemistry in tumor tissue from 125 patients who underwent curative resection of HCC. The relationship between CCL20 expression and clinicopathologic features was analyzed. Univariate and multivariate analyses were performed to evaluate its predictive value for tumor recurrence and survival of HCC patients.
CCL20 expression was verified in all tumor specimens. CCL20 expression was associated with tumor size (P = 0.002), tumor number (P = 0.031), vascular invasion (P = 0.003), tumor differentiation (P = 0.024), and tumor recurrence (P < 0.001). Patients with high CCL20 expression had poorer recurrence-free survival and overall survival (both P < 0.001) than those with low CCL20 expression. Multivariate analysis showed CCL20 expression was an independent predictor of tumor recurrence (Hazard ratio 3.934, P = 0.002), recurrence-free survival (Hazard ratio 2.573, P = 0.001), and overall survival (Hazard ratio 2.930, P = 0.001).
CCL20 expression was associated with tumor recurrence and survival of HCC patients. It may be used to predict prognosis of patients with HCC and may be a new target of postoperative adjuvant therapy.
Journal of Gastrointestinal Surgery 11/2011; 16(4):828-36. · 2.83 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A new subset of Treg cells, CD4(+) CD69(+) CD25(-) T cells, has been identified in mice. Herein, we aimed to identify this subset of T cells and to evaluate its function in patients with hepatocellular carcinoma (HCC).
We detected CD4(+) CD69(+) CD25(-) T cells and its expression of CCR6 and transforming growth factor-β1 (TGF-β1) in peripheral blood of 91 HCC patients, 38 chronic hepatitis patients and 34 healthy donors by flow cytometry. CD4(+) CD69(+) CD25(-) T cells in HCC tissues were also analyzed.
CD4(+) CD69(+) CD25(-) T cells were significantly increased in peripheral blood of HCC patients compared with healthy persons and chronic hepatitis patients (8.74% ± 0.42% vs 4.55% ± 0.33% and 5.15% ± 0.36%, P < 0.0001). The percentage of peripheral CD4(+) CD69(+) CD25(-) T cells was significantly higher in HCC patients with Tumor Node Metastasis (TNM) stage III plus IV (P < 0.05). Patients with large tumor size and tumor vascular invasion were inclined to obtain high percentage of CD4(+) CD69(+) CD25(-) T cells (P < 0.05). The frequency of membrane-bound TGF-β1 positive cells in CD4(+) CD69(+) CD25(-) T cells from HCC patients was higher than that from the other two groups (P < 0.0001). A considerable proportion of CD4(+) CD69(+) CD25(-) T cells were present in HCC tissues, which has significant correlation with tumor size and TNM stage. Few CD4(+) CD69(+) CD25(-) T cells express CCR6 both in peripheral blood and tumor tissues from HCC patients.
Increased CD4(+) CD69(+) CD25(-) T cells in HCC patients are significantly correlated with tumor size, vascular invasion and TNM stage. Thus, increased CD4(+) CD69(+) CD25(-) T cells exert a critical role in HCC progression and might be a clinically aggressive phenotype of HCC.
Journal of Gastroenterology and Hepatology 05/2011; 26(10):1519-26. · 2.87 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The aim of this study was to investigate the expression of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and its effects on tumor angiogenesis and relapse-free survival (RFS) after curative resection of hepatocellular carcinoma (HCC).
Expression of CEACAM1 and CD34 was immunohistochemically detected in HCC specimens from 97 patients. Microvessel density (MVD) was determined by counting CD34-positive endothelial cells. Statistical analyses were performed to determine the effects of CEACAM1 on clinicopathologic factors, tumor angiogenesis, and RFS.
CEACAM1 expression was detected in 91 HCC specimens; 53 cases showed membranous expression and 38 cases showed cytoplastic expression. CEACAM1 cytoplastic expression was significantly associated with tumor size, number of tumors, vascular invasion, satellite nodules, Edmondson-Steiner grade, TNM stage, and MVD (p < 0.05 for all). Moreover, CEACAM1 cytoplastic expression was significantly associated with poorer RFS. The 3-year RFS of patients with CEACAM1 cytoplastic expression was significantly lower than that of those with CEACAM1 membranous expression (26.3 vs. 52.8%, p = 0.005). Cox analysis revealed that CEACAM1 cytoplastic expression was an independent prognostic factor for 3-year RFS (p = 0.031).
CEACAM1 expression was common in HCC, and CEACAM1 cytoplastic expression was closely associated with tumor progression, angiogenesis, and poorer RFS, indicating that cytoplastic CEACAM1 might be a predictor of relapsing phenotype and a possible novel target of antiangiogenic therapy for patients with HCC.
World Journal of Surgery 04/2011; 35(10):2259-65. · 2.36 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In recent years laparoscopic proctocolectomy with ileal pouch-anal anastomosis has been used as an alternative to conventional open techniques. However, many published series on proctectomy and ileal pouch-anal anastomosis are based on open experience. This paper presents our experience of laparoscopic proctocolectomy with ileal pouch-anal anastomosis to 23 patients with ulcerative colitis and familial adenomatous polyposis. In operations only sample exteriorization and pouch formation were performed using a small left flank incision of about 4 cm, all other steps were performed entirely laparoscopically. None of the laparoscopic procedures required conversion to an open operation, and there were no intraoperative complications. The median operative time was 315 minutes (240 to 460 min), the average blood loss was 130 mL (70 to 270 mL). Postoperative pain was minimal and no patients required analgesic drugs. Bowel function returned in a median of 2 days (1 to 3 d). Postoperative complications were encountered in 5 patients 22%). No patient required surgical reintervention. The median hospital stay was 9 days (7 to 16 d). In conclusion, laparoscopic proctocolectomy with ileal pouch-anal anastomosis is technically feasible and safe. The technique described in this study provides some potential advantages such as improved cosmetic result and less blood loss. It can be used in patients with familial adenomatous polyposis and ulcerative colitis.
Surgical laparoscopy, endoscopy & percutaneous techniques 11/2007; 17(5):388-91. · 1.23 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In recent years laparoscopic proctocolectomy with ileal pouch-anal anastomosis has been used as an alternative to conventional open techniques. Since many published series on proctectomy and ileal pouch-anal anastomosis are based on open experience, in this paper we compare our laparoscopic experience on 21 patients with ulcerative colitis (UC) or familial adenomatous polyposis (FAP) to 25 patients who had undergone open proctocolectomy with ileal pouch-anal anastomosis. The median operative time in the laparoscopic group was significantly longer than that in the open group (325 min vs. 220 min). However, blood loss was less (115 ml vs.240 ml), bowel function returned earlier (2 days vs.4 days), and hospitalization time was shorter (9 days vs.11 days) in the laparoscopic group (all p<0.05). Early postoperative complications were encountered in five patients of the laparoscopic group and in seven patients of the open group. The median follow-up time was 15 months (range 6-34) in both groups. Late postoperative complications were encountered in three patients of the laparoscopic group and in three patients of the open group. In conclusion, laparoscopic proctocolectomy with ileal pouch-anal anastomosis is technically feasible. The technique described in this study provides the advantages of less blood loss, shorter hospitalization, quicker return of bowel function and more favorable cosmetic results when compared to the open technique.
Minimally Invasive Therapy & Allied Technologies 01/2007; 16(3):187-91. · 0.94 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Multihop is the main communication style for wireless sensor networks. MAC protocol has great impact on performance of network. CSMA-CA's backoff algorithm specification in the IEEE 802.15.4 standard cannot adapt to dynamic network and multihop communication. This paper proposes a strategy that relays data should have priority. This strategy guarantees that remote node's packets are transmitted reliably to destination node, whether network is burst flow or non-burst flow. A new backoff algorithm that can adapt to flow and avoid second-collision is proposed and analyzed in this paper. The paper models the networking using the theory of discrete tune Markov chains. Numerical results and OPNET simulation show that the new algorithm can adapt to burst flow better and produces throughput improvements, whether the number of node is large or small. Simulation results also show that the new algorithm relays more remote node's packets to destination node and saves energy through reducing retransmission in multihop networks
Mobile Adhoc and Sensor Systems Conference, 2005. IEEE International Conference on; 12/2005
-
[show abstract]
[hide abstract]
ABSTRACT: A novel microwave electrode with a cooled tip (FORSEA MTC-3 type, China) was devised to better enable microwave ablation of liver cancers. The efficacy of this technology was evaluated.
The records of 160 patients (97 with hepatoma/hepatocellular carcinoma, 63 with metastatic cancer to the liver) who had undergone microwave ablation with this new device were reviewed. One year survival in 86 patients whose follow-up had been more than one year was determined. Preoperative and post-operative contrast-enhanced CT scans were performed to assess completion of therapy and the presence or absence of recurrent tumor. For patients with hepatoma/hepatocellular carcinoma, serum AFP levels were evaluated pre-operatively and, if elevated, post-operatively.
A mean 2.2 (range 1-5) applications were required per session. All patients exhibited initial radiographic resolution of their lesions after therapy. The ablated areas were not enhanced in every phases of contrast CT scan. Eight patients required a second microwave therapy for recurrent tumor; two patients required a third treatment. Twenty five patients with hepatoma/hepatocellular carcinoma had elevated AFP (104.2+/-22.5 ng/ml), which, after microwave ablation, recovered to normal or almost normal (24.6+/-3.6 ng/ml) (t=2.1, p<0.05). There were no post-operative deaths. Complications included fever in three of four patients, successfully treated with indomethacin, elevated transaminases in four of five patients, requiring no treatment except for those with pre-operative ascites (who were dialyzed), pleural effusions in fourteen patients, only one of whom required drainage, and obstructive jaundice requiring drainage in two patients. The one year survival fraction was 91.9%.
Microwave ablation with this novel cooled-tip electrode is safe, minimally invasive and effective. The tool may greatly expand the fraction of patients with liver cancer who might be candidates for microwave ablation.
Hepato-gastroenterology 55(88):2184-7. · 0.66 Impact Factor
