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ABSTRACT: A light-harvesting chlorophyll a/b-protein complex (LHCP) was isolated directly from thylakoid membranes of marine green alga,Bryopsis corticulans, by two consecutive runs of liquid chromatography. The trimeric form of the light-harvesting complex has been obtained by
sucrose gradient ultracentrifugation. The result of SDS-PAGE shows that the light-harvesting complex is composed of at least
five apoproteins in which a protein with apparent molecular weight of about 31 kD was never found in the major light-harvesting
complex (LHC II) from higher plants. The isolatedBryopsis corticulans light-harvesting complex contains a specific carotenoid, siphonaxanthin, as well as chlorophyll (Chl)a, Chlb, neoxanthin and violaxanthin. Siphonaxanthin which is present in the light-harvesting siphonaxanthin-chlorophylla/b-protein complex ofBryopsis corticulans is responsible for enhanced absorption in the blue-green region (530 nm). Efficient energy transfer from both siphonaxanthin
and Chlb to Chla inBryopsis corticulans LHCP, which has similar absorption and fluorescence emission spectra to those of the lutein-chlorophylla/b-protein of higher plants, proved that molecular arrangement of the light-harvesting pigments was highly ordered in theBryopsis corticulans LHCP. The siphonaxanthin-chlorophyll a/b-proteins allow enhanced absorption of blue-green light, the predominant light available
in deep ocean waters or shaded subtidal marine habitats.
Keywordschlorophyll-protein complex-siphonaxanthin-liquid chromatography-
Bryopsis corticulans
Chinese Science Bulletin 04/2012; 49(18):1936-1941. · 1.32 Impact Factor
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ABSTRACT: This presentation introduces the advances in biological nitrogen fixation research abroad, in particular, describes the great
progress and achievements on its research in China as follows: collection of rhizobial resources and establishment of the
largest database ofRhizobium in China, correction and development ofRhizobium taxonomy in international; discovery of a couple ofnif genes, identification and unification of linkage among thenif gene operonsof Klebsiella pneumoniae, finding of regulative mechanism of positive regulationnif gene and its sensitivity to oxygen, temperature; finding of the activity of nodulation genenodD3 product inSinorhizobium meliloti which is not controlled by flavonoid produced from its host alfalfa; finding of the association between expression of genes
coding the products for carbon utilization and nitrogen metabolism and their regulations; chemical synthesis of nodulation
factor ofSinorhizobium meliloti; constructions of engineered nitrogen fixers and utilization in practice based on the research of gene expression and regulation;
chemical simulation of the structure and function of nitrogenase and bringing forward the model of nitrogenase active center
for the first time in international and synthesis of model compounds which were paid attention by colleagues abroad. Finally,
the development of nitrogen fixation research in China in future has been put forward, suggesting that thenif gene regulation and its role in providing crops with nitrogen element, signal transduction and molecular interactions betweenRhizobium and legume, coupling between carbon and nitrogen metabolisms, nitrogen fixation and photosynthesis, and functional genomics
of nitrogen-fixing nodule symbiosis, etc., would be actively worked on.
Keywordsbiological nitrogen fixation-nitrogenase-gene expression-chemical modeling-interaction between microbes and plants-functional genomes
Chinese Science Bulletin 04/2012; 48(10):954-960. · 1.32 Impact Factor
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ABSTRACT: Sinorhizobium fredii RT19, a strain of freeliving bacteria, was subjected to salt shock and its protein expression profiles were analyzed by differential
display proteome approaches. The results of separation by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) showed
that the number of resolved proteins was 481, 465 and 424, corresponding to salt-free control, 5 and 50 min 1 mol/L salt treatment,
respectively. Among the resolved proteins, 82 in total had altered expression in response to salt-shock stress. 26 out of
the 82 proteins were induced and 23 were completely inhibited, while 12 were up-regulated and 21 down-regulated in response
to salt shock. In addition, the appearance of differentially displayed proteins responding to different salt shock periods
is also reported. The identity of the 26 induced proteins was revealed by matrix assisted laser desorption/ionization time-of-flight
mass spectrometry (MALDI-TOF/MS) followed by database searching. Among them, 20 were assigned to proteins with known functions.
Their roles in response to salt shock stress are discussed.
Keywords
Sinorhizobium fredii
-salt shock-two-dimensional electrophoresis-proteome-MALDI-TOF / MS
Chinese Science Bulletin 04/2012; 49(17):1828-1833. · 1.32 Impact Factor
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ABSTRACT: Jatrpha curcas L., a non-model woody plant belonging to Euphorbiaceae family, is a promising economic plant due to the high oil content in seed and high tolerance to drought and salt stress. The embryo and endosperm of J. curcas seed differ in morphology, function and ploidy. To characterize the protein profiles of these two tissues, we have performed proteomic analysis with the dry mature J. curcas seeds. The data showed that the 2-DE profiles of endosperm and embryo were similar to each other. There are 66 differential proteins between the two seed tissues, in which 28 proteins distributed in 9 distinct functional classes, have been identified successfully in endosperm or embryo. The major groups of differential proteins are associated with metabolism (25%) and disease/defence (18%). Our results demonstrated that in the dry mature J. curcas seeds, the proteins involved in oil mobilization, signal transduction, transcription, protein synthesis, and cell cycle which are essential for the seed germination have occurred in endosperm and embryo, reflecting the fact that proteins required for germination are already present in the dry mature seed.
Plant Science 12/2011; 181(6):660-6. · 2.94 Impact Factor
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ABSTRACT: Water status is the main factor affecting rice production. In order to understand rice strategies in response to drought condition in the field, the drought-responsive mechanisms at the physiological and molecular levels were studied in two rice genotypes with contrasting susceptibility to drought stress at reproductive stage. After 20 d of drought treatment, the osmotic potential of leaves reduced 78% and 8% in drought susceptible rice cultivar Zhenshan97B and tolerant rice cultivar IRAT109, respectively. The panicle lengths had no obvious changes in drought stressed Zhenshan97B and IRAT109, suggesting that drought stress impose less effect on assimilate translocation from leaf to vegetative growth of panicles. IRAT109 showed more extensive deeper root growth that could be considered a second line of defense against drought stress. The C(i)/C(a) ratio exhibited enhancement over reduction of g(s) in both cultivars, reflecting the non-stomatal limitation to photosynthesis occurred during drought stress. Orthophosphate dikinase, glycine dehydrogenase, ribulose bisphosphate carboxylase (Rubisco), glycine hydroxymethyltransferase and ATP synthase were down-regulated for Zhenshan97B in response to drought stress, suggesting the reduction of capacity of carbon assimilation in this rice cultivar. In drought-stressed IRAT109, transketolase, Rubisco were down-regulated, however, Rubisco activase and peptidyl-prolyl cis-trans isomerase, which might alleviate the damage on Rubisco by drought stress, were up-regulated. The increased abundances of chloroplastic superoxide dismutase [Cu-Zn] and dehydroascorbate reductase might provide antioxidant protection for IRAT109 against damage by dehydration.
Journal of plant physiology 12/2011; 169(4):336-44. · 2.50 Impact Factor
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ABSTRACT: Jatropha curcas L. is an all-purpose biodiesel plant and is widely distributed in tropical and subtropical climates. It can grow well on poor quality soil which is not qualified for crop cultivation. This is very important for relieving land, food and energy crises. However, tropical and subtropical distribution limits the production of J. curcas seed. So it is valuable to know the molecular mechanism of J. curcas response to adverse abiotic environmental factors, especially freezing stress, in order to change the plant's characteristics. Until now there are just a few reports about J. curcas molecular biology. In this paper, we cloned and characterized a DNA binding protein from this plant, designated as JcDREB. Sequence analysis and yeast one-hybrid assays show that JcDREB can effectively function as a transcription factor of DREB protein family belonging to A-6 subgroup member. Expression patterns of JcDREB showed that it was induced by cold, salt and drought stresses, not by ABA. Over-expression of JcDREB in transgenic Arabidopsis exhibited enhanced salt and freezing stresses. Understanding the molecular mechanisms of J. curcas responses to environmental stresses, for example, high salinity, drought and low temperature, is crucial for improving their stress tolerance and productivity. This work provides more information about A-6 subgroup members of DREB subfamily.
Plant Science 12/2011; 181(6):623-31. · 2.94 Impact Factor
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ABSTRACT: Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1-DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.
Proteomics 07/2011; 11(13):2693-713. · 4.43 Impact Factor
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ABSTRACT: Grazing is accompanied by a multitude of processes including wounding, saliva deposition, and defoliation. Previous studies have focused on the effects of the grazing or clipping intensity on plant regrowth, survival, and composition in the grassland. However, the impact of saliva deposition on plants is poorly understood. In this study, rice was used as a model plant to study the differentially expressed proteins after ovine saliva treatment. The shoots of 2-week-old seedlings were crosscut and the lower parts were daubed with ovine saliva at the cut surface. After 2, 6, 12 and 24h, proteomics analysis was performed using proteins extracted from the saliva-treated shoots. The results showed that proteins involved in multiple pathways were differentially expressed in response to ovine saliva, including catalase (CAT), peroxiredoxin (Prx), ATP synthase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Moreover, real-time quantitative reverse-transcription-PCR (RT-PCR) data showed that most of the genes were also regulated at the transcript level. Our results indicate the ovine saliva induces an early response in the rice seedling by stress-related pathways. This study provides information about the response of rice seedlings to ovine saliva at the protein level.
Journal of plant physiology 10/2010; 168(5):500-9. · 2.50 Impact Factor
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ABSTRACT: Rhizobial endophytes infect and colonize not only leguminous plants, but several non-leguminous species as well. Using green fluorescent protein tagging technique, it has been shown that Rhizobia infect different varieties of rice species and migrate from plant roots to aerial tissues such as leaf sheaths and leaves. The interaction between them was found to promote the growth of rice. The growth promotion is the cumulative result of enhanced photosynthesis and stress resistance. In addition, indole-3-acetic acid also contributes to the promotion. Gel-based comparative proteomic approaches were applied to analyze the protein profiles of three different tissues (root, leaf sheath and leaf) of Sinorhizobium meliloti 1021 inoculated rice in order to get an understanding about the molecular mechanism. Upon the inoculation of rhizobia, proteins involved in nine different functional categories were either up-regulated or down-regulated. Photosynthesis related proteins were up-regulated only in leaf sheath and leaf, while the up-regulated proteins in root were exclusively defense related. The results implied that there might have been an increase in the import and transport of proteins involved in light and dark reactions to the chloroplast as well as more efficient distribution of nutrients, hence enhanced photosynthesis. Although the initiation of defensive reactions mainly occurred in roots, some different defense mechanisms were also evoked in the aerial tissues.
Proteomics 03/2010; 10(9):1861-74. · 4.43 Impact Factor
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ABSTRACT: China is rich in energy plant resources. In this article, 64 plant species are identified as potential energy plants in China. The energy plant species include 38 oilseed crops, 5 starch-producing crops, 3 sugar-producing crops and 18 species for lignocellulosic biomass. The species were evaluated on the basis of their production capacity and their resistance to salt, drought, and/or low temperature stress. Ten plant species have high production and/or stress resistance and can be potentially developed as the candidate energy plants. Of these, four species could be the primary energy plants in China: Barbados nut (Jatropha curcas L.), Jerusalem artichoke (Helianthus tuberosus L.), sweet sorghum (Sorghum bicolor L.) and Chinese silvergrass (Miscanthus sinensis Anderss.). We discuss the use of biotechnological techniques such as genome sequencing, molecular markers, and genetic transformation to improve energy plants. These techniques are being used to develop new cultivars and to analyze and manipulate genetic variation to improve attributes of energy plants in China.
Environmental Management 02/2010; 46(4):579-89. · 1.74 Impact Factor
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ABSTRACT: Cold stress has adverse effects on plant growth and development. Plants respond and acclimate to cold stress through various biochemical and physiological processes, thereby acquiring stress tolerance. To better understand the basis for tolerance, we carried out a proteomic study in the model moss, Physcomitrella patens, characterizing gametophore proteins with 2-DE and mass spectroscopy. Following exposure to 0 degrees C for up to 3 days, out of the more than 1000 protein spots reproducibly resolved, only 45 changed in abundance by at least 1.5-fold. Of these, 35 were identified by tryptic digestion and mass spectroscopy. Photosynthetic proteins decreased, whereas many catabolic proteins increased. In addition, cold stress up-regulated a variety of signaling, cytoskeleton, and defense proteins and few proteins in these classes were down-regulated. Up-regulated proteins include the 14-3-3-like protein, actin, HSP70s, lipoxygenases, and cytochrome P450 proteins. These results point to pathways that are important for the mechanism of cold stress response in P. patens and by extension to the entire plant kingdom.
Proteomics 09/2009; 9(19):4529-38. · 4.43 Impact Factor
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ABSTRACT: Physcomitrella patens is well known because of its importance in the study of plant systematics and evolution. The tolerance of P. patens for high-salinity environments also makes it an ideal candidate for studying the molecular mechanisms by which plants respond to salinity stresses. We measured changes in the proteome of P. patens gametophores that were exposed to high-salinity (250, 300, and 350 mM NaCl) using two-dimensional gel electrophoresis (2-DE) via liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sixty-five protein spots were significantly altered by exposure to the high-salinity environment. Among them, 16 protein spots were down-regulated and 49 protein spots were up-regulated. These proteins were associated with a variety of functions, including energy and material metabolism, protein synthesis and degradation, cell defense, cell growth/division, transport, signal transduction, and transposons. Specifically, the up-regulated proteins were primarily involved in defense, protein folding, and ionic homeostasis. In summary, we outline several novel insights into the response of P. patens to high-salinity; (1) HSP70 is likely to play a significant role in protecting proteins from denaturation and degradation during salinity stress, (2) signaling proteins, such as 14-3-3 and phototropin, may work cooperatively to regulate plasma membrane H(+)-ATPase and maintain ion homeostasis, (3) an increase in photosynthetic activity may contribute to salinity tolerance, and (4) ROS scavengers were up-regulated suggesting that the antioxidative system may play a crucial role in protecting cells from oxidative damage following exposure to salinity stress in P. patens.
Planta 07/2008; 228(1):167-77. · 3.00 Impact Factor
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ABSTRACT: Although seed germination is a major subject in plant physiological research, there is still a long way to go to elucidate the mechanism of seed germination. Recently, functional genomic strategies have been applied to study the germination of plant seeds. Here, we conducted a proteomic analysis of seed germination in rice (Oryza sativa indica cv. 9311) - a model monocot. Comparison of 2-DE maps showed that there were 148 proteins displayed differently in the germination process of rice seeds. Among the changed proteins, 63 were down-regulated, 69 were up-regulated (including 20 induced proteins). The down-regulated proteins were mainly storage proteins, such as globulin and glutelin, and proteins associated with seed maturation, such as "early embryogenesis protein" and "late embryogenesis abundant protein", and proteins related to desiccation, such as "abscisic acid-induced protein" and "cold-regulated protein". The degradation of storage proteins mainly happened at the late stage of germination phase II (48 h imbibition), while that of seed maturation and desiccation associated proteins occurred at the early stage of phase II (24 h imbibition). In addition to alpha-amylase, the up-regulated proteins were mainly those involved in glycolysis such as UDP-glucose dehydrogenase, fructokinase, phosphoglucomutase, and pyruvate decarboxylase. The results reflected the possible biochemical and physiological processes of germination of rice seeds.
PROTEOMICS 10/2007; 7(18):3358-68. · 4.51 Impact Factor
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ABSTRACT: Soil salinization has become a severe global problem and salinity is one of the most severe abiotic stresses inhibiting growth and survival of mycorrhizal fungi and their host plants. Salinity tolerance of ectomycorrhizal fungi and survival of ectomycorrhizal inocula is essential to reforestation and ecosystem restoration in saline areas. Proteomic changes of an ectomycorrhizal fungus, Boletus edulis, when exposed to salt stress conditions (4% NaCl, w/v) were determined using two-dimensional electrophoresis (2DE) and mass spectrometry (MS) techniques. Twenty-two protein spots, 14 upregulated and 8 downregulated, were found changed under salt stress conditions. Sixteen changed protein spots were identified by nanospray ESI Q-TOF MS/MS and liquid chromatography MS/MS. These proteins were involved in biosynthesis of methionine and S-adenosylmethionine, glycolysis, DNA repair, cell cycle control, and general stress tolerance, and their possible functions in salinity adaptation of Boletus edulis were discussed.
Mycological Research 09/2007; 111(Pt 8):939-46. · 2.81 Impact Factor
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ABSTRACT: Two-week-old dark-grown rice seedlings were de-etiolated upon exposure to light. A comparison of 2-DE protein profiles between the dark-grown control and the rice seedlings illuminated respectively for 6, 12 and 24 h revealed 52 differentially expressed CBB-stained spots. Of these changed spots, the identity of 51 protein spots was determined by MALDI-TOF MS. Of these identified proteins, 13 proteins were related to light reactions of photosynthesis, photosynthetic carbon assimilation and chlorophyll biosynthesis, indicating the complex process of biogenesis of photosynthetic apparatus was correlated to the transition from a dark-grown (etiolated) to a light-grown (de-etiolated) morphology. In addition, three proteins related to antioxidation and detoxification decreased in de-etiolated rice seedlings implied, that the etiolated rice seedlings possibly be under an oxidative stress which could be released during their early stages of de-etiolation. The increase of S-adenosylmethionine synthetase that is involved in the biosynthesis of the phytohormone ethylene might contribute to the phenotypic development of the apical hook in the de-etiolated rice seedlings. These results yield a comprehensive picture of the post-transcriptional response for de-etiolation of rice seedlings and serve as a basic platform for further characterization of gene function and regulation in light-induced development of plants.
PROTEOMICS 08/2007; 7(14):2459-68. · 4.51 Impact Factor
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ABSTRACT: A cDNA clone, named JcERF, was isolated from Jatropha curcas seedlings (a woody oil plant). It was classified as an ERF subfamily member based on multiple sequence alignment and phylogenetic characterization. The deduced amino acid sequences of the JcERF clone showed no significant sequence similarity with other known ERF proteins except for the conserved AP2/EREBP DNA-binding domain. Expression of the JcERF gene was rapidly induced upon salinity, drought, ethylene and mechanical wounding treatments. No significant changes in the JcERF expression were observed under ABA stress. Gel retardation assay revealed that the JcERF protein could bind specifically to the GCC box as well as to the C/DRE motif. Also it can be inferred from the gel-shift that there is a possibility that the near sequence of the GCC box has an important effect on the DNA-binding activity. In yeast, the JcERF protein specifically bound to the DRE sequence and activated the transcription of two reporter genes His3 and LacZ driven by the DRE sequence. When fused to the LexA DNA-binding domain, the full-length JcERF functioned effectively as a trans-activator in the yeast one-hybrid assay. Overexpression of JcERF cDNA in transgenic Arabidopsis enhanced the salt and freezing tolerance. Meanwhile the seed germination of JcERF transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that JcERF functioned as a novel transcription factor and it exhibited a mechanism of plant response to environmental factors like the other AP2/EREBP regulons that also exist in tropical woody plants.
Plant Molecular Biology 03/2007; 63(3):419-28. · 4.15 Impact Factor
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ABSTRACT: To investigate roles of the actin cytoskeleton in growth of the pollen tube of Picea meyeri, we used the actin polymerization inhibitor latrunculin B (LATB) under quantitatively controlled conditions. At low concentrations, LATB inhibited polymerization of the actin cytoskeleton in the growing pollen tube, which rapidly inhibited tip growth. The proteomic approach was used to analyse protein expression-profile changes during pollen germination and subsequent pollen-tube development with disturbed organization of the actin cytoskeleton. Two-dimensional electrophoresis and staining with Coomassie Brilliant Blue revealed nearly 600 protein spots. A total of 84 of these were differentially displayed at different hours with varying doses of LATB, and 53 upregulated or downregulated proteins were identified by mass spectrometry. These proteins were grouped into distinct functional categories including signalling, actin cytoskeleton organization, cell expansion and carbohydrate metabolism. Moreover, actin disruption affected the morphology of Golgi stacks, mitochondria and amyloplasts, along with a differential expression of proteins involved in their functions. These findings provide new insights into the multifaceted mechanism of actin cytoskeleton functions and its interaction with signalling, cell-expansion machinery and energy-providing pathways.
The Plant Journal 08/2006; 47(2):174-95. · 6.16 Impact Factor
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ABSTRACT: Uppermost internodes, which connect the part between the ear and lower stem, form an important pathway transporting mineral nutrition from roots and photosynthates from leaves (especially the flag leaf) to the ear. The milky stage is the first stage of seed ripening. The uppermost internodes of rice at the milky stage are critical for seed quality and yield. Total soluble proteins of the uppermost internodes of rice (Oryza sativa L. ssp. indica) at the milky stage were analyzed using proteomic methods. Using 2-DE, 762 reproducible protein spots were detected. Among them, 132 abundant proteins were analyzed using MALDI-TOF-MS. Searching in the National Center for Biotechnology Information database, we could identify 98 proteins, which represent 80 gene products. These proteins belong to 11 functional groups with energy production-associated proteins in the first place. The large accumulation of proteins involved in metabolism, signaling, and stress resistance indicated that the uppermost internodes of rice have a high physiological and stress-resistant activity. In addition, our results will also enrich the database of the rice proteome.
PROTEOMICS 07/2006; 6(11):3330-8. · 4.51 Impact Factor
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ABSTRACT: Liangyoupeijiu is a two-parental-line, and Shanyou63 is a three-parental-line hybrid rice (Oryza sativa L.). Although both belong to the indica subspecies, they have obvious differences with respect to morphology, physiology and grain quality. Variations in endosperm protein compositions were studied by comparing the 2-D electrophoresis (2-DE) maps for these two cultivars of hybrid rice. After matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) analysis, a 21-kDa precursor of 19-kDa globulin was identified as the major storage protein for both cultivars. Some isoforms of peroxiredoxin and seed maturation protein were found to only exist in Shanyou63, whereas aldose reductase and starch granule-bound starch synthase were only detected in Liangyoupeijiu. These data might provide a founda-tion for further comparative studies of these two cultivars of hybrid rice.
Journal of Integrative Plant Biology 01/2006; 48:1028-1033. · 2.53 Impact Factor
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ABSTRACT: A new DRE-binding protein gene FaDREB1 encoded for an AP2/ERFBP-type transcription factor was isolated by RACE-PCR from Festuca arundinacea Schreb seedlings. Its cDNA was sequenced with 988 bp, from which a protein with 216 amino acid residues was deduced with a predicted molecular mass of 23.479 kDa and a pI of 4.70. A search of the Protein Blast data revealed that this protein can be classified as a typical member of the AP2/EREBP family of DNA-binding proteins. The tissue organ-specific expression pattern of the FaDREB1 gene showed that its transcripts were abundant in leaves and leaf sheaths, and scarce in roots. Southern blot analysis indicated that it is a multiple-copy gene. Its mRNA accumulation profiles made clear that its expression was strongly induced by cold treatment, weakly induced by drought and salt stress, but did not respond to ABA treatment. It was concluded that the protein FaDREB1 may be involved in the process of plant response to cold stress through an ABA-independent pathway.
Plant Physiology and Biochemistry 04/2005; 43(3):233-9. · 2.84 Impact Factor
