B. Puerto

University of Barcelona, Barcino, Catalonia, Spain

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Publications (179)581.4 Total impact

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    ABSTRACT: Study question: Are the levels of circulating cell-derived microparticles (cMPs) in patients with recurrent miscarriage (RM) associated with the antiphospholipid syndrome (APS)? SUMMARY ANSWER: cMPs in women with RM are not associated with antiphospholipid antibodies (aPLs). What is known already: Previous studies have focused on cMP levels in RM patients. Most studies have shown higher levels of cMPs in RM patients whereas others have reported lower levels. Data regarding cMPs in patients with the APS are scanty in the literature. Study design, size, duration: A case-control study including three groups of patients. A total of 154 women were prospectively recruited from September 2009 to October 2013. Four patients refused to participate. The APS group consisted of 50 women that had been previously diagnosed with primary APS and had had ≥3 consecutive first trimester miscarriages. The uRM group included 52 couples with ≥3 consecutive first trimester miscarriages of unknown etiology. The fertile control (FER) group was composed of 52 healthy fertile women with no history of pregnancy losses. Miscarriage was defined as intrauterine pregnancy loss at <10 weeks' size on ultrasound. Participants/materials, setting, methods: Venous blood samples for coagulation studies and cMP determinations were obtained. All patients underwent a thrombophilia study. MAIN RESULTS AND THE ROLE OF CHANCE: cMP levels were significantly higher in the APS and uRM groups versus the FER group (P < 0.0001 and P = 0.009, respectively) (cMP number × 10(3)/ml plasma [mean ± SD]: APS: 18.5 ± 13.6; uRM: 16.3 ± 13.8; FER: 9.7 ± 4.6). There were no statistically significant differences in cMP levels between the APS and uRM groups. Limitations, reasons for caution: The sample size was arbitrarily decided according to previous studies analyzing cMPs in RM patients. Different cMP subtypes were not investigated. Wider implications of the findings: The present study adds further data on the subject showing that patients with RM, irrespective of testing positive for aPLs, have increased levels of cMPs compared with healthy fertile controls. The presence of elevated cMPs in RM women may reflect an ongoing systemic pathological, albeit asymptomatic, status that can become deleterious in the setting of pregnancy. Study funding/competing interests: This study was supported in part by grant from FIS-PI11/01560 within the 'Plan Nacional de I+D+I' and co-funded by the 'ISCIII-Subdirección General de Evaluación' and the 'Fondo Europeo de Desarrollo Regional (FEDER)'. The authors have no competing interests to disclose. Trial registration number: Not applicable.
    Human Reproduction 11/2015; DOI:10.1093/humrep/dev278 · 4.57 Impact Factor
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    ABSTRACT: Objective: To explore corpus callosum (CC) developmental differences by ultrasound in late-onset small fetuses compared with adequate for gestational age (AGA) controls. Study design: Ninety four small (estimated fetal weight <10th centile) and 71 AGA fetuses were included. Small fetuses were further subdivided into fetal growth restriction (IUGR, n = 64) and small for gestational age (SGA, n = 30) based on poor perinatal outcome factors, that is, birth weight <3rd centile and/or abnormal cerebroplacental ratio and/or uterine artery Doppler. The entire cohort was scanned to assess CC by transvaginal neurosonography obtaining axial, coronal and midsagittal images. CC length, thickness, total area and the areas after a subdivision in 7 portions were evaluated by semiautomatic software. Furthermore, the weekly average growth of the CC in each study group was calculated and compared. Results: Small fetuses showed significantly shorter (small fetuses: 0.49 vs. AGA: 0.52; p < 0.01) and smaller CC (1.83 vs. 2.03; p < 0.01) with smaller splenium (0.47 vs. 0.55; p < 0.01) compared to controls. The CC growth rate was also reduced when compared to controls. Changes were more prominent in small fetuses with abnormal cerebroplacental Doppler suggesting fetal growth restriction. Conclusions: Neurosonographic assessment of CC showed significantly altered callosal development, suggesting in-utero brain reorganization in small fetuses. This data support the potential value of CC assessment by US to monitor brain development in fetuses at risk.
    Fetal Diagnosis and Therapy 02/2015; 37(4). DOI:10.1159/000366160 · 2.94 Impact Factor

  • Ultrasound in Obstetrics and Gynecology 09/2014; 44(S1):114-114. DOI:10.1002/uog.13844 · 3.85 Impact Factor

  • Ultrasound in Obstetrics and Gynecology 09/2014; 44(S1):100-100. DOI:10.1002/uog.13758 · 3.85 Impact Factor
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    ABSTRACT: to determine the prevalence and sonographic appearance of the hippocampal commissure in fetuses with isolated complete agenesis of the corpus callosum by three-dimensional (3D) neurosonography in the multiplanar mode. a multicenter observational study. Stored volume datasets of fetuses with isolated complete agenesis of the corpus were retrospectively retrieved for analysis in three tertiary centres. The presence or absence of the hippocampal commissure was independently evaluated in the coronal and in the midsagittal planes by two operators. Postnatal follow-up was obtained in all cases. from November 2007 to February 2013, 41 cases between 19 and 30 weeks of gestation were retrieved for analysis. The hippocampal commissure was visible in the coronal and in the sagittal planes in 27/41 (65.8%), absent or not clearly recognizable in the remaining 14 cases. The qualitative analysis of the two operators was concordant in 100% of cases. in more than half of fetuses with complete callosal agenesis the hippocampal commissure may be visualized at antenatal ultrasound. This is a residual inter-hemispheric connection, which in normal cases is hidden by the corpus callosum itself. Further research is needed to establish if this has an impact on postnatal outcome. This article is protected by copyright. All rights reserved.
    Ultrasound in Obstetrics and Gynecology 09/2014; 44(S1):36-37. DOI:10.1002/uog.13561 · 3.85 Impact Factor

  • Ultrasound in Obstetrics and Gynecology 09/2014; 44(S1):200-200. DOI:10.1002/uog.14049 · 3.85 Impact Factor

  • Ultrasound in Obstetrics and Gynecology 09/2014; 44(S1):231-231. DOI:10.1002/uog.14153 · 3.85 Impact Factor

  • Ultrasound in Obstetrics and Gynecology 09/2014; 44(S1):36-36. DOI:10.1002/uog.13560 · 3.85 Impact Factor
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    ABSTRACT: Objectives The aim of our study was to evaluate the association of CHD on head biometry and cerebrovascular blood flow dynamics at the time of diagnosis in the second trimester of pregnancy.Material and Methods95 consecutive fetuses diagnosed with CHD. At the time of diagnosis of CHD, we assessed fetal biometry and brain perfusion by middle cerebral artery pulsatility index (MCA-PI), cerebroplacental ratio (CPR) and Fractional Moving Blood Volume (FMBV). The results were compared with 95 normal fetuses matched for gestational age (GA).ResultsMedian gestational age at diagnosis was 22.3 weeks (range 20 – 23.5 weeks). Fetuses with CHD showed significantly lower MCA-PI and CPR Z-scores (−0.23 vs 0.34 and −0.37 vs 0.30; p < 0.001, respectively) and higher FMBV Z-scores (2.35 vs 0.15; p < 0.001). FMBV >95th centile was observed in 81.1% of cases as compared with 10.5% in controls (p < 0.001). Moreover, cases showed significantly smaller biparietal diameter (BPD) and head circumference (HC) Z-scores (−1.61 vs −0.43 and −0.89 vs 0.09; p < 0.001, respectively) and a higher proportion of BPD and HC measurements below the 5th percentile compared with controls (51.6% vs 13.7% and 26.3% vs 4.2%; p < 0.001, respectively).These findings were more pronounced in those CHD with compromised oxygenated blood delivery to the brain, such as left outflow tract obstruction and transposition of the great arteries.ConclusionsA high proportion of fetuses with CHD have smaller head and increased brain perfusion already in the second trimester, suggesting an early onset of the mechanisms leading to poorer neurodevelopment later in life.
    Ultrasound in Obstetrics and Gynecology 08/2014; 44(2). DOI:10.1002/uog.13373 · 3.85 Impact Factor
  • Sofia Hakim · Magda Sanz-Cortes · Bienvenido Puerto · Teresa Ribalta · Alfons Nadal ·
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    ABSTRACT: Abstract We describe the first case of meningioangiomatosis identified in a second trimester fetus. A 30-year old pregnant woman was attended at our hospital for a second-trimester ultrasound control. With a diagnosis of partial agenesis of the corpus callosum, parents request the termination of pregnancy. At autopsy, frontal serial sections of the fetal brain disclosed a short corpus callosum that lacked the posterior splenium, confirming the ultrasound diagnosis. At close inspection, a slight bilateral hardening of both medial aspects of frontal lobes and anterior genu of corpus callosum was found associated with meningeal adhesion between both frontal lobes. Microscopically, cerebral cortex and corpus callosum were permeated by intersecting bundles of spindle cells with eosinophilic cytoplasm and bland, round nuclei, with a fibroblast or meningothelial-like appearance surrounding abundant blood vessels consistent with the diagnosis of meningioangiomatosis. According to this finding, meningioangiomatosis must be included in the differential diagnosis of meningo-cortical fetal lesions.
    Pediatric and Developmental Pathology 03/2014; 17(4). DOI:10.2350/13-08-1366-CR.1 · 0.87 Impact Factor
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    ABSTRACT: OBJECTIVES: To evaluate in a cohort of 248 fetuses seen at a tertiary referral center, the frequency of isolated ventricular septal defects (VSD) among all congenital heart defects (CHD), the association with chromosomal and post-natal anomalies, and the rate of spontaneous closure. PATIENTS AND METHODS: A 6-year study on 10800 patients referred for a cardiac scan, with 995 confirmed congenital heart diseases (CHD). The prevalence and characteristics of VSDs were analyzed, including follow-up until one year of age. A multivariate binary logistic regression was performed to test the independent contribution of the VSD/Aorta ratio (<0.5 and ≥0.5) and location of VSD (perimembranous and muscular) in the prediction of spontaneous closure before one year of life. RESULTS: 248 (24.9% of all CHD) VSDs were diagnosed, 216 (87.1%) muscular and 32 (12.9%) perimembranous. Median GA at diagnosis was 30.4 (range 17-41) weeks and mean size 2.6 (SD, 0.77) mm. Serious chromosomal anomalies were found in 1 (3.1%) perimembranous VSD compared to none of 216 muscular defect (p=0.12). Post-natal malformations were diagnosed in 8 cases (3.8%). Spontaneous closure occurred in 13 fetuses (5.32%) and 151 infants (76.3%), and it was predicted by VSD/Aorta ratio (OR 0.445; 95% CI 0.216-0.914, p<0.03) and location (OR 0.385, 95% CI 0.160-0.926, p<0.0.03). CONCLUSIONS: In a fetal cardiology unit, isolated muscular VSD is today the most prevalent CHD. Contrary to post-natal series muscular VSD were the most common. Perimembranous VSD was associated with a higher risk of chromosomal anomalies. Muscular VSD had similar risks to normal pregnancies. Spontaneous closure was frequent and occurred in most cases postnatally.
    Ultrasound in Obstetrics and Gynecology 01/2014; 43(1). DOI:10.1002/uog.12527 · 3.85 Impact Factor
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    ABSTRACT: Objective: Congenital high airway obstruction syndrome (CHAOS) normally has a dire prognosis due to laryngeal atresia and association with other anomalies. However, rare cases with an isolated tracheal membrane have been described. We assessed systematically the larynx and vocal cords in fetuses with CHAOS, with the goal of identifying such cases and attempting fetoscopic deobstruction. Methods: Between 2008 and 2012, 7 cases of CHAOS were referred to our institution. The ultrasonographic aspect of the larynx and trachea was assessed. We report the ultrasound findings, necropsy findings of 6 cases and perinatal outcome in 1 case undergoing therapy. Results: In 6 cases, laryngeal atresia was presumed by ultrasound and confirmed by necropsy after termination of pregnancy. In 1 case, normal appearance of the vocal cords led to suspicion of a possible isolated tracheal web completely occluding the trachea. Fetoscopy confirmed the finding, thus the membrane was perforated. Lung size normalized and hydrops resolved. A normal fetus was delivered at term, requiring no respiratory support. At 9 months of age, the infant showed a normal laryngoscopy and was developing well. Conclusions: Systematic ultrasound assessment of the vocal cords can be performed in fetuses with CHAOS. We report the first case of long-term intact survival after fetoscopic airway deobstruction.
    Fetal Diagnosis and Therapy 07/2013; 34(2). DOI:10.1159/000350697 · 2.94 Impact Factor
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    ABSTRACT: To construct reference ranges for cisterna magna (CM) width at 11–13 weeks' gestation in healthy fetuses and determine whether open spina bifida and posterior fossa anomalies could be diagnosed in the first trimester. This was a retrospective study. CM width reference ranges were constructed based on the measurements obtained from 80 healthy fetuses with normal postnatal outcome undergoing routine first-trimester ultrasound at 11–13 weeks, using the Lambda-Mu-Sigma method. CM was measured in the fetal mid-sagittal view, as routinely used for nuchal translucency assessment. In addition, first-trimester ultrasound images in 11 fetuses with open spina bifida or posterior fossa anomalies, most of which were diagnosed later in pregnancy, were retrospectively reviewed, and CM measurements were compared against reference ranges. CM width was noted to increase with gestational age in normal fetuses. The anomalies in the 11 fetuses we reviewed were: open spina bifida (n = 5), megacisterna magna (n = 3), Blake's pouch cyst (n = 2) and posterior fossa arachnoid cyst (n = 1). All fetuses with open spina bifida had a CM width below the 5th percentile. In the two fetuses with Blake's pouch cyst and in two with megacisterna magna, CM width was above the 95th percentile. In one of the fetuses with a megacisterna magna and the one with an arachnoid cyst, CM width was within normal range. We have constructed reference ranges for CM width at 11–13 weeks using the mid-sagittal view. It appears that first-trimester CM width can be used as a marker for the early detection of open spina bifida. However, our findings need to be confirmed in prospective large series. Copyright © 2012 ISUOG. Published by John Wiley & Sons, Ltd.
    Ultrasound in Obstetrics and Gynecology 05/2013; 41(5). DOI:10.1002/uog.12302 · 3.85 Impact Factor
  • Robin Julve · Ana Muñoz · Bienvenido Puerto · Magdalena Sanz ·

    Diagnostico Prenatal 03/2013; 24(1):38–41. DOI:10.1016/j.diapre.2012.01.005
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    ABSTRACT: Objective: The objective of this prospective study was to determine the feasibility, safety and performance of a new method for sentinel lymph node (SLN) detection in endometrial cancer (EC) using transvaginal ultrasound-guided myometrial injection of radiotracer (TUMIR). Methods: From 2006 to 2011, 74 patients with high-risk EC were included in the study. Twenty-four hours before surgery 148MBq of (99m)Tc-nanocolloid (8mL) was injected into two spots in the anterior and posterior myometrium using an ultrasound-guided transvaginal puncture. SLN was localized preoperatively by lymphoscintigraphy and intraoperatively with gamma probe. After SLN biopsy the patients underwent a complete laparoscopic pelvic and paraaortic lymphadenectomy. Results: The TUMIR method was successfully achieved in 67/74 patients (90.5%). SLN was identified in 55 women (74.3%). No adverse effects were observed. Pelvic drainage was observed in 87.2% of women and paraaortic SLN was identified in 45.4%, with 12.8% of the patients draining only in this area. The mean number of SLN retrieved was 2.8 per patient (range 1 to 9). Metastatic disease was found in 13 (23.6%) patients. Metastatic involvement of the paraaortic lymph nodes was observed in 4 (30.7%) cases. All were identified by TUMIR. The sensitivity and negative predictive value of SLN detected by TUMIR to detect metastasis were 92.3% (95% CI 22.9-100) and 97.7% (95% CI 82.0-100), respectively. Conclusions: TUMIR is a safe, feasible method to detect SLN in patients with EC, has a good detection rate and provides representative information of the lymphatic drainage of EC.
    Gynecologic Oncology 10/2012; 128(1). DOI:10.1016/j.ygyno.2012.10.008 · 3.77 Impact Factor

  • Ultrasound in Obstetrics and Gynecology 09/2012; 40(S1):16-16. DOI:10.1002/uog.11273 · 3.85 Impact Factor
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    Ultrasound in Obstetrics and Gynecology 09/2012; 40(S1). DOI:10.1002/uog.11369 · 3.85 Impact Factor
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    Ultrasound in Obstetrics and Gynecology 09/2012; 40(S1). DOI:10.1002/uog.11507 · 3.85 Impact Factor
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    Ultrasound in Obstetrics and Gynecology 09/2012; 40(S1). DOI:10.1002/uog.11533 · 3.85 Impact Factor
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    ABSTRACT: This is the first report of fetoscopy to successfully treat a case of bilobar bronchial atresia presenting in the form of a massive right lung tumor causing severe thoracic compression with mediastinal shift, pulmonary hypoplasia and eventually fetal hydrops. The presence of an echolucent tubular structure in the pulmonary hilum was identified as the intrapulmonary continuation of the interrupted main bronchus, and led to the diagnosis of main bronchial atresia. After extensive discussion and counseling, a fetoscopic operation was designed and indicated. The procedure consisted of a fetal tracheoscopy and access to the right main bronchus, which was perforated with a diode laser until a communication with the dead end of the intrapulmonary main bronchus was achieved. After the operation a significant reduction in size was observed, hydrops and mediastinal shift disappeared and O/E LHR increased to 85%. The fetus was born at term and lobectomy of two dysplastic pulmonary lobes was performed. The infant is developing well at 14 months of age. This report demonstrates that fetoscopic airway decompression can achieve fetal survival in the rare event of main bronchus atresia.
    Fetal Diagnosis and Therapy 07/2012; 33(1). DOI:10.1159/000339681 · 2.94 Impact Factor

Publication Stats

2k Citations
581.40 Total Impact Points


  • 1990-2015
    • University of Barcelona
      • Department of Obstetrics and Gynecology, Pediatrics, Radiology and Anatomy
      Barcino, Catalonia, Spain
  • 1991-2014
    • Hospital Clínic de Barcelona
      • • Servicio de Medicina Materno Fetal
      • • Servicio de Enfermedades Autoinmunes y Sistémicas
      Barcino, Catalonia, Spain
  • 2003-2013
    • IDIBAPS August Pi i Sunyer Biomedical Research Institute
      Barcino, Catalonia, Spain
  • 2012
    • Centro de Investigación Biomédica en Red de Enfermedades Raras
      Valenza, Valencia, Spain
  • 2005-2007
    • Southern Medical Clinic
      San Fernando, City of San Fernando, Trinidad and Tobago
  • 2004
    • Institut Marqués, Spain, Barcelona
      Barcino, Catalonia, Spain