Marianne Matzke

Publications of Marianne Matzke

• Hydrolysis of sulphonamides in aqueous solutions.

  Authors: Anna Białk-Bielińska, Stefan Stolte, Marianne Matzke, Aleksandra Fabiańska, Joanna Maszkowska, Marta Kołodziejska, Beata Liberek, Piotr Stepnowski, Jolanta Kumirska

  Journal of hazardous materials. 04/2012;

  Hydrolysis is one of the most common reactions controlling abiotic degradation and is one of the main paths by which substances are degraded in the environment. Nevertheless, the availableHydrolysis is one of the most common reactions controlling abiotic degradation and is one of the main paths by which substances are degraded in the environment. Nevertheless, the available information on this process for many compounds, including sulphonamides (a group of antibiotic drugs widely used in veterinary medicine), is very limited. This is the first study investigating the hydrolytic stabilities of 12 sulphonamides, which were determined according to OECD guideline 111 (1st category reliability data on the basis of regulatory demands on data quality for the environmental risk assessment of pharmaceuticals). Hydrolysis behaviour was examined at pH values normally found in the environment. This was prefaced by a discussion of the acid-base properties of sulphonamides. All the sulphonamides tested were hydrolytically stable at pH 9.0, nine (apart from sulphadiazine, sulphachloropyridazine and sulphamethoxypyridazine) were stable in this respect at pH 7.0 and two (sulphadiazine and sulphaguanidine) at pH 4.0 (hydrolysis rate≤10%; t(0.5 (25°C))>1 year). The degradation products were identified, indicating two independent mechanisms of this process. Our results show that under typical environmental conditions (pH and temperature) sulphonamides are hydrolytically stable with a long half-life; they thus contribute to the on-going assessment of their environmental fate.

• Ecotoxicity evaluation of selected sulfonamides.

  Authors: Anna Białk-Bielińska, Stefan Stolte, Jürgen Arning, Ute Uebers, Andrea Böschen, Piotr Stepnowski, Marianne Matzke

  Chemosphere. 07/2011; 85(6):928-33.

  Sulfonamides (SAs) are a group of antibiotic drugs widely used in veterinary medicine. The contamination of the environment by these pharmaceuticals has raised concern in recent years. However,Sulfonamides (SAs) are a group of antibiotic drugs widely used in veterinary medicine. The contamination of the environment by these pharmaceuticals has raised concern in recent years. However, knowledge of their (eco)toxicity is still very basic and is restricted to just a few of these substances. Even though their toxicological analysis has been thoroughly performed and ecotoxicological data are available in the literature, a systematic analysis of their ecotoxicological potential has yet to be carried out. To fill this gap, 12 different SAs were chosen for detailed analysis with the focus on different bacteria as well as non-target organisms (algae and plants). A flexible (eco)toxicological test battery was used, including enzymes (acetylcholinesterase and glutathione reductase), luminescent marine bacteria (Vibrio fischeri), soil bacteria (Arthrobacter globiformis), limnic unicellular green algae (Scenedesmus vacuolatus) and duckweed (Lemna minor), in order to take into account both the aquatic and terrestrial compartments of the environment, as well as different trophic levels. It was found that SAs are not only toxic towards green algae (EC₅₀=1.54-32.25 mg L⁻¹) but have even stronger adverse effect on duckweed (EC₅₀=0.02-4.89 mg L⁻¹) than atrazine - herbicide (EC₅₀=2.59 mg L⁻¹).

• Analyzing Cytotoxic Effects of Selected Isothiazol-3-one Biocides Using the Toxic Ratio Concept and Structure-Activity Relationship Considerations.

  Authors: Jürgen Arning, Marianne Matzke, Stefan Stolte, Frauke Nehen, Ulrike Bottin-Weber, Andrea Böschen, Salha Abdulkarim, Bernd Jastorff, Johannes Ranke

  Chemical research in toxicology. 11/2009;

  To demonstrate how baseline toxicity can be separated from other more specific modes of toxic action and to address possible pitfals when dealing with hydrophobic substances, the fourTo demonstrate how baseline toxicity can be separated from other more specific modes of toxic action and to address possible pitfals when dealing with hydrophobic substances, the four isothiazol-3-one biocides N-methylisothiazol-3-one (MIT), 5-chloro-N-methylisothiazol-3-one (CIT), N-octylisothiazol-3-one (OIT), and 4,5-dichloro-N-octylisothiazol-3-one (DCOIT) as an example for reactive electrophilic xenobiotics were tested for their cytotoxic effects on the human hepatoblastoma cell line Hep G2, on the marine bacterium Vibrio fischeri, and on the limnic green alga Scenedesmus vacuolatus. In each of the three test systems, toxic effects were observed in a consistent pattern. The two chlorinated compounds and OIT were found to be significantly more toxic than MIT. As compared to baseline toxicants, the small and polar MIT and CIT exhibited pronounced excess toxicity in each of the three test systems that is presumably triggered by their intrinsic reactivity toward cellular thiols. In contrast, OIT and DCOIT showed mainly toxicities that could be explained by their hydrophobicity. Analyzing and comparing these results using the toxic ratio concept and with data that indicate a dramatic depletion of cellular glutathione levels after incubation with DCOIT reveals that for highly hydrophobic substances, baseline level toxicity in an assay for acute toxicity can lead to an oversight of other more specific modes of toxic action that may cause significant effects that might be less reversible than those caused by unreactive baseline toxicants. This possibility should be taken into account in the hazard assessment of chemicals that are both hydrophobic and reactive.

• Sorption and desorption of imidazolium based ionic liquids in different soil types.

  Authors: Marianne Matzke, Karen Thiele, Anja Müller, Juliane Filser

  Chemosphere. 11/2008;

  This study investigates the influence of the two different clay minerals kaolinite and smectite as well as of organic matter on the cation sorption and desorption behaviour of three imidazolium basedThis study investigates the influence of the two different clay minerals kaolinite and smectite as well as of organic matter on the cation sorption and desorption behaviour of three imidazolium based ionic liquids -1-butyl-3-methyl-imidazolium tetrafluoroborate (IM14 BF(4)), 1-methyl-3-octyl-imidazolium tetrafluoroborate (IM18 BF(4)) and 1-butyl-3-methyl-imidazolium bis[(trifluoromethyl)sulfonyl]imide (IM14 (CF(3)SO(2))(2)N) - in soil. The German standard soil Lufa 2.2 - a natural soil classified as a loamy sand - was the basis substrate for the different soil compositions and also served as a reference soil. The addition of organic matter and clays increases the sorption of the substances and in particular smectite had striking effects on the sorption capacity for all three ionic liquids indicating that ionic interactions play an important role for sorption and desorption processes of ionic liquids in soil. One exception was for kaolinite-containing soils and the IM14 cation: with (CF(3)SO(2))(2)N(-) as an anion the sorption was identical at either 10wt% or 15wt% clay content, and with BF(4)(-) sorption was even lower at 15wt% kaolinite than at 10wt%. Desorption was weak for IM18 BF(4), presumably owing to the longer alkyl side chain. With regard to the influence of kaolinite on desorption, the same pattern was observed as it was found for the sorption of IM14 BF(4) and IM14 (CF(3)SO(2))(2)N.

• Ionic liquids in soils: effects of different anion species of imidazolium based ionic liquids on wheat (Triticum aestivum) as affected by different clay minerals and clay concentrations.

  Authors: Marianne Matzke, Stefan Stolte, Jürgen Arning, Ute Uebers, Juliane Filser

  Ecotoxicology (London, England). 11/2008;

  This study contributes to a prospective hazard assessment of ionic liquids, focusing on the terrestrial environment. The influence of differently composed soils-varying contents of the clay mineralsThis study contributes to a prospective hazard assessment of ionic liquids, focusing on the terrestrial environment. The influence of differently composed soils-varying contents of the clay minerals smectite and kaolinite-on the toxicity of different anion species of imidazolium based ionic liquids was studied for growth inhibition of wheat (Triticum aestivum). IM14 (CF(3)SO(2))(2)N appeared the most toxic, independently of the investigated soil type. The toxicity of IM14 Cl, IM14 BF(4) and IM14 HSO(4) was mainly dominated by the cationic moiety. The observed effects varied in dependence of the added clay type and clay concentration. An increase of clay content resulted in less pronounced effects of these substances. In contrast, for IM14 (CF(3)SO(2))(2)N the addition of clay minerals caused higher toxic effects in comparison to the reference soil. Our results give first hints for the assumption, that ionic liquids whose toxic action is based on the anionic moiety are especially hazardous for soils, particular for soils with high clay contents.

• Structure-activity relationships for the impact of selected isothiazol-3-one biocides on glutathione metabolism and glutathione reductase of the human liver cell line Hep G2.

  Authors: Jürgen Arning, Ralf Dringen, Maike Schmidt, Anette Thiessen, Stefan Stolte, Marianne Matzke, Ulrike Bottin-Weber, Birgit Caesar-Geertz, Bernd Jastorff, Johannes Ranke

  Toxicology. 05/2008; 246(2-3):203-12.

  To investigate the toxic mode of action of isothiazol-3-one biocides the four compounds N-methylisothiazol-3-one (MIT), 5-chloro-N-methylisothiazol-3-one (CIT), N-octylisothiazol-3-one (OIT) andTo investigate the toxic mode of action of isothiazol-3-one biocides the four compounds N-methylisothiazol-3-one (MIT), 5-chloro-N-methylisothiazol-3-one (CIT), N-octylisothiazol-3-one (OIT) and 4,5-dichloro-N-octylisothiazol-3-one (DCOIT) were purified and tested as single chemical entities for their effects on the human hepatoblastoma cell line Hep G2 and on isolated and cellular glutathione reductase GR). The two chlorinated substances CIT and DCOIT significantly decreased the amount of total cellular glutathione (GSx) in a dose and time dependent manner. Concomitantly, an increase in the level of oxidised glutathione (GSSG) was observed. The resulting shift in the GSH/GSSG ratio entailing the breakdown of the cellular thiol reduction potential was accompanied by necrotic morphological changes like swelling of the plasma membrane and subsequent lysis of the cells. Additionally, CIT and DCOIT were found to inhibit cellular GR in the cells in a concentration dependent manner. The T-SAR-based (thinking in terms of structure-activity relationships) comparison of the chlorine-substituted structures CIT and DCOIT with their non-chlorinated and less active analogues MIT and OIT identified the chlorine substituents and the resulting reaction mechanisms to be the key structural mediators of the observed toxic effects. Furthermore, differences in the activity of both chlorinated substances could be explained using the T-SAR approach to link the lipophilicity and the intrinsic glutathione-reactivity of the compounds to the expected target site concentrations inside the cells.

• Heavy metal toxicity to Lemna minor: studies on the time dependence of growth inhibition and the recovery after exposure.

  Authors: Wiebke Drost, Marianne Matzke, Thomas Backhaus

  Chemosphere. 03/2007; 67(1):36-43.

  Environmental concentrations of toxic substances are not necessarily constant but fluctuate over time. Periods of intense exposure might be followed by episodes with a relatively low or no exposure,Environmental concentrations of toxic substances are not necessarily constant but fluctuate over time. Periods of intense exposure might be followed by episodes with a relatively low or no exposure, in principle allowing exposed organisms to recover from toxic injury. The growth reproduction assay with the limnic vascular plant Lemna minor allows for convenient studies on the time dependence of the aquatic toxicity of chemicals. Here we report on a study with four priority metals (Zn, Cu, Ni, Cd). Aims of the study were to determine the impact of the exposure duration on the observed toxicities and to determine the potential for recovery. The bioconcentrations of the test metals were recorded during the exposure in order to analyse, whether changes in the internal concentrations are a governing factor for the dynamics of toxicity. After an exposure of 7 days, Cd and Cu showed the highest toxicity to Lemna (EC50's of 1.9 and 9.7 microM respectively), while Ni and Zn had a slightly lower toxicity (EC50's of 56.3 and 46.1 microM respectively). Additionally, Zn showed a severely delayed toxicity and the exposed plants did not recover even 7 days after the exposure had ended. This is in sharp contrast to the other test metals, for which a considerable recovery was observed. These results indicate the necessity to more thoroughly consider the dynamics of toxicity, instead of recording toxic effects only after a constant exposure over a fixed time.

• Heavy metal toxicity to Lemna minor: studies on the time dependence of growth inhibition and the recovery after exposure

  Authors: Wiebke Drost, Marianne Matzke, Thomas Backhaus

  Chemosphere.

  Environmental concentrations of toxic substances are not necessarily constant but fluctuate over time. Periods of intense exposure might be followed by episodes with a relatively low or no exposure,Environmental concentrations of toxic substances are not necessarily constant but fluctuate over time. Periods of intense exposure might be followed by episodes with a relatively low or no exposure, in principle allowing exposed organisms to recover from toxic injury. The growth reproduction assay with the limnic vascular plant Lemna minor allows for convenient studies on the time dependence of the aquatic toxicity of chemicals. Here we report on a study with four priority metals (Zn, Cu, Ni, Cd). Aims of the study were to determine the impact of the exposure duration on the observed toxicities and to determine the potential for recovery. The bioconcentrations of the test metals were recorded during the exposure in order to analyse, whether changes in the internal concentrations are a governing factor for the dynamics of toxicity. After an exposure of 7 days, Cd and Cu showed the highest toxicity to Lemna (EC50’s of 1.9 and 9.7 μM respectively), while Ni and Zn had a slightly lower toxicity (EC50’s of 56.3 and 46.1μM respectively). Additionally, Zn showed a severely delayed toxicity and the exposed plants did not recover even 7 days after the exposure had ended. This is in sharp contrast to the other test metals, for which a considerable recovery was observed. These results indicate the necessity to more thoroughly consider the dynamics of toxicity, instead of recording toxic effects only after a constant exposure over a fixed time.

• Sorption and desorption of imidazolium based ionic liquids in different soil types

  Authors: Marianne Matzke, Karen Thiele, Anja Müller, Juliane Filser

  Chemosphere.

  This study investigates the influence of the two different clay minerals kaolinite and smectite as well as of organic matter on the cation sorption and desorption behaviour of three imidazolium basedThis study investigates the influence of the two different clay minerals kaolinite and smectite as well as of organic matter on the cation sorption and desorption behaviour of three imidazolium based ionic liquids -1-butyl-3-methyl-imidazolium tetrafluoroborate (IM14 BF4), 1-methyl-3-octyl-imidazolium tetrafluoroborate (IM18 BF4) and 1-butyl-3-methyl-imidazolium bis[(trifluoromethyl)sulfonyl]imide (IM14 (CF3SO2)2N) – in soil. The German standard soil Lufa 2.2 – a natural soil classified as a loamy sand – was the basis substrate for the different soil compositions and also served as a reference soil. The addition of organic matter and clays increases the sorption of the substances and in particular smectite had striking effects on the sorption capacity for all three ionic liquids indicating that ionic interactions play an important role for sorption and desorption processes of ionic liquids in soil. One exception was for kaolinite-containing soils and the IM14 cation: with (CF3SO2)2N− as an anion the sorption was identical at either 10 wt% or 15 wt% clay content, and with BF4− sorption was even lower at 15 wt% kaolinite than at 10 wt%. Desorption was weak for IM18 BF4, presumably owing to the longer alkyl side chain. With regard to the influence of kaolinite on desorption, the same pattern was observed as it was found for the sorption of IM14 BF4 and IM14 (CF3SO2)2N.

• Behaviour and toxicity of imidazolium based ionic liquids in soils

  Authors: Marianne Matzke

  http://elib.suub.uni-bremen.de/diss/docs/00011019.pdf.

  This study contributes to a prospective hazard assessment of ionic liquids (ILs) for the terrestrial environment by analysing the (bio)availability (and therefore toxicity) of selected imidazoliumThis study contributes to a prospective hazard assessment of ionic liquids (ILs) for the terrestrial environment by analysing the (bio)availability (and therefore toxicity) of selected imidazolium based ILs in dependence on the soil properties organic matter, clay type and clay content. In general this hazard assessment is complicated by the structural heterogeneity within the substance class of ionic liquids which provides an almost unlimited number of theoretically available chemical structures. For this reason a tiered testing strategy was used to efficiently analyse the hazard potential of selected ILs starting with theoretical assumptions according to the T-SAR (Thinking in Terms of Structure Activity Relationships) concept followed by a set up of different test kits. Those assumptions were experimentally validated with an (eco)toxicological test battery and followed by a closer analysis of the (bio)availability of selected ILs. The bioavailability was assessed by using sorption/desorption studies as well as phytotoxicity assays. Additionally the issue of mixture toxicity was analysed for ILs mixtures in the presences and absence of the heavy metal cadmium.