[Show description][Hide description] DESCRIPTION: Background: Mantle cell lymphoma (MCL) is an aggressive B-cell lymphoid malignancy that accounts for approximately 6% of all non-Hodgkin´s lymphomas. SOX4, SOX11 and SOX12 genes, constitute the SOXC family of transcription factors involved in embryonic neurogenesis and tissue remodeling. Among them, SOX11 shows aberrant expression in
MCL, being considered a new molecular marker of adverse prognosis in this pathology; meanwhile recently it has shown that SOX4 can bind and regulate the promoter of Dicer, a microRNA biogenesis factor. Furthermore, several studies have demonstrated the oncogenic role of miR-17-92 cluster in hematological malignancies, being scarce the information about the association between this cluster and SOXC expression levels in MCL.
Aims: In this study, we have performed a Gene Expression Analysis (GEA) of SOXC cluster and their correlation with the expression of miR-17, miR18a, miR19b and miR92a, members of the polycistronic oncomiR-17-92 in MCL patients.
[Show abstract][Hide abstract] ABSTRACT: To study the main clinical and histopathological features of 12 patients with Hodgkin's lymphoma (HL) diagnosed primarily from bone marrow (BM) involvement.
We included 12 acquired immunodeficiency syndrome (AIDS) patients with HL assisted in the F. J. Muñiz Infectious Diseases Hospital since January 2002 to December 2013. The diagnosis of HL with primary BM involvement in patients was confirmed by clinical, histopathological, and immunohistochemical findings.
All patients presented "B" symptoms and pancytopenia. All of them had stage IV neoplasm disease because of BM infiltration. The median of CD4(+) T-cell counts was 114 cells/μL, and mixed cellularity (MC) was the most frequent histopathological subtype of 92% cases.
When other causes are excluded, BM biopsy should be performed in AIDS patients with "B" symptoms and pancytopenia to evaluate BM infiltration by atypical lymphocytes.
Cancer Biology and Medicine 03/2015; 12(1):41-5. DOI:10.7497/j.issn.2095-3941.2014.0024
[Show abstract][Hide abstract] ABSTRACT: Introduction Plasmablastic lymphoma is a rare entity that was first described in the jaws and the oral cavity of patients with human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS). Plasmablastic lymphoma is considered as a diffuse, large, B-cell lymphoma with a unique phenotype and a predilection for the oral cavity. Objective The authors describe a case of an aggressive plasmablastic lymphoma of the oral cavity as the primary manifestation of AIDS. Resumed Report We report a case of plasmablastic lymphoma involving only the oral cavity as the first manifestation of AIDS. Diagnosis was confirmed by the oral lesion biopsy and the histopathologic examination that showed a dense infiltrate composed of atypical lymphocytes with numerous plasmocytes that expressed the plasma cell markers MUM-1 and CD138 and that were negative for the B-cell markers CD3, CD20, and CD45. Immunohistochemical and in situ hybridization revealed the Epstein-Barr virus genome in the atypical cells. Polymerase chain reaction was also positive for human herpesvirus-8 RNA. Conclusion The HIV serologic status should be evaluated in all patients with plasmablastic lymphoma of the oral cavity or extraoral sites.
International Archives of Otorhinolaryngology 01/2015; 19(4). DOI:10.1055/s-0034-1397335
[Show abstract][Hide abstract] ABSTRACT: Introduction Extranodal non-Hodgkin lymphomas (NHLs) are commonly described in patients with acquired immunodeficiency syndrome (AIDS) and are related with an atypical morphology and aggressive clinical course. AIDS-associated lymphomas are characterized by their rapid progression, frequent extranodal manifestations, and poor outcome. Objective The aim of this article is to remake the clinical features of head and neck (HN) NHL in patients with AIDS to facilitate early diagnosis and treatment. Methods We evaluated the epidemiologic, clinical, immunologic, virologic, and histopathologic characteristics of 24 patients with human immunodeficiency virus (HIV)/AIDS with primary HN NHL treated at a single institution between 2002 and 2012. Histopathologic diagnosis was made according to the criteria of the World Health Organization Classification of Tumors of Hematopoietic and Lymphoid Tissues. Additional immunohistochemical stains were applied in all cases. Results Eighteen patients (75%) were men and the median of age was 39 years. The gingiva and the hard palate were the most common sites of the lesions (15 patients, 62.5%). Lactate dehydrogenase levels were elevated in 16 cases (84%). Bone marrow infiltration was detected only in 4 cases (16.6%). The median CD4 T-cell count was 100 cells/µL. According to the histopathologic evaluation, the most common subtype was diffuse large B-cell lymphoma (12 cases, 50%), followed by plasmablastic lymphoma (9 cases, 37.5%) and Burkitt lymphoma (3 cases, 12.5%). Conclusion HN NHL is a severe complication of advanced HIV/AIDS disease. Early diagnosis followed by chemotherapy plus highly active antiretroviral treatment is necessary to improve the prognosis and the survival of these patients.
International Archives of Otorhinolaryngology 07/2014; 18(03):260-265. DOI:10.1055/s-0034-1373782
[Show abstract][Hide abstract] ABSTRACT: Non-Hodgkin lymphomas (NHL) are a heterogeneous group of diseases with a high prevalence in human immunodeficiency virus (HIV) infected patients. The immunosuppression associated with AIDS predisposes to develop NHL, including Burkitt's lymphoma (BL). BL is an uncommon and aggressive subtype of NHL that occurs with increased frequency among patients with AIDS. BL is associated with a high proliferative rate (Ki67 index) and compromises extranodal sites as the clinical presentation of the disease. Here we report a case of a primary BL of the oral cavity in an AIDS female patient, and a review the literature on the characteristics of oral cavity lymphomas in AIDS patients.
Revista Espanola de Cirugia Oral y Maxilofacial 01/2014; 37(1). DOI:10.1016/j.maxilo.2013.04.005
[Show abstract][Hide abstract] ABSTRACT: Primary soft tissue Non-Hodgkin lymphomas are very rare and account only for 0.1 % of
the cases. Generally, Non-Hodgkin lymphomas of the soft tissue present as large
subcutaneous masses without evidence of nodal or skin involvement. We describe four
cases of primary Non-Hodgkin lymphomas of the soft tissue in patients infected with
the human immunodeficiency virus. The most common site of involvement was the chest
wall in all the patients; histopathological and immunophenotypic examination of the
biopsy smears revealed two cases of plasmablastic lymphomas, one Burkitt and one
diffuse large B-cell lymphoma. Non-Hodgkin lymphomas should be included in the
differential diagnosis of soft tissue masses in human immunodeficiency virus -
[Show abstract][Hide abstract] ABSTRACT: Plasmablastic lymphoma (PBL) is a B-cell–derived lymphoma that is described as a subtype of the diffuse large B-cell lymphoma, commonly seen in patients with human immunodeficiency virus infection. The oral cavity is the first site of presentation with local invasion to the palate and the jaw and rapid dissemination to extraoral sites. The characteristic immunophenotype include the presence of plasma cell markers VS38c and CD138. Epstein-Barr virus and human herpes virus type-8 are strongly associated with the pathogenesis of oral PBL. Here, we describe a case of PBL of the oral cavity in a 55-year-old woman who was only treated with HAART and improved with a full remission of the neoplasm, which is probably related with a good control of the immunological and virological status associated with the highly active antiretroviral therapy.
Infectious Disease in Clinical Practice 01/2012; 20(1):79-81. DOI:10.1097/IPC.0b013e31821618a4
[Show abstract][Hide abstract] ABSTRACT: Extranodal non-Hodgkin lymphoma (NHL) were commonly described in AIDS patients and are related with an atypical morphology and aggressive clinical course.
In this single institutional study we evaluated the epidemiological, clinical, immunological, virological, histopathological and the outcome of eleven HIV/AIDS patients with oral cavity lymphomas (OCL).
Nine were males and seven intravenous drug abusers. The median of age was 33 years and the median of CD4 T cell counts at the time of diagnosis was 97 cell/µL. The majority of tumors presented as large and ulcerated masses involving the gingiva, the palate and the jaw. Six of these tumors were diffuse large B-cell lymphomas (DLBCL); three were Burkitt's lymphomas and the final case was a plasmablastic lymphoma. An association with Epstein-Barr virus (EBV) was found in three of the ten tested cases by in situ hybridization (EBER 1 and 2 probes) and immunohistochemistry (LMP-1). Human herpes virus-8 (HHV-8) was detected by polymerase chain reaction (PCR) in only one neoplasm. Six patients died without specific treatment; four received chemotherapy and highly active antiretroviral therapy (HAART) and three of them presented a prolonged survival.
Combination of HAART and chemotherapy should modify the poor prognosis of AIDS patients with OCL.
Journal of Gastrointestinal Cancer 09/2011; 42(3):143-8. DOI:10.1007/s12029-010-9173-9 · 0.38 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Cutaneous B cell lymphoma (CBCL) is a lymphoproliferative disorder of neoplastic B cell of the skin with a wide range of clinical manifestations. Commonly, the clinical features of CBCL are plaques, nodules, or ulcerative lesions. Skin is one of the common sites for extra-nodal lymphomas in patients with AIDS and B cell type is less common than T cell type. Only recently, the existence of B cell lymphomas presenting clinically in the skin without evidence of extra-cutaneous involvement has been accepted as primary CBCL. Here, we are presenting 5 patients with cutaneous involvement in the setting of HIV/AIDS disease. Two of them were primary cutaneous non-Hodgkin lymphomas. All were CBCL; 3 were immunoblastic, 1 was plasmablastic, and the other was a Burkitt lymphoma. We analyzed the epidemiological, clinical, virological, and immunological characteristics of this group of patients.
The Brazilian journal of infectious diseases: an official publication of the Brazilian Society of Infectious Diseases 02/2010; 14(1):81-5. DOI:10.1590/S1413-86702010000100016 · 1.30 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Non-Hodgkin lymphomas (NHL) of the B-cell type are the second most common neoplasm among patients with human immunodeficiency virus (HIV) infection and AIDS. Here, we evaluated 48 cases of AIDS-related lymphomas (ARL) diagnosed at the Histopathological Division of the Instituto de Investigaciones Hematológicas of the National Academy of Medicine. Five were females and 43 were males with a median of age of 37 years at the time of the diagnosis. Micrometer sections were prepared and stained with hematoxilin-eosin; immunohistochemical examination for the presence of Epstein-Barr virus (EBV) was carried out in 48/48 cases. Additionally, biotinilated oligonucleotides were used to determine the presence of DNA of the Human Herpes virus type-8 (HHV-8) in 14/14 biopsy smears corresponding to plasmablastic lymphomas (PL). All were fenotype B cell lymphomas with an aggressive course and advanced neoplasm disease at the time of diagnosis. Virological findings showed the strong association between EBV and AIDS-related NHL. According to the histopathological subtype, the EBV genome was detected in 16/21 (76%) diffuse large B cell lymphomas, 1/3 Burkitt lymphoma and 3/4 (75%) of primary central nervous system lymphomas. Globally, EBV genome was detected in 20/28 NHL of this series. Detection of HHV-8 was negative in all cases of PL. Hodgkin lymphoma were more frequent in males 18/20 (90%), with an aggressive clinical course and a significant predominance of the subtypes associated with worse prognosis (90% of cases). We detected a significant association between EBV and HL (90% of cases). We consider that all cases of AIDS related lymphomas should be assessed for the presence of EBV because its presence may play a role in the prognosis.
[Show abstract][Hide abstract] ABSTRACT: Mantle cell lymphoma (MCL) is a well defined lymphoid neoplasm genetically characterized by the t(11;14)(q13;q32). Telomeres play an essential role in preserving chromosomal integrity and genomic stability; their shortening can lead to telomere dysfunction and chromosomal instability, a critical factor in cancer development. In this study, telomere length (TL) measured by terminal restriction fragments (TRF) assay in DNA samples of tumor cells from 20 patients with MCL was evaluated. Results were correlated with clinical, morphologic and cytogenetic characteristics. In all cases, the presence of the CCND1/IGH@ rearrangement was confirmed by fluorescence in situ hybridization and/or PCR analysis. TL in total MCL patients revealed a mean TRF value (4.51 +/- 0.79 kb) significantly shorter than those observed in controls (7.49 +/- 1.94 kb) (P < 0.001); 30% of patients had TL shorter than 4.0 kb. TRF length was not associated with patients age (P = 0.07; r = 0.17) nor with sex (females: 4.33 +/- 0.51 kb and males: 4.57 +/- 0.85 kb; P = 0.63). No significant differences were found between patients studied at diagnosis (13) (4.44 +/- 0.81 kb) respect to those analyzed at relapse (7) (4.63 +/- 0.82 kb) (P = 0.53). In addition, we compared patients with (4.84 +/- 1.09 kb) and without (4.40 +/- 0.68 kb) complex karyotypes (P = 0.45) and cases with typical morphology (4.48 +/- 0.79 kb) vs. blastoid variant (4.63 +/- 1.04 kb) (P = 0.83), and no significant differences between them were found. Although the number of cases of our series is not large, our results showed that TL reduction in MCL is independent of the clinical characteristics, morphology and karyotype.
European Journal Of Haematology 07/2009; 83(5):433-8. DOI:10.1111/j.1600-0609.2009.01313.x · 2.07 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Plasmablastic lymphoma is a rare and a relatively new entity that was first described in the jaws and the oral cavity of HIV-AIDS patients. We report a case of plasmablastic lymphoma involving the liver in an AIDS patient. Plasmablastic lymphoma is considered a diffuse large B-cell lymphoma with a unique phenotype and predilection for the oral cavity. The case presented had a unique hepatic lesion, localized in the left lobe of the liver. Diagnosis was confirmed by hepatic biopsy guided by Computerized Tomography scan and histopathology. The smears showed a dense infiltrate composed by atypical lymphocytes with numerous plasmocytes expressing the plasma cell markers MUM-1 and CD138 and negative for the B-cell markers CD3, CD20 and CD45. Immunohistochemical and in situ hybridization revealed the Epstein-Barr virus genome in the atypical cells. Polymerase chain reaction was negative for HHV-8 RNA.
Annals of hepatology: official journal of the Mexican Association of Hepatology 07/2009; 8(3):242-5. · 2.07 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Smooth muscle neoplasms are more frequent in human immunodeficiency infected children than in HIV seropositive adults. Endobronchial leiomyoma is a rare benign tumor in HIV infected adult patients. Epstein-Barr virus (EBV) has been implicated in the pathogenesis of these tumors. Here we describe an adult patient with HIV infection with atelectasis of the left upper pulmonary lobe as the first clinical expression of an intrabronchial leiomyoma. In this case, we can not show the association with EBV. Our report suggests that smooth muscle tumors as leiomyoma should be included in the differential diagnosis of endobronchial masses in AIDS patients.
Revista do Instituto de Medicina Tropical de São Paulo 02/2009; 51(1):53-5. DOI:10.1590/S0036-46652009000100010 · 1.01 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Morphological, immunophenotypic and genetic heterogeneity amongst mantle cell lymphomas (MCLs) can lead to difficulties in diagnosis and management. The aim was to describe the clinical and pathological features of MCLs with aberrant expression of CD10.
Of 17 specimens from 13 patients, 14 expressed CD10 and three (presenting before or after a CD10+ specimen) did not. All expressed cyclin D1 and carried the t(11;14)(q13;q32)/CCND1-IGH translocation. Similar to non-selected MCL patients, most patients had disseminated disease and an adverse clinical course. Five specimens showed pleomorphic blastoid morphology and blastoid transformation was associated with a change in phenotype, including gain or loss of CD10. Additional phenotypic variations likely to cause diagnostic difficulty were present in eight specimens: five were CD5- and five (all CD10+) expressed Bcl-6. One Bcl-6+ case carried a BCL-6 translocation and three others had extra copies of the BCL-6 gene. Sequence analysis of the immunoglobulin heavy chain variable region in five cases showed only one to have low-level somatic mutation, indicating that they did not arise from germinal centre B cells.
Expression of CD10 by MCL is often associated with other variant morphological, immunophenotypic or genetic features, but does not reflect derivation from germinal centre B cells.
[Show abstract][Hide abstract] ABSTRACT: Adult intussusception is rare. Here, we describe a case of an AIDS adult patient who developed an ileocolic intussusception secondary to a large B cell lymphoma of the cecum. Surgical findings included the ileon free of the tumor and invaginated within the cecum with infiltrating neoplasm. Surgical treatment included the resection of the right hemicolon because of the tumor, located in the cecum, causing intussusception. The English and Spanish literature is reviewed.
[Show abstract][Hide abstract] ABSTRACT: A retrospective study was conducted of extranodal oral Non-Hodgkin's Lymphomas diagnosed at the Surgical Pathology Laboratory of the School of Dentistry at Buenos Aires University, Argentina, between 1985 and 2004. The 40 cases found represent 0.2% of the oral biopsies diagnosed during that time and 4.6% of malignant neoplasias. Overall mean age of patients was 49.4 years, and frequency was greater in males. 80% affected soft tissues. Prevalent location was gingival, followed by palate. Intraosseous cases were more frequent in mandible (75%) than in upper maxilla. 100% of the cases were phenotype B, with a higher frequency of high-grade aggressiveness. The most common histological type was Diffuse Large Cell Lymphoma. 60% of the Plasmablastic Lymphomas in the series came from HIV+ patients. Evolution time prior to consultation was 1 to 3 months in 57.7% of the cases.