Jiye Cai

Jinan University (Guangzhou, China), Shengcheng, Guangdong, China

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Publications (117)298.86 Total impact

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    ABSTRACT: A rapid, facile assay for sensitive cytosensing of breast cancer cells should help to guide potential medical evaluation for breast cancer. Here, we report development of novel resonance Rayleigh scattering (RRS) cytosensor for cell recognitions and folate (FA) receptor expression analyses on living cells. Using FA-conjugated gold nanoparticles (FA-AuNPs) as nanoprobes, the constructed nanoprobes-assembled recognition interface could increase the binding capacity for cell recognition, amplify Au-aggregates-enhanced RRS signal, and then enhance the sensitivity for membrane antibody assay. FA-AuNPs-based RRS measurements enabled a distinct 34-times-enhancement in RRS intensities after incubation with human breast cancer cells, compared with normal cells. Receptor-targeted cytosensor was used to quantitatively detect human breast cancer MCF-7, liver cancer HepG2 and normal cells, which expressing different amount of FA receptor, respectively. The detection limit for MCF-7 cells was 12 cells/mL with good selectivity and reproducibility. Furthermore, the proposed cytosensor allowed for dynamic evaluation of FA receptor expression on different living cells after dihydroartemisinin stimulus. This assay platform shows the good potential for clinical diagnostics and antibody-targeted drug screening. Copyright © 2015 Elsevier B.V. All rights reserved.
    Biosensors & Bioelectronics 12/2015; 74. DOI:10.1016/j.bios.2015.06.012 · 6.45 Impact Factor
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    ABSTRACT: Quercetin, a wildly distributed bioflavonoid, has been proved to possess excellent antitumor activity on hepatocellular carcinoma (HCC). In the present study, the biophysical properties of HepG2 cells were qualitatively and quantitatively determined using high resolution atomic force microscopy (AFM) to understand the anticancer effects of quercetin on HCC cells at nanoscale. The results showed that quercetin could induce severe apoptosis in HepG2 cells through arrest of cell cycle and disruption of mitochondria membrane potential. Additionally, the nuclei and F-actin structures of HepG2 cells were destroyed by quercetin treatment as well. AFM morphological data showed some typical apoptotic characterization of HepG2 cells with increased particle size and roughness in the ultrastructure of cell surface upon quercetin treatment. As an important biophysical property of cells, the membrane stiffness of HepG2 cells was further quantified by AFM force measurements, which indicated that HepG2 cells became much stiffer after quercetin treatment. These results collectively suggest that quercetin can be served as a potential therapeutic agent for HCC, which not only extends our understanding of the anticancer effects of quercetin against HCC cells into nanoscale, but also highlights the applications of AFM for the investigation of anticancer drugs. SCANNING 9999:1-13, 2015. © 2015 Wiley Periodicals, Inc. © Wiley Periodicals, Inc.
    Scanning 07/2015; DOI:10.1002/sca.21245 · 1.44 Impact Factor
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    ABSTRACT: Water-soluble germanium-doped cadmium sulfide quantum dots (Ge:CdS q-dots) were successfully synthesized by a facile one-pot method, and used as fluorescent probes for labeling live MCF-7 cells. The optical properties of the q-dots and several key parameters of the synthesis conditions were studied, and the mechanism of these parameters was also discussed. After doping with germanium, the PL intensity increased 50%, while improving the photostability and fluorescence quantum yield. Furthermore, the toxicity of CdS quantum dot decreased after doping with germanium due to its low toxicity, which greatly improved the CdS quantum dots biocompatibility. Then the doped quantum dots modified with folic acid (FA) to use as fluorescent probes, which expanded the application of semiconductor quantum dots in the biomedical field.
    Materials Science in Semiconductor Processing 06/2015; 34. DOI:10.1016/j.mssp.2015.01.024 · 1.76 Impact Factor
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    ABSTRACT: Quartz crystal microbalance with dissipation monitoring (QCM-D) was used for real-time and label-free detection of changes and folate receptor (FR) expression levels on living MCF-7 cells for evaluating the anticancer activity of resveratrol. Here, the mechanical changes of cellular responses to resveratrol was tracked by poly(L-lysine) (PLL) modified QCM-D sensor and the inhibition effect of resveratrol on FR expression levels on MCF-7 cells was monitored by chitosan-folic acid (CS-FA) composite membrane functionalized Au substrate for the first time. Changes in morphology and cellular state of MCF-7 cell stimulated by resveratrol at different concentrations were detected by inverted fluorescence microscope and flow cytometry. Atomic force microscopy confirmed that resveratrol influenced the cellular mechanical properties. The results indicated that the MCF-7 cells lose its original elasticity and increase its stiffness induced by resveratrol. Confocal fluorescence imaging further observed that resveratrol reduced the FR expression levels on the living cells surface. This study established a typical model of QCM-D biosensor to evaluate the protein biomarker expression levels on cells surface. QCM-D, which was used to investigate potential targets for anti-tumor drug on living cells and realize a better understanding of drug action mechanism, was expected to be developed into a promising tool for screening of drugs.
    Analytical Chemistry 04/2015; 87(9). DOI:10.1021/acs.analchem.5b00083 · 5.83 Impact Factor
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    ABSTRACT: Colorectal cancer, a kind of malignant cancer, has more than 1 million new patients and results in 0.5 million deaths every year globally based on the estimation of Globocan in 2008. One of the most important issues against colon cancer is tumor metastasis. Anti-angiogenesis, a form of targeted therapy uses drugs or other substances to prevent the new blood vessel formation, which is critical for tumor metastasis. In our previous studies, we have demonstrated a simple method to synthesize Chry-Ge complex through the reaction between chrysin and triphenylgermanium bromide. In this work, we investigated the mechanism of Chry-Ge induced Colo205 cell apoptosis. We found that Chry-Ge could induce apoptosis in Colo205 cells in mitochondrial-dependent pathway, cause the reorganization of cytoskeleton and induce the damage of nucleus in Colo205 cells. Besides, Chry-Ge was also found to induce membrane ultrastructural changes in Colo205 cells by AFM. Further, we found that Chry-Ge can inhibit tube formation of human umbilical vascular endothelial cell in vitro. Chry-Ge was also tested in vivo in the chicken chorioallantoic membrane (CAM) assay and found to inhibit bFGF-treated CAMs development. These results suggested that Chry-Ge could induce Colo205 cell apoptosis by mitochondrial pathway and anti-angiogenesis, highlighting the use of organic germanium agents for the treatment of colorectal cancer. SCANNING 9999:1-12, 2015. © 2015 Wiley Periodicals, Inc. © Wiley Periodicals, Inc.
    Scanning 04/2015; DOI:10.1002/sca.21205 · 1.44 Impact Factor
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    ABSTRACT: The migration of mesenchymal stem cells (MSCs) plays a key role in tumor-targeted delivery vehicles and tumor-related stroma formation. However, there so far has been no report on the distribution of cell surface molecules during the VEGF-induced migration of MSCs. Here, we have utilized near-field scanning optical microscopy (NSOM) combined with fluorescent quantum dot (QD)-based nano-technology to capture the functional relationship between CD44 and CD29 adhesion molecules on MSCs and the effect of their spatial rearrangements. Before VEGF-induced migration of MSCs, both CD44 and CD29 formed 200-220 nm nano-domains respectively, with little co-localization between the two types of domains. Surprisingly, the size of the CD44 nano-domain rapidly increased in size to 295 nm and apparently larger aggregates were formed following MSC treatment with VEGF for 10 min, while the area of co-localization increased to 0.327 mu m(2). Compared with CD44, CD29 was activated obviously later, for the fact that CD29 aggregation didn't appear until 30 min after VEGF treatment. Consistently, its co-localization area increased to 0.917 mu m(2). The CD44 and CD29 nano-domains further aggregated into larger nano-domains or even formed micro-domains on the membrane of activated MSCs. The aggregation and co-localization of these molecules promoted FAK formation and cytoskeleton rearrangement. All of the above changes induced by VEGF contributed to MSC migration. Taken together, our data of NSOM-based dual color fluorescent imaging demonstrated for the first time that CD44, together with CD29, involved in VEGF-induced migration of MSCs through the interaction between CD44 and its co-receptor of VEGFR-2.
    Biochimica et Biophysica Acta (BBA) - Biomembranes 12/2014; 1848(3). DOI:10.1016/j.bbamem.2014.12.013 · 3.43 Impact Factor
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    ABSTRACT: Nanoparticle aggregates induced by synergistic effect of electrostatic interaction and hydrogen-bonding recognition between melamine and 3-mercaptopriopionic acid (MA), which conjugated on the surface of gold nanoparticles (AuNPs) are used for detection of melamine. MA molecules are conjugated on AuNP surfaces to form MA-modified AuNPs (MA-AuNPs), acting as nanoprobes in the detection of melamine. Since such nanoparticle aggregates-mediated signal amplification can be measured by absorption spectroscopy, the method enables sensitive and real-time detection of melamine at the detection limit as low as 0.4 μg/ml, and the linear detection ranging from 0.6 μg/ml to 42 μg/ml. The color change can be readily seen by the naked eye at 30 μg/ml melamine without the aid of any advanced instruments. This method has been successfully applied to detect melamine in infant formula with good reproducibility, and it has the potential of being used in food safety detection.
    Journal of Food Engineering 12/2014; 142:163–169. DOI:10.1016/j.jfoodeng.2014.04.018 · 2.58 Impact Factor
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    ABSTRACT: A highly sensitive and recyclable quartz crystal microbalance (QCM) biosensor was developed using chitosan (CS) and folic acid (FA), generating conjugates that are selectively recognized by MCF-7 cancer cell over-expressed folic acid receptors. The prepared CS–FA conjugate was characterized by UV-vis spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy and scanning electron microscopy further presented the morphology of the CS–FA conjugate interface. The hydrophilicity of films was characterized by measuring the contact angle. The recognition of MCF-7 cancer cells was investigated in situ using QCM. Captured by FA, the concentration of the MCF-7 cell was determined on-line using a quartz crystal microbalance and a wide linear range of 4.5 × 102 to 1.01 × 105 cells per mL was obtained, with a detection limit of 430 cells per mL. The fluorescence microscope further confirmed the specificity and biocompatibility of the constructed biosensor. In addition, the regeneration of the QCM biosensor was studied by using lysozyme. This receptor-bound ligand based QCM biosensor also showed good selectivity, and repeatability in the cell mixture. For the first time, this simple, economical and label-free chitosan-based QCM sensing was demonstrated, and such design could provide a promising detection strategy for sensitive detection of cancer cell over-expressed folic acid receptors.
    The Analyst 10/2014; 139(23). DOI:10.1039/C4AN01532K · 3.91 Impact Factor
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    ABSTRACT: In recent years, LPS activated RAW264.7 cells are widely used as an in vitro inflammatory model for the screen of effective anti-inflammation drugs and the investigation of exact anti-inflammation mechanism of these drugs. But up to now, there are few data about the effect of LPS on the morphology, especially on the membrane ultrastructure and bio-mechanical properties of RAW264.7 macrophages. In this work, the topographical and biophysical changes of RAW264.7 macrophages upon LPS stimulation are detected by high resolution atomic force microscopy (AFM). The AFM results suggested that LPS activated RAW264.7 macrophages changed to be much bigger than control cells with some holes emerged on cell surface. The size of membrane protein clusters and the roughness of membrane significantly increased after LPS exposure. In addition, the AFM force measurement results demonstrated that LPS stimulation increased the adhesion force of RAW264.7 macrophages, and also increased the stiffness of RAW264.7 macrophages, which were attributed to the re-distribution of intracellular F-actin structures induced by LPS. These findings suggested that LPS stimulation could also induce the pathophysiological changes of RAW264.7 macrophages, which would benefit our understanding of the inflammatory processes in macrophages upon pathogen stimulation at nano-scale.
    Micron 10/2014; 65. DOI:10.1016/j.micron.2014.03.012 · 2.06 Impact Factor
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    ABSTRACT: Apigenin is a flavonoid, which has been proved to possess effective anti-cancer bioactivities against variety of cell lines. However, little is known about its effect on the cell-surface and the interaction between cell-surface and the reacting drug. In this study, human breast cancer line (MCF-7) was selected to be as a cell model to investigate the effects of apigenin on cell growth, proliferation, apoptosis, cellular morphology, etc. MTT assay showed that the growth inhibition induced by apigenin was in a dose-dependent manner when treated with different concentrations of apigenin while had little cytotoxic effects on human normal cells (MCF-10A). Fluorescence-based flow cytometry was used to detect cellular apoptosis and ROS production. The results showed that 80 µM apigenin could effectively induce apoptosis and overproduction of ROS in MCF-7 cells. Here, atomic force microscopy (AFM) was utilized to detect the shapes and membrane structures of MCF-7 cells at cellular or subcellular level. The results showed that the control MCF-7 cells presented typical elongated-spindle shapes with abundant pseudopodia, while after treated with apigenin, the cells shrunk and became round, the pseudopodia diminished. Moreover, the images of ultrastructure indicated that the cell membrane was composed of nanoparticles of 49 nm, but with the treated concentrations of apigenin increasing, the sizes of membrane particles significantly increased to 400 nm. These results can improve our understanding of apigenin, which can be potentially developed as a new agent for treatment of cancers. SCANNING 9999:XX–XX, 2014. © 2014 Wiley Periodicals, Inc.
    Scanning 10/2014; DOI:10.1002/sca.21170 · 1.44 Impact Factor
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    ABSTRACT: Graphical abstract Combining DHA with Ge-132, we synthetized a novel organogermanium compound that possessed better antitumor activity than both DHA and Ge-132. The MTT assay was used to observe the inhibitory effects of DHA-Ge on tumor cells and flow cytomery was employed to assay apoptosis of tumor cells after treatment with DHA-Ge. Atomic force microscopy was carried out to investigate morphology of cells treated with the synthesized complex. These results suggested that the synthesized compound showed excellent antitumor activity and it may be developed to be a promising anti-tumor reagent.
    Bioorganic & Medicinal Chemistry Letters 09/2014; 24(22). DOI:10.1016/j.bmcl.2014.09.048 · 2.33 Impact Factor
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    ABSTRACT: Cell membrane, which consists of viscous phospholipid bilayer, different kinds of proteins and various nano/micro meter sized domains, plays very important roles in ensuring the stability of intracellular environment and the order of cellular signal transductions. Exploring the precise cell membrane structures and detailed functions of biomolecules in cell membrane would be helpful to understand the underlying mechanisms involved in cell membrane signal transductions and further benefit the research of cell biology, immunology and medicine. Detections of membrane biomolecules at single molecule level can provide some subtle information about molecular structure and functions of cell membrane. In particular, the molecular mechanisms and information obtained at single molecule level are significantly distinguished than that detected by a large amount of biomolecules at large-scale through traditional techniques, providing novel perspective for the study of cell membrane structures and functions. Moreover, the precise investigations of membrane biomolecules urge researchers to explore the cell membranes at single molecule level by in situ imaging methods as the exact conformation and function of biomolecules are highly controlled by the native cellular environment. Recently, in situ single molecule imaging of cell membranes has attracted increasing attention from cell biologists and immunologists. The size of biomolecules and their clusters on cell surface is set at nanoscale, which makes it mandatory to use high and super resolution imaging techniques to realize in situ single molecule imaging of cell membranes. In past decades, some amazing imaging techniques and instruments with super resolution are widely developed for molecule imaging, which can also be further employed for in situ single molecule imaging of cell membranes. With this overview, we attempt to summarize the characteristics of these advanced techniques used for in situ single molecule imaging of cell membranes. We believe that this work will be helpful to promote the technological and methodological developments of super resolution techniques for in situ single molecule imaging of cell membranes and help more researchers better understand which technique is most available for their future exploring of membrane biomolecules, finally promoting the developments of cell biology, immunology and medicine.
    Nanoscale 08/2014; 6(21). DOI:10.1039/C4NR04195J · 6.74 Impact Factor
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    ABSTRACT: A novel electrochemical cytosensor was developed for the fast and high-sensitivity recognition of drug-resistant leukemia K562/ADM cells based on the P-glycoprotein (P-gp) expression level on a cell membrane. The nanocomposite interface of the gold nanoparticles/polyaniline nanofibers (AuNPs/PANI-NF) was chosen to design the biosensor for electrochemical detection. Au/PANI-NF-based cytosensors coated with anti-P-glycoprotein (anti-P-gp) molecules could provide a biomimetic interface for the immunosensing of cell surface P-glycoprotein, and thus could capture the over-expression P-gp cells. Transmission electron microscopy (TEM) indicated that the gold nanoparticles were uniformly anchored along the structure of the PANI-NF surface, displaying fibrillar morphology with a diameter of ∼70 nm, and atomic force microscopy (AFM) further presented the morphology of the nanocomposite film. Owing to the high affinity of anti-P-gp for leukemia K562/ADM cells of the propounded sensing platform, the proposed biosensor exhibited excellent analytical performance for leukemia K562/ADM cells, ranging from 1.6 × 10(2) to 1.6 × 10(6) cells per mL with a detection limit of 80 cells per mL. Recovery experiments indicated that the sensitivity reported here is suitable for practical application. The cell surface P-gp expression level was analysed by flow cytometric experiments, which confirmed the above recognized result. This strategy is also a cost-effective and convenient operation, implying great promise for the sensitive recognition of cancer cells and cell surface receptors; thus, it is helpful in cancer diagnosis.
    The Analyst 06/2014; 139(14). DOI:10.1039/c4an00420e · 3.91 Impact Factor
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    ABSTRACT: endothelial cell proliferation, migration, and tube formation. Vascular endothelial growth factor (VEGF) is considered the most important proangiogenic factor; in particular, VEGF165 plays a critical role in angiogenesis. Here, we evaluated whether gold nanoparticles (AuNPs) could inhibit the VEGF165-induced human umbilical vein endothelial cell (HUVEC) migration and tube formation. AuNPs and VEGF165 were coincubated overnight at 4°C, after which the effects on cell migration and tube formation were assessed. Cell migration was assessed using a modified wound-healing assay and a transwell chamber assay; tube formation was assessed using a capillary-like tube formation assay and a chick chorioallantoic membrane (CAM) assay. We additionally detected the cell surface morphology and ultrastructure using atomic force microscopy (AFM). Furthermore, Akt phosphorylation downstream of VEGFR-2/PI3K in HUVECs was determined in a Western blot analysis. Our study demonstrated that AuNPs significantly inhibited VEGF165-induced HUVEC migration and tube formation by affecting the cell surface ultrastructure, cytoskeleton and might have inhibited angiogenesis via the Akt pathway.
    BioMed Research International 06/2014; 2014:418624. DOI:10.1155/2014/418624 · 2.71 Impact Factor
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    ABSTRACT: A label-free and aggregation-based gold nanorods (AuNRs) probe has been developed for the detection of Pb2+ in aqueous solution, based on the fact that Pb2+ ions induce assembly mediated signal enhancement of cysteine-functionalized AuNRs. Cysteine (Cys) molecules are conjugated on AuNR surfaces to form cysteine-modified AuNRs (Cys-AuNRs), acting as nanoprobes in the detection of Pb2+. Transmission electron microscopy (TEM) and UV–vis absorption spectroscopy data reveal the formation of controlled side-by-side assembly of the AuNRs in the presence of Pb2+. The formation of aggregation of AuNRs significantly enhances detection signals, leading to dramatic decrease in the longitudinal surface plasmon resonance (SPR) absorption. The experiment conditions, including AuNRs aspect ratio, reaction time, pH value and salt concentration, are optimized. The Cys-AuNRs probe is highly sensitive (LOD = 0.1 nM) and selective toward Pb2+ ions, with a liner detection range from 0.1 nM to 1.0 nM. This system only becomes less sensitive when other metal ion is present at a very high concentration (i.e., >0.5 μM). The cost-effective nanoprobes allow rapid and simple determination of the concentration of Pb2+ ions in city tap water samples, with results showing its practicality for the detection of lead in real samples.
    Sensors and Actuators B Chemical 06/2014; 196:252–259. DOI:10.1016/j.snb.2014.01.062 · 4.29 Impact Factor
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    ABSTRACT: Cytochrome c (cyt c) and caspase-9 were critical biomarkers in mitochondria-mediated apoptosis. A novel electrochemical immunosensor was developed for in situ analysis of cyt c and caspase-9 in the cytosol. Gold nanoparticle-polydopamine (AuNP/PDA) composites were used to fabricate the interface of the sensor. The anti-cyt c or anti-caspase-9 functionalized-immunosensor provided a biomimetic interface for immunosensing of cyt c or caspase-9 in Hela cells during apoptosis. The changes in the expression level of cyt c and caspase-9 in the cytosol upon curcumin-induced apoptosis were detected by using the proposed method, and also the influence of different concentrations and incubation times of curcumin-induced Hela cells was investigated. This method achieved a linear range (0.1-100 μM) for standard cyt c and caspase-9, with a detection limit of 0.03 ± 0.01 μM for standard cyt c and 0.08 ± 0.02 μM for standard caspase-9. Moreover, this method was used to detect cells which could detect as low as 100 cells which expressed cyt c and caspase-9, and also the results are in good agreement with standard flow cytometry analysis. The developed electrochemical immunosensor offered a simple and rapid approach for sensitive evaluation of apoptosis markers with considerable specificity and reproducibility, and also the developed strategy could be of great importance in clinical diagnosis and therapeutic research.
    The Analyst 03/2014; DOI:10.1039/c3an02205f · 3.91 Impact Factor
  • Hongxia Zhao, Hua Jin, Jiye Cai
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    ABSTRACT: In this paper, nano-hydroxyapatite/chitosan (n-HA/CS) composites with high compressive strength were prepared by the urease-catalyzed method (UCM) under mild conditions. The samples were characterized by infrared spectroscopy (IR), X-ray diffraction (XRD), laser diffraction particle size analyzer (LDPSA) and transmission electron microscopy (TEM). The results showed that the shape, size and crystallinity index of nano-HA crystals in composites were similar to that of nature bone. The n-HA/CS samples prepared by UCM had smaller particle size compared with those prepared by traditional co-precipitation technique. Moreover, mechanical testing showed that the maximum compressive strength of the composite materials was 130.3 +/- 9.3 MPa, which was close to that of cortical bone. UCM has great potential in fabricating non-sintering and drug-loaded bioceramics with improved mechanical features in bone tissue engineering.
    Materials Letters 02/2014; 116:293-295. DOI:10.1016/j.matlet.2013.05.082 · 2.27 Impact Factor
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    ABSTRACT: Multidrug resistance (MDR) mediated by ATP-binding cassette (ABC) transporters through efflux of antineoplastic agents from cancer cells is a major obstacle to successful cancer chemotherapy. The inhibition of these ABC transporters is thus a logical approach to circumvent MDR. There has been intensive research effort to design and develop novel inhibitors for the ABC transporters to achieve this goal. In the present study, we evaluated the ability of UMMS-4 to modulate P-glycoprotein (P-gp/ABCB1)-, breast cancer resistance protein (BCRP/ABCG2)- and multidrug resistance protein (MRP1/ABCC1)-mediated MDR in cancer cells. Our findings showed that UMMS-4, at non-cytotoxic concentrations, apparently circumvents resistance to ABCB1 substrate anticancer drugs in ABCB1-overexpressing cells. When used at a concentration of 20 μmol/L, UMMS-4 produced a 17.53-fold reversal of MDR, but showed no effect on the sensitivity of drug-sensitive parental cells. UMMS-4, however, did not significantly alter the sensitivity of non-ABCB1 substrates in all cells and was unable to reverse ABCG2- and ABCC1-mediated MDR. Additionally, UMMS-4 profoundly inhibited the transport of rhodamine 123 (Rho 123) and doxorubicin (Dox) by the ABCB1 transporter. Furthermore, UMMS-4 did not alter the expression of ABCB1 at the mRNA and protein levels. In addition, the results of ATPase assays showed that UMMS-4 stimulated the ATPase activity of ABCB1. Taken together, we conclude that UMMS-4 antagonizes ABCB1-mediated MDR in cancer cells through direct inhibition of the drug efflux function of ABCB1. These findings may be useful for the development of safer and more effective MDR modulator.
    American Journal of Cancer Research 01/2014; 4(2):148-60. · 3.97 Impact Factor
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    ABSTRACT: A sensitive and facile electrochemical biosensor has been developed for monitoring the protein nitration damage affected by the nitro free radicals(NO2). The NO2 radicals is generated from hemin-catalyzed oxidation of nitrite (NO(-)2) in the presence of hydrogen peroxide (H2O2). In this work, nanocomposite films of graphene-gold nanoparticles (EG-AuNPs) were modified on the glassy carbon electrode by electropolymerization. Bovine serum albumin (BSA) was then further assembled on EG-AuNPs film through Au-S bond. The damage of BSA molecule was caused by the NO2 radicals which was generated from the NaNO2/hemin/H2O2 nitration reagent. The differential pulse voltammetry was used to detect the damage of BSA molecule. Fluorescence spectra and circular dichroism spectrum further confirmed the nitration damage of BSA. Moreover, the lowest concentration at which the BSA damage was detected is 28.9µM NO(-)2 or H2O2, and the volume ratio of NO(-)2 and H2O2 was 1:1 in the hemin/NO(-)2/H2O2 nitration reagent. The results demonstrated that the proposed electrochemical method can be used to detect protein damage affected by nitration reagent. The developed electrochemical biosensor is envisioned to have promising applications in protein damage studies.
    Biosensors & Bioelectronics 11/2013; 54C:628-633. DOI:10.1016/j.bios.2013.11.052 · 6.45 Impact Factor
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    ABSTRACT: The inhibition of the binding between VEGFs and their receptors reduces angiogenesis and retards tumor growth. Owing to the large amount of antibodies required, the antibody-based anti-angiogenic drug remains limited. Gold nanoparticles (AuNPs) displayed excellent biocompatibility, low toxicity and anti-angiogenic effect, but the mechanism of anti-angiogenesis was unknown. Here, the antitumor effects of a well-dispersed AuNPs, specifically regarding its influence on VEGF signaling, were examined mechanistically. The effects of AuNPs on the interaction of VEGF with its receptor, VEGFR2 were observed using near-field scanning optical microscopy/quantum dot (NSOM/QD) imaging. We found AuNPs can reduce VEGF165-induced VEGFR2 and AKT phosphorylation. Furthermore, the antitumor effects of AuNPs were determined using xenograft and ascites model. AuNPs inhibited VEGF165-VEGFR2 interaction and suppressed the formation of nanodomains of VEGFR2 on the HUVEC. As determined by CD34 immunhistochemistry, AuNPs reduced angiogenesis in a liver tumor nude mice model, as observed by a decreased microvascular density in liver tumor sections and reduced the tumor weight and volume. In addition, AuNPs inhibited ascites formation in mice. Taken together, this study provides new insights into nanomaterial-based antitumor drug development.
    Journal of Biomedical Nanotechnology 10/2013; 9(10):1746-56. DOI:10.1166/jbn.2013.1678 · 7.58 Impact Factor

Publication Stats

737 Citations
298.86 Total Impact Points

Institutions

  • 2004–2015
    • Jinan University (Guangzhou, China)
      • College of Science and Engineering
      Shengcheng, Guangdong, China
    • South China Normal University
      Shengcheng, Guangdong, China
  • 2014
    • Macau University of Science and Technology
      Macao, Macau, Macao
  • 2004–2014
    • University of Jinan (Jinan, China)
      Chi-nan-shih, Shandong Sheng, China
  • 2005–2009
    • University of Illinois at Chicago
      • Department of Microbiology and Immunology (Chicago)
      Chicago, IL, United States
  • 2007
    • Guangzhou University
      Shengcheng, Guangdong, China