Fang Pu

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (92)326.58 Total impact

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    ABSTRACT: Previous studies have focused mainly on individual factors affecting the balance function of older adults. However, it is largely unknown whether the balance functions of older adults are affected by multiple factors occurring simultaneously, and what is predominant among these factors.
    Aging clinical and experimental research. 09/2014;
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    ABSTRACT: Previous studies have suggested that amnestic mild cognitive impairment (aMCI) is associated with changes in cortical morphological features, such as cortical thickness, sulcal depth, surface area, gray matter volume, metric distortion, and mean curvature. These features have been proven to have specific neuropathological and genetic underpinnings. However, most studies primarily focused on mass-univariate methods, and cortical features were generally explored in isolation. Here, we used a multivariate method to characterize the complex and subtle structural changing pattern of cortical anatomy in 24 aMCI human participants and 26 normal human controls. Six cortical features were extracted for each participant, and the spatial patterns of brain abnormities in aMCI were identified by high classification weights using a support vector machine method. The classification accuracy in discriminating the two groups was 76% in the left hemisphere and 80% in the right hemisphere when all six cortical features were used. Regions showing high weights were subtle, spatially complex, and predominately located in the left medial temporal lobe and the supramarginal and right inferior parietal lobes. In addition, we also found that the six morphological features had different contributions in discriminating the two groups even for the same region. Our results indicated that the neuroanatomical patterns that discriminated individuals with aMCI from controls were truly multidimensional and had different effects on the morphological features. Furthermore, the regions identified by our method could potentially be useful for clinical diagnosis.
    The Journal of neuroscience : the official journal of the Society for Neuroscience. 08/2014; 34(32):10541-53.
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    ABSTRACT: Logic gates can convert input signals into a defined output signal, which is the fundamental basis of computing. Inspired by molecular switching from one state to another under an external stimulus, molecular logic gates are explored extensively and recognized as an alternative to traditional silicon-based computing. Among various building blocks of molecular logic gates, nucleic acid attracts special attention owing to its specific recognition abilities and structural features. Functional materials with unique physical and chemical properties offer significant advantages and are used in many fields. The integration of nucleic acids and functional materials is expected to bring about several new phenomena. In this Progress Report, recent progress in the construction of logic gates by combining the properties of a range of smart materials with nucleic acids is introduced. According to the structural characteristics and composition, functional materials are categorized into three classes: polymers, noble-metal nanomaterials, and inorganic nanomaterials. Furthermore, the unsolved problems and future challenges in the construction of logic gates are discussed. It is hoped that broader interests in introducing new smart materials into the field are inspired and tangible applications for these constructs are found.
    Advanced Materials 07/2014; · 14.83 Impact Factor
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    ABSTRACT: Molecular logic gates in response to chemical, biological or optical input signals at a molecular level have received much interest over the past decade. Herein, we construct "plug and play" logic systems based on the fluorescence switching of guest molecules confined in coordination polymer nanoparticles generated from nucleotide and lanthanide ions. In the system, the addition of new modules directly enables new logic functions. PASS 0, YES, PASS 1, NOT, IMP, OR, and AND gates are successfully constructed in sequence. Moreover, different logic gates (AND, INH, and IMP) can be constructed using different guest molecules and the same input combinations. The work will be beneficial to the future logic design and expand the applications of coordination polymers.
    ACS Applied Materials & Interfaces 05/2014; · 5.01 Impact Factor
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    ABSTRACT: Artificial light-harvesting antenna materials as potential mimics for photosynthetic systems have attracted intense attention recently. Herein, a new modular approach to construct light-harvesting material, which involves the self-assembly of coordination polymer nanoparticles (CPNs) at room temperature, is presented. Fluorescence resonance energy transfer (FRET) occurs between donor and acceptor molecules encapsulated in the CPNs, and the emission signal of acceptor is amplified significantly. To the best of our knowledge, this is the first example of artificial light-harvesting material generated from biomolecule-based coordination polymer nanoparticles. The modularity of the material makes it convenient to manipulate the system by changing the composite of CPNs and the type and amount of dyes confined, implying it is a general strategy. The material functions not only in fluid medium, but also in the form of solid state, which extends its application areas greatly. Furthermore, photocurrent generation can be realized by the dye-encapsulated CPNs system upon irradiation with visible light, implying the potential usefulness in light-energy conversion and photoelectronic applications. Besides, the creation of FRET system provides a platform to mimic dual-channel logic gate at nanoscale level, which is beneficial to the construction of integrated logic devices with multiple functions.
    Advanced Functional Materials 04/2014; · 10.44 Impact Factor
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    ABSTRACT: This study presented optimal estimation of total plantar force with a suitable sensor layout and a reliable equation for monitoring gait in daily life activities. The plantar pressure of 10 subjects during level walking was measured by Pedar® insoles at 100 Hz for establishing models and selecting the optimal one. Four types of virtual sensors with different sizes were designed. Stepwise linear regressions were performed to reconstruct total plantar force based on each particular type of virtual sensor. 14 models were established, which met the requirements of the explained variance of the regression model and the multicollinearity of the predictors. Estimated total plantar force from each model was compared with the real data from the Pedar® insoles. According to the correlation coefficient (R) and the root mean square error divided by the peak force (RMSE/PF), the optimal model had three sensors located under the heel and metatarsal. Another four subjects were used for validating the optimal model by performing level walking, running, vertical jump-landing, stair ascending and descending. For these five common activities, the correlation was high (R > 0.970) and the error was low (RMSE/PF < 10%). Therefore, this model can accurately estimate total plantar force in daily life activities.
    Journal of Mechanics in Medicine and Biology 03/2014; 14(03). · 0.76 Impact Factor
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    ABSTRACT: The aim of this study was to develop a feedback system to assist gait rehabilitation of cerebral palsy (CP) toe walkers with dynamic equinus. Plantar pressure of the forefoot and the heel was collected by sensorized insoles embedded in custom-built shoes and transmitted to a smartphone via Bluetooth. Dynamic foot pressure index of each stride was calculated by purpose-designed software running in the smartphone to distinguish toe-walking strides from normal strides in real time. An auditory signal would be produced to alert the patient each time a toe-walking stride was detected. For CP toe walkers, the one-way analysis of variance indicated a significant difference (F1,14 = 19.492, P = 0.001) in dynamic foot pressure index between the affected side (31.4 ± 12.0) and the unaffected side (58.6 ± 2.5). In addition, the validation test showed that this system can distinguish toe-walking strides from normal strides of children with CP with an accuracy of 95.3%. This system was able to monitor the toe-walking strides of children with CP in real time and had the potential to enhance rehabilitation training efficiency and correct toe-walking gait in children with CP with dynamic equinus.
    American journal of physical medicine & rehabilitation / Association of Academic Physiatrists 02/2014; 93(2):122-9. · 1.56 Impact Factor
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    ABSTRACT: Upconversion applied in logic gate: The Upconversion nanoparticles (UCNPs) give multi emissions when excited by midrange IR radiation. A ternary logic system is designed which combines the unique characters of UCNPs with the ensemble of DNA. By regulating the emission of UCNPs, this logic system involvs more than two states, and the density of information can be increased.
    Small 01/2014; · 7.82 Impact Factor
  • Yan Li, Xue Rui, Shuyu Li, Fang Pu
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    ABSTRACT: Background Graph theoretical analysis has recently become a popular research tool in neuroscience, however, there have been very few studies on brain responses to music perception, especially when culturally different styles of music are involved. Methods Electroencephalograms were recorded from ten subjects listening to Chinese traditional music, light music and western classical music. For event related potentials, phase coherence was calculated in the alpha band and then constructed into correlation matrices. Clustering coefficients and characteristic path lengths were evaluated for global properties, while clustering coefficients and efficiency were assessed for local network properties. Results Perception of light music and western classical music manifested small-world network properties, especially with a relatively low proportion of weights of correlation matrices. For local analysis, efficiency was more discernible than clustering coefficient. Nevertheless, there was no significant discrimination between Chinese traditional and western classical music perception. Conclusions Perception of different styles of music introduces different network properties, both globally and locally. Research into both global and local network properties has been carried out in other areas; however, this is a preliminary investigation aimed at suggesting a possible new approach to brain network properties in music perception.
    Computers in Biology and Medicine. 01/2014;
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    ABSTRACT: Fluorescence switching of guest molecules confined in coordination polymer nanoparticles (CPNs) generated from nucleotides and lanthanide ions are used to construct multiconfigurable logic gates. Moreover, the potential of the material as fluorescent probe with large Stokes shift is demonstrated for cellular imaging. In this work the logic gate is integrated into the therapeutic agent and this will be highly beneficial in future molecular computing.
    Advanced Materials 11/2013; · 14.83 Impact Factor
  • Fang Pu, Jinsong Ren
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    ABSTRACT: Fluorescent silica nanotubes are attracting increasing interest because of their versatile applicability in a range of diverse fields. By using sol-gel transcription of tetraethylorthosilicate (TEOS) from various soft templates, silica nanotubes can be conveniently prepared. Metal-peptide hybrids with well-defined nanostructures and outstanding functionalities are very interesting candidates to serve as templates. Herein, we demonstrate that glutathione (GSH) can act as a building block for a bioinspired structure with dimensions down to the nanoscale, based on specific interactions between metal ions and the peptide. Congo red is able to selectively stain the nanofibres obtained, and appears apple-green in colour, implying that Congo red is promising to serve as an effective and convenient probe for determining the self-assembly of GSH and copper ions. Furthermore, silica nanotubes are synthesized using the nanofibres as a template in a very simple way. The silica nanotubes can be lit up by biomolecule-templated metal nanoparticles or nanoclusters and emit bright fluorescence. This work will certainly open up new opportunities in fabricating a broad range of nanostructured materials with versatile functionalities.
    Nanotechnology 09/2013; 24(37):375603. · 3.84 Impact Factor
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    ABSTRACT: Fluorescent conjugated polyelectrolytes (CPEs) with a large extinction coefficient, high quantum efficiency, good photostability, and efficient intramolecular/intermolecular exciton migration have recently received great research attention as a new generation of fluorescent probes. Herein, the unique properties of fluorescent conjugated polyelectrolyte and mesoporous silica nanoparticles (MSNs) are combined to prepare a pH-sensitive drug delivery and cellular imaging system using a simple and more general method. To the best of our knowledge, this is the first proof-of-concept investigation on the use of fluorescent conjugated polyelectrolyte as both the capping agent of MSNs and the cellular imaging probe in parallel. Moreover, the bifunctional nanomaterial exhibits low cytotoxicity and good biocompatibility demonstrated by a cytotoxicity assay against HepG2 cells and a hemolysis assay against human red-blood cells. It is a promising candidate for simultaneous diagnostics and therapeutics and can also provide a general strategy to develop novel multifunctional nanomedical systems.
    ChemPlusChem 07/2013; 78(7).
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    ABSTRACT: Hydrophobicity has been an obstacle that hinders the use of many anticancer drugs. A critical challenge for cancer therapy concerns the limited availability of effective biocompatible delivery systems for most hydrophobic therapeutic anticancer drugs. In this study, we have developed a targeted near-infrared (NIR)-regulated hydrophobic drug-delivery platform based on gold nanorods incorporated within a mesoporous silica framework (AuMPs). Upon application of NIR light, the photothermal effect of the gold nanorods leads to a rapid rise in the local temperature, thus resulting in the release of the entrapped drug molecules. By integrating chemotherapy and photothermotherapy into one system, we have studied the therapeutic effects of camptothecin-loaded AuMP-polyethylene glycol-folic acid nanocarrier. Results revealed a synergistic effect in vitro and in vivo, which would make it possible to enhance the therapeutic effect of hydrophobic drugs and decrease drug side effects. Studies have shown the feasibility of using this nanocarrier as a targeted and noninvasive remote-controlled hydrophobic drug-delivery system with high spatial/temperal resolution. Owing to these advantages, we envision that this NIR-controlled, targeted drug-delivery method would promote the development of high-performance hydrophobic anticancer drug-delivery system in future clinical applications.
    Chemistry 06/2013; · 5.93 Impact Factor
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    ABSTRACT: BACKGROUND AND PURPOSE: Automated anatomical labeling (AAL) provides an automatic brain region segmentation method to allow objective measurement of regional brain volume. Nonlinear registration plays a critical role in such automated region-based volumetry. The aim of this study was to compare age-related brain regional volume changes using two nonlinear registration methods in statistical parametric mapping (SPM). MATERIAL AND METHODS: The study included 176 right-handed healthy participants (age range: 18-94years). A total of 90 brain regions for each subject were automatically extracted, based on the AAL atlas, and two nonlinear registration methods (Normalization and DARTEL Toolbox in SPM5) were applied. Three-way ANOVA was performed to estimate the effects of brain region, each registration method and each hemisphere on regional volumes. Age-related brain-volume changes were also investigated by linear regression analysis for each nonlinear registration method. RESULTS: Significant differences were found in volume among different brain regions (P<0.001) with the two nonlinear registration methods (P=0.011). Volumes of the corresponding brain region were significantly different (P=0.037) between two hemispheres, and age-related volume reductions were unevenly distributed across regions. The most dramatic decreases in volume were found in the bilateral insula, middle frontal regions and cingulum. Rankings of the decreased brain regional volumes differed between the two registration techniques and adjustment methods. CONCLUSION: The inferred age-related volume atrophy patterns based on the AAL atlas were largely dependent on the choice of registration methodology.
    Journal of Neuroradiology 04/2013; · 1.24 Impact Factor
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    ABSTRACT: Novel nanoparticulate contrast agents with low systemic toxicity and inexpensive character have exhibited more advantages over routinely used small molecular contrast agents for the diagnosis and prognosis of disease. Herein, we designed and synthesized PEGylated hybrid ytterbia nanoparticles as high-performance nanoprobes for X-ray computed tomography (CT) imaging and magnetic resonance (MR) imaging both in vitro and in vivo. These well-defined nanoparticles were facile to prepare and cost-effective, meeting the criteria as a biomedical material. Compared with routinely used Iobitridol in clinic, our PEG-Yb2O3:Gd nanoparticles could provide much significantly enhanced contrast upon various clinical voltages ranging from 80 kVp to 140 kVp owing to the high atomic number and well-positioned K-edge energy of ytterbium. By the doping of gadolinium, our nanoparticulate contrast agent could perform perfect MR imaging simultaneously, revealing similar organ enrichment and bio-distribution with the CT imaging results. The super improvement in imaging efficiency was mainly attributed to the high content of Yb and Gd in a single nanoparticle, thus making these nanoparticles suitable for dual-modal diagnostic imaging with a low single-injection dose. In addition, detailed toxicological study in vitro and in vivo indicated that uniformly sized PEG-Yb2O3:Gd nanoparticles possessed excellent biocompatibility and revealed overall safety.
    Nanoscale 04/2013; · 6.73 Impact Factor
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    ABSTRACT: We fabricated a keypad lock system based on mesoporous silica nanoparticle (MSN)-nucleic acid conjugates. DNA strand displacement triggered the release of guest molecules entrapped within the pores of MSNs through logic-based control.
    Chemical Communications 02/2013; · 6.38 Impact Factor
  • Medical Engineering & Physics 01/2013; · 1.78 Impact Factor
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    ABSTRACT: We demonstrate a novel, rapid and label-free assay for the detection of Ag(+) and biothiols with high sensitivity and selectivity by utilization of Ag nanoparticle-decorated graphene quantum dots.
    Chemical Communications 01/2013; · 6.38 Impact Factor
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    ABSTRACT: The ankle-brachial index (ABI), defined as the ratio of systolic pressure in the ankle arteries and that in the brachial artery, was a useful noninvasive method to detect arterial stenoses. There had been a lot of researches about clinical regularities of ABI; however, mechanism studies were less addressed. For the purpose of a better understanding of the correlation between vascular stenoses and ABI, a computational model for simulating blood pressure and flow propagation in various arterial stenosis circumstances was developed with a detailed compartmental description of the heart and main arteries. Particular attention was paid to the analysis of effects of vascular stenoses in different large-sized arteries on ABI in theory. Moreover, the variation of ABI during the increase of the stenosis severity was also studied. Results showed that stenoses in lower limb arteries, as well as, brachial artery, caused different variations of blood pressure in ankle and brachial arteries, resulting in a significant change of ABI. Furthermore, the variation of ABI became faster when the severity of the stenosis increased, validating that ABI was more sensitive to severe stenoses than to mild/moderate ones. All these in findings revealed the reason why ABI was an effective index for detecting stenoses, especially in lower limb arteries.
    The Scientific World Journal 01/2013; 2013:185691. · 1.73 Impact Factor
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    ABSTRACT: Previous neuroimaging studies have suggested that cerebral changes over normal aging are not simply characterized by regional alterations, but rather by the reorganization of cortical connectivity patterns. The investigation of structural covariance networks (SCNs) using voxel-based morphometry is an advanced approach to examining the pattern of covariance in gray matter (GM) volumes among different regions of the human cortex. To date, how the organization of critical SCNs change during normal aging remains largely unknown. In this study, we used an SCN mapping approach to investigate eight large-scale networks in 240 healthy participants aged 18-89 years. These participants were subdivided into young (18-23 years), middle aged (30-58 years), and older (61-89 years) subjects. Eight seed regions were chosen from widely reported functional intrinsic connectivity networks. The voxels showing significant positive associations with these seed regions were used to describe the topological organization of an SCN. All of these networks exhibited non-linear patterns in their spatial extent that were associated with normal aging. These networks, except the primary motor network, had a distributed topology in young participants, a sharply localized topology in middle aged participants, and were relatively stable in older participants. The structural covariance derived using the primary motor cortex was limited to the ipsilateral motor regions in the young and older participants, but included contralateral homologous regions in the middle aged participants. In addition, there were significant between-group differences in the structural networks associated with language-related speech and semantics processing, executive control, and the default-mode network (DMN). Taken together, the results of this study demonstrate age-related changes in the topological organization of SCNs, and provide insights into normal aging of the human brain.
    Frontiers in Human Neuroscience 01/2013; 7:98. · 2.91 Impact Factor

Publication Stats

430 Citations
326.58 Total Impact Points

Institutions

  • 2009–2014
    • Chinese Academy of Sciences
      • Laboratory of Chemical Biology
      Peping, Beijing, China
    • Beijing University of Aeronautics and Astronautics (Beihang University)
      • • School of Biological and Medical Engineering
      • • State Key Laboratory for Virtual Reality Technology and Systems
      Peping, Beijing, China
  • 2009–2013
    • Northeast Institute of Geography and Agroecology
      • Laboratory of Chemical Biology
      Peping, Beijing, China
  • 2004–2005
    • Shanxi Medical University
      • Department of Cardiothoracic Surgery
      Yangkü, Shanxi Sheng, China