A. SCULEAN

University of Münster, Muenster, North Rhine-Westphalia, Germany

Are you A. SCULEAN?

Claim your profile

Publications (96)106.62 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: The primary goal in cause-related periodontitis treatment is to remove hard and soft bacterial deposits which should result in a smooth and biocompatible root surface. In addition, repeated instrumentation may lead to loss of tooth substance and thus, it should be avoided. The purpose of this in-vitro-study was to evaluate surface roughness and substance loss after four different treatment modalities. Methods: Multi-species biofilms have been formed on standardized dentine specimens for 3.5 d. Thereafter, the dentine specimens were placed into artificial pockets and different treatments (air-polishing AIR-Flow Master with PERIO-FLOW System using erythritol (APE), air-polishing using erythritol combined with chlorhexidine digluconate (APE-CHX), hand instrumentation by using curettes (CUR) and ultrasonication (US)) were used. Biofilm formation and treatments were repeated four times. Before and after one (1×) and five (5×) treatments, surface roughness (Ra and Rz) and the thickness of the dentine specimens were measured. Statistical analysis was made by using ANOVA with Post-Hoc LSD. Results: Substance loss (loss of thickness) was the highest when applying CUR (20±21 µm after 1×, 128±40 µm after 5×), all other treatments caused only a loss of thickness up to 8 µm (1×) and 14 µm (5×). The difference of CUR to the others treatments was each significant (p<0.01 after 1×, p<0.001 after 5×). After 5× the surface roughness was higher for CUR (Ra, Rz) and for US (Rz) than for the untreated control (each p< 0.05), no difference was found for APE and APE-CHX in comparison with the control. Conclusion: Mechanical debridement by using APE and APE-CHX results in smooth surfaces and prevents substance loss. The study was funded by EMS, Nyon, Switzerland.
    2014 IADR/PER Congress; 09/2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: The primary goal in cause-related periodontitis treatment is to remove hard and soft bacterial deposits which should result in a surface preventing recolonisation. The purpose of this in-vitro-study was to evaluate removal and reformation of a multi-species biofilm after four different treatment modalities. Method: Multi-species biofilms consisting of 12 species being associated with periodontitis have been formed on standardized dentine specimens for 3.5 d. Subsequently, the dentine specimens were placed into artificial pockets and different treatments (air-polishing AIR-Flow Master with PERIO-FLOW System using erythritol (APE), air-polishing using erythritol combined with chlorhexidine digluconate (APE-CHX), hand instrumentation by using curettes (CUR) and ultrasonication (US)) were applied. Biofilm formation and treatment were repeated four times. After one (1×) and five (5×) treatments, the counts (log10) of the remaining bacteria as well as those in the recolonized biofilm (total numbers as well as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) were determined. Statistical analysis was made by using ANOVA with Post-Hoc LSD. Result: 1× and 5× treatment by CUR reduced the total bacterial counts by 2 log10 cfu (each p<0.01), APE and US by about 3 log10 and APE-CHX by 4 log10 cfu (each p<0.001). Recolonization did not show any difference for the total counts after 1× treatment, after 5× treatment total counts were less after US and APE (each p<0.01) and after APE-CHX (p<0.001). A. actinomycetemcomitans recolonized less in the biofilm after 1× APE-CHX. After 5×treatment, the recolonization of T. forsythia and T. denticola in biofilm was decreased after all treatments, those of all four in more detail determined pathogens was reduced but not completely prevented after APE-CHX. Conclusion: The cleaning efficacy is more pronounced when using air-polishing or US. The addition of CHX to APE acts bactericidal and inhibits reformation of biofilm.
    2014 IADR/PER Congress; 09/2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate early wound healing, tooth staining and patient acceptance of two different post-surgical maintenance protocols. Method: After a surgical intervention (implant placement or periodontal surgery), 40 patients were randomly included in two groups and were instructed in two different post-surgical maintenance protocols. Patients were assigned to 2 weeks rinsing with 0.05% CHX/herbal extract combination (test) or 0.1% CHX solution (control). Early-wound healing was evaluated clinically and immunologically. Staining effects and patient acceptance were evaluated using VAS questionnaire. Result: 63% of all surgical interventions consisted of an open-flap debridement while in the rest of 37% an dental implant was placed. Both groups showed comparable wound-healing profiles. No statistically significant differences could be observed in terms of the Early-Wound-Healing and Plaque Indices between the 2 groups. Plaque regrowth was comparable in the 2 groups (p=0.4123). No statistically significant changes between week 1 and week 2 were found in terms of tooth staining in the 2 groups at both visits (p=0.1668) and between week 1 and week 2 (test: p=0.5675; control: p=0.2458). In the test group statistical significant more patients reported tooth stain (p>0.0001) and have been annoyed by this (p>0.0001). Patients in control group documented irritation of taste reaching trend to statistical significance at week 1. However, in week 2 the results were statistically significant (p=0.0042). Conclusion: Both chlorhexidine supplements showed equal healing properties and inhibition of plaque formation. Tooth stain occurred with both mouthwashes but there was a trend towards statistical significance with the lower CHX concentration/herbal extract. Patients were annoyed by increased staining and taste irritation occurred more frequently in the group using higher CHX concentrations.
    2014 IADR/PER Congress; 09/2014
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate salivary and microbial biomarkers in gingivitis patients using a powered toothbrush/irrigator combination or a manual toothbrush over a period of 8 weeks. Method: This was a single-center, randomized, parallel, examiner-blind, 8-week, controlled clinical trial. 60 adult subjects with evidence of dental plaque and gingivitis (minimum of 15 bleeding on probing positive sites) were randomly assigned to either use a powered toothbrush/irrigator combination (Oral-B® Professional Care Oxyjet 1000 Center, Test) or a regular manual toothbrush (Oral-B® Indicator 35, Control). Subjects were stratified at baseline according to age, smoking status, gingivitis-, and plaque-index to balance and randomly assign the treatment groups. Subjects from both groups used standard fluoridated toothpaste (Blend-a-Med Classic). In both groups, tooth brushing was performed twice daily at home following manufacturer's instructions and no interdental cleaning aids had to be used. At Baseline and at Week-2, Week-4 and Week-8, whole saliva was collected by passive drawling while gingival crevicular fluid (GCF) and dental plaque were collected at the mesiobuccal sites of teeth 16 and 17. Oral fluid biomarkers included levels of Interleukin1-beta (IL1-beta), Matrix Metalloproteinase-3 (MMP-3), and MMP-8, while microbial data included Campylobacter rectus, Capnocytophaga sp., Eikenella corrodens, Eubacterium nodatum, and Parvimonas micra. Between-groups comparisons were performed using analysis of covariance with baseline as covariate. Result: No between treatment differences were statistically significant for both oral fluid and pathogen biomarker at any visit with the exception of IL1-beta at Week-8, where the Test group exhibited significantly (p=0.0313) lower mean levels of IL1-beta in GCF than Control. Conclusion: The use of a powered toothbrush/irrigator combination resulted in lower levels of gingival inflammation as indicated by a lower level of IL1-beta in GCF at Week-8 when compared to the use of a regular manual toothbrush.
    2014 IADR/PER Congress; 09/2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate clinically the long-term results following treatment of deep intrabony defects with a combination of Platelet Rich Plasma (PRP) + a natural bone mineral (NBM) + a bioresorbable collagen membrane (GTR) to NBM + GTR. Method: Sixteen patients suffering from advanced chronic periodontitis, and each of whom displayed one advanced intrabony defect were randomly treated with either PRP+NBM+GTR (test) or NBM+GTR (control). Clinical parameters were evaluated at baseline, at 1 year and 7 years after treatment. The primary outcome variable was clinical attachment level (CAL). Results: The test sites showed a reduction in mean probing depth (PD) from 8.5±1.4 mm to 3.0 ± 0.5 mm (p<0.001) at 1 year and to 3.6 ± 0.7 mm (p<0.001) at 7 years, respectively. In the control group mean PD was reduced from 8.5 ± 1.7 mm to 3.1 ± 1.1 mm at 1 year (p<0.00) and to 3.4 ± 1.1 mm at 7 years. In the test group mean CAL changed from 10.4 ± 1.9 mm to 5.5 ± 0.9 mm at 1 year (p<0.001) and to 5.8 ± 1.4 mm at 7 years, respectively. In the control group mean CAL changed from 10.3 ± 2.2 mm to 5.4 ± 1.5 mm at 1 year (p<0.001) and to 6.3 ± 2.0 mm at seven years. No statistically significant differences in any of the investigated parameters were observed between the two groups at 1 and 7 years. Conclusion: Both treatments resulted in significant PPD reductions and CAL gains. The present results have shown that the clinical improvements obtained with both treatments can over a period of 7 years.
    IADR General Session and Exhibition 2014; 06/2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Several studies focus on a relationship between periodontitis (PA) and rheumatoid arthritis (RA). Citrullination of proteins and peptides by human peptidylarginine-deiminases (PAD) followed by generation of antibodies is an important factor in RA. Recently a PAD has been purified from Porphyromonas gingivalis (PPAD) being unique in bacteria. The aim of this case-control study was to determine activities of PAD and PPAD in periodontium and serum antibody levels against citrullinated epitopes of PPAD in RA patients. Method: Gingival crevicular washes of 52 RA patients (48 with PA (RA-PA) and 4 without (RA-NoPA)) and 44 individuals without RA (NoRA, 28 with PA (NoRA-PA) and 16 without PA (NoRA-NoPA)) have been evaluated for activities of PAD and PPAD and for presence of Porphyromonas gingivalis. Serum has been analyzed for antibodies against citrullinated epitopes of PPAD. Result: PAD activity has been detected in 25 (48%) of RA patients (24 RA-PA) and in 24 (55%) of NoRA (19 NoRA-PA). PPAD activity has been measured in 30 (58%) of RA-patients (29 RA-PA) and in 21 (50%) of No-RA-patients (19 NoRA-PA). Enzyme activities were significantly higher in NoRA-PA compared with NoRA-NoPA (p=0.022; p=0.004). PPAD activity correlated neither with PAD activity nor with counts of P. gingivalis. In contrast PPAD activity has also been detected in 16 out of 38 P. gingivalis negative samples. PPAD positive but P. gingivalis negative samples showed high antibody levels against citrullinated epitopes of PPAD. Conclusion: Peptidylarginine-deiminases are active within the periodontium and can citrullinate proteins and peptides. P. gingivalis seems to be able to release PPAD acting in sites not colonized by the species. The citrullination by human PAD and PPAD may generate antibodies after breaking immunotolerance in susceptible individuals.
    IADR General Session and Exhibition 2014; 06/2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Connective tissue grafts are frequently applied together with Emdogain® for root coverage. It is, however, unknown whether fibroblasts from the gingiva and from the palate respond similarly to Emdogain®. To evaluate the effect of Emdogain® on fibroblast from palatal and gingival connective tissue by using a genome-wide microarray approach. Methods: Human palatal and gingival fibroblasts were exposed to Emdogain® and RNA subjected to microarray analysis followed by gene ontology screening with DAVID functional annotation clustering, KEGG pathway analysis, and the STRING functional protein association network. Microarray results were confirmed by qRT-PCR analysis. Results: Transcription levels of 106 genes were at least 5-fold changed in both, gingival and palatal fibroblasts upon exposure to Emdogain®. Gene Ontology screening assigned the respective genes into 118 biological processes, six cellular components, eight molecular functions, and five pathways. Among the striking pattern was the changing expression of ligands targeting the TGF-β and gp130 receptor family, as well as the mesenchymal epithelial transition. Moreover, Emdogain® caused expression changes of receptors for chemokines, lipids and hormones, and transcription factors such as smad-3, PPARγ and ETS. Conclusion: The present data suggest that Emdogain® causes substantial alterations in gene expression with a similar pattern in palatal and gingival fibroblasts.
    IADR General Session and Exhibition 2014; 06/2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: To objectively determine the difference in colour between the peri-implant soft tissue at titanium and zirconia abutments.
    Clinical Oral Implants Research 06/2014; · 3.43 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background and Objective Connective tissue grafts are frequently applied, together with Emdogain®, for root coverage. However, it is unknown whether fibroblasts from the gingiva and from the palate respond similarly to Emdogain. The aim of this study was therefore to evaluate the effect of Emdogain® on fibroblasts from palatal and gingival connective tissue using a genome-wide microarray approach.Material and Methods Human palatal and gingival fibroblasts were exposed to Emdogain® and RNA was subjected to microarray analysis followed by gene ontology screening with Database for Annotation, Visualization and Integrated Discovery functional annotation clustering, Kyoto Encyclopedia of Genes and Genomes pathway analysis and the Search Tool for the Retrieval of Interacting Genes/Proteins functional protein association network. Microarray results were confirmed by quantitative RT-PCR analysis.ResultsThe transcription levels of 106 genes were up-/down-regulated by at least five-fold in both gingival and palatal fibroblasts upon exposure to Emdogain®. Gene ontology screening assigned the respective genes into 118 biological processes, six cellular components, eight molecular functions and five pathways. Among the striking patterns observed were the changing expression of ligands targeting the transforming growth factor-beta and gp130 receptor family as well as the transition of mesenchymal epithelial cells. Moreover, Emdogain® caused changes in expression of receptors for chemokines, lipids and hormones, and for transcription factors such as SMAD3, peroxisome proliferator-activated receptor gamma and those of the ETS family.Conclusion The present data suggest that Emdogain® causes substantial alterations in gene expression, with similar patterns observed in palatal and gingival fibroblasts.
    Journal of Periodontal Research 05/2014; · 1.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: The effect of moxifloxacin was compared with ofloxacin and doxycycline against bacteria associated with periodontitis within a biofilm (single strain and multi-species population) in vitro. Method: Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of moxifloxacin, ofloxacin and doxycyline were determined against single strains and multi-species populations in planktonic state. Single-species biofilms of two Porphyromonas gingivalis and two Aggregatibacter actinomycetemcomitans strains and a multi-species biofilm consisting of 12 species were formed for 3 days. The minimal biofilm eradication concentrations (MBECs) were determined after exposing the biofilms to the antibiotics (0.002 – 512 mg/l) for 18 h, addition of nutrient broth for 3 d and subsequent subcultivation. Photographs were taken by using scanning electron microscopy. Result: The MICs and MBCs did not differ between ofloxacin and moxifloxacin against A. actinomycetemcomitans, moxifloxacin was more effective against anaerobes and the multi-species population. The single-species biofilms were eradicated by moderate concentrations of the antibiotics, the lowest MBECs were always determined for moxifloxacin (2-8 µg/ml). MBECs against the multi-species biofilms were 128 μg/ml, > 512 μg/ml and > 512 μg/ml for moxifloxacin, ofloxacin and doxycycline, respectively. Conclusion: Moxifloxacin is more effective against anaerobes than ofloxacin. It is able to kill bacteria within biofilms although the necessary concentration against a multi-species biofilm is high. Moxifloxacin in a topical formulation may have potential as an adjunct to mechanical removal of the biofilms. The present study was supported in part by Bayer Innovation GmbH, Leverkusen, Germany. The authors declare that they have no conflicts of interest.
    Annual Meeting of the IADR Continental European Division 2013; 09/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Rheumatoid arthritis (RA) frequently affects the temporomandibular joint (TMJ) and masticatory muscles. The aim of this pilot study was to evaluate the presence of temporomandibular dysfunction and orofacial pain in patients with RA in relation to their periodontal status. Method: 21 patients suffering of RA (DAS ≥ 3.2) were investigated for their periodontal condition (pocket depths, clinical attachment level, plaque and bleeding index) and TMJ affections (pain, sound, deviation, limitation). Furthermore, extra- and intraoral masticatory muscles were evaluated for pain at pressure; occlusal relationship, interferences and premature contacts were registered. Result: Thirteen patients (62%, mean age 52.61±11.25, 11 women) were diagnosed with moderate to severe forms of chronic periodontitis. There were no significant statistical differences regarding the number, sex, age, oral hygiene status of the patients in both groups (RA group, RA and periodontitis group). 69% of the patients with periodontitis and RA and 62.5% of the RA patients were diagnosed with discal displacement. 52% of the patients exhibited articular pain at the TMJ palpation, the majority being in the periodontitis group (64%); 45% of the periodontitis patients also suffered of muscular pain. Most patients (76.2%) had pain at the intraoral palpation of the masticatory muscles, 11 (52%) also showing pain at the extraoral palpation. Furthermore 62% of the patients (85% with periodontitis) presented TMJ sounds during mouth opening. Interferences or premature contacts were registered in all patients. Conclusion: Patients with RA and periodontitis showed significantly more symptoms for orofacial pain and affections of the TMJ as compared to patients suffering only of RA. The early diagnosis and treatment of periodontitis might prevent the development of TMJ dysfunction and orofacial pain.
    Annual Meeting of the IADR Continental European Division 2013; 09/2013
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this study is to evaluate the effects of a paste-like bone substitute material with easy handling properties and improved mechanical stability on periodontal regeneration of intrabony defects in dogs. Mandibular and maxillary first and third premolars were extracted, and three-wall intrabony defects were created on second and fourth premolars. After a healing period of 3 months, acute type defects were filled with a paste-like formulation of deproteinized bovine bone mineral (DBBM) (particle size, 0.125-0.25 mm) in a collagenous carrier matrix (T1), pulverized DBBM (particle size, 0.125-0.25 mm) without the carrier (T2), or Bio-Oss® granules (particle size, 0.25-1.00 mm) as control (C). All defects were covered with a Bio-Gide® membrane. The dogs were sacrificed after 12 weeks, and the specimens were analyzed histologically and histometrically. Postoperative healing of all defects was uneventful, and no histological signs of inflammation were observed in the augmented and gingival regions. New cementum, new periodontal ligament, and new bone were observed in all three groups. The mean vertical bone gain was 3.26 mm (T1), 3.60 mm (T2), and 3.81 mm (C). That of new cementum was 2.25 mm (T1), 3.88 mm (T2), and 3.53 mm (C). The differences did not reach statistical significance. The DBBM particles were both incorporated in new bone and embedded in immature bone marrow. The results of this preclinical study showed that the 0.125-0.25-mm DBBM particles in a powder or paste formulation resulted in periodontal regeneration comparable to the commercially available DBBM. Osteoconductivity, in particular, was not affected by DBBM size or paste formulation. The improved handling properties of the paste-like bone substitute consisting of small DBBM particles embedded in a collagen-based carrier hold promise for clinical applications.
    Clinical Oral Investigations 08/2013; · 2.20 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Guided tissue regeneration (GTR) with bioabsorbable collagen membranes (CM) is commonly used for the treatment of periodontal defects. The objective of this systematic review of randomized clinical trials was to assess the clinical efficacy of GTR procedures with CM, with or without bone substitutes, in periodontal infrabony defects compared with that of open flap debridement (OFD) alone. Primary outcomes were tooth loss and gain in clinical attachment level (CAL). Screening of records, data extraction, and risk-of-bias assessments were performed by two reviewers. Weighted mean differences were estimated by random effects meta-analysis. We included 21 reports on 17 trials. Risk of bias was generally high. No data were available for the primary outcome tooth loss. The summary treatment effect for change in CAL for GTR with CM compared with OFD was 1.58 mm (95% CI, 1.27 to 1.88). Despite large between-trial heterogeneity (I(2) = 75%, p < .001), all trials favored GTR over OFD. No differences in treatment effects were detected between trials of GTR with CM alone and trials of GTR with CM in combination with bone substitutes (p for interaction, .31). GTR with CM, with or without substitutes, may result in improved clinical outcomes compared with those achieved with OFD alone. Our findings support GTR with CM for the treatment of infrabony periodontal defects.
    Journal of dental research 07/2013; · 3.46 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND AND OBJECTIVE: Epidemiologic and clinical studies have indicated that diabetes is a risk factor for periodontal disease progression and healing. The aim of the present study was to evaluate short-term healing after enamel matrix derivative (EMD) application in combined supra/infrabony periodontal defects in diabetic rats. MATERIAL AND METHODS: Thirty male Wistar rats were initially divided into two groups, one with streptozotocin-induced diabetes and another one with healthy (non-diabetic) animals. Bony defects were surgically created on the mesial root of the first maxillary molars. After root surface planing and EDTA conditioning, EMD was applied to the roots at one side of the maxillae, while those on the contralateral sides were left untreated. Animals were killed 3 wk after surgery, and block sections were prepared for histologic and histomorphometric analysis. RESULTS: There was statistically significant more gingival recession in diabetic animals than in non-diabetic animals. The length of the junctional epithelium was significantly shorter in the EMD-treated sites in both diabetic and normoglycemic rats. Sulcus depth and length of supracrestal soft connective tissue showed no statistically significant differences between groups. In all animals, new bone formation was observed. Although new bone occurred more frequently in healthy animals, the extent of new bone was not significantly different between groups. In none of the teeth, a layer of new cementum was detectable. EMD had no influence on bone or cementum regeneration. Adverse reactions such as excessive inflammation due to bacterial root colonization, ankylosis and bone fractures were exclusively observed in diabetic animals, irrespective of EMD treatment. CONCLUSION: Within the limits of the present study, it can be concluded that periodontal healing was impaired in streptozotocin-induced diabetic rats. EMD had no beneficial effects on new bone and cementum formation during short-term healing in this defect model and could not ameliorate the adverse effects in the systemically compromised animals.
    Journal of Periodontal Research 04/2013; · 1.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: The aim of this double-blind, controlled, cross-over study was to evaluate the effect of a zinc containing glass-ionomer cement on the in situ biofilm formation and to compare it to enamel and a glass ionomer cement without zinc ions Method: Fifteen volunteers wore individual acrylic appliances in which six sterilised discs were inserted: two of bovine enamel, two of GIC ChemFil and two of GIC Equia to build up intra-oral biofilms. During two test cycles (48 hours and 96 hours) the subjects had to wear the appliances continuously. After each test cycle the developed biofilms were removed from the splint, the adhering biofilm was detached from the slab with a sterile probe and streaked on an object slide. Then the biofilm was vital stained based on the use of fluoresceindiacetate and ethidium bromide. Finally an image analysis software (AxioVision 4, Carl Zeiss, Göttingen, Germany) discriminating between green and red pixels was used to calculate the vitality of the bacterial biofilm flora, which means the percentage of vital bacteria in the total flora (VF%). Result: On enamel the highest vitality amounts (90.01±6.65 and 81.42±18.40) were detected which were not different at both time points (p=0.100). The glass ionomer cement without zinc (Equia) exerted lower vitality values both after 48 hours (83.66±7.29) and after 96 hours (74.04±13.04) compared to enamel. Only after 48 hours the reduction was significantly different compared to enamel (p=0.046). The glass ionomer with zinc ions (ChemFil) revealed the lowest values after 48 and 96 hours (74.86±6.25 and 61.36±17.81). The reductions compared to enamel were significant at all time points (p<0.001 and 0.008). Compared to Equia a significant difference was detected after 48 hours (p=0.004). Conclusion: A significant antibacterial effect of the glass ionomer cement with zinc ions could be seen compared to enamel.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate the effects of the novel erythritol powder (EPAP) on clinical and microbiological outcomes when compared to the standard care of hand instrumentation during supportive periodontal therapy (SPT). Method: 40 recall patients were randomly assigned to two groups. Bleeding on probing positive (BoP+) sites with probing pocket depth (PPD) of 4mm but no detectable calculus were defined as study sites. During standard SPT, these sites were either treated by subgingival erythritol powder air-polishing (EPAP) or scaling and root planning (SRP) at baseline (BL) and at the thee-month recall (3M). Recording of the clinical variables Plaque, BoP, PPD and clinical attachment level (CAL) as well as microbiological analysis of subgingival samples was performed at BL, 3M and 6 month (6M) after treatment Result: Both treatment methods resulted in significant reductions of BoP+ sites (EPAP: 40.45%; SRP: 42.53%), a mean reduction of the PPD (EPAP: -0.67; SRP: -0.68) and increased CAL (EPAP: 0.48; SRP: 0.61) at the six-month evaluation at study sites. There was no statistical difference observed between the treatment procedures at any of the time-points. The microbiological evaluation revealed minor shifts in the composition of the subgingival plaque, which did not involve the major periodontal pathogens. No adverse events were observed or reported by patients or observed by examiners. Conclusion: This six-month study demonstrated comparable improvements of the clinical variables with no significant differences in the clinical and microbiological treatment outcomes when comparing EPAP and hand instrumentation for patients in periodontal maintenance.
    03/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: A relationship between rheumatoid arthritis (RA) and periodontitis is suggested. Pathogenesis of periodontitis is thought to be host response on subgingival plaque. Among species associated with severe periodontitis, Porphyromonas gingivalisis important. Its virulence is related to cysteine proteases. Moreover, a peptidylarginine deiminase was described to citrullinate microbial and host proteins. The aim of this study was to characterize a group of RA patients for several variables associated with RA and / or periodontitis in comparison with periodontally healthy and periodontitis subjects without RA. In a first part, clinical data of periodontitis and the load of selected periodontopathic species were analyzed. Methods: 51 RA-patients, 27 patients with periodontitis and without RA and 16 subjects without periodontitis and RA were recruited. Periodontal disease status was determined by Periodontal Screening index (PSI). Subgingival plaque was analyzed semi-quantitatively by PCR followed by a reverse hybridization (microIdent, Hain Lifescience) for Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Real-time PCR was used to detect low loads of P. gingivalis(detection level 10 bacteria). For statistics Kruskal-Wallis and Mann-Whitney tests were used. Results: Among the 51 RA patients, 45 were characterized positively for periodontitis. 18 (35%) had severe periodontitis (PSI 4). Analyzing subgroups (incl. RA with / without periodontitis) showed differences in A. actinomycetemcomitans (p=0.043) and T. denticola (p=0.028). P. gingivalis was detected in 63% of the RA patients and in 49% of the subjects without RA. In all RA patients and in special without periodontitis, A. actinomycetemcomitans was found more often (p=0.018 for all, p=0.007 for subjects without periodontitis) than without RA. In RA and non-RA subjects, patients with periodontitis had more T. denticola(p=0.026; p=0.042). Conclusion: RA is strongly associated with periodontitis. Except for P. gingivalis, the presence of A. actinomycetemcomitans may play a role in pathogenesis of RA.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate the impact of cigarette smoking on compliance with scheduled maintenance visits for supportive periodontal therapy (SPT) provided by dental hygiene students at the Medi School of Dental Hygiene (MSDH), Bern, Switzerland. Method: Retrospective patient-related demographic data was gathered from clinical records of patients undergoing dental hygiene treatment at the MSDH over the years 1985 to 2011. All patients aged 20 years or older were included in the analysis. Patient-level compliance was calculated as a percentage of completed and expected visits. The effects of smoking on mean compliance were studied using one-way ANOVA. To adjust for potential confounding effects, gender, age, general health status, smoking status, periodontal status, duration of active periodontal therapy, duration of SPT, and SPT interval were added as covariables in a general linear model for mean compliance. All models for mean compliance were calculated using both weighted (duration of SPT) and unweighted observations. Result: A total of 1’336 patients and 12’074 SPT visits were identified from the clinical records. 25.6% of the patients never returned for SPT. Of those returning, 30.0% were smokers, 25.5% former smokers, and 44.5% non-smokers. Overall compliance was 67.2%, while smokers complied with 63.5% (SD±25.0), former smokers with 66.4% (SD±26.0), and non-smokers with 70.0% (SD±28.8) of their suggested intervals, reaching statistical significance with and without weighted observations. When adjusting for potential confounders, however, the effect of smoking was no longer statistically significant. In the weighted models, longer SPT intervals (p<0.0001), older age (p<0.0001), female gender (p<0.0127), and former smoking (p=0.0242) had greater positive impact on mean compliance than smoking (p=0.8162). Conclusion: It is concluded from this study that the inhibitory effect of smoking on mean compliance can mainly be attributed to confounding effects. Under simultaneous consideration of confounding, the effect of smoking on patient compliance vanishes.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate clinically the results following treatment of deep intrabony defects with either platelet-rich gel (PRG) or an enamel matrix derivative (EMD). Method: Twenty four patients suffering from advanced chronic periodontitis, and each of whom displayed one advanced intrabony defect were randomly treated with either PRG (test) or EMD (Emdogain, Straumann, Basel, Switzerland) (control). Clinical parameters were evaluated at baseline and at 1 year after treatment. The primary outcome variable was the clinical attachment level (CAL). Result: The test sites showed a reduction in mean probing depth (PD) from 8.8 ± 1.9 mm to 4.4 ± 0.9 mm (p<0.001) at 1 year. In the control group mean PD was reduced from 8.9 ± 1.2 mm to 4.5 ± 0.8 mm (p<0.001). In the test group mean CAL changed from 9.8 ± 2.6 mm to 6.4 ± 1.9 mm at 1 year (p<0.001). In the control group mean CAL changed from 9.88 ± 2.0 mm to 6.4 ± 1.9 mm (p<0.001). No statistically significant differences in any of the investigated parameters were observed between the two groups at 1 year. Conclusion: The present study has shown that (i) 1 year after regenerative surgery, both treatments resulted in statistically significant PD reductions and CAL gains and (ii) PRG and EMD may result in comparable clinical outcomes.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
  • A. SCULEAN
    03/2013

Publication Stats

1k Citations
106.62 Total Impact Points

Institutions

  • 2014
    • University of Münster
      Muenster, North Rhine-Westphalia, Germany
  • 2011–2014
    • Universität Bern
      • • School of Dental Medicine
      • • Department of Periodontology and Fixed Prosthodontics
      Berna, Bern, Switzerland
  • 2013
    • Philipps University of Marburg
      Marburg, Hesse, Germany
  • 2009
    • Semmelweis University
      • Department of Periodontology
      Budapeŝto, Budapest, Hungary
  • 2006–2008
    • Radboud University Medical Centre (Radboudumc)
      Nymegen, Gelderland, Netherlands
    • Heinrich-Heine-Universität Düsseldorf
      • Poliklinik für Zahnärztliche Chirurgie und Aufnahme
      Düsseldorf, North Rhine-Westphalia, Germany
  • 2005
    • Radboud University Nijmegen
      • Department of Periodontology and Biomaterials
      Nijmegen, Provincie Gelderland, Netherlands
  • 2003–2004
    • Johannes Gutenberg-Universität Mainz
      Mayence, Rheinland-Pfalz, Germany
  • 1998–2004
    • Universität des Saarlandes
      Saarbrücken, Saarland, Germany
    • Aarhus University
      • Department of Dentistry
      Aarhus, Central Jutland, Denmark